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The map label for this gene is zraR [H]

Identifier: 39995409

GI number: 39995409

Start: 329817

End: 330251

Strand: Direct

Name: zraR [H]

Synonym: GSU0300

Alternate gene names: 39995409

Gene position: 329817-330251 (Clockwise)

Preceding gene: 39995407

Following gene: 39995410

Centisome position: 8.65

GC content: 54.25

Gene sequence:

>435_bases
ATGGGACAGCAGTGGGTCAACACCTTCCTCGACCGCTTCAACCGCAACAAGAAGAGCAAGGTAAAGGGCATCGACGCCGA
CGCCATGGAGATACTCTGCGGCTACGACTGGCCCGGCAACGTGCGCGAGCTGGAAAACCTGGTGGAACGGCTCGTCATCC
TCAAGGGGTTCGGCACTCTCGGGGTCAAGGATCTGCCGGAAAAGTACACCGGTGTTTCGCTGTCCGCACCGTCCGAAACC
CTGGCGCTTCCCGATGCGGGTATCTGTCTCAATACCGTTGTTGAAGAGTTTGAGAACAATCTGATTCTGCAGGCTCTCAA
GAAGACCGGCGGCAACAAGAAAGAGGCGGCACTGCTGCTCAATCTGAAACGGACTACCCTGATCGAAAAATTAAAAAAGA
GAAAGCTCGATGTAATTTCCACGTCTCTTGCATAG

Upstream 100 bases:

>100_bases
ACAATGTGCCACGGCTCTGATCTCCTTGTTAATATTGGTGTTTTGGCGAACTCAATATATCACAAGTAGCTCTCAGGGCC
TCTCTTTATTCAGTTAAACT

Downstream 100 bases:

>100_bases
ACGCGGGTATGTTCATCGACCCGACACAACGCCCCAACCTTCAAACCGACACTGCAGCCGGGGAGCGCCCCTCCGTCACC
GGCGTGGCGGATACCTCGCG

Product: sigma-54 dependent DNA-binding response regulator, interruption-N

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 144; Mature: 143

Protein sequence:

>144_residues
MGQQWVNTFLDRFNRNKKSKVKGIDADAMEILCGYDWPGNVRELENLVERLVILKGFGTLGVKDLPEKYTGVSLSAPSET
LALPDAGICLNTVVEEFENNLILQALKKTGGNKKEAALLLNLKRTTLIEKLKKRKLDVISTSLA

Sequences:

>Translated_144_residues
MGQQWVNTFLDRFNRNKKSKVKGIDADAMEILCGYDWPGNVRELENLVERLVILKGFGTLGVKDLPEKYTGVSLSAPSET
LALPDAGICLNTVVEEFENNLILQALKKTGGNKKEAALLLNLKRTTLIEKLKKRKLDVISTSLA
>Mature_143_residues
GQQWVNTFLDRFNRNKKSKVKGIDADAMEILCGYDWPGNVRELENLVERLVILKGFGTLGVKDLPEKYTGVSLSAPSETL
ALPDAGICLNTVVEEFENNLILQALKKTGGNKKEAALLLNLKRTTLIEKLKKRKLDVISTSLA

Specific function: Member of the two-component regulatory system zraS/zraR. When activated by zraS it acts in conjunction with sigma-54 to regulate the expression of zraP. Positively autoregulates the expression of the zraSR operon [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=125, Percent_Identity=42.4, Blast_Score=86, Evalue=7e-19,
Organism=Escherichia coli, GI1788550, Length=131, Percent_Identity=30.5343511450382, Blast_Score=74, Evalue=3e-15,
Organism=Escherichia coli, GI1789087, Length=131, Percent_Identity=35.8778625954198, Blast_Score=64, Evalue=5e-12,
Organism=Escherichia coli, GI87082117, Length=140, Percent_Identity=30, Blast_Score=61, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 15988; Mature: 15857

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQQWVNTFLDRFNRNKKSKVKGIDADAMEILCGYDWPGNVRELENLVERLVILKGFGTL
CCHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCC
GVKDLPEKYTGVSLSAPSETLALPDAGICLNTVVEEFENNLILQALKKTGGNKKEAALLL
CCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
NLKRTTLIEKLKKRKLDVISTSLA
HHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GQQWVNTFLDRFNRNKKSKVKGIDADAMEILCGYDWPGNVRELENLVERLVILKGFGTL
CHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCC
GVKDLPEKYTGVSLSAPSETLALPDAGICLNTVVEEFENNLILQALKKTGGNKKEAALLL
CCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
NLKRTTLIEKLKKRKLDVISTSLA
HHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]