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The map label for this gene is mutM

Identifier: 39996100

GI number: 39996100

Start: 1076875

End: 1077690

Strand: Reverse

Name: mutM

Synonym: GSU0997

Alternate gene names: 39996100

Gene position: 1077690-1076875 (Counterclockwise)

Preceding gene: 39996105

Following gene: 39996097

Centisome position: 28.26

GC content: 66.79

Gene sequence:

>816_bases
ATGCCGGAGTTGCCGGAAGTGGAGACAACCCTGCGGGGGATCGCGCCCCATGTGACCGGGCGGCGGGTGACGGCGGTCAC
GGCCCGGGCAGCCAAGCTGCGGTTGCCGATTCCCCCCGAGTTGGGCGAGCGGCTCACCGGCAGGGTCATCGAACGGGTGG
AGCGCCGGGCCAAGTATCTCCTCTTGCGCTGCGGCGACGGCACGGCCATTATCCACCTGGGGATGACCGGCACGCTCCGG
GTCGCTCCGGCAGGCAGTCCGCCGGGAAAGTACGACCACCTGGACCTGGTCCTGGACGACGGCCGCACCCTCCGCTTCAG
GGATCCGCGCAAATTCGGGCTCGTCCTCTGGACCGGCAGCGATCCCCTTGCCCACCCGCTACTGGCACAACTGGGGCCGG
AGCCTTTCCCGCCCCTGTTCAACGGGAGCTATCTCTTCTCGCGCAGCCGGAAGCGGAATGCCGCCATCAAGCTTCTGCTC
ATGGACAACCGGATCGTGGTCGGCGTGGGGAACATCTACGCCAATGAGGCCCTGTTCCGGGCGCGCATCCATCCCGAACG
CGCCGCGGGGTCACTCTCTGAGGAAGACTGCGCCACGTTGGCAACCGCCGTCGGGGATGTGCTGCGCGACGCCATTGCCG
AAGGAGATACAACCCTCCACGAATTCATCGCGACCGAGGTTCCTTCGGGGTATTTCCGCATCAATCCGGCCGTCTACGGA
CAGACCGGCAAACCGTGCACGGTCTGCGGCACGCCCATCGCCCGGATCCGGCTCGGCAACCGCTCAACCTGGTTCTGCCC
CGTCTGCCAGAAATGA

Upstream 100 bases:

>100_bases
GTCGGAGGAAGGGGTACTATACCCTGCGCCGCCGGTCCGGGTCAACCGTTTTGACCGGGCCGGCGGCGCGCCGGAATAAT
GCGGATCAGCGGGAGGATAG

Downstream 100 bases:

>100_bases
AAAAAGCCGGCTGTGCCGGCTTGACCGGGACGCAGTCGCGGACGGTCAGTTACAGTCGTCGCGCCAGAGACAGCTATCCT
GCCCGCACGAGTCGGCGCGG

Product: formamidopyrimidine-DNA glycosylase

Products: NA

Alternate protein names: Fapy-DNA glycosylase; DNA-(apurinic or apyrimidinic site) lyase mutM; AP lyase mutM

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MPELPEVETTLRGIAPHVTGRRVTAVTARAAKLRLPIPPELGERLTGRVIERVERRAKYLLLRCGDGTAIIHLGMTGTLR
VAPAGSPPGKYDHLDLVLDDGRTLRFRDPRKFGLVLWTGSDPLAHPLLAQLGPEPFPPLFNGSYLFSRSRKRNAAIKLLL
MDNRIVVGVGNIYANEALFRARIHPERAAGSLSEEDCATLATAVGDVLRDAIAEGDTTLHEFIATEVPSGYFRINPAVYG
QTGKPCTVCGTPIARIRLGNRSTWFCPVCQK

Sequences:

>Translated_271_residues
MPELPEVETTLRGIAPHVTGRRVTAVTARAAKLRLPIPPELGERLTGRVIERVERRAKYLLLRCGDGTAIIHLGMTGTLR
VAPAGSPPGKYDHLDLVLDDGRTLRFRDPRKFGLVLWTGSDPLAHPLLAQLGPEPFPPLFNGSYLFSRSRKRNAAIKLLL
MDNRIVVGVGNIYANEALFRARIHPERAAGSLSEEDCATLATAVGDVLRDAIAEGDTTLHEFIATEVPSGYFRINPAVYG
QTGKPCTVCGTPIARIRLGNRSTWFCPVCQK
>Mature_270_residues
PELPEVETTLRGIAPHVTGRRVTAVTARAAKLRLPIPPELGERLTGRVIERVERRAKYLLLRCGDGTAIIHLGMTGTLRV
APAGSPPGKYDHLDLVLDDGRTLRFRDPRKFGLVLWTGSDPLAHPLLAQLGPEPFPPLFNGSYLFSRSRKRNAAIKLLLM
DNRIVVGVGNIYANEALFRARIHPERAAGSLSEEDCATLATAVGDVLRDAIAEGDTTLHEFIATEVPSGYFRINPAVYGQ
TGKPCTVCGTPIARIRLGNRSTWFCPVCQK

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyr

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger

Homologues:

Organism=Escherichia coli, GI1790066, Length=271, Percent_Identity=49.4464944649446, Blast_Score=268, Evalue=2e-73,
Organism=Escherichia coli, GI1786932, Length=278, Percent_Identity=25.5395683453237, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FPG_GEOSL (Q74EG5)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_952051.1
- ProteinModelPortal:   Q74EG5
- SMR:   Q74EG5
- GeneID:   2685647
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU0997
- NMPDR:   fig|243231.1.peg.989
- TIGR:   GSU0997
- HOGENOM:   HBG690070
- OMA:   RMTGQLL
- BioCyc:   GSUL243231:GSU_0997-MONOMER
- BRENDA:   3.2.2.23
- BRENDA:   4.2.99.18
- HAMAP:   MF_00103
- InterPro:   IPR015886
- InterPro:   IPR015887
- InterPro:   IPR000191
- InterPro:   IPR012319
- InterPro:   IPR020629
- InterPro:   IPR010979
- InterPro:   IPR000214
- InterPro:   IPR010663
- SMART:   SM00898
- TIGRFAMs:   TIGR00577

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH; PF06827 zf-FPG_IleRS; SSF81624 Form_DNAglyc_cat; SSF46946 Ribosomal_H2TH

EC number: =3.2.2.23; =4.2.99.18

Molecular weight: Translated: 29636; Mature: 29505

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS51068 FPG_CAT; PS01242 ZF_FPG_1; PS51066 ZF_FPG_2; PS01076 ACETATE_KINASE_2

Important sites: ACT_SITE 2-2 ACT_SITE 3-3 ACT_SITE 58-58 ACT_SITE 261-261 BINDING 110-110 BINDING 152-152

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELPEVETTLRGIAPHVTGRRVTAVTARAAKLRLPIPPELGERLTGRVIERVERRAKYL
CCCCCCHHHHHHHCCCCCCCCEEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHEEE
LLRCGDGTAIIHLGMTGTLRVAPAGSPPGKYDHLDLVLDDGRTLRFRDPRKFGLVLWTGS
EEEECCCCEEEEECCCCEEEEEECCCCCCCCCEEEEEEECCCEEEEECCCEEEEEEEECC
DPLAHPLLAQLGPEPFPPLFNGSYLFSRSRKRNAAIKLLLMDNRIVVGVGNIYANEALFR
CCHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCEEEEEEECCEEEEEECCHHHCCCEEE
ARIHPERAAGSLSEEDCATLATAVGDVLRDAIAEGDTTLHEFIATEVPSGYFRINPAVYG
EECCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEECCEEEC
QTGKPCTVCGTPIARIRLGNRSTWFCPVCQK
CCCCCEEEECCCEEEEEECCCCEEEECCCCC
>Mature Secondary Structure 
PELPEVETTLRGIAPHVTGRRVTAVTARAAKLRLPIPPELGERLTGRVIERVERRAKYL
CCCCCHHHHHHHCCCCCCCCEEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHEEE
LLRCGDGTAIIHLGMTGTLRVAPAGSPPGKYDHLDLVLDDGRTLRFRDPRKFGLVLWTGS
EEEECCCCEEEEECCCCEEEEEECCCCCCCCCEEEEEEECCCEEEEECCCEEEEEEEECC
DPLAHPLLAQLGPEPFPPLFNGSYLFSRSRKRNAAIKLLLMDNRIVVGVGNIYANEALFR
CCHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCEEEEEEECCEEEEEECCHHHCCCEEE
ARIHPERAAGSLSEEDCATLATAVGDVLRDAIAEGDTTLHEFIATEVPSGYFRINPAVYG
EECCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEECCEEEC
QTGKPCTVCGTPIARIRLGNRSTWFCPVCQK
CCCCCEEEECCCEEEEEECCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA