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The map label for this gene is ilvI [H]

Identifier: 37678679

GI number: 37678679

Start: 480810

End: 482534

Strand: Direct

Name: ilvI [H]

Synonym: VV0495

Alternate gene names: 37678679

Gene position: 480810-482534 (Clockwise)

Preceding gene: 37678678

Following gene: 37678680

Centisome position: 14.33

GC content: 47.65

Gene sequence:

>1725_bases
ATGACAGCAATGTTGTCAGGCGCAGAGATGGTTGTTCAATCTCTGATCGAAGAAAACGTAGAGCAAATTTTTGGTTATCC
CGGTGGTTCAGTACTGGATATTTATGATGCTCTTCACGCCAAAACTGAACAAATCAAGCATGTATTAGTTCGTCATGAAC
AAGCCGCGACCCACATGGCTGATGGTTATGCTCGTGCCACAGGCAAACCTGGCGTTGTCCTAGTCTGTTCAGGACCTGGC
GCAACGAACACCATCACCGGAATTGCCACGGCTTACATGGATTCCATCCCGATGATCGTTATTTCAGGAAACGTACCCAA
TAGCTTAATTGGTAATGATGCTTTCCAAGAGTGCGATATCGTTGGTGTTTCTCGCCCTGTGGTTAAACATAGTTTCCTCG
TTAAGCGTGCGGAAGACATTCCAGAGACCATCAAGAAAGCATTTTACATTGCCACGACAGGTCGCCCTGGCCCTGTGGTG
ATCGACTTGCCAAAAGATGTGATGAACCCGCTGATCAAACTGCCTTATGAATATCCTAAGTCAATCAGCATGCGGTCATA
CAAACCAACCACTTCGGGCCATAAAGGCCAAATCAAGAAAGCGCTAAAGGCATTACTTGAAGCGAAGAAGCCCGTTCTTT
ATATCGGTGGTGGAGCGATTATCTCAGGTGCGCATGAGTCGATCCTCAAGCTGGCTGAAACATTAAATTTGCCCGTTGTA
AGCACACTAATGGGGCTCGGTGCTTTCCCGGGGACCCACAAAAACGCTCTAGGCATGCTCGGCATGCATGGTAAATATGA
AGCCAATATGGCGATGCATAACGCCGATCTTATTTTTGGTGTTGGGGTACGTTTTGATGACCGTACCACCAACAACTTAG
AAAAATACTGCCCTAATGCGAAGATCATGCACATCGATATCGACCCTTCTTCGATCTCGAAGAATGTCAAAGTTGACCTA
CCCATCGTGGGGTCAGCGGAGCAAGTGCTTGAGACCATGGTGACACTTCTTGAAGAGCAAGGCGCTGAAAACGATACGCA
AGCCTTAGCGAGCTGGTGGGATGAAATTGAAGGCTGGAAAGCACGAGATTGCCTCGCCTACGACACCTCTTCGGAGCGCA
TCAAGCCACAACAAGTGATCGAGACACTGCATAAGCTGACCAATGGGGATGCTTATGTTGCGTCAGATGTAGGCCAGCAT
CAAATGTTTGCCGCGCTTTACTACCCGTTCAACAAACCGCGCCGTTGGATCAATTCTGGTGGTCTAGGCACGATGGGCTT
TGGTTTGCCCGCTGGTATGGGGGTGAAATTCGCTATGCCAGAAGAAGAAGTGGTGGTGGTGACGGGGGATGGCAGTATCC
AAATGAACATTCAAGAACTGTCGACTGCCATGCAATATGACATTCCGGTCAAAATTATCAACTTGAATAACCGTTTCCTA
GGGATGGTGAAACAGTGGCAAGACATTATCTATCAAGGTCGCCACTCTAACTCATACATGAGTTCTGTTCCTGATTTTGC
AGCCATTGCAGAAGCTTATGGCCATGTAGGTATTCGCATTGAAACCCCCGATCAACTCGAGTCAGAGCTTAAACGCGCCC
TCGACATGAAAGATCGCTTGGTGTTTGTCGATATCAATGTGGATGAAACTGAGCACGTCTACCCAATGCAAATCAAAGGC
GAAGGCATGGACAAAATGTGGCTAAGCAAAACGGAGAGAACTTAA

Upstream 100 bases:

>100_bases
TGAAGTTTTTTCGAATTAGGCTACAAATAAGCCCTAAACTATGTAAAACCAGCCCATTAGATTGACCTTATTTAATGGAA
ACTGGCTAAGGAGAAGCACT

Downstream 100 bases:

>100_bases
TATGAGACACATTATTTCGTTGTTAATGGAAAACCAACCGGGTGCCTTGTCTCGTGTGGTAGGGCTGTTCTCTCAGCGTG
GCTATAACATTGAATCGTTG

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 574; Mature: 573

Protein sequence:

