Click here to switch to the map view.

The map label for this gene is pheS

Identifier: 38233755

GI number: 38233755

Start: 1155546

End: 1156601

Strand: Direct

Name: pheS

Synonym: DIP1165

Alternate gene names: 38233755

Gene position: 1155546-1156601 (Clockwise)

Preceding gene: 38233754

Following gene: 38233756

Centisome position: 46.43

GC content: 50.47

Gene sequence:

>1056_bases
GTGTCTGAACAACCGGACTATCCCGCTGTCGAGCTGACTGAGGAGGGCCTAGCGCAAGCTGCTGATGCCGCGATTCGTGC
CTTTGGCGTTGCTTCAAACTTAGATGAGTTGAATATTGCTCGTCGAGATCATCTAGGTGATGCGGCGCCGATTCCTCAAG
CGCGACGTAGCCTAGGCACTCTGCCTAAAGATCAGCGTAAAGATGCCGGTCGTCTTGTTAACATGGCTCGGGGCCGTTTA
GAGAAACACTTTGCTCAGGTAAAAGAAGTCTTGGAAGCTAAACATCTCGAAGAGATGCTGAAGGCTGAGCGGGTGGACGT
CACTGTTCCCACAACGCGTCATCAGACAGGTGCTCTACACCCAATTACGACCCTTAGTGAAAAGATTGCGGATATCTTTG
TAGCAATGGGCTACGAGGTGGCAGACGGGCCTGAAATAGAAGCAGAGTACTTCAACTTCGATTCGCTAAATTTCATCCCT
GATCACCCGGCCCGTACGTTGCAAGATACTTTCCATGTCGGTGAGCCTGGTTCGAGTCAAGTTTTGCGCACTCACACTTC
ACCTGTGCAAATGCGAACCATGTTGTCCCGTGAGGTGCCGATCTATGTTGTGTGCCCAGGCCGCGTGTTTCGCACGGATG
AATTAGATGCGACACATACTCCAGTTTTCCATCAGGTAGAAGGTCTTGCTGTGGATAAGGGCCTAACTATGGCTCATCTG
CGTGGCACACTTGATCATTTGGCTAAGACTTTGTTTGGCCCTGAAACAAAAACCCGTATGCGTGCCAATTACTTCCCATT
TACTGAACCATCAGCTGAAGTGGATGTTTGGTTCCCCAATAAAAAGGGCGGTGCTGGTTGGATCGAGTGGGGTGGCTGCG
GCATGGTGAACCCTAACGTGTTGAAAGCCGCAGGTATTGACCCAGAAGTGTATTCAGGATTCGCTTTTGGAATGGGATTG
GAACGTACTTTGCAGTTCCGAAATGGACTGACTGATATGCGTGACATGGTGGAAGGCGATGTTCGTTTCACTCTTCCATT
TGGTGTTCAGGCGTAG

Upstream 100 bases:

>100_bases
TTACTTTCCAGTAGTGGGTGAGTTGGCTCTTGGCTTGGCATAACGGGGTATGATGTCATGGTTATCTATAACTTCTGCTA
ACTATGACGAGGAGCATCGA

Downstream 100 bases:

>100_bases
AGCAATTCGAGTATCCAAAGAATAAACAGGAGTTAGATTACATGCTCATTTCACAAAACTGGGTCACCGAATTGTTAGGT
CGATCGAACCCAGATTGGAA

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MSEQPDYPAVELTEEGLAQAADAAIRAFGVASNLDELNIARRDHLGDAAPIPQARRSLGTLPKDQRKDAGRLVNMARGRL
EKHFAQVKEVLEAKHLEEMLKAERVDVTVPTTRHQTGALHPITTLSEKIADIFVAMGYEVADGPEIEAEYFNFDSLNFIP
DHPARTLQDTFHVGEPGSSQVLRTHTSPVQMRTMLSREVPIYVVCPGRVFRTDELDATHTPVFHQVEGLAVDKGLTMAHL
RGTLDHLAKTLFGPETKTRMRANYFPFTEPSAEVDVWFPNKKGGAGWIEWGGCGMVNPNVLKAAGIDPEVYSGFAFGMGL
ERTLQFRNGLTDMRDMVEGDVRFTLPFGVQA

Sequences:

>Translated_351_residues
MSEQPDYPAVELTEEGLAQAADAAIRAFGVASNLDELNIARRDHLGDAAPIPQARRSLGTLPKDQRKDAGRLVNMARGRL
EKHFAQVKEVLEAKHLEEMLKAERVDVTVPTTRHQTGALHPITTLSEKIADIFVAMGYEVADGPEIEAEYFNFDSLNFIP
DHPARTLQDTFHVGEPGSSQVLRTHTSPVQMRTMLSREVPIYVVCPGRVFRTDELDATHTPVFHQVEGLAVDKGLTMAHL
RGTLDHLAKTLFGPETKTRMRANYFPFTEPSAEVDVWFPNKKGGAGWIEWGGCGMVNPNVLKAAGIDPEVYSGFAFGMGL
ERTLQFRNGLTDMRDMVEGDVRFTLPFGVQA
>Mature_350_residues
SEQPDYPAVELTEEGLAQAADAAIRAFGVASNLDELNIARRDHLGDAAPIPQARRSLGTLPKDQRKDAGRLVNMARGRLE
KHFAQVKEVLEAKHLEEMLKAERVDVTVPTTRHQTGALHPITTLSEKIADIFVAMGYEVADGPEIEAEYFNFDSLNFIPD
HPARTLQDTFHVGEPGSSQVLRTHTSPVQMRTMLSREVPIYVVCPGRVFRTDELDATHTPVFHQVEGLAVDKGLTMAHLR
GTLDHLAKTLFGPETKTRMRANYFPFTEPSAEVDVWFPNKKGGAGWIEWGGCGMVNPNVLKAAGIDPEVYSGFAFGMGLE
RTLQFRNGLTDMRDMVEGDVRFTLPFGVQA

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=274, Percent_Identity=31.021897810219, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI5729820, Length=274, Percent_Identity=28.1021897810219, Blast_Score=99, Evalue=4e-21,
Organism=Escherichia coli, GI1788007, Length=331, Percent_Identity=44.7129909365559, Blast_Score=280, Evalue=9e-77,
Organism=Caenorhabditis elegans, GI17508957, Length=274, Percent_Identity=31.3868613138686, Blast_Score=130, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI32563657, Length=274, Percent_Identity=31.3868613138686, Blast_Score=130, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI32566635, Length=309, Percent_Identity=29.4498381877023, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6321087, Length=263, Percent_Identity=28.8973384030418, Blast_Score=121, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6325304, Length=276, Percent_Identity=26.8115942028986, Blast_Score=99, Evalue=7e-22,
Organism=Drosophila melanogaster, GI18858079, Length=273, Percent_Identity=31.8681318681319, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI17137424, Length=287, Percent_Identity=26.8292682926829, Blast_Score=99, Evalue=3e-21,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_CORDI (Q6NHH2)

Other databases:

- EMBL:   BX248357
- RefSeq:   NP_939522.1
- ProteinModelPortal:   Q6NHH2
- SMR:   Q6NHH2
- GeneID:   2648565
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1165
- NMPDR:   fig|257309.1.peg.1113
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- PhylomeDB:   Q6NHH2
- ProtClustDB:   PRK00488
- BioCyc:   CDIP257309:DIP1165-MONOMER
- BRENDA:   6.1.1.20
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 38852; Mature: 38720

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQPDYPAVELTEEGLAQAADAAIRAFGVASNLDELNIARRDHLGDAAPIPQARRSLGT
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHCC
LPKDQRKDAGRLVNMARGRLEKHFAQVKEVLEAKHLEEMLKAERVDVTVPTTRHQTGALH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCCCC
PITTLSEKIADIFVAMGYEVADGPEIEAEYFNFDSLNFIPDHPARTLQDTFHVGEPGSSQ
HHHHHHHHHHHHHHHCCCEECCCCCCCCEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCC
VLRTHTSPVQMRTMLSREVPIYVVCPGRVFRTDELDATHTPVFHQVEGLAVDKGLTMAHL
EEEECCCHHHHHHHHHCCCCEEEECCCCEEECCCCCCCCCCHHHHHCCEEECCCCHHHHH
RGTLDHLAKTLFGPETKTRMRANYFPFTEPSAEVDVWFPNKKGGAGWIEWGGCGMVNPNV
HHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCEEEECCCCCCCCCC
LKAAGIDPEVYSGFAFGMGLERTLQFRNGLTDMRDMVEGDVRFTLPFGVQA
EEECCCCHHHHCCHHHCCCHHHHHHHHCCHHHHHHHHCCCEEEEECCCCCC
>Mature Secondary Structure 
SEQPDYPAVELTEEGLAQAADAAIRAFGVASNLDELNIARRDHLGDAAPIPQARRSLGT
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHCC
LPKDQRKDAGRLVNMARGRLEKHFAQVKEVLEAKHLEEMLKAERVDVTVPTTRHQTGALH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCCCC
PITTLSEKIADIFVAMGYEVADGPEIEAEYFNFDSLNFIPDHPARTLQDTFHVGEPGSSQ
HHHHHHHHHHHHHHHCCCEECCCCCCCCEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCC
VLRTHTSPVQMRTMLSREVPIYVVCPGRVFRTDELDATHTPVFHQVEGLAVDKGLTMAHL
EEEECCCHHHHHHHHHCCCCEEEECCCCEEECCCCCCCCCCHHHHHCCEEECCCCHHHHH
RGTLDHLAKTLFGPETKTRMRANYFPFTEPSAEVDVWFPNKKGGAGWIEWGGCGMVNPNV
HHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCEEEECCCCCCCCCC
LKAAGIDPEVYSGFAFGMGLERTLQFRNGLTDMRDMVEGDVRFTLPFGVQA
EEECCCCHHHHCCHHHCCCHHHHHHHHCCHHHHHHHHCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910