Click here to switch to the map view.

The map label for this gene is nuoN-1 [H]

Identifier: 39995460

GI number: 39995460

Start: 381394

End: 382848

Strand: Direct

Name: nuoN-1 [H]

Synonym: GSU0351

Alternate gene names: 39995460

Gene position: 381394-382848 (Clockwise)

Preceding gene: 39995459

Following gene: 39995461

Centisome position: 10.0

GC content: 51.89

Gene sequence:

>1455_bases
ATGGAAACAATCGTACTCCCAGTCATTAACCTCGCACCCATCATGCCCGAGATCATACTTTCGGTCTTTGGCATGGCGCT
GCTTCTCGTGAATGTGTTTGTACCGAGTGAGCAGAAGGCCTATCTGGGATACCTGAGCCTCGTAGGCATCATCGCAGCTG
GCGTATCAATCGTCGGGGGATGGGGAGTACCACTCGACTCATTCAGCGGCTCTGTTGTGCAGGACAACTTCGCTCTCTTC
TTCAAAGGGATATTCCTGCTCTCGGCCGGGCTGACGATTCTAATCTCCGATCATTACATGAAGCGCGAAGGGTGTAACCA
GGGAGAACTCTATCCTCTGATTCTCTTCGCTACCATGGGAATGATGCTCATGGCATCCGGAACGGATCTCATGACGGTGT
TCCTCGGTCTTGAAGTACTGTCAGTTTCGTTGTATGTCCTTGCAGGATTCAATCGGGCCAATCTCAAATCGAATGAGGCA
GGGCTCAAATACTTCCTTCTCGGCGCGTTTTCGACCGGTTTCCTTCTCTATGGCATGGCATTGACCTATGGTGCTACCGG
TACCACGAAAATCCCTAAAATTGCTGAGTTTGTAGCTGCCAACGGCATGGTAGCCCAATCGCCGATGTTCTATATCGGCA
TGCTTCTCATGGCGTCTGGGTTCGCGTTCAAGATTGCAGCGGCTCCCTTCCACATGTGGACTCCTGACGTCTATGAAGGT
GCTCCGACTCCGATGACTGCCTTCATGTCCGCTGGTCCTAAAGCAGCCGGTTTTGCCGCTTTCATGAGGGTCCTGATAGT
GGCATTCCCTACCCTCAAGGCCGACTGGTCTGACCTGCTGTGGGTCCTTGCAGTACTTACCATGACAATCGGCAACCTGA
TTGCACTCAATCAGGATAATATCAAAAGGATGCTTGCCTACTCGTCCATTGCCCACGCCGGCTACGCACTTGTCGGTTTC
ACCGCCGGAAACGCTGAAGGAGCAGCCGGTATCCTGTTCTATATGCTTTCCTATGCGTTCATGAACATCGGAGCCTTTGC
AATCATCATCCTTGTGGGCAAGAAGGGTGAGGAAAATAACAATGTCGCAGACTACGCTGGTTTTGCCACAAAGCATCCGG
TCCTGGCGCTGGCCATGGCGATCTTCCTGTTTTCCTTGGCAGGCATGCCCCCTACGGCTGGATTCATCGGTAAGTTTTAT
CTCTTCTCGGGCGCTATCAAGGCCGGTTACATCTGGCTCGCCATCATCGGTGTGCTGAACAGTGCCGCCTCTGTCTACTA
CTACCTCAGAGTTATGGTTTACATGTACATGAAGAATCCGACTGAGGAATTTGATTGGATGACCCTTTCCCCGGCAGTCA
TGCTCTGCATTCTGATTGCCGTGGTAGGCGTCATCATTCCCGGTGTGGTTCCTTCGTATCTTCTCGAACTTGCCCAAAAA
GCCGTCCTGCTTTAA

Upstream 100 bases:

>100_bases
GAGACCGTGCCTGCTGCTCCCCAGGTCATGCCTGCAGCTGCTGTTGTGCCTGCAGAACATAAGTAAATCCGTCTCAGAAT
AGCCACCGGAGGGATATTAG

Downstream 100 bases:

>100_bases
TAACGGCATGAACCTTTGAAAAAAGCCCCTCCGAGGAGGGGCTTTTTTTTGTTCAAGTCTGTTAGACTTGATATAACACT
ATTATTCACAAGGAGGAGAC

Product: NADH dehydrogenase I subunit N

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
METIVLPVINLAPIMPEIILSVFGMALLLVNVFVPSEQKAYLGYLSLVGIIAAGVSIVGGWGVPLDSFSGSVVQDNFALF
FKGIFLLSAGLTILISDHYMKREGCNQGELYPLILFATMGMMLMASGTDLMTVFLGLEVLSVSLYVLAGFNRANLKSNEA
GLKYFLLGAFSTGFLLYGMALTYGATGTTKIPKIAEFVAANGMVAQSPMFYIGMLLMASGFAFKIAAAPFHMWTPDVYEG
APTPMTAFMSAGPKAAGFAAFMRVLIVAFPTLKADWSDLLWVLAVLTMTIGNLIALNQDNIKRMLAYSSIAHAGYALVGF
TAGNAEGAAGILFYMLSYAFMNIGAFAIIILVGKKGEENNNVADYAGFATKHPVLALAMAIFLFSLAGMPPTAGFIGKFY
LFSGAIKAGYIWLAIIGVLNSAASVYYYLRVMVYMYMKNPTEEFDWMTLSPAVMLCILIAVVGVIIPGVVPSYLLELAQK
AVLL

Sequences:

>Translated_484_residues
METIVLPVINLAPIMPEIILSVFGMALLLVNVFVPSEQKAYLGYLSLVGIIAAGVSIVGGWGVPLDSFSGSVVQDNFALF
FKGIFLLSAGLTILISDHYMKREGCNQGELYPLILFATMGMMLMASGTDLMTVFLGLEVLSVSLYVLAGFNRANLKSNEA
GLKYFLLGAFSTGFLLYGMALTYGATGTTKIPKIAEFVAANGMVAQSPMFYIGMLLMASGFAFKIAAAPFHMWTPDVYEG
APTPMTAFMSAGPKAAGFAAFMRVLIVAFPTLKADWSDLLWVLAVLTMTIGNLIALNQDNIKRMLAYSSIAHAGYALVGF
TAGNAEGAAGILFYMLSYAFMNIGAFAIIILVGKKGEENNNVADYAGFATKHPVLALAMAIFLFSLAGMPPTAGFIGKFY
LFSGAIKAGYIWLAIIGVLNSAASVYYYLRVMVYMYMKNPTEEFDWMTLSPAVMLCILIAVVGVIIPGVVPSYLLELAQK
AVLL
>Mature_484_residues
METIVLPVINLAPIMPEIILSVFGMALLLVNVFVPSEQKAYLGYLSLVGIIAAGVSIVGGWGVPLDSFSGSVVQDNFALF
FKGIFLLSAGLTILISDHYMKREGCNQGELYPLILFATMGMMLMASGTDLMTVFLGLEVLSVSLYVLAGFNRANLKSNEA
GLKYFLLGAFSTGFLLYGMALTYGATGTTKIPKIAEFVAANGMVAQSPMFYIGMLLMASGFAFKIAAAPFHMWTPDVYEG
APTPMTAFMSAGPKAAGFAAFMRVLIVAFPTLKADWSDLLWVLAVLTMTIGNLIALNQDNIKRMLAYSSIAHAGYALVGF
TAGNAEGAAGILFYMLSYAFMNIGAFAIIILVGKKGEENNNVADYAGFATKHPVLALAMAIFLFSLAGMPPTAGFIGKFY
LFSGAIKAGYIWLAIIGVLNSAASVYYYLRVMVYMYMKNPTEEFDWMTLSPAVMLCILIAVVGVIIPGVVPSYLLELAQK
AVLL

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family [H]

Homologues:

Organism=Escherichia coli, GI145693160, Length=490, Percent_Identity=33.6734693877551, Blast_Score=237, Evalue=2e-63,
Organism=Escherichia coli, GI1788831, Length=402, Percent_Identity=25.1243781094527, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1788614, Length=398, Percent_Identity=25.6281407035176, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788613, Length=199, Percent_Identity=26.6331658291457, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788827, Length=372, Percent_Identity=23.3870967741935, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI2367154, Length=234, Percent_Identity=27.3504273504274, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788829, Length=398, Percent_Identity=22.8643216080402, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010096
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 51890; Mature: 51890

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
6.0 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METIVLPVINLAPIMPEIILSVFGMALLLVNVFVPSEQKAYLGYLSLVGIIAAGVSIVGG
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC
WGVPLDSFSGSVVQDNFALFFKGIFLLSAGLTILISDHYMKREGCNQGELYPLILFATMG
CCCCHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECHHHHHCCCCCCCHHHHHHHHHHH
MMLMASGTDLMTVFLGLEVLSVSLYVLAGFNRANLKSNEAGLKYFLLGAFSTGFLLYGMA
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHH
LTYGATGTTKIPKIAEFVAANGMVAQSPMFYIGMLLMASGFAFKIAAAPFHMWTPDVYEG
HHCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHCC
APTPMTAFMSAGPKAAGFAAFMRVLIVAFPTLKADWSDLLWVLAVLTMTIGNLIALNQDN
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCEEEECHHH
IKRMLAYSSIAHAGYALVGFTAGNAEGAAGILFYMLSYAFMNIGAFAIIILVGKKGEENN
HHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCC
NVADYAGFATKHPVLALAMAIFLFSLAGMPPTAGFIGKFYLFSGAIKAGYIWLAIIGVLN
CCHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAASVYYYLRVMVYMYMKNPTEEFDWMTLSPAVMLCILIAVVGVIIPGVVPSYLLELAQK
HHHHHHHHHHHHHHHHHCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVLL
HHCC
>Mature Secondary Structure
METIVLPVINLAPIMPEIILSVFGMALLLVNVFVPSEQKAYLGYLSLVGIIAAGVSIVGG
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC
WGVPLDSFSGSVVQDNFALFFKGIFLLSAGLTILISDHYMKREGCNQGELYPLILFATMG
CCCCHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECHHHHHCCCCCCCHHHHHHHHHHH
MMLMASGTDLMTVFLGLEVLSVSLYVLAGFNRANLKSNEAGLKYFLLGAFSTGFLLYGMA
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHH
LTYGATGTTKIPKIAEFVAANGMVAQSPMFYIGMLLMASGFAFKIAAAPFHMWTPDVYEG
HHCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHCC
APTPMTAFMSAGPKAAGFAAFMRVLIVAFPTLKADWSDLLWVLAVLTMTIGNLIALNQDN
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCEEEECHHH
IKRMLAYSSIAHAGYALVGFTAGNAEGAAGILFYMLSYAFMNIGAFAIIILVGKKGEENN
HHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCC
NVADYAGFATKHPVLALAMAIFLFSLAGMPPTAGFIGKFYLFSGAIKAGYIWLAIIGVLN
CCHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAASVYYYLRVMVYMYMKNPTEEFDWMTLSPAVMLCILIAVVGVIIPGVVPSYLLELAQK
HHHHHHHHHHHHHHHHHCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVLL
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA