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The map label for this gene is hypB [H]

Identifier: 38233278

GI number: 38233278

Start: 647316

End: 648077

Strand: Direct

Name: hypB [H]

Synonym: DIP0671

Alternate gene names: 38233278

Gene position: 647316-648077 (Clockwise)

Preceding gene: 38233277

Following gene: 38233279

Centisome position: 26.01

GC content: 53.02

Gene sequence:

>762_bases
ATGGGGCGTTTTCATCGCCACGACGACGGTACGGTGCATTCCCACGACCATGACCACCACGGTCATTCTCATGATGTTGG
GGATCACTCCGGTTACGAAACTGGGCGGGAGCGCATTGAGGTCCTAGAGGATATTTTCCACGAAAACGACCACTGCGCTG
CGGATAACAAGGAGCTTTTCGACGCCCACCAGGTGCGTTGCATCAACATCATGAGCTCGCCTGGTGCAGGTAAAACCACG
GTGTTGCAGCATGTATTGCGAGCTGCTGAAGGAAAAGTGCGCGTAGGTGTTGTTGAAGGCGACATTGAAACTTCCCTAGA
CGCAGATCGTCTCACTGGCTTTGGTGCCCAGATTTCATTGCTGAACACTGGCAATGGTTTTGGTGGAGAGTGCCACCTCG
ATGCCCCTATGGTGAGCCGTGCGCTTCGTGGCCTAGACCTTGACACCCTCGACGTGGTGCTCATTGAAAACGTGGGCAAC
TTGGTGTGCCCTGCGGAATTTGAAGTCGGTGAGCACAGCAAAGCGATGATCTACTCGGTCACGGAGGGGGAAGATAAGCC
TCTGAAGTACCCTGTGATGTTCCGTAGCGTGGATGCAGTCGTGATTAACAAGATCGACTTGCTGCCATATTTGGATTTTG
ACATGGAGCTTTTTATGAAGAATCTGCGCCAAGTCAATCCCGACGCCAAAGTGTTTGAGGTTTCTGCCAAGACGGGTGAA
GGACTCGACGGCTGGATTGAATGGTTGGTCGGGGCCAAATAG

Upstream 100 bases:

>100_bases
GTTTAGTTGTGCCACTTGTGGTGCGCCCGCTGAGGTAATCAGTGGGGAAGAGTTCACAGTTATGGACATAGAAGTCGAGC
AATCCTAGGAGGAGTTTTTC

Downstream 100 bases:

>100_bases
ACGAGTAAGTAATCTGATCTCTTAGGGCGAGTCTACCCCACAAAGTGGGTGTGCTCGCCCTTTGTATTTTCGACACAATT
TAGTTGTGAAAAACGGTGTG

Product: putative hydrogenase nickel incorporation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MGRFHRHDDGTVHSHDHDHHGHSHDVGDHSGYETGRERIEVLEDIFHENDHCAADNKELFDAHQVRCINIMSSPGAGKTT
VLQHVLRAAEGKVRVGVVEGDIETSLDADRLTGFGAQISLLNTGNGFGGECHLDAPMVSRALRGLDLDTLDVVLIENVGN
LVCPAEFEVGEHSKAMIYSVTEGEDKPLKYPVMFRSVDAVVINKIDLLPYLDFDMELFMKNLRQVNPDAKVFEVSAKTGE
GLDGWIEWLVGAK

Sequences:

>Translated_253_residues
MGRFHRHDDGTVHSHDHDHHGHSHDVGDHSGYETGRERIEVLEDIFHENDHCAADNKELFDAHQVRCINIMSSPGAGKTT
VLQHVLRAAEGKVRVGVVEGDIETSLDADRLTGFGAQISLLNTGNGFGGECHLDAPMVSRALRGLDLDTLDVVLIENVGN
LVCPAEFEVGEHSKAMIYSVTEGEDKPLKYPVMFRSVDAVVINKIDLLPYLDFDMELFMKNLRQVNPDAKVFEVSAKTGE
GLDGWIEWLVGAK
>Mature_252_residues
GRFHRHDDGTVHSHDHDHHGHSHDVGDHSGYETGRERIEVLEDIFHENDHCAADNKELFDAHQVRCINIMSSPGAGKTTV
LQHVLRAAEGKVRVGVVEGDIETSLDADRLTGFGAQISLLNTGNGFGGECHLDAPMVSRALRGLDLDTLDVVLIENVGNL
VCPAEFEVGEHSKAMIYSVTEGEDKPLKYPVMFRSVDAVVINKIDLLPYLDFDMELFMKNLRQVNPDAKVFEVSAKTGEG
LDGWIEWLVGAK

Specific function: Required for the maturation of the three [NiFe]hydrogenases. Exhibits a low intrinsic GTPase activity. The GTP hydrolysis catalyzed by HypB is an integral process in the incorporation of nickel into hydrogenases [H]

COG id: COG0378

COG function: function code OK; Ni2+-binding GTPase involved in regulation of expression and maturation of urease and hydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypB/hupM family [H]

Homologues:

Organism=Escherichia coli, GI1789082, Length=231, Percent_Identity=44.5887445887446, Blast_Score=204, Evalue=7e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003495
- InterPro:   IPR004392
- InterPro:   IPR012202 [H]

Pfam domain/function: PF02492 cobW [H]

EC number: NA

Molecular weight: Translated: 27948; Mature: 27816

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRFHRHDDGTVHSHDHDHHGHSHDVGDHSGYETGRERIEVLEDIFHENDHCAADNKELF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
DAHQVRCINIMSSPGAGKTTVLQHVLRAAEGKVRVGVVEGDIETSLDADRLTGFGAQISL
HHHCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHCCCCCEEEE
LNTGNGFGGECHLDAPMVSRALRGLDLDTLDVVLIENVGNLVCPAEFEVGEHSKAMIYSV
EECCCCCCCEEEECCHHHHHHHHCCCCCHHHEEEEECCCCEECCCCCCCCCCCCEEEEEE
TEGEDKPLKYPVMFRSVDAVVINKIDLLPYLDFDMELFMKNLRQVNPDAKVFEVSAKTGE
ECCCCCCEECCHHHHHHCCHHHHHHHCCEECCCCHHHHHHHHHHCCCCCEEEEEECCCCC
GLDGWIEWLVGAK
CHHHHHHHHHCCC
>Mature Secondary Structure 
GRFHRHDDGTVHSHDHDHHGHSHDVGDHSGYETGRERIEVLEDIFHENDHCAADNKELF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
DAHQVRCINIMSSPGAGKTTVLQHVLRAAEGKVRVGVVEGDIETSLDADRLTGFGAQISL
HHHCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHCCCCCEEEE
LNTGNGFGGECHLDAPMVSRALRGLDLDTLDVVLIENVGNLVCPAEFEVGEHSKAMIYSV
EECCCCCCCEEEECCHHHHHHHHCCCCCHHHEEEEECCCCEECCCCCCCCCCCCEEEEEE
TEGEDKPLKYPVMFRSVDAVVINKIDLLPYLDFDMELFMKNLRQVNPDAKVFEVSAKTGE
ECCCCCCEECCHHHHHHCCHHHHHHHCCEECCCCHHHHHHHHHHCCCCCEEEEEECCCCC
GLDGWIEWLVGAK
CHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]