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The map label for this gene is ispE

Identifier: 39995766

GI number: 39995766

Start: 696965

End: 697804

Strand: Direct

Name: ispE

Synonym: GSU0660

Alternate gene names: 39995766

Gene position: 696965-697804 (Clockwise)

Preceding gene: 39995765

Following gene: 39995767

Centisome position: 18.27

GC content: 62.38

Gene sequence:

>840_bases
GTGAAGAAGCTGACGCTCAAGGCCCCGGCCAAGGTCAATTACCGCCTCGACGTTCTGAGGCGGCGCCCCGACGGCTACCA
TGATTTGCGGATGATCATGCAGCGGATCGATCTCTGTGACGAGATAGAGATCTGCCTCACGGACGGGCCCGGCATTCGGG
TGGTCTGCGGCCGCGAGGGGGTGCCTGATGGGCCCGGCAATATTGCCTGGCGCGCTGCTGACGCCCTGCTCGCCCTGTCC
GCCGACAAGCCTGGTATTGATATCTCCATAACCAAGAAGATTCCGGTGGCGGCCGGCCTGGGAGGCGGAAGCAGTGACGC
GGCGACGGTGCTCATGGGGGTGAACGAGCTGCTGGGACTGGATCTGCCCGAAAAGCGTCTCCGGGAGATCGGCGTCACCC
TCGGCGCTGACGTGCCGTTTTTCATCTTCGGCCGGACAGCCCTGGCCGAAGGGATCGGCGAGGAGCTGACCGCCATCGAC
CGAGTGCCCGCCGCCTGGATTGTGGTGGTCAATCCCAATGTTCCCGTTTCGACGGCATGGGTCTATCAAAATTTGCAATT
GACAGGTGAGGCCGCCAGGGTTAAAATTCCCCGTTTCTTCGAGAGTGTCGCGGAGGTCTGCGCGATTCTCTCCAATGACC
TTGAATCGGTCACTATCCCGCGGTATCCGGTTATCGGCGAGATCAAGCGTGAGCTTCTCGCCGCCGGGGCGCTCGGTTCC
CTCATGTCGGGAAGCGGGCCGACGGTTTTTGCACTCTTCGAGGAAGAGGACGCAGCCGTTCGAGCCGCCGAGATGATGCG
CGCGCGGAGTTGGTTCGCCGCAGCGGTCAGGACAATCTGA

Upstream 100 bases:

>100_bases
TCTCTCGGAGGGGATGGGTGACTGAGGTGATTTCCCCTTGCGTTACCCGGGCTTTTGCTTTACAAAAAAATCTTTCCATT
TTTCCCCAGGTGGATGCGAC

Downstream 100 bases:

>100_bases
GCGGAGCCGTGCCGGCTATGCGGAAGCCCGGGCAGGCACGGCACATACGGCACGTTGGGGTGTCGCCAAGCGGTAAGGCA
CCGGATTTTGATTCCGGCAT

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MKKLTLKAPAKVNYRLDVLRRRPDGYHDLRMIMQRIDLCDEIEICLTDGPGIRVVCGREGVPDGPGNIAWRAADALLALS
ADKPGIDISITKKIPVAAGLGGGSSDAATVLMGVNELLGLDLPEKRLREIGVTLGADVPFFIFGRTALAEGIGEELTAID
RVPAAWIVVVNPNVPVSTAWVYQNLQLTGEAARVKIPRFFESVAEVCAILSNDLESVTIPRYPVIGEIKRELLAAGALGS
LMSGSGPTVFALFEEEDAAVRAAEMMRARSWFAAAVRTI

Sequences:

>Translated_279_residues
MKKLTLKAPAKVNYRLDVLRRRPDGYHDLRMIMQRIDLCDEIEICLTDGPGIRVVCGREGVPDGPGNIAWRAADALLALS
ADKPGIDISITKKIPVAAGLGGGSSDAATVLMGVNELLGLDLPEKRLREIGVTLGADVPFFIFGRTALAEGIGEELTAID
RVPAAWIVVVNPNVPVSTAWVYQNLQLTGEAARVKIPRFFESVAEVCAILSNDLESVTIPRYPVIGEIKRELLAAGALGS
LMSGSGPTVFALFEEEDAAVRAAEMMRARSWFAAAVRTI
>Mature_279_residues
MKKLTLKAPAKVNYRLDVLRRRPDGYHDLRMIMQRIDLCDEIEICLTDGPGIRVVCGREGVPDGPGNIAWRAADALLALS
ADKPGIDISITKKIPVAAGLGGGSSDAATVLMGVNELLGLDLPEKRLREIGVTLGADVPFFIFGRTALAEGIGEELTAID
RVPAAWIVVVNPNVPVSTAWVYQNLQLTGEAARVKIPRFFESVAEVCAILSNDLESVTIPRYPVIGEIKRELLAAGALGS
LMSGSGPTVFALFEEEDAAVRAAEMMRARSWFAAAVRTI

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=275, Percent_Identity=36.7272727272727, Blast_Score=134, Evalue=7e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_GEOSL (Q74FE9)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_951717.1
- ProteinModelPortal:   Q74FE9
- SMR:   Q74FE9
- GeneID:   2685508
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU0660
- NMPDR:   fig|243231.1.peg.655
- TIGR:   GSU0660
- HOGENOM:   HBG734593
- OMA:   LNLFLHV
- ProtClustDB:   PRK14614
- BioCyc:   GSUL243231:GSU_0660-MONOMER
- BRENDA:   2.7.1.148
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 29963; Mature: 29963

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 137-137

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLTLKAPAKVNYRLDVLRRRPDGYHDLRMIMQRIDLCDEIEICLTDGPGIRVVCGREG
CCCEEECCCCCCCEEEEEHHCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCC
VPDGPGNIAWRAADALLALSADKPGIDISITKKIPVAAGLGGGSSDAATVLMGVNELLGL
CCCCCCCEEEEECCEEEEEECCCCCCEEEEECCCCEEECCCCCCCCHHHHHHHHHHHHCC
DLPEKRLREIGVTLGADVPFFIFGRTALAEGIGEELTAIDRVPAAWIVVVNPNVPVSTAW
CCCHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEE
VYQNLQLTGEAARVKIPRFFESVAEVCAILSNDLESVTIPRYPVIGEIKRELLAAGALGS
EEECEEEECCHHEEECHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHH
LMSGSGPTVFALFEEEDAAVRAAEMMRARSWFAAAVRTI
HHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKLTLKAPAKVNYRLDVLRRRPDGYHDLRMIMQRIDLCDEIEICLTDGPGIRVVCGREG
CCCEEECCCCCCCEEEEEHHCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCC
VPDGPGNIAWRAADALLALSADKPGIDISITKKIPVAAGLGGGSSDAATVLMGVNELLGL
CCCCCCCEEEEECCEEEEEECCCCCCEEEEECCCCEEECCCCCCCCHHHHHHHHHHHHCC
DLPEKRLREIGVTLGADVPFFIFGRTALAEGIGEELTAIDRVPAAWIVVVNPNVPVSTAW
CCCHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEE
VYQNLQLTGEAARVKIPRFFESVAEVCAILSNDLESVTIPRYPVIGEIKRELLAAGALGS
EEECEEEECCHHEEECHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHH
LMSGSGPTVFALFEEEDAAVRAAEMMRARSWFAAAVRTI
HHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA