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The map label for this gene is rlmN [H]

Identifier: 37678948

GI number: 37678948

Start: 767916

End: 769040

Strand: Direct

Name: rlmN [H]

Synonym: VV0764

Alternate gene names: 37678948

Gene position: 767916-769040 (Clockwise)

Preceding gene: 37678947

Following gene: 37678949

Centisome position: 22.89

GC content: 47.11

Gene sequence:

>1125_bases
ATGAGCACTGAAAAAATTAATCTACTCGACTTTGATCGCAAAGGCCTGCGTGAACTGTTCGCCCAAGAGTTAGGCGAAAA
AGCGTTTCGTGCTGATCAGGTAATGAAGTGGATCTACCATTTCGGTGTCGATGATTTCGATAACATGACCAACATCAACA
AGCAGTTGAGAGAGAAGCTTAAGCAGAAATGCGAGATCGTCGCACCTGTTGTTTCAGAAGCTCAACACTCATCAGATGGC
ACCATCAAGTGGGCAATGCGTGTTGGTGACCAAGATGTTGAAACGGTCTATATCCCAGAAGAAGATCGCGCAACGCTTTG
TGTTTCATCTCAAGTGGGTTGTGCACTGGAATGTAAATTCTGTTCAACGGCGCAGCAAGGTTTCAACCGTAACTTAAAAG
TGTCAGAAATCATCGGTCAGGTGTGGCGTGCGGCACGTGAAGTGGGGCTTGAGAAAGAGACTGGGCGTCGTCCGATTACC
AACGTGGTGATGATGGGCATGGGCGAACCGTTGCTGAACATGAAAAACCTCATTCCTGCTTTGGAAATTATGCTGGATGA
TCTAGGCTTTGGCCTATCTAAACGCCGCGTTACTGTGTCGACTTCGGGTGTGGTTTCTGGCTTAGATCAGATGACGGGCA
AAATTGACGTTGCACTGGCGATTTCTTTGCATGCACCTAATGACAAATTGCGCAGTGAAATTATGCCGATCAACGATCGT
TGGGATATTCAGGATTTCTTAGCATCAGTGCGTCGCTACATTGCTTCTTCCAATGCCAACCGCGGTAAAGTGACGGTGGA
ATACGTGCTACTTGATCATGTTAACGATGGCACTGAACATGCCCATGAGCTTGCGCAGTTGATGAAGGATACACCTTGTA
AAATCAACTTGATTCCATTTAACCCATACCCAGGATCACCGTATAAGAAACCAAGCAACTCACGTATTGATCGTTTCCAA
AAGACATTGATGCAATATGAGCATACGGTGACGATTCGTAAGACGCGCGGTGATGATATTGATGCCGCATGTGGTCAGTT
AGTGGGGGACGTGATCGACCGAACCAAGCGTACCGCCATGCTGAAAGCGGCCAAAGGTGAGACCATCGAGGTTAAAGCGC
TGTAA

Upstream 100 bases:

>100_bases
TTATTGCCTTAGCGTCTTCGGGGTTTTACAGCCCTTGTTGTCACTGCATAAAGCCTGTACAATTCGCGCCCTTAATCTTG
TTCAGTCATTGAGAGGCACC

Downstream 100 bases:

>100_bases
GCCATCATTTTCTTATTAAACAGCCAGAGCTACGCTCTGGCTGTTTTGCTTTCTAATCGTCATTTTTTGCTTGTTTGGTG
GACTTTCCATGCTGAATTGG

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MSTEKINLLDFDRKGLRELFAQELGEKAFRADQVMKWIYHFGVDDFDNMTNINKQLREKLKQKCEIVAPVVSEAQHSSDG
TIKWAMRVGDQDVETVYIPEEDRATLCVSSQVGCALECKFCSTAQQGFNRNLKVSEIIGQVWRAAREVGLEKETGRRPIT
NVVMMGMGEPLLNMKNLIPALEIMLDDLGFGLSKRRVTVSTSGVVSGLDQMTGKIDVALAISLHAPNDKLRSEIMPINDR
WDIQDFLASVRRYIASSNANRGKVTVEYVLLDHVNDGTEHAHELAQLMKDTPCKINLIPFNPYPGSPYKKPSNSRIDRFQ
KTLMQYEHTVTIRKTRGDDIDAACGQLVGDVIDRTKRTAMLKAAKGETIEVKAL

Sequences:

>Translated_374_residues
MSTEKINLLDFDRKGLRELFAQELGEKAFRADQVMKWIYHFGVDDFDNMTNINKQLREKLKQKCEIVAPVVSEAQHSSDG
TIKWAMRVGDQDVETVYIPEEDRATLCVSSQVGCALECKFCSTAQQGFNRNLKVSEIIGQVWRAAREVGLEKETGRRPIT
NVVMMGMGEPLLNMKNLIPALEIMLDDLGFGLSKRRVTVSTSGVVSGLDQMTGKIDVALAISLHAPNDKLRSEIMPINDR
WDIQDFLASVRRYIASSNANRGKVTVEYVLLDHVNDGTEHAHELAQLMKDTPCKINLIPFNPYPGSPYKKPSNSRIDRFQ
KTLMQYEHTVTIRKTRGDDIDAACGQLVGDVIDRTKRTAMLKAAKGETIEVKAL
>Mature_373_residues
STEKINLLDFDRKGLRELFAQELGEKAFRADQVMKWIYHFGVDDFDNMTNINKQLREKLKQKCEIVAPVVSEAQHSSDGT
IKWAMRVGDQDVETVYIPEEDRATLCVSSQVGCALECKFCSTAQQGFNRNLKVSEIIGQVWRAAREVGLEKETGRRPITN
VVMMGMGEPLLNMKNLIPALEIMLDDLGFGLSKRRVTVSTSGVVSGLDQMTGKIDVALAISLHAPNDKLRSEIMPINDRW
DIQDFLASVRRYIASSNANRGKVTVEYVLLDHVNDGTEHAHELAQLMKDTPCKINLIPFNPYPGSPYKKPSNSRIDRFQK
TLMQYEHTVTIRKTRGDDIDAACGQLVGDVIDRTKRTAMLKAAKGETIEVKAL

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=370, Percent_Identity=73.5135135135135, Blast_Score=577, Evalue=1e-166,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 42005; Mature: 41874

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTEKINLLDFDRKGLRELFAQELGEKAFRADQVMKWIYHFGVDDFDNMTNINKQLREKL
CCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
KQKCEIVAPVVSEAQHSSDGTIKWAMRVGDQDVETVYIPEEDRATLCVSSQVGCALECKF
HHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEECCCCCCEEEEECCCCCEEEEHH
CSTAQQGFNRNLKVSEIIGQVWRAAREVGLEKETGRRPITNVVMMGMGEPLLNMKNLIPA
HHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHCCCCHHHHHHHHHHH
LEIMLDDLGFGLSKRRVTVSTSGVVSGLDQMTGKIDVALAISLHAPNDKLRSEIMPINDR
HHHHHHHHCCCCCCCEEEEECCHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHCCCCCCC
WDIQDFLASVRRYIASSNANRGKVTVEYVLLDHVNDGTEHAHELAQLMKDTPCKINLIPF
CCHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEC
NPYPGSPYKKPSNSRIDRFQKTLMQYEHTVTIRKTRGDDIDAACGQLVGDVIDRTKRTAM
CCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
LKAAKGETIEVKAL
HHHCCCCEEEEEEC
>Mature Secondary Structure 
STEKINLLDFDRKGLRELFAQELGEKAFRADQVMKWIYHFGVDDFDNMTNINKQLREKL
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
KQKCEIVAPVVSEAQHSSDGTIKWAMRVGDQDVETVYIPEEDRATLCVSSQVGCALECKF
HHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEECCCCCCEEEEECCCCCEEEEHH
CSTAQQGFNRNLKVSEIIGQVWRAAREVGLEKETGRRPITNVVMMGMGEPLLNMKNLIPA
HHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHCCCCHHHHHHHHHHH
LEIMLDDLGFGLSKRRVTVSTSGVVSGLDQMTGKIDVALAISLHAPNDKLRSEIMPINDR
HHHHHHHHCCCCCCCEEEEECCHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHCCCCCCC
WDIQDFLASVRRYIASSNANRGKVTVEYVLLDHVNDGTEHAHELAQLMKDTPCKINLIPF
CCHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEC
NPYPGSPYKKPSNSRIDRFQKTLMQYEHTVTIRKTRGDDIDAACGQLVGDVIDRTKRTAM
CCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
LKAAKGETIEVKAL
HHHCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA