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The map label for this gene is insB [H]

Identifier: 37678743

GI number: 37678743

Start: 540468

End: 540893

Strand: Direct

Name: insB [H]

Synonym: VV0559

Alternate gene names: 37678743

Gene position: 540468-540893 (Clockwise)

Preceding gene: 37678742

Following gene: 37678749

Centisome position: 16.11

GC content: 42.49

Gene sequence:

>426_bases
GTGACCTCGATTCCATTCGATAAAGCAAATATCTCTCTCATCTGTGAAGTTGATGAGCAATGGTCATTCGTCGGTAAAAA
GAAAAATCGCCGCTGGCTTTGGTATGCTTGGGAGCCTCGCTATAAACGCATCATTGCTCACGCATTTGGTAAACGAAATA
CGGATGCTCTTCGATCGTTACTCACTTTGCTAAAGCCGTTCGACATCAGCTTCTTTTTTACCGATGACTTCAGCGTTTAC
GCAAAAGAATTACCAAAGGAAAAGCATGTTGTAGGCAAAAGGTTCACTCAGCGGATTGAGCGAACCAATCTGACCTTACG
TTCCAGATTAAAACGTTTAGTGCGTAGAACTATCGGCTTTTCTAGATCGGAGGAGATGCACGATAAGGTGATCGGAACAT
TCATTGAACGGGAATTCTATTTTTAA

Upstream 100 bases:

>100_bases
ATATGGCAATGAATAGCTCTGGTGTAAGAGAAACAAGCCGTGTCTTAAAAGTTGGCTATAACACAGTGCTACGCACTTTA
AAAAACTCTCGCCAAGGCAA

Downstream 100 bases:

>100_bases
ATCAACCGATTGGAGTCATGACCCTTTTCTTCTCGCGAGTGATACATTTCCCTCGGCAGAAGCGGTGATATGATCGCTCC
ACCAGCACATGAGCTTTCTG

Product: putative transposase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 141; Mature: 140

Protein sequence:

>141_residues
MTSIPFDKANISLICEVDEQWSFVGKKKNRRWLWYAWEPRYKRIIAHAFGKRNTDALRSLLTLLKPFDISFFFTDDFSVY
AKELPKEKHVVGKRFTQRIERTNLTLRSRLKRLVRRTIGFSRSEEMHDKVIGTFIEREFYF

Sequences:

>Translated_141_residues
MTSIPFDKANISLICEVDEQWSFVGKKKNRRWLWYAWEPRYKRIIAHAFGKRNTDALRSLLTLLKPFDISFFFTDDFSVY
AKELPKEKHVVGKRFTQRIERTNLTLRSRLKRLVRRTIGFSRSEEMHDKVIGTFIEREFYF
>Mature_140_residues
TSIPFDKANISLICEVDEQWSFVGKKKNRRWLWYAWEPRYKRIIAHAFGKRNTDALRSLLTLLKPFDISFFFTDDFSVYA
KELPKEKHVVGKRFTQRIERTNLTLRSRLKRLVRRTIGFSRSEEMHDKVIGTFIEREFYF

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1789853, Length=132, Percent_Identity=44.6969696969697, Blast_Score=132, Evalue=7e-33,
Organism=Escherichia coli, GI1788203, Length=132, Percent_Identity=44.6969696969697, Blast_Score=132, Evalue=7e-33,
Organism=Escherichia coli, GI1786203, Length=132, Percent_Identity=44.6969696969697, Blast_Score=132, Evalue=7e-33,
Organism=Escherichia coli, GI1787223, Length=132, Percent_Identity=44.6969696969697, Blast_Score=130, Evalue=2e-32,
Organism=Escherichia coli, GI1786470, Length=132, Percent_Identity=44.6969696969697, Blast_Score=130, Evalue=4e-32,
Organism=Escherichia coli, GI1786459, Length=132, Percent_Identity=44.6969696969697, Blast_Score=130, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 17058; Mature: 16927

Theoretical pI: Translated: 10.79; Mature: 10.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSIPFDKANISLICEVDEQWSFVGKKKNRRWLWYAWEPRYKRIIAHAFGKRNTDALRSL
CCCCCCCCCCEEEEEEECCHHHHHCCCCCCEEEEEEECHHHHHHHHHHHCCCCHHHHHHH
LTLLKPFDISFFFTDDFSVYAKELPKEKHVVGKRFTQRIERTNLTLRSRLKRLVRRTIGF
HHHHCCCCEEEEEECCHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
SRSEEMHDKVIGTFIEREFYF
CCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TSIPFDKANISLICEVDEQWSFVGKKKNRRWLWYAWEPRYKRIIAHAFGKRNTDALRSL
CCCCCCCCCEEEEEEECCHHHHHCCCCCCEEEEEEECHHHHHHHHHHHCCCCHHHHHHH
LTLLKPFDISFFFTDDFSVYAKELPKEKHVVGKRFTQRIERTNLTLRSRLKRLVRRTIGF
HHHHCCCCEEEEEECCHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
SRSEEMHDKVIGTFIEREFYF
CCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6265806 [H]