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The map label for this gene is kinE [H]

Identifier: 39995881

GI number: 39995881

Start: 831246

End: 833270

Strand: Reverse

Name: kinE [H]

Synonym: GSU0775

Alternate gene names: 39995881

Gene position: 833270-831246 (Counterclockwise)

Preceding gene: 39995882

Following gene: 39995875

Centisome position: 21.85

GC content: 66.57

Gene sequence:

>2025_bases
ATGAAGCCACTCCGCTTCCCCATACGTCTCAAGCTGACCTTTGCGACCCTGATCCCCCTCTTCGTCGCCACCAGCATCTG
CTGGCTCATCGGCGTCTACATCATCAACTCCCGCATCGTGGCCCAGGCCCAGGAAAAGGTCCGCACCGACCTGAACGCGG
CCCGGGCGGTCTATGACGGCGAAGTGGAGCATATCCGCGACGTGGTGAAGTTTACCGCCACCAACCCCTTCACCGCCGCG
ATCCTGGAAAAAAGCGACCGGGACACCCTCCGCAGCCTCCTGGCTCCCCTGCTCAAGGCCGAAGGGCTCGACATCCTCAC
CGCCGTGGACGCGGGGGGCAAGGTCATCTTCCGGGCCAGCAACGCCGAGGCCCACGGCGACCTGAAGTCGTCCGACGCCG
TGGTCTCCCGTGCCCTGCACGGCGAACTGGTGAGCGGCACCGACGTGCTCACCCCCGAGGAACTGGTCAAGGAGGGCACC
GCCCTGGCTGCCCGGGCCGCCATCGAGATCGTCCCCACCCCCCGAGCCCGCGCGCGGAAGGATGAGCTCGTGGAGCGCAC
CGGCATGGTCATGGTGGCCGCCTCGCCGGTGCGCGACGAGCGGGGCCGGATCGTGGGGGCGCTCTACGGCGGGGTGCTCC
TCAACGGCAATAATGAGTTGGTGGACCGGATCAAAAAGATCGTCTTCGAGGCAGTCCGCTTCCAGGGGAGAGACGTGGGG
ACCGCCACCATCTTTTTAGGCGACCTGCGCATCGCCACCAACGTCTATACCGCGCCGGACCGCCGCGCCATCGGCACCCG
GCTGTCGGAAGAGGTCTTCAACCGGGTGATCCTGAGCCGGGAGAAGTGGGTGGGACGGGCCTTCGTGGTCAACGACTGGT
ACTTCTCCTCCTACGAGCCGATCCTCAGCCCAGGGGGGGTCCCTATCGGCTCCCTCTACGTGGGGATGCCCGAGAAGCCC
TACAGCGAGATGAAGCGGGAGATAGCGCTCATCTTCGGCGTGGTGCTCTTCTGCGGCTCATTCATCGGGCTTGCCATCTC
CAGCTTCATCGCCTCGCGGCTCGCCCGCCCCATCGGCGAGCTGGAAACCGTGGCCCGGCGCATCACCGCCGGCGAACGCG
ACCTGGAGATCGCCATCCATACCCGCGACGAGATCGGCGACCTGGCCGGCGAGTTCGCCCAGATGACCCGGACTCTTGCC
CAGCGGGAGGAGGATATCCGCAAGCTGAACCGGGAGCTGGAGCAGAAGGTCCTGGACCGCACCGCCCAGCTGGAGGAAAA
GAACCTGCTCCTGGTCAAGACCCAGGAAGACCTGGTCCGGGCGGCGAAACTGGCCGACATCGGCATGCTGGCCGCCGGCG
TGGCCCACGAGATCAACAACCCCATGGCCATCATCCGGGGGAATGCGGAACTCCTTCAGATGGCGCTGCCCGACGAAGAC
CCCAACCGGGAGGAAGTGGACACCATCGCCCAGCAGGTGGGGCGGGTGGAGCGGATCATCGGCAGCCTCCTGAAATTCGC
CCGCCAGCAGCAGCGCCGGCTCGGCACGGTCTCGCTCCCCCCCCTGCTGGACGACATCCTCAAGCAGATGGGTCACCAGG
TGCCGCTTACCCGCATCGAGATCAGGAAAGCGTACGCCGTCGACCTGCCGCCCCTGGCAGGGGACCCGGACCAGCTCCGG
CAGGTCTTCACCAACCTGGTGCTCAACGCCATCCAGGCCATGCCGGACGGGGGCAGCCTCACCGTGGCCACGGCCCGGGA
CGAGGAGGCCGGCACCTGCACGGTTTCCGTGAGCGACACCGGCAGAGGCATCGCCCCAGAAAACCTGAAGAGGATCTTCA
GCCCCTTCTTCACCACCAAGAGCGAAGGGACCGGGCTGGGCCTCTCCGTCTCCTATGGCATCGTCAAGGATCACGGGGGG
AACATACTGGTGGAAAGCACGGAGGGAGAGGGAACCACCTTCCGGGTGGTGCTGCCGCTGCAACAGGGGGGGACGGAACG
GTCCGAGGGCGCACCGGGGGCGTGA

Upstream 100 bases:

>100_bases
GCCGGTCCGCCGAGATCCTGGGCATCTCGCGCAAGACCCTCGACCGCAAGATCGTCGAGTACGCCCTGACCCTCCCGGGA
GGCGTCCCGGCCGACGACGA

Downstream 100 bases:

>100_bases
CCCGACGCGGGCAGAGAGGTGCTTCAGGGAAAACAGGCAGGGGATGGAGACGGATCAGGGTGATCCAGCAGGTTTTTTCC
CGCGCGGCACGCCGGCTTCG

Product: sensor histidine kinase

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 674; Mature: 674

Protein sequence:

>674_residues
MKPLRFPIRLKLTFATLIPLFVATSICWLIGVYIINSRIVAQAQEKVRTDLNAARAVYDGEVEHIRDVVKFTATNPFTAA
ILEKSDRDTLRSLLAPLLKAEGLDILTAVDAGGKVIFRASNAEAHGDLKSSDAVVSRALHGELVSGTDVLTPEELVKEGT
ALAARAAIEIVPTPRARARKDELVERTGMVMVAASPVRDERGRIVGALYGGVLLNGNNELVDRIKKIVFEAVRFQGRDVG
TATIFLGDLRIATNVYTAPDRRAIGTRLSEEVFNRVILSREKWVGRAFVVNDWYFSSYEPILSPGGVPIGSLYVGMPEKP
YSEMKREIALIFGVVLFCGSFIGLAISSFIASRLARPIGELETVARRITAGERDLEIAIHTRDEIGDLAGEFAQMTRTLA
QREEDIRKLNRELEQKVLDRTAQLEEKNLLLVKTQEDLVRAAKLADIGMLAAGVAHEINNPMAIIRGNAELLQMALPDED
PNREEVDTIAQQVGRVERIIGSLLKFARQQQRRLGTVSLPPLLDDILKQMGHQVPLTRIEIRKAYAVDLPPLAGDPDQLR
QVFTNLVLNAIQAMPDGGSLTVATARDEEAGTCTVSVSDTGRGIAPENLKRIFSPFFTTKSEGTGLGLSVSYGIVKDHGG
NILVESTEGEGTTFRVVLPLQQGGTERSEGAPGA

Sequences:

>Translated_674_residues
MKPLRFPIRLKLTFATLIPLFVATSICWLIGVYIINSRIVAQAQEKVRTDLNAARAVYDGEVEHIRDVVKFTATNPFTAA
ILEKSDRDTLRSLLAPLLKAEGLDILTAVDAGGKVIFRASNAEAHGDLKSSDAVVSRALHGELVSGTDVLTPEELVKEGT
ALAARAAIEIVPTPRARARKDELVERTGMVMVAASPVRDERGRIVGALYGGVLLNGNNELVDRIKKIVFEAVRFQGRDVG
TATIFLGDLRIATNVYTAPDRRAIGTRLSEEVFNRVILSREKWVGRAFVVNDWYFSSYEPILSPGGVPIGSLYVGMPEKP
YSEMKREIALIFGVVLFCGSFIGLAISSFIASRLARPIGELETVARRITAGERDLEIAIHTRDEIGDLAGEFAQMTRTLA
QREEDIRKLNRELEQKVLDRTAQLEEKNLLLVKTQEDLVRAAKLADIGMLAAGVAHEINNPMAIIRGNAELLQMALPDED
PNREEVDTIAQQVGRVERIIGSLLKFARQQQRRLGTVSLPPLLDDILKQMGHQVPLTRIEIRKAYAVDLPPLAGDPDQLR
QVFTNLVLNAIQAMPDGGSLTVATARDEEAGTCTVSVSDTGRGIAPENLKRIFSPFFTTKSEGTGLGLSVSYGIVKDHGG
NILVESTEGEGTTFRVVLPLQQGGTERSEGAPGA
>Mature_674_residues
MKPLRFPIRLKLTFATLIPLFVATSICWLIGVYIINSRIVAQAQEKVRTDLNAARAVYDGEVEHIRDVVKFTATNPFTAA
ILEKSDRDTLRSLLAPLLKAEGLDILTAVDAGGKVIFRASNAEAHGDLKSSDAVVSRALHGELVSGTDVLTPEELVKEGT
ALAARAAIEIVPTPRARARKDELVERTGMVMVAASPVRDERGRIVGALYGGVLLNGNNELVDRIKKIVFEAVRFQGRDVG
TATIFLGDLRIATNVYTAPDRRAIGTRLSEEVFNRVILSREKWVGRAFVVNDWYFSSYEPILSPGGVPIGSLYVGMPEKP
YSEMKREIALIFGVVLFCGSFIGLAISSFIASRLARPIGELETVARRITAGERDLEIAIHTRDEIGDLAGEFAQMTRTLA
QREEDIRKLNRELEQKVLDRTAQLEEKNLLLVKTQEDLVRAAKLADIGMLAAGVAHEINNPMAIIRGNAELLQMALPDED
PNREEVDTIAQQVGRVERIIGSLLKFARQQQRRLGTVSLPPLLDDILKQMGHQVPLTRIEIRKAYAVDLPPLAGDPDQLR
QVFTNLVLNAIQAMPDGGSLTVATARDEEAGTCTVSVSDTGRGIAPENLKRIFSPFFTTKSEGTGLGLSVSYGIVKDHGG
NILVESTEGEGTTFRVVLPLQQGGTERSEGAPGA

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=226, Percent_Identity=40.7079646017699, Blast_Score=165, Evalue=9e-42,
Organism=Escherichia coli, GI1788549, Length=235, Percent_Identity=34.0425531914894, Blast_Score=142, Evalue=1e-34,
Organism=Escherichia coli, GI1790300, Length=245, Percent_Identity=34.2857142857143, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI87081816, Length=348, Percent_Identity=29.3103448275862, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1786600, Length=288, Percent_Identity=28.4722222222222, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1789149, Length=350, Percent_Identity=27.4285714285714, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1788393, Length=325, Percent_Identity=23.6923076923077, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI145693157, Length=263, Percent_Identity=28.8973384030418, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790346, Length=224, Percent_Identity=27.6785714285714, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1790861, Length=210, Percent_Identity=28.5714285714286, Blast_Score=73, Evalue=7e-14,
Organism=Escherichia coli, GI1786912, Length=229, Percent_Identity=28.3842794759825, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI1786783, Length=261, Percent_Identity=27.2030651340996, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI87082128, Length=219, Percent_Identity=26.9406392694064, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI1787894, Length=236, Percent_Identity=27.1186440677966, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI48994928, Length=241, Percent_Identity=26.5560165975104, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1788713, Length=228, Percent_Identity=25.8771929824561, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1789808, Length=221, Percent_Identity=25.7918552036199, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 73534; Mature: 73534

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPLRFPIRLKLTFATLIPLFVATSICWLIGVYIINSRIVAQAQEKVRTDLNAARAVYDG
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EVEHIRDVVKFTATNPFTAAILEKSDRDTLRSLLAPLLKAEGLDILTAVDAGGKVIFRAS
CHHHHHHHHHHHCCCCCEEHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEC
NAEAHGDLKSSDAVVSRALHGELVSGTDVLTPEELVKEGTALAARAAIEIVPTPRARARK
CCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHCCHHHHHHHEEEEECCCCHHHHH
DELVERTGMVMVAASPVRDERGRIVGALYGGVLLNGNNELVDRIKKIVFEAVRFQGRDVG
HHHHHHCCEEEEEECCCCCCCCCEEEEEECCEEECCCHHHHHHHHHHHHHHHHHCCCCCC
TATIFLGDLRIATNVYTAPDRRAIGTRLSEEVFNRVILSREKWVGRAFVVNDWYFSSYEP
EEEEEEECEEEEECEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCC
ILSPGGVPIGSLYVGMPEKPYSEMKREIALIFGVVLFCGSFIGLAISSFIASRLARPIGE
CCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LETVARRITAGERDLEIAIHTRDEIGDLAGEFAQMTRTLAQREEDIRKLNRELEQKVLDR
HHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TAQLEEKNLLLVKTQEDLVRAAKLADIGMLAAGVAHEINNPMAIIRGNAELLQMALPDED
HHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHEECCCCC
PNREEVDTIAQQVGRVERIIGSLLKFARQQQRRLGTVSLPPLLDDILKQMGHQVPLTRIE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHEE
IRKAYAVDLPPLAGDPDQLRQVFTNLVLNAIQAMPDGGSLTVATARDEEAGTCTVSVSDT
EHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEECCC
GRGIAPENLKRIFSPFFTTKSEGTGLGLSVSYGIVKDHGGNILVESTEGEGTTFRVVLPL
CCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECEEEECCCEEEEECCCCCCCEEEEEEEE
QQGGTERSEGAPGA
CCCCCCCCCCCCCC
>Mature Secondary Structure
MKPLRFPIRLKLTFATLIPLFVATSICWLIGVYIINSRIVAQAQEKVRTDLNAARAVYDG
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EVEHIRDVVKFTATNPFTAAILEKSDRDTLRSLLAPLLKAEGLDILTAVDAGGKVIFRAS
CHHHHHHHHHHHCCCCCEEHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEC
NAEAHGDLKSSDAVVSRALHGELVSGTDVLTPEELVKEGTALAARAAIEIVPTPRARARK
CCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHCCHHHHHHHEEEEECCCCHHHHH
DELVERTGMVMVAASPVRDERGRIVGALYGGVLLNGNNELVDRIKKIVFEAVRFQGRDVG
HHHHHHCCEEEEEECCCCCCCCCEEEEEECCEEECCCHHHHHHHHHHHHHHHHHCCCCCC
TATIFLGDLRIATNVYTAPDRRAIGTRLSEEVFNRVILSREKWVGRAFVVNDWYFSSYEP
EEEEEEECEEEEECEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCC
ILSPGGVPIGSLYVGMPEKPYSEMKREIALIFGVVLFCGSFIGLAISSFIASRLARPIGE
CCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LETVARRITAGERDLEIAIHTRDEIGDLAGEFAQMTRTLAQREEDIRKLNRELEQKVLDR
HHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TAQLEEKNLLLVKTQEDLVRAAKLADIGMLAAGVAHEINNPMAIIRGNAELLQMALPDED
HHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHEECCCCC
PNREEVDTIAQQVGRVERIIGSLLKFARQQQRRLGTVSLPPLLDDILKQMGHQVPLTRIE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHEE
IRKAYAVDLPPLAGDPDQLRQVFTNLVLNAIQAMPDGGSLTVATARDEEAGTCTVSVSDT
EHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEECCC
GRGIAPENLKRIFSPFFTTKSEGTGLGLSVSYGIVKDHGGNILVESTEGEGTTFRVVLPL
CCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECEEEECCCEEEEECCCCCCCEEEEEEEE
QQGGTERSEGAPGA
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]