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The map label for this gene is nuoM-1 [H]

Identifier: 39995459

GI number: 39995459

Start: 379791

End: 381359

Strand: Direct

Name: nuoM-1 [H]

Synonym: GSU0350

Alternate gene names: 39995459

Gene position: 379791-381359 (Clockwise)

Preceding gene: 39995458

Following gene: 39995460

Centisome position: 9.96

GC content: 51.43

Gene sequence:

>1569_bases
ATGAGCCAGTTTCCGTTAATCAGCGTGATGACCTTTCTTCCACTCCTGGGGGTCGTACTGCTCTTCTTCGTCCCCAAGCA
GAGCCACTCGGTGCACAGGATGCTCGCCTTGCTGGTCTCTCTGGTGACCTTTGTCGTGTCGATACCTCTGGTTACTGGAT
TCCAGAGTAACGCCGACTTCCAGTTCGTGGAGAAAATGCCGTGGATAGCTGCGGGTCCGTTCCAGATGAACTATCACGTT
GCAATCGATGGTATCAGTCTCTGGCTCGTTCTCCTCACGACATTTATCATGCCGATCGCCATCCTCTCTACCTGGACAGC
GGTAGAAGAGAAGGTCAAGGAGTACATGATCTGCCTCTTGCTGCTTGAAGTGGGAATGCTTGGAGCTTTCGTGTCGCTTG
ATCTCTTCCTGTTCTACATCTTCTGGGAAGTGATGCTCATACCGATGTATTTCATCATCGGGATCTGGGGTGGTAAAAAT
AAGATTTATGCTGCAGTCAAATTCTTCATCTACACGATGGTTGGCTCCATGCTCATGCTGGTCGCTCTGGTTACCCTCTA
CTTCAAGGCAGGGGCTGGAGACTTCAACGTGCTCAGGTTCTATGAAGTAACTCTTGATCCGGCAACACAAGTCTGGATGT
TCTTGGCCTTTGCCCTGGCATTTGCCATCAAAGTGCCTATGTTCCCTCTCCACACGTGGCTTCCGGATGCTCACACCGAA
GCGCCGACGGCCGGCTCGGTCATTCTGGCTGCAGTGCTCCTGAAGATGGGGACTTACGGTTTCGTTCGTTTTGCAATGCC
GCTCTTCCCCGACGCAACCGATAAATTCACCCCCCTCATTGCTACGCTTTCGGTTATTGGCATCATATACGCTGCCCTTG
TTGCAATGGTCCAAGAAGATGTCAAGAAGCTTGTAGCGTACTCATCTGTTGCTCACCTGGGCTACGTTATGCTGGGTGTG
TTCGCACTGAATCTTCAAGGACTTTCCGGTGGAATGCTGCAAATGCTCAACCACGGTGTCTCAACCGGCGCACTGTTCCT
CATCGTTGGCTTTATTTATGAGCGCCGCCACACTCGACTGATCACGGATTTCGGAGGGCTCGCCAAGCAAATGCCGGTGT
TCGCTACGATATTTATGATCGTCACGCTTTCCTCGATCGGTCTACCCGGCACGAACGGTTTCGTAGGGGAATTCCTTGTT
CTTCTCGGTGCGTTTGAAAGTGAGCTTCGTTGGTATGCTGTAATCGGTACAAGTGGTGTTATTCTGGCAGCCGTTTACAT
GCTCTGGATGTTCCAGCGCGTTATGTTCGGGGAGCTCACAAACCCAAAGAACCAGACCTTGTGTGATCTGAATGCACGTG
AAGTCGGGATCATGCTTCCGCTACTCGTTCTGATCTTCGTGATGGGTGTATATCCCACTCCTTTCATTGAGAAGATGAGT
CCTTCACTTGAGAAACTCATTGTTCATGTGAAGACCAAGCAGCAGGGTGCCGTTGCTCAGGTGGAGACCGTGCCTGCTGC
TCCCCAGGTCATGCCTGCAGCTGCTGTTGTGCCTGCAGAACATAAGTAA

Upstream 100 bases:

>100_bases
GCACAACTATGCCTTCTCCATGGCTGTTGGCGTGGTGGTGATCGTAGCGTTTTACCTCTTCCGCTAACAAAGTATTACTA
GGACTAAAGGAGCAGAGTTC

Downstream 100 bases:

>100_bases
ATCCGTCTCAGAATAGCCACCGGAGGGATATTAGATGGAAACAATCGTACTCCCAGTCATTAACCTCGCACCCATCATGC
CCGAGATCATACTTTCGGTC

Product: NADH dehydrogenase I subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M [H]

Number of amino acids: Translated: 522; Mature: 521

Protein sequence:

>522_residues
MSQFPLISVMTFLPLLGVVLLFFVPKQSHSVHRMLALLVSLVTFVVSIPLVTGFQSNADFQFVEKMPWIAAGPFQMNYHV
AIDGISLWLVLLTTFIMPIAILSTWTAVEEKVKEYMICLLLLEVGMLGAFVSLDLFLFYIFWEVMLIPMYFIIGIWGGKN
KIYAAVKFFIYTMVGSMLMLVALVTLYFKAGAGDFNVLRFYEVTLDPATQVWMFLAFALAFAIKVPMFPLHTWLPDAHTE
APTAGSVILAAVLLKMGTYGFVRFAMPLFPDATDKFTPLIATLSVIGIIYAALVAMVQEDVKKLVAYSSVAHLGYVMLGV
FALNLQGLSGGMLQMLNHGVSTGALFLIVGFIYERRHTRLITDFGGLAKQMPVFATIFMIVTLSSIGLPGTNGFVGEFLV
LLGAFESELRWYAVIGTSGVILAAVYMLWMFQRVMFGELTNPKNQTLCDLNAREVGIMLPLLVLIFVMGVYPTPFIEKMS
PSLEKLIVHVKTKQQGAVAQVETVPAAPQVMPAAAVVPAEHK