>574_residues
MTAMLSGAEMVVQSLIEENVEQIFGYPGGSVLDIYDALHAKTEQIKHVLVRHEQAATHMADGYARATGKPGVVLVCSGPG
ATNTITGIATAYMDSIPMIVISGNVPNSLIGNDAFQECDIVGVSRPVVKHSFLVKRAEDIPETIKKAFYIATTGRPGPVV
IDLPKDVMNPLIKLPYEYPKSISMRSYKPTTSGHKGQIKKALKALLEAKKPVLYIGGGAIISGAHESILKLAETLNLPVV
STLMGLGAFPGTHKNALGMLGMHGKYEANMAMHNADLIFGVGVRFDDRTTNNLEKYCPNAKIMHIDIDPSSISKNVKVDL
PIVGSAEQVLETMVTLLEEQGAENDTQALASWWDEIEGWKARDCLAYDTSSERIKPQQVIETLHKLTNGDAYVASDVGQH
QMFAALYYPFNKPRRWINSGGLGTMGFGLPAGMGVKFAMPEEEVVVVTGDGSIQMNIQELSTAMQYDIPVKIINLNNRFL
GMVKQWQDIIYQGRHSNSYMSSVPDFAAIAEAYGHVGIRIETPDQLESELKRALDMKDRLVFVDINVDETEHVYPMQIKG
EGMDKMWLSKTERT

Sequences:

>Translated_574_residues
MTAMLSGAEMVVQSLIEENVEQIFGYPGGSVLDIYDALHAKTEQIKHVLVRHEQAATHMADGYARATGKPGVVLVCSGPG
ATNTITGIATAYMDSIPMIVISGNVPNSLIGNDAFQECDIVGVSRPVVKHSFLVKRAEDIPETIKKAFYIATTGRPGPVV
IDLPKDVMNPLIKLPYEYPKSISMRSYKPTTSGHKGQIKKALKALLEAKKPVLYIGGGAIISGAHESILKLAETLNLPVV
STLMGLGAFPGTHKNALGMLGMHGKYEANMAMHNADLIFGVGVRFDDRTTNNLEKYCPNAKIMHIDIDPSSISKNVKVDL
PIVGSAEQVLETMVTLLEEQGAENDTQALASWWDEIEGWKARDCLAYDTSSERIKPQQVIETLHKLTNGDAYVASDVGQH
QMFAALYYPFNKPRRWINSGGLGTMGFGLPAGMGVKFAMPEEEVVVVTGDGSIQMNIQELSTAMQYDIPVKIINLNNRFL
GMVKQWQDIIYQGRHSNSYMSSVPDFAAIAEAYGHVGIRIETPDQLESELKRALDMKDRLVFVDINVDETEHVYPMQIKG
EGMDKMWLSKTERT
>Mature_573_residues
TAMLSGAEMVVQSLIEENVEQIFGYPGGSVLDIYDALHAKTEQIKHVLVRHEQAATHMADGYARATGKPGVVLVCSGPGA
TNTITGIATAYMDSIPMIVISGNVPNSLIGNDAFQECDIVGVSRPVVKHSFLVKRAEDIPETIKKAFYIATTGRPGPVVI
DLPKDVMNPLIKLPYEYPKSISMRSYKPTTSGHKGQIKKALKALLEAKKPVLYIGGGAIISGAHESILKLAETLNLPVVS
TLMGLGAFPGTHKNALGMLGMHGKYEANMAMHNADLIFGVGVRFDDRTTNNLEKYCPNAKIMHIDIDPSSISKNVKVDLP
IVGSAEQVLETMVTLLEEQGAENDTQALASWWDEIEGWKARDCLAYDTSSERIKPQQVIETLHKLTNGDAYVASDVGQHQ
MFAALYYPFNKPRRWINSGGLGTMGFGLPAGMGVKFAMPEEEVVVVTGDGSIQMNIQELSTAMQYDIPVKIINLNNRFLG
MVKQWQDIIYQGRHSNSYMSSVPDFAAIAEAYGHVGIRIETPDQLESELKRALDMKDRLVFVDINVDETEHVYPMQIKGE
GMDKMWLSKTERT

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=599, Percent_Identity=26.7111853088481, Blast_Score=201, Evalue=2e-51,
Organism=Homo sapiens, GI21361361, Length=507, Percent_Identity=25.6410256410256, Blast_Score=149, Evalue=6e-36,
Organism=Escherichia coli, GI87081685, Length=573, Percent_Identity=72.4258289703316, Blast_Score=897, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=563, Percent_Identity=40.1420959147425, Blast_Score=423, Evalue=1e-119,
Organism=Escherichia coli, GI1786717, Length=542, Percent_Identity=33.5793357933579, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI1787096, Length=559, Percent_Identity=29.695885509839, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1788716, Length=574, Percent_Identity=24.390243902439, Blast_Score=176, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI17531299, Length=548, Percent_Identity=26.2773722627737, Blast_Score=170, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17531301, Length=548, Percent_Identity=26.2773722627737, Blast_Score=170, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17542570, Length=524, Percent_Identity=27.0992366412214, Blast_Score=154, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6323755, Length=582, Percent_Identity=41.9243986254296, Blast_Score=449, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6320816, Length=555, Percent_Identity=24.6846846846847, Blast_Score=127, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6323163, Length=488, Percent_Identity=22.9508196721311, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI19922626, Length=599, Percent_Identity=25.2086811352254, Blast_Score=182, Evalue=4e-46,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62988; Mature: 62857

