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The map label for this gene is nuoB [H]

Identifier: 39995448

GI number: 39995448

Start: 368210

End: 368722

Strand: Direct

Name: nuoB [H]

Synonym: GSU0339

Alternate gene names: 39995448

Gene position: 368210-368722 (Clockwise)

Preceding gene: 39995447

Following gene: 39995449

Centisome position: 9.65

GC content: 55.95

Gene sequence:

>513_bases
ATGGGAGTAGATCAGCCGCTGGGAGATAACTTCATCACCACGTCCCTGGACAGTCTCGTGAACTGGGCGAGGAAGTCCTC
CATCTGGCCCATGACCTTTGGTCTTGCTTGTTGCGCCATCGAGATGATGGCCACGGGGGCTTCCCATAACGACCTCGACC
GGTTTGGTATCATATTCCGGGCGTCACCCCGGCAGTCGGACTGTATTATTATTGCCGGAACCGTCACCAAGAAGATGCTG
CCGGTAATTAAGACGGTCTATGAGCAGATGCCGGAGCCTAAGTGGGTTGTCGCAATGGGGGCCTGTGCCTGTTCCGGTGG
GGTGTTTGACACGTATAGTGTTGTTCAGGGTATTGACACTGCGCTGCCGGTGGACGTCTATATTCCTGGCTGTCCGCCTC
GTCCCGAGGCTTTGCTGTACGGTCTGCTTAAACTGCAGGACAAGATTATGAAGGACAAGAACTCGTTTGGCTCGACCATA
GGGCTCGGCGAGCGCCTTGAGTCCGCAGCGTGA

Upstream 100 bases:

>100_bases
TGTTTAAGCGGCTTGGGATATTTGGCGTTGTCGAGATGGGTCTGTTCATCGTCATTCTTTTTGTCGGTTACATCTATGTC
TGGAAAAAAGGAGCTCTGGA

Downstream 100 bases:

>100_bases
CCATCTCACTTAAGTAAGGGGAACGAACGGTGATGGCTGAGAACAATCGTGCAGTTATAAAACTTAAGGAAAAATTTGCC
GCGTCAATCCTTGATGTAAG

Product: NADH dehydrogenase I subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B [H]

Number of amino acids: Translated: 170; Mature: 169

Protein sequence:

>170_residues
MGVDQPLGDNFITTSLDSLVNWARKSSIWPMTFGLACCAIEMMATGASHNDLDRFGIIFRASPRQSDCIIIAGTVTKKML
PVIKTVYEQMPEPKWVVAMGACACSGGVFDTYSVVQGIDTALPVDVYIPGCPPRPEALLYGLLKLQDKIMKDKNSFGSTI
GLGERLESAA

Sequences:

>Translated_170_residues
MGVDQPLGDNFITTSLDSLVNWARKSSIWPMTFGLACCAIEMMATGASHNDLDRFGIIFRASPRQSDCIIIAGTVTKKML
PVIKTVYEQMPEPKWVVAMGACACSGGVFDTYSVVQGIDTALPVDVYIPGCPPRPEALLYGLLKLQDKIMKDKNSFGSTI
GLGERLESAA
>Mature_169_residues
GVDQPLGDNFITTSLDSLVNWARKSSIWPMTFGLACCAIEMMATGASHNDLDRFGIIFRASPRQSDCIIIAGTVTKKMLP
VIKTVYEQMPEPKWVVAMGACACSGGVFDTYSVVQGIDTALPVDVYIPGCPPRPEALLYGLLKLQDKIMKDKNSFGSTIG
LGERLESAA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=146, Percent_Identity=60.958904109589, Blast_Score=207, Evalue=5e-54,
Organism=Escherichia coli, GI1788624, Length=160, Percent_Identity=51.875, Blast_Score=188, Evalue=1e-49,
Organism=Escherichia coli, GI1789074, Length=110, Percent_Identity=45.4545454545455, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI1788834, Length=122, Percent_Identity=44.2622950819672, Blast_Score=93, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17509685, Length=147, Percent_Identity=54.421768707483, Blast_Score=190, Evalue=4e-49,
Organism=Drosophila melanogaster, GI18859983, Length=154, Percent_Identity=55.8441558441558, Blast_Score=199, Evalue=7e-52,
Organism=Drosophila melanogaster, GI24642371, Length=154, Percent_Identity=55.8441558441558, Blast_Score=199, Evalue=7e-52,
Organism=Drosophila melanogaster, GI24651058, Length=152, Percent_Identity=53.9473684210526, Blast_Score=194, Evalue=2e-50,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 18367; Mature: 18235

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
8.2 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVDQPLGDNFITTSLDSLVNWARKSSIWPMTFGLACCAIEMMATGASHNDLDRFGIIFR
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHEEEEE
ASPRQSDCIIIAGTVTKKMLPVIKTVYEQMPEPKWVVAMGACACSGGVFDTYSVVQGIDT
CCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHCCCCCHHHHHHHHCCC
ALPVDVYIPGCPPRPEALLYGLLKLQDKIMKDKNSFGSTIGLGERLESAA
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCC
>Mature Secondary Structure 
GVDQPLGDNFITTSLDSLVNWARKSSIWPMTFGLACCAIEMMATGASHNDLDRFGIIFR
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHEEEEE
ASPRQSDCIIIAGTVTKKMLPVIKTVYEQMPEPKWVVAMGACACSGGVFDTYSVVQGIDT
CCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHCCCCCHHHHHHHHCCC
ALPVDVYIPGCPPRPEALLYGLLKLQDKIMKDKNSFGSTIGLGERLESAA
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA