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The map label for this gene is cofH [H]

Identifier: 39995549

GI number: 39995549

Start: 472705

End: 473790

Strand: Direct

Name: cofH [H]

Synonym: GSU0441

Alternate gene names: 39995549

Gene position: 472705-473790 (Clockwise)

Preceding gene: 39995548

Following gene: 39995550

Centisome position: 12.39

GC content: 65.19

Gene sequence:

>1086_bases
ATGAGTATTTCATTGGCGACAATTAGCGGCCGGGTCCATGCCGGCGAACGGATCAGCGACGAGGAGGCCCTGTTCCTCTT
CGAGAGCCGCGACCCCCTGGCCGTGGGAGAACTGGCCGCAGCCGTCAACCGCCGCCGCAACGGCGACCGGGTCTTCTTCA
ACGTGAACCGGCACATCAACCACACGAACATCTGCGTCAACCGCTGCTCCTTCTGTGCCTTCTACCGCGCCGCCGACGAG
CCGGGGGCATACCTCTACGACCTGGAGGAGATCCGCAACCGCGCGGCCGAGGCCCACGCCCAGGGTGCCACCGAGATTCA
CATCGTGGGCGGCCTCCACCCCGATCTTCCCTTCGACTTCTATCTCGCCATGCTCCGGACCGTGAAGGAGGTCTCCCCGG
ACCTCCACGTGAAGGCCTTTACCGCGGTTGAGATCGAGTACCTGTCGCGGCTCGCCGGCCTTTCGACGGCCGAAACCCTG
ACAGTGCTGAAGGAGGCGGGACTCGGCTCGCTCCCCGGCGGCGGGGCGGAAATCTTTGCTCCGGCCGTGCGCAACCGGCT
CTGTCCCGAGAAGATCTCCGGCGACAAGTGGCTGGCCATCATGGAGGAGGTCCACCGGGCCGGGCTCAAATCCAATGCCA
CCATGCTCTACGGCCACATCGAGAGCTACGCGGACCGCGTGGACCACATGCGCCGCCTGCGTGAGCTTCAGGACCGCACC
GGCGGCTTCCAGGTCTTCATCCCCCTGGCCTTCCAGAAGGATAACAACCCCCTGGGGCACCTGAAACGCCCCGGGCCCGG
TGGGGTCGACGCCCTGCTCACCCTGGCCGTGGCCCGCATCTACCTGGACAATTTCGCCAATATCAAAGCCTACTGGGTCA
TGCTCGGGGTAAAGATCGCCCAGACTTCCCTGGCCTTCGGGGTAAACGATCTGGACGGCACGGTGGTGGAGGAGAAGATC
GGCCATGATGCCGGCGCCGCTTCCCCCCAGACCATGGGGCGCGACGAAATCGTCTCCCTGATCCGCACGGCCGGCCGGGT
GCCGGTAGAGCGGGATACGCTGTACAACGAACTGCGGGTGTACTGA

Upstream 100 bases:

>100_bases
CTGGTCGGTTTTGTGGTGGGCAAGGTGCTGGACGCACTTCAGATTCCCCATGAGTTGTTCACCCGCTGGGGTGACGTATC
CCGGGTGGAGGAGTGATGGG

Downstream 100 bases:

>100_bases
TGCCGATACGTGCCATGGAAAAAGCAACCGCCGGGAAACTCCTCACGCGGGCCGAAGCGCTCAGCCTCCTGACGGAGGGG
GACCTCCTCGCCCTGGGCCG

Product: radical SAM protein

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2 [H]

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MSISLATISGRVHAGERISDEEALFLFESRDPLAVGELAAAVNRRRNGDRVFFNVNRHINHTNICVNRCSFCAFYRAADE
PGAYLYDLEEIRNRAAEAHAQGATEIHIVGGLHPDLPFDFYLAMLRTVKEVSPDLHVKAFTAVEIEYLSRLAGLSTAETL
TVLKEAGLGSLPGGGAEIFAPAVRNRLCPEKISGDKWLAIMEEVHRAGLKSNATMLYGHIESYADRVDHMRRLRELQDRT
GGFQVFIPLAFQKDNNPLGHLKRPGPGGVDALLTLAVARIYLDNFANIKAYWVMLGVKIAQTSLAFGVNDLDGTVVEEKI
GHDAGAASPQTMGRDEIVSLIRTAGRVPVERDTLYNELRVY

Sequences:

>Translated_361_residues
MSISLATISGRVHAGERISDEEALFLFESRDPLAVGELAAAVNRRRNGDRVFFNVNRHINHTNICVNRCSFCAFYRAADE
PGAYLYDLEEIRNRAAEAHAQGATEIHIVGGLHPDLPFDFYLAMLRTVKEVSPDLHVKAFTAVEIEYLSRLAGLSTAETL
TVLKEAGLGSLPGGGAEIFAPAVRNRLCPEKISGDKWLAIMEEVHRAGLKSNATMLYGHIESYADRVDHMRRLRELQDRT
GGFQVFIPLAFQKDNNPLGHLKRPGPGGVDALLTLAVARIYLDNFANIKAYWVMLGVKIAQTSLAFGVNDLDGTVVEEKI
GHDAGAASPQTMGRDEIVSLIRTAGRVPVERDTLYNELRVY
>Mature_360_residues
SISLATISGRVHAGERISDEEALFLFESRDPLAVGELAAAVNRRRNGDRVFFNVNRHINHTNICVNRCSFCAFYRAADEP
GAYLYDLEEIRNRAAEAHAQGATEIHIVGGLHPDLPFDFYLAMLRTVKEVSPDLHVKAFTAVEIEYLSRLAGLSTAETLT
VLKEAGLGSLPGGGAEIFAPAVRNRLCPEKISGDKWLAIMEEVHRAGLKSNATMLYGHIESYADRVDHMRRLRELQDRTG
GFQVFIPLAFQKDNNPLGHLKRPGPGGVDALLTLAVARIYLDNFANIKAYWVMLGVKIAQTSLAFGVNDLDGTVVEEKIG
HDAGAASPQTMGRDEIVSLIRTAGRVPVERDTLYNELRVY

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 39799; Mature: 39668

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISLATISGRVHAGERISDEEALFLFESRDPLAVGELAAAVNRRRNGDRVFFNVNRHIN
CCEEEEEECCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCC
HTNICVNRCSFCAFYRAADEPGAYLYDLEEIRNRAAEAHAQGATEIHIVGGLHPDLPFDF
HHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHH
YLAMLRTVKEVSPDLHVKAFTAVEIEYLSRLAGLSTAETLTVLKEAGLGSLPGGGAEIFA
HHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHH
PAVRNRLCPEKISGDKWLAIMEEVHRAGLKSNATMLYGHIESYADRVDHMRRLRELQDRT
HHHHCCCCCHHCCCCHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCC
GGFQVFIPLAFQKDNNPLGHLKRPGPGGVDALLTLAVARIYLDNFANIKAYWVMLGVKIA
CCEEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
QTSLAFGVNDLDGTVVEEKIGHDAGAASPQTMGRDEIVSLIRTAGRVPVERDTLYNELRV
HHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHC
Y
C
>Mature Secondary Structure 
SISLATISGRVHAGERISDEEALFLFESRDPLAVGELAAAVNRRRNGDRVFFNVNRHIN
CEEEEEECCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCC
HTNICVNRCSFCAFYRAADEPGAYLYDLEEIRNRAAEAHAQGATEIHIVGGLHPDLPFDF
HHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHH
YLAMLRTVKEVSPDLHVKAFTAVEIEYLSRLAGLSTAETLTVLKEAGLGSLPGGGAEIFA
HHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHH
PAVRNRLCPEKISGDKWLAIMEEVHRAGLKSNATMLYGHIESYADRVDHMRRLRELQDRT
HHHHCCCCCHHCCCCHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCC
GGFQVFIPLAFQKDNNPLGHLKRPGPGGVDALLTLAVARIYLDNFANIKAYWVMLGVKIA
CCEEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
QTSLAFGVNDLDGTVVEEKIGHDAGAASPQTMGRDEIVSLIRTAGRVPVERDTLYNELRV
HHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHC
Y
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]