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The map label for this gene is hupA [H]

Identifier: 38233279

GI number: 38233279

Start: 648378

End: 649634

Strand: Direct

Name: hupA [H]

Synonym: DIP0672

Alternate gene names: 38233279

Gene position: 648378-649634 (Clockwise)

Preceding gene: 38233278

Following gene: 38233280

Centisome position: 26.05

GC content: 55.85

Gene sequence:

>1257_bases
ATGAATATTGACGCCGGCTGGCAAGGGGAAACCCTCCAGGACAACCTAGAGCGTCGTGGTGTTAGTCGCCGTGATTTTAT
GAAGCTCTGTAGCGGATTAGCTGCAGTATTTACCTTTGGTGCTCCCATTAGCGCGCGGTCAGAAGAGCTGAAATCTAGCG
CCGAGGAGATCGCTAAAAAACTTGGTGCCGTAACCAAACCCAATGTTGCATGGTTGCAGCTACAAGAGTGCACCGGCTGC
ATGGAATCGGTACTGCGATCCGGTGGCACCACCGTAGAAGACATGGTCTTGGGACTGCTCTCCTTGAACTACAACGAGCT
GGTCATGGCGGCCTCCGGCGAAGCAGCTGAAAAAGCGCTCGAAGAAGTCTCCCACGAGCCACATATCTTGGTAGTCAACG
GTTCCGTACCGTTGAATGAAAACGGCGTGTACTGCACCATCGGCGGAAAGTCCGCTGAGCAGGTACTGCGCGAGGCGGCA
GAAAACGCTACGGCGGTGCTCGCTGTCGGTGCATGCGCAGTCTATGGTTCTGTTCAGGCTGCCAAGCCCAATCCCACAGG
TGCGGTAGGTGTAGAGGACATCATTACTGATAAACCAGTGATCAACGTATCCGGTTGCCCACCAATTGGCGAGGTTATTA
CCGCCACCATCACTTACATTCTTACCCACGGTGAAGCACCGAAGGTGGATGCCGAAGGTCGTCCGCTTTTCGCCTACGAC
CAGCGTATTCACGATTCCTGCCCACGTCGCGCGCACTACGATGCCGGCCAGTTCGTGCGCTCCTTTGATGACGACGGCGC
TAAAAACGGTTGGTGCCTCTACGAAGTCGGTTGTAAGGGCCCATCGACGTTTAGCCCGTGCCCAATCATCCAGTGGAATA
TGAAAGCCGGCTGGCCAATTGGTGCAGGTCACCCATGTATCGGCTGCACGGAAAAAGACTTCTTTGATAAATTCACGCCG
TTTTACTCAGTGCTGCCCAATATCAAAGGCATTGGTATCGAGTCGACAACTGAGAAGATCGGACTGGGCCTCATGGGCGC
AGCCGCAGCAGGCGTGGCGGTCCACGGCGGTATTACCGCGCTGCGTTCGCAAAAAGAAAACCGGCTCAGCCGAGAAAACG
ACGTTGCTCTAGCAGCCTTCGGTGACGGCCCAGGGCCTGTTGCGCTGCCGTTCCCCACGGTCAACGCCGCCGGAAATGGT
CATGGGGGCGTCGATAAGCGCGGACAATCAGCATCTAGCAAGAATGAGGAGGCGTAG

Upstream 100 bases:

>100_bases
GGTGAAATGATATTCTTTCAGTGAAAAGTGCCGCATGTACGTTAGTGTTGAACAGGGCACTGGGTGTATTGGTTACTACC
CCCGAGGAGGGGCTGCTGTT

Downstream 100 bases:

>100_bases
AGCTAATGGCTGAGCGCGTAGTTATTGATCCACTCACCCGAATTGAAGGCCACCTGCGAATCGAGCTAGAACGCGATGGC
AAAAACATTTCCAACGCGTG

Product: putative uptake hydrogenase small subunit

Products: NA

Alternate protein names: Hydrogenlyase; Membrane-bound hydrogenase small subunit [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MNIDAGWQGETLQDNLERRGVSRRDFMKLCSGLAAVFTFGAPISARSEELKSSAEEIAKKLGAVTKPNVAWLQLQECTGC
MESVLRSGGTTVEDMVLGLLSLNYNELVMAASGEAAEKALEEVSHEPHILVVNGSVPLNENGVYCTIGGKSAEQVLREAA
ENATAVLAVGACAVYGSVQAAKPNPTGAVGVEDIITDKPVINVSGCPPIGEVITATITYILTHGEAPKVDAEGRPLFAYD
QRIHDSCPRRAHYDAGQFVRSFDDDGAKNGWCLYEVGCKGPSTFSPCPIIQWNMKAGWPIGAGHPCIGCTEKDFFDKFTP
FYSVLPNIKGIGIESTTEKIGLGLMGAAAAGVAVHGGITALRSQKENRLSRENDVALAAFGDGPGPVALPFPTVNAAGNG
HGGVDKRGQSASSKNEEA

Sequences:

>Translated_418_residues
MNIDAGWQGETLQDNLERRGVSRRDFMKLCSGLAAVFTFGAPISARSEELKSSAEEIAKKLGAVTKPNVAWLQLQECTGC
MESVLRSGGTTVEDMVLGLLSLNYNELVMAASGEAAEKALEEVSHEPHILVVNGSVPLNENGVYCTIGGKSAEQVLREAA
ENATAVLAVGACAVYGSVQAAKPNPTGAVGVEDIITDKPVINVSGCPPIGEVITATITYILTHGEAPKVDAEGRPLFAYD
QRIHDSCPRRAHYDAGQFVRSFDDDGAKNGWCLYEVGCKGPSTFSPCPIIQWNMKAGWPIGAGHPCIGCTEKDFFDKFTP
FYSVLPNIKGIGIESTTEKIGLGLMGAAAAGVAVHGGITALRSQKENRLSRENDVALAAFGDGPGPVALPFPTVNAAGNG
HGGVDKRGQSASSKNEEA
>Mature_418_residues
MNIDAGWQGETLQDNLERRGVSRRDFMKLCSGLAAVFTFGAPISARSEELKSSAEEIAKKLGAVTKPNVAWLQLQECTGC
MESVLRSGGTTVEDMVLGLLSLNYNELVMAASGEAAEKALEEVSHEPHILVVNGSVPLNENGVYCTIGGKSAEQVLREAA
ENATAVLAVGACAVYGSVQAAKPNPTGAVGVEDIITDKPVINVSGCPPIGEVITATITYILTHGEAPKVDAEGRPLFAYD
QRIHDSCPRRAHYDAGQFVRSFDDDGAKNGWCLYEVGCKGPSTFSPCPIIQWNMKAGWPIGAGHPCIGCTEKDFFDKFTP
FYSVLPNIKGIGIESTTEKIGLGLMGAAAAGVAVHGGITALRSQKENRLSRENDVALAAFGDGPGPVALPFPTVNAAGNG
HGGVDKRGQSASSKNEEA

Specific function: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions [H]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787206, Length=340, Percent_Identity=43.8235294117647, Blast_Score=292, Evalue=3e-80,
Organism=Escherichia coli, GI1789371, Length=302, Percent_Identity=43.7086092715232, Blast_Score=260, Evalue=1e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 43949; Mature: 43949

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIDAGWQGETLQDNLERRGVSRRDFMKLCSGLAAVFTFGAPISARSEELKSSAEEIAKK
CCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LGAVTKPNVAWLQLQECTGCMESVLRSGGTTVEDMVLGLLSLNYNELVMAASGEAAEKAL
HCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCHHHHHHH
EEVSHEPHILVVNGSVPLNENGVYCTIGGKSAEQVLREAAENATAVLAVGACAVYGSVQA
HHHCCCCEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHCCCEEEEEHHHHHHHCCCCC
AKPNPTGAVGVEDIITDKPVINVSGCPPIGEVITATITYILTHGEAPKVDAEGRPLFAYD
CCCCCCCCCCHHHHHCCCCEEEECCCCCHHHHHHHHHHHHEECCCCCCCCCCCCCEEHHH
QRIHDSCPRRAHYDAGQFVRSFDDDGAKNGWCLYEVGCKGPSTFSPCPIIQWNMKAGWPI
HHHHHCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCEEEECCCCCCCC
GAGHPCIGCTEKDFFDKFTPFYSVLPNIKGIGIESTTEKIGLGLMGAAAAGVAVHGGITA
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCHHHHHHHCCHHHCCHHH
LRSQKENRLSRENDVALAAFGDGPGPVALPFPTVNAAGNGHGGVDKRGQSASSKNEEA
HHHHHHHHCCCCCCEEEEEECCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNIDAGWQGETLQDNLERRGVSRRDFMKLCSGLAAVFTFGAPISARSEELKSSAEEIAKK
CCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LGAVTKPNVAWLQLQECTGCMESVLRSGGTTVEDMVLGLLSLNYNELVMAASGEAAEKAL
HCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCHHHHHHH
EEVSHEPHILVVNGSVPLNENGVYCTIGGKSAEQVLREAAENATAVLAVGACAVYGSVQA
HHHCCCCEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHCCCEEEEEHHHHHHHCCCCC
AKPNPTGAVGVEDIITDKPVINVSGCPPIGEVITATITYILTHGEAPKVDAEGRPLFAYD
CCCCCCCCCCHHHHHCCCCEEEECCCCCHHHHHHHHHHHHEECCCCCCCCCCCCCEEHHH
QRIHDSCPRRAHYDAGQFVRSFDDDGAKNGWCLYEVGCKGPSTFSPCPIIQWNMKAGWPI
HHHHHCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCEEEECCCCCCCC
GAGHPCIGCTEKDFFDKFTPFYSVLPNIKGIGIESTTEKIGLGLMGAAAAGVAVHGGITA
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCHHHHHHHCCHHHCCHHH
LRSQKENRLSRENDVALAAFGDGPGPVALPFPTVNAAGNGHGGVDKRGQSASSKNEEA
HHHHHHHHCCCCCCEEEEEECCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3054886; 12597275 [H]