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The map label for this gene is macA [H]

Identifier: 39996052

GI number: 39996052

Start: 1021297

End: 1022469

Strand: Reverse

Name: macA [H]

Synonym: GSU0949

Alternate gene names: 39996052

Gene position: 1022469-1021297 (Counterclockwise)

Preceding gene: 39996053

Following gene: 39996051

Centisome position: 26.81

GC content: 70.5

Gene sequence:

>1173_bases
ATGACGTTGATACACACACTGCGCAGACACAAGACCGCCCTGGTCCGGACCGCACTTCTCGCGGCGCTGGTCGTGCTCGT
CAAGGCCACCATCCTGGCCCCGCCGAAGGTCACGACGGTACGGGTCGAACGGCGGGACCTGACGGCCCGGGTCTATGGCA
ACGGGACCGTGGAGGCCAAGGAGGTGGTGGGGGTTTCCAGCAAGGTGACCGGAAGGATCGTTTCGCTCCACGCTGACCAG
GGGGACCGGGTGCGCCGGGGCCAGATCCTGGCGCGCCTGGAAAGCGACGAATATGCCGCGCAGCTGGGTCAAGCCGAAGC
AGGGGTCAGCCGGGCGGCGGCCGGCCAGAGCCTAGAGGCGGCCACCCTGGCAAAGGCGCGGGCAAACCTGGAACTGGCCG
AGCGTAACGCTGCGCGCTACCGGAATCTGGCCGACCGGAACCTGGTGTCGCGCCAGGAAGCCGAGCAGTACGAAACGGCC
TGGCGCCTGGCACGGGAGGAGGAAGCCCGGAGCAGTGCGGCCCTGGAAGCGGCCCGCATGGAGTCGGCGGCCGGACGCGC
TGCCCGGGGAGTGGCCCGGTCGCGGCTTGACGACACCGTTATCCGCGCCCCCCGCGACGGGATCATCGTCTCCCGCGACC
TGGAAAAGGGGGCCGTGGTCTCTCCCGGCCAGAGCATCTTCACCCTTGCCGATCCGGCGGTGGTCTGGGTCAGGGCCCAT
GTGGACGAATCGCAGCTTGCCGGCGTGGCCGTGGGCCGGGAGGCCGCGATCTCCCTCCGGTCCGCGCCGGAGCGTGCCAT
GCAGGGACGGGTGGCCCGGGTCGGCATGGAGAGCGACCGGGTGACTGAAGAACTGGCGGTGGACGTGGCCTTCGCCGCCC
CGCCGCCCGCGTTCCGCCTGGGAGAGCAGTCCGACGTTCTGATCACGGTCGGCACCAAACGCGGCGTGCCGTCCCTGCCT
GCCGCGGCCCTGACGAGCCACGGGGGGAAGCACGGGGTCTGGACGGTGTCGGAAGGTCGGCTCCGCTTCAAGCCGGTGCA
GGTCGGCATCGAGGATCGCCAGGGCGTCACCGAGATCCTGTCGGGCCTGGCCGGGGCCGAGCCGGTGGTGCTGCCGACGC
CGGCCCAGGCGAAGAAATTCCGTGACGGCATGAAGGTGAGGGTGTCGCCGTGA

Upstream 100 bases:

>100_bases
AGCTGGAAGCGGAAACGGCCAACATCCGCGCCAGGGGTGATACTCGCATAGCCTTGGCGCGTCTCGATCGGGCGGTTGGC
GACGACGGAGGAACGGAACA

Downstream 100 bases:

>100_bases
ACCTGGCGGTCCGCGACATCCGCTGCCACCGGGGGCGTTTCGTCCAGACCTGCCTGGGGCTGGGGCTGCTCCTGGCCGTG
GTCATGAGCATGGGGGGGAT

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MTLIHTLRRHKTALVRTALLAALVVLVKATILAPPKVTTVRVERRDLTARVYGNGTVEAKEVVGVSSKVTGRIVSLHADQ
GDRVRRGQILARLESDEYAAQLGQAEAGVSRAAAGQSLEAATLAKARANLELAERNAARYRNLADRNLVSRQEAEQYETA
WRLAREEEARSSAALEAARMESAAGRAARGVARSRLDDTVIRAPRDGIIVSRDLEKGAVVSPGQSIFTLADPAVVWVRAH
VDESQLAGVAVGREAAISLRSAPERAMQGRVARVGMESDRVTEELAVDVAFAAPPPAFRLGEQSDVLITVGTKRGVPSLP
AAALTSHGGKHGVWTVSEGRLRFKPVQVGIEDRQGVTEILSGLAGAEPVVLPTPAQAKKFRDGMKVRVSP

Sequences:

