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The map label for this gene is insH10 [H]

Identifier: 37678715

GI number: 37678715

Start: 514731

End: 515789

Strand: Direct

Name: insH10 [H]

Synonym: VV0531

Alternate gene names: 37678715

Gene position: 514731-515789 (Clockwise)

Preceding gene: 37678714

Following gene: 37678720

Centisome position: 15.34

GC content: 47.21

Gene sequence:

>1059_bases
ATGCATGAAGATAAAGAGCTATTTGATAAGCTTAATCTGTCAGTGTACGAGATAGTGTTCTACCGGGCCTTGGTTGAAAA
TGAGGCTTCAGTACATGAACTTGGCTATCATGCGTTGCGAAATCTAGCTATAGAGTTAACAGTTCAGCTTCGTAATTCAG
CAACGCTTGACTGGCAGAAGCGTGACAGTGTACGTGCTCGTATGCGTAACTTAGTCTGTCGACTGTTACGCCGCCGCCCG
TATCCACTTGAGACAATGTTCCGCATTCATTGTATGCAGCAATGGTACAGTCTTGGTGACGAAGCGACCGAAGATGCGTT
ATATGAAATTGCTTCAATGCGTCAGTTTGCACGATTATCACTGGATAAAGCGATCCCAGACCGCACCTCCATCATGAACT
TTAGGCACATGCTGGAAAAGCACAAACTCACGCGTAAATCATTCAAAACCGTTAACCAATGGCTGTCGGACTGCGGCGTG
ATGATGACGCAGGGAACCTTGGTTGATGCCACCATAATCGAAGCACCGAGCTCCACTAAGAATAAAAACAATGAGCGTGA
CCCAGATATGCATCAGACCAGGAAAGGTAACGAATGGTACTTCGGCATGAAAGCCCATATTGGCGTGGATGCCAACAGCG
GTCTCACCCATACGCTAGTCACCACTGCCGCCAATGAACATGACCTGAACCAATTGAATAAGTTGCTGCACGGTGAAGAG
GAATTCGTTTCCGGCGATGCCGGTTATCAGGGGGCAGAGAAACGGGAAGAGCTGGAAGACGCTGATGTGGAATGGTTAAT
CGCCGAGCGCCCCAGCAAAGTTCGCGCATTGAAAAAGCATCCCCGTAAGAACAAAACTGCCATCAGGATTGAATACCTGA
AAGCCAGTATCCGTGCAAAAGTGGAACACCCATTTCGCATCATCAAGTGCCAGTTCGGGTTCATCAAAACGCGTTATAAG
GGATTGATGAAAAATGACTCACAGTTAGCCATGTTATTCGCTTTGGCAAACCTGTTTAAAGTAGACCAGATGTTACGACG
ACAGCCAAGATCTGTCTGA

Upstream 100 bases:

>100_bases
GTTCACTCAGACTGCATCCTGACAACGCTGAACAAGTACCATAACCGCAGCATCGAAACCGCTCTGGTCATCGAAGAACT
GATTCCAGTGAACAAAAGAG

Downstream 100 bases:

>100_bases
AATCCGGGAATAGCCCGGAATAGGGCCAAAATCCGGCCTAAAACGCCCATTAATTGGCCTAAATTCGAAATTTAGCGTTT
AAATTTTAGGCTGGTAAGAT

Product: transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MHEDKELFDKLNLSVYEIVFYRALVENEASVHELGYHALRNLAIELTVQLRNSATLDWQKRDSVRARMRNLVCRLLRRRP
YPLETMFRIHCMQQWYSLGDEATEDALYEIASMRQFARLSLDKAIPDRTSIMNFRHMLEKHKLTRKSFKTVNQWLSDCGV
MMTQGTLVDATIIEAPSSTKNKNNERDPDMHQTRKGNEWYFGMKAHIGVDANSGLTHTLVTTAANEHDLNQLNKLLHGEE
EFVSGDAGYQGAEKREELEDADVEWLIAERPSKVRALKKHPRKNKTAIRIEYLKASIRAKVEHPFRIIKCQFGFIKTRYK
GLMKNDSQLAMLFALANLFKVDQMLRRQPRSV

Sequences:

>Translated_352_residues
MHEDKELFDKLNLSVYEIVFYRALVENEASVHELGYHALRNLAIELTVQLRNSATLDWQKRDSVRARMRNLVCRLLRRRP
YPLETMFRIHCMQQWYSLGDEATEDALYEIASMRQFARLSLDKAIPDRTSIMNFRHMLEKHKLTRKSFKTVNQWLSDCGV
MMTQGTLVDATIIEAPSSTKNKNNERDPDMHQTRKGNEWYFGMKAHIGVDANSGLTHTLVTTAANEHDLNQLNKLLHGEE
EFVSGDAGYQGAEKREELEDADVEWLIAERPSKVRALKKHPRKNKTAIRIEYLKASIRAKVEHPFRIIKCQFGFIKTRYK
GLMKNDSQLAMLFALANLFKVDQMLRRQPRSV
>Mature_352_residues
MHEDKELFDKLNLSVYEIVFYRALVENEASVHELGYHALRNLAIELTVQLRNSATLDWQKRDSVRARMRNLVCRLLRRRP
YPLETMFRIHCMQQWYSLGDEATEDALYEIASMRQFARLSLDKAIPDRTSIMNFRHMLEKHKLTRKSFKTVNQWLSDCGV
MMTQGTLVDATIIEAPSSTKNKNNERDPDMHQTRKGNEWYFGMKAHIGVDANSGLTHTLVTTAANEHDLNQLNKLLHGEE
EFVSGDAGYQGAEKREELEDADVEWLIAERPSKVRALKKHPRKNKTAIRIEYLKASIRAKVEHPFRIIKCQFGFIKTRYK
GLMKNDSQLAMLFALANLFKVDQMLRRQPRSV

Specific function: Involved in the transposition of the insertion sequence IS5 [H]

COG id: COG3039

COG function: function code L; Transposase and inactivated derivatives, IS5 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

Organism=Escherichia coli, GI1788303, Length=274, Percent_Identity=79.1970802919708, Blast_Score=474, Evalue=1e-135,
Organism=Escherichia coli, GI1789921, Length=274, Percent_Identity=78.8321167883212, Blast_Score=473, Evalue=1e-134,
Organism=Escherichia coli, GI1789612, Length=274, Percent_Identity=78.8321167883212, Blast_Score=473, Evalue=1e-134,
Organism=Escherichia coli, GI1789355, Length=274, Percent_Identity=78.8321167883212, Blast_Score=473, Evalue=1e-134,
Organism=Escherichia coli, GI1788518, Length=274, Percent_Identity=78.8321167883212, Blast_Score=473, Evalue=1e-134,
Organism=Escherichia coli, GI1788342, Length=274, Percent_Identity=78.8321167883212, Blast_Score=473, Evalue=1e-134,
Organism=Escherichia coli, GI1787592, Length=274, Percent_Identity=78.8321167883212, Blast_Score=473, Evalue=1e-134,
Organism=Escherichia coli, GI1786877, Length=274, Percent_Identity=78.8321167883212, Blast_Score=473, Evalue=1e-134,
Organism=Escherichia coli, GI1786764, Length=274, Percent_Identity=78.8321167883212, Blast_Score=473, Evalue=1e-134,
Organism=Escherichia coli, GI1786453, Length=274, Percent_Identity=78.8321167883212, Blast_Score=473, Evalue=1e-134,
Organism=Escherichia coli, GI1787634, Length=274, Percent_Identity=78.4671532846715, Blast_Score=471, Evalue=1e-134,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002559
- InterPro:   IPR008490 [H]

Pfam domain/function: PF05598 DUF772; PF01609 Transposase_11 [H]

EC number: NA

Molecular weight: Translated: 41011; Mature: 41011

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHEDKELFDKLNLSVYEIVFYRALVENEASVHELGYHALRNLAIELTVQLRNSATLDWQK
CCCHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHH
RDSVRARMRNLVCRLLRRRPYPLETMFRIHCMQQWYSLGDEATEDALYEIASMRQFARLS
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LDKAIPDRTSIMNFRHMLEKHKLTRKSFKTVNQWLSDCGVMMTQGTLVDATIIEAPSSTK
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEHHEEECCCCCC
NKNNERDPDMHQTRKGNEWYFGMKAHIGVDANSGLTHTLVTTAANEHDLNQLNKLLHGEE
CCCCCCCCCHHHHCCCCCEEEEEEEEEECCCCCCCCCHHEEECCCCCHHHHHHHHHCCCH
EFVSGDAGYQGAEKREELEDADVEWLIAERPSKVRALKKHPRKNKTAIRIEYLKASIRAK
HHHCCCCCCCCCHHHHHHCCCCCEEEECCCCHHHHHHHHCCCCCCCEEEHHHHHHHHHHH
VEHPFRIIKCQFGFIKTRYKGLMKNDSQLAMLFALANLFKVDQMLRRQPRSV
HCCCEEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MHEDKELFDKLNLSVYEIVFYRALVENEASVHELGYHALRNLAIELTVQLRNSATLDWQK
CCCHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHH
RDSVRARMRNLVCRLLRRRPYPLETMFRIHCMQQWYSLGDEATEDALYEIASMRQFARLS
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LDKAIPDRTSIMNFRHMLEKHKLTRKSFKTVNQWLSDCGVMMTQGTLVDATIIEAPSSTK
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEHHEEECCCCCC
NKNNERDPDMHQTRKGNEWYFGMKAHIGVDANSGLTHTLVTTAANEHDLNQLNKLLHGEE
CCCCCCCCCHHHHCCCCCEEEEEEEEEECCCCCCCCCHHEEECCCCCHHHHHHHHHCCCH
EFVSGDAGYQGAEKREELEDADVEWLIAERPSKVRALKKHPRKNKTAIRIEYLKASIRAK
HHHCCCCCCCCCHHHHHHCCCCCEEEECCCCHHHHHHHHCCCCCCCEEEHHHHHHHHHHH
VEHPFRIIKCQFGFIKTRYKGLMKNDSQLAMLFALANLFKVDQMLRRQPRSV
HCCCEEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8041620; 9097039; 9097040; 9278503 [H]