Sequences:

>Translated_522_residues
MSQFPLISVMTFLPLLGVVLLFFVPKQSHSVHRMLALLVSLVTFVVSIPLVTGFQSNADFQFVEKMPWIAAGPFQMNYHV
AIDGISLWLVLLTTFIMPIAILSTWTAVEEKVKEYMICLLLLEVGMLGAFVSLDLFLFYIFWEVMLIPMYFIIGIWGGKN
KIYAAVKFFIYTMVGSMLMLVALVTLYFKAGAGDFNVLRFYEVTLDPATQVWMFLAFALAFAIKVPMFPLHTWLPDAHTE
APTAGSVILAAVLLKMGTYGFVRFAMPLFPDATDKFTPLIATLSVIGIIYAALVAMVQEDVKKLVAYSSVAHLGYVMLGV
FALNLQGLSGGMLQMLNHGVSTGALFLIVGFIYERRHTRLITDFGGLAKQMPVFATIFMIVTLSSIGLPGTNGFVGEFLV
LLGAFESELRWYAVIGTSGVILAAVYMLWMFQRVMFGELTNPKNQTLCDLNAREVGIMLPLLVLIFVMGVYPTPFIEKMS
PSLEKLIVHVKTKQQGAVAQVETVPAAPQVMPAAAVVPAEHK
>Mature_521_residues
SQFPLISVMTFLPLLGVVLLFFVPKQSHSVHRMLALLVSLVTFVVSIPLVTGFQSNADFQFVEKMPWIAAGPFQMNYHVA
IDGISLWLVLLTTFIMPIAILSTWTAVEEKVKEYMICLLLLEVGMLGAFVSLDLFLFYIFWEVMLIPMYFIIGIWGGKNK
IYAAVKFFIYTMVGSMLMLVALVTLYFKAGAGDFNVLRFYEVTLDPATQVWMFLAFALAFAIKVPMFPLHTWLPDAHTEA
PTAGSVILAAVLLKMGTYGFVRFAMPLFPDATDKFTPLIATLSVIGIIYAALVAMVQEDVKKLVAYSSVAHLGYVMLGVF
ALNLQGLSGGMLQMLNHGVSTGALFLIVGFIYERRHTRLITDFGGLAKQMPVFATIFMIVTLSSIGLPGTNGFVGEFLVL
LGAFESELRWYAVIGTSGVILAAVYMLWMFQRVMFGELTNPKNQTLCDLNAREVGIMLPLLVLIFVMGVYPTPFIEKMSP
SLEKLIVHVKTKQQGAVAQVETVPAAPQVMPAAAVVPAEHK

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=371, Percent_Identity=32.8840970350404, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI251831117, Length=455, Percent_Identity=24.6153846153846, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1788613, Length=498, Percent_Identity=36.9477911646586, Blast_Score=324, Evalue=1e-89,
Organism=Escherichia coli, GI1788827, Length=286, Percent_Identity=30.7692307692308, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1788831, Length=363, Percent_Identity=28.3746556473829, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1788614, Length=503, Percent_Identity=26.0437375745527, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI145693160, Length=357, Percent_Identity=25.4901960784314, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1788829, Length=413, Percent_Identity=24.2130750605327, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI2367154, Length=337, Percent_Identity=23.4421364985163, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918
- InterPro:   IPR000260 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01059 Oxidored_q5_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 57644; Mature: 57513

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQFPLISVMTFLPLLGVVLLFFVPKQSHSVHRMLALLVSLVTFVVSIPLVTGFQSNADF
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
QFVEKMPWIAAGPFQMNYHVAIDGISLWLVLLTTFIMPIAILSTWTAVEEKVKEYMICLL
HHHHHCCCEEECCEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLEVGMLGAFVSLDLFLFYIFWEVMLIPMYFIIGIWGGKNKIYAAVKFFIYTMVGSMLML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
VALVTLYFKAGAGDFNVLRFYEVTLDPATQVWMFLAFALAFAIKVPMFPLHTWLPDAHTE
HHHHHHHHHCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCC
APTAGSVILAAVLLKMGTYGFVRFAMPLFPDATDKFTPLIATLSVIGIIYAALVAMVQED
CCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VKKLVAYSSVAHLGYVMLGVFALNLQGLSGGMLQMLNHGVSTGALFLIVGFIYERRHTRL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
ITDFGGLAKQMPVFATIFMIVTLSSIGLPGTNGFVGEFLVLLGAFESELRWYAVIGTSGV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCHH
ILAAVYMLWMFQRVMFGELTNPKNQTLCDLNAREVGIMLPLLVLIFVMGVYPTPFIEKMS
HHHHHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHC
PSLEKLIVHVKTKQQGAVAQVETVPAAPQVMPAAAVVPAEHK
CHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCHHCCCCCCC
>Mature Secondary Structure 
SQFPLISVMTFLPLLGVVLLFFVPKQSHSVHRMLALLVSLVTFVVSIPLVTGFQSNADF
CCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH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CHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8566820 [H]