>Translated_390_residues
MTLIHTLRRHKTALVRTALLAALVVLVKATILAPPKVTTVRVERRDLTARVYGNGTVEAKEVVGVSSKVTGRIVSLHADQ
GDRVRRGQILARLESDEYAAQLGQAEAGVSRAAAGQSLEAATLAKARANLELAERNAARYRNLADRNLVSRQEAEQYETA
WRLAREEEARSSAALEAARMESAAGRAARGVARSRLDDTVIRAPRDGIIVSRDLEKGAVVSPGQSIFTLADPAVVWVRAH
VDESQLAGVAVGREAAISLRSAPERAMQGRVARVGMESDRVTEELAVDVAFAAPPPAFRLGEQSDVLITVGTKRGVPSLP
AAALTSHGGKHGVWTVSEGRLRFKPVQVGIEDRQGVTEILSGLAGAEPVVLPTPAQAKKFRDGMKVRVSP
>Mature_389_residues
TLIHTLRRHKTALVRTALLAALVVLVKATILAPPKVTTVRVERRDLTARVYGNGTVEAKEVVGVSSKVTGRIVSLHADQG
DRVRRGQILARLESDEYAAQLGQAEAGVSRAAAGQSLEAATLAKARANLELAERNAARYRNLADRNLVSRQEAEQYETAW
RLAREEEARSSAALEAARMESAAGRAARGVARSRLDDTVIRAPRDGIIVSRDLEKGAVVSPGQSIFTLADPAVVWVRAHV
DESQLAGVAVGREAAISLRSAPERAMQGRVARVGMESDRVTEELAVDVAFAAPPPAFRLGEQSDVLITVGTKRGVPSLPA
AALTSHGGKHGVWTVSEGRLRFKPVQVGIEDRQGVTEILSGLAGAEPVVLPTPAQAKKFRDGMKVRVSP

Specific function: Efflux transporter for macrolide antibiotics [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=371, Percent_Identity=25.6064690026954, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87082036, Length=333, Percent_Identity=28.2282282282282, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787013, Length=255, Percent_Identity=28.6274509803922, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790519, Length=246, Percent_Identity=28.0487804878049, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1789900, Length=245, Percent_Identity=28.5714285714286, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87081951, Length=222, Percent_Identity=27.4774774774775, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41669; Mature: 41538

Theoretical pI: Translated: 10.83; Mature: 10.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLIHTLRRHKTALVRTALLAALVVLVKATILAPPKVTTVRVERRDLTARVYGNGTVEAK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEECCCCEEHH
EVVGVSSKVTGRIVSLHADQGDRVRRGQILARLESDEYAAQLGQAEAGVSRAAAGQSLEA
HHHCCCCCCCCEEEEEECCCCCCCHHHHHHEEECCHHHHHHHCHHHHCHHHHHCCCCCHH
ATLAKARANLELAERNAARYRNLADRNLVSRQEAEQYETAWRLAREEEARSSAALEAARM
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESAAGRAARGVARSRLDDTVIRAPRDGIIVSRDLEKGAVVSPGQSIFTLADPAVVWVRAH
HHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEECCCCCEEEECCCEEEEEEEC
VDESQLAGVAVGREAAISLRSAPERAMQGRVARVGMESDRVTEELAVDVAFAAPPPAFRL
CCHHHHHHEEECHHHHHHHHCCHHHHHCCHHHCCCCCHHHHHHHHHEEEEECCCCCCCCC
GEQSDVLITVGTKRGVPSLPAAALTSHGGKHGVWTVSEGRLRFKPVQVGIEDRQGVTEIL
CCCCCEEEEECCCCCCCCCCHHHHHCCCCCCCEEEEECCCEEEEEEEECCCCCCCHHHHH
SGLAGAEPVVLPTPAQAKKFRDGMKVRVSP
HHHCCCCCEEECCCHHHHHHHCCCEEEECC
>Mature Secondary Structure 
TLIHTLRRHKTALVRTALLAALVVLVKATILAPPKVTTVRVERRDLTARVYGNGTVEAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEECCCCEEHH
EVVGVSSKVTGRIVSLHADQGDRVRRGQILARLESDEYAAQLGQAEAGVSRAAAGQSLEA
HHHCCCCCCCCEEEEEECCCCCCCHHHHHHEEECCHHHHHHHCHHHHCHHHHHCCCCCHH
ATLAKARANLELAERNAARYRNLADRNLVSRQEAEQYETAWRLAREEEARSSAALEAARM
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESAAGRAARGVARSRLDDTVIRAPRDGIIVSRDLEKGAVVSPGQSIFTLADPAVVWVRAH
HHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEECCCCCEEEECCCEEEEEEEC
VDESQLAGVAVGREAAISLRSAPERAMQGRVARVGMESDRVTEELAVDVAFAAPPPAFRL
CCHHHHHHEEECHHHHHHHHCCHHHHHCCHHHCCCCCHHHHHHHHHEEEEECCCCCCCCC
GEQSDVLITVGTKRGVPSLPAAALTSHGGKHGVWTVSEGRLRFKPVQVGIEDRQGVTEIL
CCCCCEEEEECCCCCCCCCCHHHHHCCCCCCCEEEEECCCEEEEEEEECCCCCCCHHHHH
SGLAGAEPVVLPTPAQAKKFRDGMKVRVSP
HHHCCCCCEEECCCHHHHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]