Click here to switch to the map view.

The map label for this gene is insA [H]

Identifier: 37678742

GI number: 37678742

Start: 540196

End: 540471

Strand: Direct

Name: insA [H]

Synonym: VV0558

Alternate gene names: 37678742

Gene position: 540196-540471 (Clockwise)

Preceding gene: 37678741

Following gene: 37678743

Centisome position: 16.1

GC content: 40.22

Gene sequence:

>276_bases
ATGGCTACCATTCAAGCTCAATGTCGATTTTGCAACCAAACTGAACATGTTCGTAAACACGGAACTGGAGTAACAGGCTT
TCAGCGCTTTCGTTGCATTGAGTGCAAGCGTTCTTTTCAGCTTGATTATGTATATGAGGCATATAAGCCTAATGTCAAAA
AACAAATCATTGATATGGCAATGAATAGCTCTGGTGTAAGAGAAACAAGCCGTGTCTTAAAAGTTGGCTATAACACAGTG
CTACGCACTTTAAAAAACTCTCGCCAAGGCAAGTGA

Upstream 100 bases:

>100_bases
TGTTGAAACCATGCTGGCAATGAACCCAAATTTGGGTCGTGACTCCAACATGGAGATGTTGTGTTAAAATGGTAGCCTTT
TCACCCTCAAATTAGAAATC

Downstream 100 bases:

>100_bases
CCTCGATTCCATTCGATAAAGCAAATATCTCTCTCATCTGTGAAGTTGATGAGCAATGGTCATTCGTCGGTAAAAAGAAA
AATCGCCGCTGGCTTTGGTA

Product: putative transposase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 91; Mature: 90

Protein sequence:

>91_residues
MATIQAQCRFCNQTEHVRKHGTGVTGFQRFRCIECKRSFQLDYVYEAYKPNVKKQIIDMAMNSSGVRETSRVLKVGYNTV
LRTLKNSRQGK

Sequences:

>Translated_91_residues
MATIQAQCRFCNQTEHVRKHGTGVTGFQRFRCIECKRSFQLDYVYEAYKPNVKKQIIDMAMNSSGVRETSRVLKVGYNTV
LRTLKNSRQGK
>Mature_90_residues
ATIQAQCRFCNQTEHVRKHGTGVTGFQRFRCIECKRSFQLDYVYEAYKPNVKKQIIDMAMNSSGVRETSRVLKVGYNTVL
RTLKNSRQGK

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG3677

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS1 elements insA family [H]

Homologues:

Organism=Escherichia coli, GI1790747, Length=87, Percent_Identity=45.9770114942529, Blast_Score=90, Evalue=3e-20,
Organism=Escherichia coli, GI1789852, Length=87, Percent_Identity=43.6781609195402, Blast_Score=90, Evalue=4e-20,
Organism=Escherichia coli, GI1788204, Length=87, Percent_Identity=43.6781609195402, Blast_Score=90, Evalue=4e-20,
Organism=Escherichia coli, GI1786204, Length=87, Percent_Identity=43.6781609195402, Blast_Score=90, Evalue=4e-20,
Organism=Escherichia coli, GI87081814, Length=87, Percent_Identity=42.5287356321839, Blast_Score=88, Evalue=1e-19,
Organism=Escherichia coli, GI1786471, Length=87, Percent_Identity=42.5287356321839, Blast_Score=88, Evalue=1e-19,
Organism=Escherichia coli, GI1786460, Length=87, Percent_Identity=42.5287356321839, Blast_Score=88, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003220 [H]

Pfam domain/function: PF03811 Ins_element1 [H]

EC number: NA

Molecular weight: Translated: 10550; Mature: 10419

Theoretical pI: Translated: 10.57; Mature: 10.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
4.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIQAQCRFCNQTEHVRKHGTGVTGFQRFRCIECKRSFQLDYVYEAYKPNVKKQIIDMA
CCCCHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHH
MNSSGVRETSRVLKVGYNTVLRTLKNSRQGK
HCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ATIQAQCRFCNQTEHVRKHGTGVTGFQRFRCIECKRSFQLDYVYEAYKPNVKKQIIDMA
CCCHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHH
MNSSGVRETSRVLKVGYNTVLRTLKNSRQGK
HCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6265806 [H]