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The map label for this gene is hupB [H]

Identifier: 38233280

GI number: 38233280

Start: 649640

End: 651385

Strand: Direct

Name: hupB [H]

Synonym: DIP0673

Alternate gene names: 38233280

Gene position: 649640-651385 (Clockwise)

Preceding gene: 38233279

Following gene: 38233281

Centisome position: 26.1

GC content: 55.33

Gene sequence:

>1746_bases
ATGGCTGAGCGCGTAGTTATTGATCCACTCACCCGAATTGAAGGCCACCTGCGAATCGAGCTAGAACGCGATGGCAAAAA
CATTTCCAACGCGTGGAGCGAAACCACCCAGTTCCGCGGCATCGAAACCATTGTAAAAGGCCGCGACCCACGTGACGTGT
GGGCATTCGTTGGTCGTATCTGTGGCGTGTGTACCTCTACACACTCCGTTGCAGCAGTCATGGCAGTAGAAAACGCCATC
GGTTCCCAAGTACCTAAACAAGCAGAACTGATCCGCGACATTTTGCTTGGCGCACAAGAAATCCACGACCACGTGGTGCA
CTTCTACCACCTGCACGCTCTTGACTGGGTCAACGTTGTTTCGGCGGCAGACGCGGACCCCAAAAAGGCCGTTGAGTTTG
CTAATAAGATCGGCTCCAAGTGGAAGGGCAACACCGAATCACAATTTGCCAAGGTGAAAAAGACCGTCCAAGGCATCCTC
GATTCAGGACAATTGTCCATCTTCACTGGCGGCTACTGGGACCACCCCGACTACCGTCTTCCCGCAGAAGCCAACCTGAT
GGCGGTATCGCACTACCTCGACGCCCTAGAATTCCAGCGCTCGATCATTCGTATCACCACCATCTTCGGTGGCAAAAACC
CACACCCCAACTTCCTTGTTGGCGGCATGGCCTGCAACATCGACCCCAACAAGTCTGAGACGTTTAACCAAGTACAACTT
GATCAAATCAAAGATTGGACCGATGAGATCCAAGAATTTGTCAAGGAATGCTACTACCCAGATGCTGTCGCCATCATGGG
TGCCTACAAGGACTACTTCAAGATCGGTGCATCCTCGCCAAACTTCCTAGCCATCGGCATGGCAGGTACACGTTACGCAG
GTGGGGTAGTAGGAACACCACGTTCCCACAGCAACCGCGATATCAAACCGGGCGTGATCCTCGACGGCGACTACAGCAAG
GTCCACCCCCTCGATCACGACAAGATCCGTGAGTACATCTCCTCAGCGTGGTACAGCTACGAAGAAGGCGACGACGCTGG
CTTGCAGCCACTCGTCGGTGAAACAACCGTCAACTACACCGGCCCAACCCCGCCATACACATGGCTTGCCGACGACGATA
AGTACACTTGGTCTAAAGCACCGCGTTACGACGGCCGCCCCACCCAAGTAGGCCCTGTTGCCCGCGTGCTCAGCGCCTAC
ATCCAAGGCCACGCACACACGCGCAAGCTTGTCGACGACGCCCTGCGCAGCGTAGACATGCAGGTTAAGGACCTCAATTC
CACAGCGGGTCGTACCTTGGCACGTGCAATCGAAGCCATCACCACTGCCGATCACATGGCAGTCGATCTGCTACCAGCCT
TCGTCCAAGGAATCGTTGGCGGCGACTACGAAGTCTTCGACTCATCCAAGTGGGAGCCAGATACGTGGCCTAAAGAAGCC
TCTGATGGTTTCGGACTGATGGAAGTTGCTCGTGGCTGCCTCGGACACTACGTCACCATCGAAGACCAAAAAGTAACCCG
CTACCAGGCGGTTGTTCCTACAACCTGGCTTGCCGGTGGCCGTGACCCACAAGGCAACATGGGCCCTTATGAGGAATCAC
TCGCCGGCGGCGGACACCCACTGCTTGATCCAAAGCAGCCTTTGGAACCACTACGCACCATCCACTCCTTTGACCCATGC
ATGTCCTGCGCAGTCCATGTTCTCGATGTGGAGGGACACGAAGAATTCCAGGTGATGACGTCATGA

Upstream 100 bases:

>100_bases
CGCTGCCGTTCCCCACGGTCAACGCCGCCGGAAATGGTCATGGGGGCGTCGATAAGCGCGGACAATCAGCATCTAGCAAG
AATGAGGAGGCGTAGAGCTA

Downstream 100 bases:

>100_bases
AACTCATCCACGTCGCACAGTCCTACAGTGTGCGCAAGCTCAGCCCAGGGCGTCTGCTCGCCTTGGCTGCTGCGGCACCT
GAGCATACTGAGGACCCCAT

Product: putative uptake hydrogenase large subunit

Products: NA

Alternate protein names: Hydrogenlyase; Membrane-bound hydrogenase large subunit [H]

Number of amino acids: Translated: 581; Mature: 580

Protein sequence:

>581_residues
MAERVVIDPLTRIEGHLRIELERDGKNISNAWSETTQFRGIETIVKGRDPRDVWAFVGRICGVCTSTHSVAAVMAVENAI
GSQVPKQAELIRDILLGAQEIHDHVVHFYHLHALDWVNVVSAADADPKKAVEFANKIGSKWKGNTESQFAKVKKTVQGIL
DSGQLSIFTGGYWDHPDYRLPAEANLMAVSHYLDALEFQRSIIRITTIFGGKNPHPNFLVGGMACNIDPNKSETFNQVQL
DQIKDWTDEIQEFVKECYYPDAVAIMGAYKDYFKIGASSPNFLAIGMAGTRYAGGVVGTPRSHSNRDIKPGVILDGDYSK
VHPLDHDKIREYISSAWYSYEEGDDAGLQPLVGETTVNYTGPTPPYTWLADDDKYTWSKAPRYDGRPTQVGPVARVLSAY
IQGHAHTRKLVDDALRSVDMQVKDLNSTAGRTLARAIEAITTADHMAVDLLPAFVQGIVGGDYEVFDSSKWEPDTWPKEA
SDGFGLMEVARGCLGHYVTIEDQKVTRYQAVVPTTWLAGGRDPQGNMGPYEESLAGGGHPLLDPKQPLEPLRTIHSFDPC
MSCAVHVLDVEGHEEFQVMTS

Sequences:

>Translated_581_residues
MAERVVIDPLTRIEGHLRIELERDGKNISNAWSETTQFRGIETIVKGRDPRDVWAFVGRICGVCTSTHSVAAVMAVENAI
GSQVPKQAELIRDILLGAQEIHDHVVHFYHLHALDWVNVVSAADADPKKAVEFANKIGSKWKGNTESQFAKVKKTVQGIL
DSGQLSIFTGGYWDHPDYRLPAEANLMAVSHYLDALEFQRSIIRITTIFGGKNPHPNFLVGGMACNIDPNKSETFNQVQL
DQIKDWTDEIQEFVKECYYPDAVAIMGAYKDYFKIGASSPNFLAIGMAGTRYAGGVVGTPRSHSNRDIKPGVILDGDYSK
VHPLDHDKIREYISSAWYSYEEGDDAGLQPLVGETTVNYTGPTPPYTWLADDDKYTWSKAPRYDGRPTQVGPVARVLSAY
IQGHAHTRKLVDDALRSVDMQVKDLNSTAGRTLARAIEAITTADHMAVDLLPAFVQGIVGGDYEVFDSSKWEPDTWPKEA
SDGFGLMEVARGCLGHYVTIEDQKVTRYQAVVPTTWLAGGRDPQGNMGPYEESLAGGGHPLLDPKQPLEPLRTIHSFDPC
MSCAVHVLDVEGHEEFQVMTS
>Mature_580_residues
AERVVIDPLTRIEGHLRIELERDGKNISNAWSETTQFRGIETIVKGRDPRDVWAFVGRICGVCTSTHSVAAVMAVENAIG
SQVPKQAELIRDILLGAQEIHDHVVHFYHLHALDWVNVVSAADADPKKAVEFANKIGSKWKGNTESQFAKVKKTVQGILD
SGQLSIFTGGYWDHPDYRLPAEANLMAVSHYLDALEFQRSIIRITTIFGGKNPHPNFLVGGMACNIDPNKSETFNQVQLD
QIKDWTDEIQEFVKECYYPDAVAIMGAYKDYFKIGASSPNFLAIGMAGTRYAGGVVGTPRSHSNRDIKPGVILDGDYSKV
HPLDHDKIREYISSAWYSYEEGDDAGLQPLVGETTVNYTGPTPPYTWLADDDKYTWSKAPRYDGRPTQVGPVARVLSAYI
QGHAHTRKLVDDALRSVDMQVKDLNSTAGRTLARAIEAITTADHMAVDLLPAFVQGIVGGDYEVFDSSKWEPDTWPKEAS
DGFGLMEVARGCLGHYVTIEDQKVTRYQAVVPTTWLAGGRDPQGNMGPYEESLAGGGHPLLDPKQPLEPLRTIHSFDPCM
SCAVHVLDVEGHEEFQVMTS

Specific function: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=589, Percent_Identity=40.7470288624788, Blast_Score=456, Evalue=1e-129,
Organism=Escherichia coli, GI1789368, Length=592, Percent_Identity=39.1891891891892, Blast_Score=421, Evalue=1e-119,
Organism=Escherichia coli, GI1789076, Length=96, Percent_Identity=37.5, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 64246; Mature: 64115

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAERVVIDPLTRIEGHLRIELERDGKNISNAWSETTQFRGIETIVKGRDPRDVWAFVGRI
CCCCEEECCHHHCCCEEEEEEECCCCCHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHH
CGVCTSTHSVAAVMAVENAIGSQVPKQAELIRDILLGAQEIHDHVVHFYHLHALDWVNVV
HHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAADADPKKAVEFANKIGSKWKGNTESQFAKVKKTVQGILDSGQLSIFTGGYWDHPDYRL
HHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCC
PAEANLMAVSHYLDALEFQRSIIRITTIFGGKNPHPNFLVGGMACNIDPNKSETFNQVQL
CCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCEEEECEEECCCCCCCCCCCHHHH
DQIKDWTDEIQEFVKECYYPDAVAIMGAYKDYFKIGASSPNFLAIGMAGTRYAGGVVGTP
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCC
RSHSNRDIKPGVILDGDYSKVHPLDHDKIREYISSAWYSYEEGDDAGLQPLVGETTVNYT
CCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCC
GPTPPYTWLADDDKYTWSKAPRYDGRPTQVGPVARVLSAYIQGHAHTRKLVDDALRSVDM
CCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCC
QVKDLNSTAGRTLARAIEAITTADHMAVDLLPAFVQGIVGGDYEVFDSSKWEPDTWPKEA
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCC
SDGFGLMEVARGCLGHYVTIEDQKVTRYQAVVPTTWLAGGRDPQGNMGPYEESLAGGGHP
CCCCCHHHHHHHHHCCEEEEECCHHHHHHEECCHHCCCCCCCCCCCCCCCHHHHCCCCCC
LLDPKQPLEPLRTIHSFDPCMSCAVHVLDVEGHEEFQVMTS
CCCCCCHHHHHHHHHCCCHHHHHHEEEEECCCCCCEEEECC
>Mature Secondary Structure 
AERVVIDPLTRIEGHLRIELERDGKNISNAWSETTQFRGIETIVKGRDPRDVWAFVGRI
CCCEEECCHHHCCCEEEEEEECCCCCHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHH
CGVCTSTHSVAAVMAVENAIGSQVPKQAELIRDILLGAQEIHDHVVHFYHLHALDWVNVV
HHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAADADPKKAVEFANKIGSKWKGNTESQFAKVKKTVQGILDSGQLSIFTGGYWDHPDYRL
HHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCC
PAEANLMAVSHYLDALEFQRSIIRITTIFGGKNPHPNFLVGGMACNIDPNKSETFNQVQL
CCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCEEEECEEECCCCCCCCCCCHHHH
DQIKDWTDEIQEFVKECYYPDAVAIMGAYKDYFKIGASSPNFLAIGMAGTRYAGGVVGTP
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCC
RSHSNRDIKPGVILDGDYSKVHPLDHDKIREYISSAWYSYEEGDDAGLQPLVGETTVNYT
CCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCC
GPTPPYTWLADDDKYTWSKAPRYDGRPTQVGPVARVLSAYIQGHAHTRKLVDDALRSVDM
CCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCC
QVKDLNSTAGRTLARAIEAITTADHMAVDLLPAFVQGIVGGDYEVFDSSKWEPDTWPKEA
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCC
SDGFGLMEVARGCLGHYVTIEDQKVTRYQAVVPTTWLAGGRDPQGNMGPYEESLAGGGHP
CCCCCHHHHHHHHHCCEEEEECCHHHHHHEECCHHCCCCCCCCCCCCCCCHHHHCCCCCC
LLDPKQPLEPLRTIHSFDPCMSCAVHVLDVEGHEEFQVMTS
CCCCCCHHHHHHHHHCCCHHHHHHEEEEECCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3054886; 12597275; 8163174 [H]