Click here to switch to the map view.

The map label for this gene is fumB [H]

Identifier: 39996097

GI number: 39996097

Start: 1074639

End: 1076264

Strand: Reverse

Name: fumB [H]

Synonym: GSU0994

Alternate gene names: 39996097

Gene position: 1076264-1074639 (Counterclockwise)

Preceding gene: 39996100

Following gene: 39996096

Centisome position: 28.22

GC content: 62.79

Gene sequence:

>1626_bases
ATGTCCACCAAGCCGTTCGTCTACCAGGACCCTTTCCCCCTCGGCAAAGATGAGACGAAGTACCGCAAGATCGAGGGATC
CGAGAAACATGTCACGGTCGAGCAGTTCGCCGGCAAGGAAGTGCTGCGGGTCTGCCCCGAGGCGCTTTCCATCCTCGCCA
ACGAGGCGATGCGCGATGTATCCTTCCTGCTGCGCCCCGAGCACAACGAGTCGGTGGCGAAGATCCTGCGCGACCCGGAA
GCGTCCATGAACGACAAGGGCGTGGCCCTCGCATTCCTGCGCAACGCCGAGATCTCCGCCAACTTCGAGCTTCCCATCTG
CCAGGATACCGGCACCGCCACCGTCATCGCCAAGAAGGGCCAGCAGGTCTGGACCGGCGGCAAGGATGAAGAATACCTCT
CCAAGGGGGTCTACAAGACCTACACCGAGGAGAACCTCCGTTACTCCCAGACCGTTGCCCTCAACATGTACGACGAGATC
AACACCGGCACCAATCTCCCGGCCCAGGTCGAGATCCAGGCCGTGGACGGCGATGCCTACAAGTTTCTCTTCATGGCCAA
AGGTGGGGGCTCCGCCAACAAGACCATGCTTTTCCAGGAGACCAAGGCGCTGCTCACCCCCGAGAAGCTTGAAAAGTTCC
TCGTGGACAAGATGAAGTACCTGGGCACCGCCGCCTGTCCGCCCTACCACATCGCCGTGGTCGTGGGCGGCTGCTCCGCC
GACCAGTGCATGAAGACCGTCAAGCTTGCCTCGGCCAAGTACCTGGACGCACTCCCCACCCAGGGGAACGAGCACGGCCA
GGCGTTCCGCGATGTGGAGCTTGAGGAGAAACTCCTCCAGGCCGCCTACAAGCTCGGCATCGGCGCCCAGTTCGGCGGCA
AGTACTTCGCCCACGACGTCCGGGTCATCCGGCTTCCGCGTCACGGCGCTTCCTGCCCCGTGGGGATGGCGGTCTCCTGT
TCCGCCGACCGCAACATCAAGGCGAAGATCACCAGGGACGGCCTCTTCGTGGAAGAGCTCGACCGCAATCCGGGCCGCCT
CATCCCCGAGCAGTACCGCGGCAAGCACGAGCACGGCGTCAAGATCGACCTGAACCGCCCCATGAAGGAAATCCTCGCCG
AGCTCAGCAAGTACCCGGTTGCAACGCCGCTGCTCCTGAACGGTACCATCGTGGTCGGCCGCGACATCGCCCACGCCAAG
TTCAAGGAGATCATCGACGCCGGCAAGCCGGTGCCCGAGTACCTGCTCAACCACCCGATCTACTACGCGGGACCGGCCAA
GACCCCGGCTGGCAAGCCCTCCGGCTCCTTCGGCCCCACCACCGCCGGCCGGATGGACTCCTACGTGGATCTCCTCCAGT
CCAAGGGCGCATCCATGATCATGATCGCCAAGGGGAACCGCAGCCAGCAGGTGACCGATGCCTGCCAGAAGTACGGCGGG
TTCTACCTGGGCTCCATCGGCGGCCCGGCCGCGGTTCTTGCCGAGGAGAACATCAAGAAGGTCGAGTGCATCGACTTCCC
CGAACTGGGGATGGAAGCGGTCTGGAAGATCGAAGTGGAAAACTTCCCGGCCTTCATCCTTGTGGACGACAAGGGGAACG
ACTTCTTCAAGCAACTGGGTCTGTAA

Upstream 100 bases:

>100_bases
TGTATACACTTACGCGGACAGCATGGCCCGGAGCGTATAACGATTCCCGCCCGACCCTGCATCCCCGGCCGTCAGCCCAT
GATCGAAAGGAGAGCACGCG

Downstream 100 bases:

>100_bases
GCACTGAAGCCCACCACGCCCCCGGCCTCCTGCGGCCGGGGGCGTTTTTGCGCGTATCGGTCAATGGTAGCGGGCCCGTG
TGCCGGGGCGCCGGATGGAC

Product: fumarate hydratase, class I

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 541; Mature: 540

Protein sequence:

>541_residues
MSTKPFVYQDPFPLGKDETKYRKIEGSEKHVTVEQFAGKEVLRVCPEALSILANEAMRDVSFLLRPEHNESVAKILRDPE
ASMNDKGVALAFLRNAEISANFELPICQDTGTATVIAKKGQQVWTGGKDEEYLSKGVYKTYTEENLRYSQTVALNMYDEI
NTGTNLPAQVEIQAVDGDAYKFLFMAKGGGSANKTMLFQETKALLTPEKLEKFLVDKMKYLGTAACPPYHIAVVVGGCSA
DQCMKTVKLASAKYLDALPTQGNEHGQAFRDVELEEKLLQAAYKLGIGAQFGGKYFAHDVRVIRLPRHGASCPVGMAVSC
SADRNIKAKITRDGLFVEELDRNPGRLIPEQYRGKHEHGVKIDLNRPMKEILAELSKYPVATPLLLNGTIVVGRDIAHAK
FKEIIDAGKPVPEYLLNHPIYYAGPAKTPAGKPSGSFGPTTAGRMDSYVDLLQSKGASMIMIAKGNRSQQVTDACQKYGG
FYLGSIGGPAAVLAEENIKKVECIDFPELGMEAVWKIEVENFPAFILVDDKGNDFFKQLGL

Sequences:

>Translated_541_residues
MSTKPFVYQDPFPLGKDETKYRKIEGSEKHVTVEQFAGKEVLRVCPEALSILANEAMRDVSFLLRPEHNESVAKILRDPE
ASMNDKGVALAFLRNAEISANFELPICQDTGTATVIAKKGQQVWTGGKDEEYLSKGVYKTYTEENLRYSQTVALNMYDEI
NTGTNLPAQVEIQAVDGDAYKFLFMAKGGGSANKTMLFQETKALLTPEKLEKFLVDKMKYLGTAACPPYHIAVVVGGCSA
DQCMKTVKLASAKYLDALPTQGNEHGQAFRDVELEEKLLQAAYKLGIGAQFGGKYFAHDVRVIRLPRHGASCPVGMAVSC
SADRNIKAKITRDGLFVEELDRNPGRLIPEQYRGKHEHGVKIDLNRPMKEILAELSKYPVATPLLLNGTIVVGRDIAHAK
FKEIIDAGKPVPEYLLNHPIYYAGPAKTPAGKPSGSFGPTTAGRMDSYVDLLQSKGASMIMIAKGNRSQQVTDACQKYGG
FYLGSIGGPAAVLAEENIKKVECIDFPELGMEAVWKIEVENFPAFILVDDKGNDFFKQLGL
>Mature_540_residues
STKPFVYQDPFPLGKDETKYRKIEGSEKHVTVEQFAGKEVLRVCPEALSILANEAMRDVSFLLRPEHNESVAKILRDPEA
SMNDKGVALAFLRNAEISANFELPICQDTGTATVIAKKGQQVWTGGKDEEYLSKGVYKTYTEENLRYSQTVALNMYDEIN
TGTNLPAQVEIQAVDGDAYKFLFMAKGGGSANKTMLFQETKALLTPEKLEKFLVDKMKYLGTAACPPYHIAVVVGGCSAD
QCMKTVKLASAKYLDALPTQGNEHGQAFRDVELEEKLLQAAYKLGIGAQFGGKYFAHDVRVIRLPRHGASCPVGMAVSCS
ADRNIKAKITRDGLFVEELDRNPGRLIPEQYRGKHEHGVKIDLNRPMKEILAELSKYPVATPLLLNGTIVVGRDIAHAKF
KEIIDAGKPVPEYLLNHPIYYAGPAKTPAGKPSGSFGPTTAGRMDSYVDLLQSKGASMIMIAKGNRSQQVTDACQKYGGF
YLGSIGGPAAVLAEENIKKVECIDFPELGMEAVWKIEVENFPAFILVDDKGNDFFKQLGL

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=540, Percent_Identity=72.037037037037, Blast_Score=825, Evalue=0.0,
Organism=Escherichia coli, GI1787897, Length=542, Percent_Identity=70.479704797048, Blast_Score=810, Evalue=0.0,
Organism=Escherichia coli, GI1789443, Length=181, Percent_Identity=29.2817679558011, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1789442, Length=260, Percent_Identity=27.6923076923077, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 59436; Mature: 59305

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTKPFVYQDPFPLGKDETKYRKIEGSEKHVTVEQFAGKEVLRVCPEALSILANEAMRDV
CCCCCEEEECCCCCCCCCCHHEECCCCCCEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHH
SFLLRPEHNESVAKILRDPEASMNDKGVALAFLRNAEISANFELPICQDTGTATVIAKKG
HHHCCCCCCHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCEEECCCCCEEEEEECC
QQVWTGGKDEEYLSKGVYKTYTEENLRYSQTVALNMYDEINTGTNLPAQVEIQAVDGDAY
CEEECCCCCHHHHHCCCHHHHHHCCCEEEEEEEEEEHHHCCCCCCCCCEEEEEEECCCEE
KFLFMAKGGGSANKTMLFQETKALLTPEKLEKFLVDKMKYLGTAACPPYHIAVVVGGCSA
EEEEEECCCCCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCH
DQCMKTVKLASAKYLDALPTQGNEHGQAFRDVELEEKLLQAAYKLGIGAQFGGKYFAHDV
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCCCEEECCE
RVIRLPRHGASCPVGMAVSCSADRNIKAKITRDGLFVEELDRNPGRLIPEQYRGKHEHGV
EEEEECCCCCCCCCCEEEECCCCCCEEEEEECCCCCHHHHCCCCCCCCCHHHCCCCCCCE
KIDLNRPMKEILAELSKYPVATPLLLNGTIVVGRDIAHAKFKEIIDAGKPVPEYLLNHPI
EEECCCCHHHHHHHHHHCCCCCEEEECCEEEEECCHHHHHHHHHHHCCCCCHHHHHCCCE
YYAGPAKTPAGKPSGSFGPTTAGRMDSYVDLLQSKGASMIMIAKGNRSQQVTDACQKYGG
EEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCC
FYLGSIGGPAAVLAEENIKKVECIDFPELGMEAVWKIEVENFPAFILVDDKGNDFFKQLG
EEEECCCCCEEEEECCCCCEEEECCCCCCCCCEEEEEEECCCCEEEEEECCCCHHHHHHC
L
C
>Mature Secondary Structure 
STKPFVYQDPFPLGKDETKYRKIEGSEKHVTVEQFAGKEVLRVCPEALSILANEAMRDV
CCCCEEEECCCCCCCCCCHHEECCCCCCEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHH
SFLLRPEHNESVAKILRDPEASMNDKGVALAFLRNAEISANFELPICQDTGTATVIAKKG
HHHCCCCCCHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCEEECCCCCEEEEEECC
QQVWTGGKDEEYLSKGVYKTYTEENLRYSQTVALNMYDEINTGTNLPAQVEIQAVDGDAY
CEEECCCCCHHHHHCCCHHHHHHCCCEEEEEEEEEEHHHCCCCCCCCCEEEEEEECCCEE
KFLFMAKGGGSANKTMLFQETKALLTPEKLEKFLVDKMKYLGTAACPPYHIAVVVGGCSA
EEEEEECCCCCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCH
DQCMKTVKLASAKYLDALPTQGNEHGQAFRDVELEEKLLQAAYKLGIGAQFGGKYFAHDV
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCCCEEECCE
RVIRLPRHGASCPVGMAVSCSADRNIKAKITRDGLFVEELDRNPGRLIPEQYRGKHEHGV
EEEEECCCCCCCCCCEEEECCCCCCEEEEEECCCCCHHHHCCCCCCCCCHHHCCCCCCCE
KIDLNRPMKEILAELSKYPVATPLLLNGTIVVGRDIAHAKFKEIIDAGKPVPEYLLNHPI
EEECCCCHHHHHHHHHHCCCCCEEEECCEEEEECCHHHHHHHHHHHCCCCCHHHHHCCCE
YYAGPAKTPAGKPSGSFGPTTAGRMDSYVDLLQSKGASMIMIAKGNRSQQVTDACQKYGG
EEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCC
FYLGSIGGPAAVLAEENIKKVECIDFPELGMEAVWKIEVENFPAFILVDDKGNDFFKQLG
EEEECCCCCEEEEECCCCCEEEECCCCCCCCCEEEEEEECCCCEEEEEECCCCHHHHHHC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]