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The map label for this gene is dus1 [H]

Identifier: 39996108

GI number: 39996108

Start: 1085392

End: 1086369

Strand: Reverse

Name: dus1 [H]

Synonym: GSU1005

Alternate gene names: 39996108

Gene position: 1086369-1085392 (Counterclockwise)

Preceding gene: 39996110

Following gene: 39996107

Centisome position: 28.48

GC content: 65.24

Gene sequence:

>978_bases
ATGATCCGTTCTCTTACCATAGGCTCCCTGACCCTGGGAAACAATCTCATCCTCGCCCCCATGGCCGGGATAACCAACCT
TCCCTTCCGCCTCCTGGCCCGCGAACAGGGGGCAGGTCTCTGCTTCACCGAGATGGTGAGCGTGAACGGCCTTGTCCGGG
AAGGTAAAAAGAGTTTCGAACTCCTGCGGAGCGTGCCGGGGGATCGCCCCCTCGGCATCCAGCTTTTCGGGGACGACCCG
GACGTCATGGGCCGGGTGGCGGCCACTGTGGACGGATACGGGGACCTCATCGACATCAACATGGGATGCCCCGTGAAGAA
GGTGGTGGGGACCGGCGCCGGGAGCGCCCTCATGCGTGAGCCGGACAAGGTGCGGGCCATTGTCAGGGCCGTCCGGCGAG
CCACGCGGCTGCCGCTGACCGTGAAGATCAGGAGCGGGTGGAGCTGCGAAGATGCCAACTTTATCCAGATTGCCCGGATT
GCCGAGGAAGAGGGATGCAATGCAGTTACGCTCCATCCCCGGAGCAGGGCACAGATGTTCGAAGGCACGGCCGACTGGAC
GAAGCTCGCCGAACTGAAGCAGGCCGTCGCCATACCGGTCATCGGCAGCGGCGACCTCTTCAGCGCGGCCGACGTGGCCG
CCATGCTCGACCGGACCGGCTGCGACGGGGTCATGATCGCCCGAGGTGCTCTGGGAAATCCGTGGATCTTCAGGCAGGCC
CTGGACCTGATGGCCGGACGCGAGCCGGCGGCGGCCTCCCCGGCCGAACGGTTGGCGGTGGCCCGGAGGCATCTGGCCCT
GTTCACGGAAATGGCCGGCGAACGGGTAGCCGCGAGAGAGATGCGCAAGCACCTGGGGTGGTACTCCCACGGACTCCCCG
GTGCGGCACAGTTCCGGAAGGAAATCAACGAGATTGAGGGCAATGGCGCCCTGATGGAAGCAGTGAGCCGCTTTTTCACG
GCTGTGGGGGCGCCATGA

Upstream 100 bases:

>100_bases
CCGGCGACGTTGGACAACACCAAAATAAAAGCTTGCGTTTTTCCGGCCCCATGCTACTATGCCTAAATTTTAAGCAATAA
AATCGTGCGATCGCCAACGA

Downstream 100 bases:

>100_bases
CCACCGAAGAAAAGAAAGAGGAATTCCTTGCCACCGTGATCGACAGCGTCGGAGACGGCGTCATCGTCATCGACCTGGAC
GGACGGATCGCCCTGATGAA

Product: dihydrouridine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MIRSLTIGSLTLGNNLILAPMAGITNLPFRLLAREQGAGLCFTEMVSVNGLVREGKKSFELLRSVPGDRPLGIQLFGDDP
DVMGRVAATVDGYGDLIDINMGCPVKKVVGTGAGSALMREPDKVRAIVRAVRRATRLPLTVKIRSGWSCEDANFIQIARI
AEEEGCNAVTLHPRSRAQMFEGTADWTKLAELKQAVAIPVIGSGDLFSAADVAAMLDRTGCDGVMIARGALGNPWIFRQA
LDLMAGREPAAASPAERLAVARRHLALFTEMAGERVAAREMRKHLGWYSHGLPGAAQFRKEINEIEGNGALMEAVSRFFT
AVGAP

Sequences:

