Click here to switch to the map view.

The map label for this gene is glpF [H]

Identifier: 38234772

GI number: 38234772

Start: 2324605

End: 2325345

Strand: Reverse

Name: glpF [H]

Synonym: DIP2236

Alternate gene names: 38234772

Gene position: 2325345-2324605 (Counterclockwise)

Preceding gene: 38234773

Following gene: 38234771

Centisome position: 93.44

GC content: 60.86

Gene sequence:

>741_bases
ATGACCGCCCTACAAGCATTCGGCTGGGAGTTTTTGGGCACAGCCCTCCTGCTTCTCCTCGGTAACGGCGTTTGCGCCGT
CAACAGCCTGCGCACCTCCGCAGGCAAAGGCACTGGCTGGGTGCTTATCGCTTTCGGCTGGGGCATGGCGGTATTCGTCG
GTGCCTCAGTGGCAAGCCCCAGCGGCGGCCACCTCAACCCAGCGGTCACTATCGCGCTGGCGATCAAAGGCAGCACACCA
TGGAACCTGGTAGGCGGCTATGTTCTCGGGCAGTTCCTCGGAGCCATGTTTGGTGCAATCCTGTGCTGGCTGACATTCAA
GCAGCTTTTCGACGCCAACAACACCGACGAAAACGGAAACGTCACGGGAGCTAACCGCACCACCGGCGGTATCTTCTTCA
CCGGCCCCGCCCACAATCAGAACGGGTGGAACATGGTCACCGAGTTCATCGGCACCTGCGTACTGCTGCTGTTCATCTTC
TTCGGCCCCACCGGCGGCGACCTAGGCCCCATGACCTATTTCGCAGTCGCCTTTGTGATCGTTGCCATAGGCTTGTCGCT
AGGCACACCGACCGGCTACGCCATCAACCCTGTGCGTGACCTCGGCCCCCGCCTGATGTACGCCTTCGTGCTGCCCATTA
AAGACAAAGGATCCGCGAACTGGGGCTATGCCTGGGTTCCGATCGTCGGCCCCATGATCGCCGCAGTCTTCTGCGGAGTG
CTGGCCACTGTAGTGCTGTAG

Upstream 100 bases:

>100_bases
AAATCGCCGCCGAGAAGGAGCTCACTGACAAAGCCGCCAACGAGCACATCGTGGCAGGCTCCGCGGTGCGCAGCTATGTG
GATGTGTCAGGTGATAACTA

Downstream 100 bases:

>100_bases
TGAAAGGGAACCACACCAATGAATCAACCACAGTATGTTGCTGCGATTGACCAAGGAACGACGTCGACACGCTGCATCAT
TTTCGACCACAACGGCCAAC

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MTALQAFGWEFLGTALLLLLGNGVCAVNSLRTSAGKGTGWVLIAFGWGMAVFVGASVASPSGGHLNPAVTIALAIKGSTP
WNLVGGYVLGQFLGAMFGAILCWLTFKQLFDANNTDENGNVTGANRTTGGIFFTGPAHNQNGWNMVTEFIGTCVLLLFIF
FGPTGGDLGPMTYFAVAFVIVAIGLSLGTPTGYAINPVRDLGPRLMYAFVLPIKDKGSANWGYAWVPIVGPMIAAVFCGV
LATVVL

Sequences:

>Translated_246_residues
MTALQAFGWEFLGTALLLLLGNGVCAVNSLRTSAGKGTGWVLIAFGWGMAVFVGASVASPSGGHLNPAVTIALAIKGSTP
WNLVGGYVLGQFLGAMFGAILCWLTFKQLFDANNTDENGNVTGANRTTGGIFFTGPAHNQNGWNMVTEFIGTCVLLLFIF
FGPTGGDLGPMTYFAVAFVIVAIGLSLGTPTGYAINPVRDLGPRLMYAFVLPIKDKGSANWGYAWVPIVGPMIAAVFCGV
LATVVL
>Mature_245_residues
TALQAFGWEFLGTALLLLLGNGVCAVNSLRTSAGKGTGWVLIAFGWGMAVFVGASVASPSGGHLNPAVTIALAIKGSTPW
NLVGGYVLGQFLGAMFGAILCWLTFKQLFDANNTDENGNVTGANRTTGGIFFTGPAHNQNGWNMVTEFIGTCVLLLFIFF
GPTGGDLGPMTYFAVAFVIVAIGLSLGTPTGYAINPVRDLGPRLMYAFVLPIKDKGSANWGYAWVPIVGPMIAAVFCGVL
ATVVL

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI157266307, Length=253, Percent_Identity=34.7826086956522, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI4826645, Length=243, Percent_Identity=35.3909465020576, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI22538420, Length=232, Percent_Identity=34.4827586206897, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI4502187, Length=260, Percent_Identity=31.9230769230769, Blast_Score=83, Evalue=2e-16,
Organism=Escherichia coli, GI1790362, Length=236, Percent_Identity=31.7796610169492, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71994009, Length=238, Percent_Identity=33.6134453781513, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17531429, Length=239, Percent_Identity=34.3096234309623, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17531431, Length=239, Percent_Identity=34.3096234309623, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI32564052, Length=240, Percent_Identity=31.25, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17533613, Length=240, Percent_Identity=31.25, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71992966, Length=231, Percent_Identity=29.4372294372294, Blast_Score=81, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17544068, Length=246, Percent_Identity=31.3008130081301, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17558372, Length=197, Percent_Identity=30.9644670050761, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71992961, Length=228, Percent_Identity=27.1929824561404, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6321054, Length=208, Percent_Identity=35.0961538461538, Blast_Score=95, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6322985, Length=290, Percent_Identity=28.9655172413793, Blast_Score=79, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 25649; Mature: 25518

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALQAFGWEFLGTALLLLLGNGVCAVNSLRTSAGKGTGWVLIAFGWGMAVFVGASVASP
CCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHCCCCCCCEEEEEEHHHHHHHHHCCCCCC
SGGHLNPAVTIALAIKGSTPWNLVGGYVLGQFLGAMFGAILCWLTFKQLFDANNTDENGN
CCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
VTGANRTTGGIFFTGPAHNQNGWNMVTEFIGTCVLLLFIFFGPTGGDLGPMTYFAVAFVI
EECCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
VAIGLSLGTPTGYAINPVRDLGPRLMYAFVLPIKDKGSANWGYAWVPIVGPMIAAVFCGV
HHHHHHCCCCCCCEECCHHHHHHHEEEHEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHH
LATVVL
HHHHHC
>Mature Secondary Structure 
TALQAFGWEFLGTALLLLLGNGVCAVNSLRTSAGKGTGWVLIAFGWGMAVFVGASVASP
CCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHCCCCCCCEEEEEEHHHHHHHHHCCCCCC
SGGHLNPAVTIALAIKGSTPWNLVGGYVLGQFLGAMFGAILCWLTFKQLFDANNTDENGN
CCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
VTGANRTTGGIFFTGPAHNQNGWNMVTEFIGTCVLLLFIFFGPTGGDLGPMTYFAVAFVI
EECCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
VAIGLSLGTPTGYAINPVRDLGPRLMYAFVLPIKDKGSANWGYAWVPIVGPMIAAVFCGV
HHHHHHCCCCCCCEECCHHHHHHHEEEHEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHH
LATVVL
HHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12000953; 3225846 [H]