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAMLSGAEMVVQSLIEENVEQIFGYPGGSVLDIYDALHAKTEQIKHVLVRHEQAATHMA
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DGYARATGKPGVVLVCSGPGATNTITGIATAYMDSIPMIVISGNVPNSLIGNDAFQECDI
CCHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHCCCHHHHCCE
VGVSRPVVKHSFLVKRAEDIPETIKKAFYIATTGRPGPVVIDLPKDVMNPLIKLPYEYPK
EECCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCEEEECCHHHHHHHHHCCCCCCC
SISMRSYKPTTSGHKGQIKKALKALLEAKKPVLYIGGGAIISGAHESILKLAETLNLPVV
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCEECCHHHHHHHHHHHCCCHHH
STLMGLGAFPGTHKNALGMLGMHGKYEANMAMHNADLIFGVGVRFDDRTTNNLEKYCPNA
HHHHHCCCCCCCCCCCHHCCCCCCCEECCEEEECCCEEEEECEEECCCCCCCHHHHCCCC
KIMHIDIDPSSISKNVKVDLPIVGSAEQVLETMVTLLEEQGAENDTQALASWWDEIEGWK
EEEEEECCHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
ARDCLAYDTSSERIKPQQVIETLHKLTNGDAYVASDVGQHQMFAALYYPFNKPRRWINSG
CCCEEEECCCCCCCCHHHHHHHHHHHCCCCEEEECCCCHHHEEEEEECCCCCCHHHHCCC
GLGTMGFGLPAGMGVKFAMPEEEVVVVTGDGSIQMNIQELSTAMQYDIPVKIINLNNRFL
CCCCCCCCCCCCCCEEEECCCCCEEEEECCCCEEEEHHHHHHHHHCCCCEEEEECCCHHH
GMVKQWQDIIYQGRHSNSYMSSVPDFAAIAEAYGHVGIRIETPDQLESELKRALDMKDRL
HHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHCCEEEEECCHHHHHHHHHHHHCCCCCE
VFVDINVDETEHVYPMQIKGEGMDKMWLSKTERT
EEEEECCCCCCCEEEEEECCCCCCHHHHHCCCCC
>Mature Secondary Structure 
TAMLSGAEMVVQSLIEENVEQIFGYPGGSVLDIYDALHAKTEQIKHVLVRHEQAATHMA
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DGYARATGKPGVVLVCSGPGATNTITGIATAYMDSIPMIVISGNVPNSLIGNDAFQECDI
CCHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHCCCHHHHCCE
VGVSRPVVKHSFLVKRAEDIPETIKKAFYIATTGRPGPVVIDLPKDVMNPLIKLPYEYPK
EECCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCEEEECCHHHHHHHHHCCCCCCC
SISMRSYKPTTSGHKGQIKKALKALLEAKKPVLYIGGGAIISGAHESILKLAETLNLPVV
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCEECCHHHHHHHHHHHCCCHHH
STLMGLGAFPGTHKNALGMLGMHGKYEANMAMHNADLIFGVGVRFDDRTTNNLEKYCPNA
HHHHHCCCCCCCCCCCHHCCCCCCCEECCEEEECCCEEEEECEEECCCCCCCHHHHCCCC
KIMHIDIDPSSISKNVKVDLPIVGSAEQVLETMVTLLEEQGAENDTQALASWWDEIEGWK
EEEEEECCHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
ARDCLAYDTSSERIKPQQVIETLHKLTNGDAYVASDVGQHQMFAALYYPFNKPRRWINSG
CCCEEEECCCCCCCCHHHHHHHHHHHCCCCEEEECCCCHHHEEEEEECCCCCCHHHHCCC
GLGTMGFGLPAGMGVKFAMPEEEVVVVTGDGSIQMNIQELSTAMQYDIPVKIINLNNRFL
CCCCCCCCCCCCCCEEEECCCCCEEEEECCCCEEEEHHHHHHHHHCCCCEEEEECCCHHH
GMVKQWQDIIYQGRHSNSYMSSVPDFAAIAEAYGHVGIRIETPDQLESELKRALDMKDRL
HHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHCCEEEEECCHHHHHHHHHHHHCCCCCE
VFVDINVDETEHVYPMQIKGEGMDKMWLSKTERT
EEEEECCCCCCCEEEEEECCCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]