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The map label for this gene is nadE [H]

Identifier: 38234443

GI number: 38234443

Start: 1917354

End: 1918172

Strand: Direct

Name: nadE [H]

Synonym: DIP1873

Alternate gene names: 38234443

Gene position: 1917354-1918172 (Clockwise)

Preceding gene: 38234441

Following gene: 38234449

Centisome position: 77.04

GC content: 58.73

Gene sequence:

>819_bases
ATGCGCAACAACATCATCCACCGACTTGGAACTAAACCCACCATCGACCCCGCAACGGAAATCCGCAACCGCGTCGAATT
TCTCACCCGTTACATCAAGCACTCTGGCACCAAAGGTTTCGCGCTAGGGATCTCCGGCGGACAAGACTCCACCCTCGCAG
GCCGCCTCGCACAGCTCGCAGTAGAGCAGCTACGAGTAGCCGGCTACCCCGCCGAATTCTGGGCAATCCGCCTCCCCTAC
GGTGTACAAGCCGACGAGGATGACGCCCACATCGCCCTAGAATTTATCCAGCCCGATCACAGCGTGGTCATCAACATTAA
AGAGGCGACCGACGCAGCCGCCCGCGCCACCGCCCAAGCATTGGGGCTTCAAGACGTAGGCGACTTCAATAAAGGCAACA
TCAAAGCCCGCCAACGCATGATCGCTCAATACGCACTCGCCGGCGAAAAGAAACTACTCGTGATCGGCACCGACCACGCA
GCAGAAAACGTCACCGGATTCTTTACAAAGTTCGGCGATGGCGGCGCCGACATCCTCCCGCTTGCAGGGCTATCTAAACG
CCAAGGCGCCGCCCTCCTAGAACACCTCGGCGCACCAGCATCCACATGGGAAAAAGTCCCCACTGCCGACCTCGAAGAGG
ATCGCCCAGCACTTCCCGACGAAGAAGCACTCGGCGTGACCTACCGAGAGATCGACGCTTACATCGAAGGAAACGAAGAA
GTCTCACCCGAGGCACAACAGCGCATCGAGCGCCTCTGGAAAATCGGCCAACACAAACGCCACCTCCCCGTCGAACCCGA
TGACACTTGGTGGCACTAG

Upstream 100 bases:

>100_bases
AAAGTCTGCCATGACACGAGGGACAATACTATCCCATAGCACGACCAAATGCCAAACCGTGCCACTTGGCGCATAAGATT
ACACCATGAGCACTTCCCCA

Downstream 100 bases:

>100_bases
TAGCGCTCTGGTTCCTCCCCAAATACGAACGCTCGGCCAGACACCTCGCGGACGTCGATACGCACAAAGTTGTACTTCAA
CGTTGGCAGCCATGGTTTCA

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MRNNIIHRLGTKPTIDPATEIRNRVEFLTRYIKHSGTKGFALGISGGQDSTLAGRLAQLAVEQLRVAGYPAEFWAIRLPY
GVQADEDDAHIALEFIQPDHSVVINIKEATDAAARATAQALGLQDVGDFNKGNIKARQRMIAQYALAGEKKLLVIGTDHA
AENVTGFFTKFGDGGADILPLAGLSKRQGAALLEHLGAPASTWEKVPTADLEEDRPALPDEEALGVTYREIDAYIEGNEE
VSPEAQQRIERLWKIGQHKRHLPVEPDDTWWH

Sequences:

>Translated_272_residues
MRNNIIHRLGTKPTIDPATEIRNRVEFLTRYIKHSGTKGFALGISGGQDSTLAGRLAQLAVEQLRVAGYPAEFWAIRLPY
GVQADEDDAHIALEFIQPDHSVVINIKEATDAAARATAQALGLQDVGDFNKGNIKARQRMIAQYALAGEKKLLVIGTDHA
AENVTGFFTKFGDGGADILPLAGLSKRQGAALLEHLGAPASTWEKVPTADLEEDRPALPDEEALGVTYREIDAYIEGNEE
VSPEAQQRIERLWKIGQHKRHLPVEPDDTWWH
>Mature_272_residues
MRNNIIHRLGTKPTIDPATEIRNRVEFLTRYIKHSGTKGFALGISGGQDSTLAGRLAQLAVEQLRVAGYPAEFWAIRLPY
GVQADEDDAHIALEFIQPDHSVVINIKEATDAAARATAQALGLQDVGDFNKGNIKARQRMIAQYALAGEKKLLVIGTDHA
AENVTGFFTKFGDGGADILPLAGLSKRQGAALLEHLGAPASTWEKVPTADLEEDRPALPDEEALGVTYREIDAYIEGNEE
VSPEAQQRIERLWKIGQHKRHLPVEPDDTWWH

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788036, Length=274, Percent_Identity=50.7299270072993, Blast_Score=261, Evalue=5e-71,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 29928; Mature: 29928

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNNIIHRLGTKPTIDPATEIRNRVEFLTRYIKHSGTKGFALGISGGQDSTLAGRLAQLA
CCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHH
VEQLRVAGYPAEFWAIRLPYGVQADEDDAHIALEFIQPDHSVVINIKEATDAAARATAQA
HHHHHHCCCCHHHEEEECCCCCCCCCCCCEEEEEEECCCCEEEEEEHHHHHHHHHHHHHH
LGLQDVGDFNKGNIKARQRMIAQYALAGEKKLLVIGTDHAAENVTGFFTKFGDGGADILP
HCCHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCEEE
LAGLSKRQGAALLEHLGAPASTWEKVPTADLEEDRPALPDEEALGVTYREIDAYIEGNEE
CCCCCCCCHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHCCCCCC
VSPEAQQRIERLWKIGQHKRHLPVEPDDTWWH
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRNNIIHRLGTKPTIDPATEIRNRVEFLTRYIKHSGTKGFALGISGGQDSTLAGRLAQLA
CCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHH
VEQLRVAGYPAEFWAIRLPYGVQADEDDAHIALEFIQPDHSVVINIKEATDAAARATAQA
HHHHHHCCCCHHHEEEECCCCCCCCCCCCEEEEEEECCCCEEEEEEHHHHHHHHHHHHHH
LGLQDVGDFNKGNIKARQRMIAQYALAGEKKLLVIGTDHAAENVTGFFTKFGDGGADILP
HCCHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCEEE
LAGLSKRQGAALLEHLGAPASTWEKVPTADLEEDRPALPDEEALGVTYREIDAYIEGNEE
CCCCCCCCHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHCCCCCC
VSPEAQQRIERLWKIGQHKRHLPVEPDDTWWH
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]