>Translated_325_residues
MIRSLTIGSLTLGNNLILAPMAGITNLPFRLLAREQGAGLCFTEMVSVNGLVREGKKSFELLRSVPGDRPLGIQLFGDDP
DVMGRVAATVDGYGDLIDINMGCPVKKVVGTGAGSALMREPDKVRAIVRAVRRATRLPLTVKIRSGWSCEDANFIQIARI
AEEEGCNAVTLHPRSRAQMFEGTADWTKLAELKQAVAIPVIGSGDLFSAADVAAMLDRTGCDGVMIARGALGNPWIFRQA
LDLMAGREPAAASPAERLAVARRHLALFTEMAGERVAAREMRKHLGWYSHGLPGAAQFRKEINEIEGNGALMEAVSRFFT
AVGAP
>Mature_325_residues
MIRSLTIGSLTLGNNLILAPMAGITNLPFRLLAREQGAGLCFTEMVSVNGLVREGKKSFELLRSVPGDRPLGIQLFGDDP
DVMGRVAATVDGYGDLIDINMGCPVKKVVGTGAGSALMREPDKVRAIVRAVRRATRLPLTVKIRSGWSCEDANFIQIARI
AEEEGCNAVTLHPRSRAQMFEGTADWTKLAELKQAVAIPVIGSGDLFSAADVAAMLDRTGCDGVMIARGALGNPWIFRQA
LDLMAGREPAAASPAERLAVARRHLALFTEMAGERVAAREMRKHLGWYSHGLPGAAQFRKEINEIEGNGALMEAVSRFFT
AVGAP

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI40807366, Length=312, Percent_Identity=31.4102564102564, Blast_Score=138, Evalue=6e-33,
Organism=Homo sapiens, GI239788483, Length=260, Percent_Identity=35.7692307692308, Blast_Score=137, Evalue=9e-33,
Organism=Homo sapiens, GI239788462, Length=242, Percent_Identity=36.7768595041322, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI31742496, Length=222, Percent_Identity=31.0810810810811, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI8923374, Length=335, Percent_Identity=28.6567164179104, Blast_Score=110, Evalue=1e-24,
Organism=Escherichia coli, GI1789660, Length=321, Percent_Identity=41.7445482866044, Blast_Score=224, Evalue=6e-60,
Organism=Escherichia coli, GI1788462, Length=230, Percent_Identity=30.8695652173913, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI145693211, Length=308, Percent_Identity=26.2987012987013, Blast_Score=79, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17543114, Length=245, Percent_Identity=31.4285714285714, Blast_Score=124, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI25144369, Length=296, Percent_Identity=29.0540540540541, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17507177, Length=245, Percent_Identity=32.2448979591837, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17510279, Length=248, Percent_Identity=29.8387096774194, Blast_Score=88, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6323560, Length=277, Percent_Identity=26.3537906137184, Blast_Score=110, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323433, Length=294, Percent_Identity=27.2108843537415, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6323437, Length=226, Percent_Identity=28.7610619469027, Blast_Score=100, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24585320, Length=247, Percent_Identity=35.2226720647773, Blast_Score=141, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24580595, Length=225, Percent_Identity=34.2222222222222, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI19920448, Length=225, Percent_Identity=34.2222222222222, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI19921524, Length=225, Percent_Identity=33.3333333333333, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI45549423, Length=248, Percent_Identity=31.4516129032258, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 34880; Mature: 34880

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRSLTIGSLTLGNNLILAPMAGITNLPFRLLAREQGAGLCFTEMVSVNGLVREGKKSFE
CCCEEEECEEEECCCEEEECCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
LLRSVPGDRPLGIQLFGDDPDVMGRVAATVDGYGDLIDINMGCPVKKVVGTGAGSALMRE
HHHHCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHCCCCCHHHHCC
PDKVRAIVRAVRRATRLPLTVKIRSGWSCEDANFIQIARIAEEEGCNAVTLHPRSRAQMF
CHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHEEHHHHCCCCEEEECCCHHHHHH
EGTADWTKLAELKQAVAIPVIGSGDLFSAADVAAMLDRTGCDGVMIARGALGNPWIFRQA
CCCHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHH
LDLMAGREPAAASPAERLAVARRHLALFTEMAGERVAAREMRKHLGWYSHGLPGAAQFRK
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHH
EINEIEGNGALMEAVSRFFTAVGAP
HHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIRSLTIGSLTLGNNLILAPMAGITNLPFRLLAREQGAGLCFTEMVSVNGLVREGKKSFE
CCCEEEECEEEECCCEEEECCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
LLRSVPGDRPLGIQLFGDDPDVMGRVAATVDGYGDLIDINMGCPVKKVVGTGAGSALMRE
HHHHCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHCCCCCHHHHCC
PDKVRAIVRAVRRATRLPLTVKIRSGWSCEDANFIQIARIAEEEGCNAVTLHPRSRAQMF
CHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHEEHHHHCCCCEEEECCCHHHHHH
EGTADWTKLAELKQAVAIPVIGSGDLFSAADVAAMLDRTGCDGVMIARGALGNPWIFRQA
CCCHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHH
LDLMAGREPAAASPAERLAVARRHLALFTEMAGERVAAREMRKHLGWYSHGLPGAAQFRK
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHH
EINEIEGNGALMEAVSRFFTAVGAP
HHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]