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The map label for this gene is pyrE

Identifier: 37678464

GI number: 37678464

Start: 291084

End: 291725

Strand: Direct

Name: pyrE

Synonym: VV0280

Alternate gene names: 37678464

Gene position: 291084-291725 (Clockwise)

Preceding gene: 37678463

Following gene: 37678468

Centisome position: 8.68

GC content: 49.38

Gene sequence:

>642_bases
ATGAAAGCATACCAGCGTGAATTTATTGAGTTTGCACTAGAGAAACAAGTCCTTAAGTTTGGCGAGTTTACGTTGAAATC
TGGCCGTAAAAGCCCATATTTCTTTAACGCTGGTCTTTTCAATACAGGTCGTGACTTGGCACGCCTTGGTCGTTTTTATG
CGGCGGCGTTAGCGGATTCTGGCATTGAGTTCGACGTACTCTTTGGCCCTGCATACAAAGGTATCCCAATCGCGACTACG
ACAGCGGTTGCACTTGCCGATCACCATGATATCGACACGCCTTACTGCTTCAATCGCAAAGAAGCCAAAGATCACGGCGA
AGGCGGTAACTTGGTTGGCTCTGCGCTAGAAGGGCGCATTATGCTGGTGGACGACGTGATCACCGCGGGCACAGCAATTC
GCGAATCGATGGAAATCATCCAAGCTAATGGTGCCGATCTGGCTGGTGTATTGGTTGCGATTGACCGCCAAGAAAAAGGT
AAGGGTGAGCTGTCGGCGATTCAGGAAGTTGAACGTGATTTCGCTTGTGCGGTGATTTCTATCGTTAGCCTGTCGGATTT
GATCACTTTCTTGGAAGAGAAAGGCGATACAGCCGAGCATCTTGATGCGGTGAAAGCGTATCGCGCCCAATACGGGATCT
AA

Upstream 100 bases:

>100_bases
TAGACCCTAGACCCTAGACCCTAGACCCTAGACCCTAGACCCTAGACCCTAGACCCTAGACCTAGCACCTATTAACCCAT
TAAACATCGAGAGAGTATTC

Downstream 100 bases:

>100_bases
GTAAAGGCAGGGGCTAGATTCTAGGAAAAGCAGCTCCTAGAATCTAGTTATCTAGGCTTCTAGTCCCTAGTCCCTAGTCC
CTAGCAACCTAACACCTAAC

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MKAYQREFIEFALEKQVLKFGEFTLKSGRKSPYFFNAGLFNTGRDLARLGRFYAAALADSGIEFDVLFGPAYKGIPIATT
TAVALADHHDIDTPYCFNRKEAKDHGEGGNLVGSALEGRIMLVDDVITAGTAIRESMEIIQANGADLAGVLVAIDRQEKG
KGELSAIQEVERDFACAVISIVSLSDLITFLEEKGDTAEHLDAVKAYRAQYGI

Sequences:

>Translated_213_residues
MKAYQREFIEFALEKQVLKFGEFTLKSGRKSPYFFNAGLFNTGRDLARLGRFYAAALADSGIEFDVLFGPAYKGIPIATT
TAVALADHHDIDTPYCFNRKEAKDHGEGGNLVGSALEGRIMLVDDVITAGTAIRESMEIIQANGADLAGVLVAIDRQEKG
KGELSAIQEVERDFACAVISIVSLSDLITFLEEKGDTAEHLDAVKAYRAQYGI
>Mature_213_residues
MKAYQREFIEFALEKQVLKFGEFTLKSGRKSPYFFNAGLFNTGRDLARLGRFYAAALADSGIEFDVLFGPAYKGIPIATT
TAVALADHHDIDTPYCFNRKEAKDHGEGGNLVGSALEGRIMLVDDVITAGTAIRESMEIIQANGADLAGVLVAIDRQEKG
KGELSAIQEVERDFACAVISIVSLSDLITFLEEKGDTAEHLDAVKAYRAQYGI

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=195, Percent_Identity=29.2307692307692, Blast_Score=89, Evalue=3e-18,
Organism=Escherichia coli, GI1790073, Length=213, Percent_Identity=83.5680751173709, Blast_Score=377, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17508631, Length=163, Percent_Identity=27.6073619631902, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17554964, Length=195, Percent_Identity=25.1282051282051, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323530, Length=220, Percent_Identity=51.3636363636364, Blast_Score=209, Evalue=3e-55,
Organism=Saccharomyces cerevisiae, GI6323927, Length=221, Percent_Identity=46.1538461538462, Blast_Score=194, Evalue=9e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 23204; Mature: 23204

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAYQREFIEFALEKQVLKFGEFTLKSGRKSPYFFNAGLFNTGRDLARLGRFYAAALADS
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCC
GIEFDVLFGPAYKGIPIATTTAVALADHHDIDTPYCFNRKEAKDHGEGGNLVGSALEGRI
CCEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCEEHHHHCCCE
MLVDDVITAGTAIRESMEIIQANGADLAGVLVAIDRQEKGKGELSAIQEVERDFACAVIS
EEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
IVSLSDLITFLEEKGDTAEHLDAVKAYRAQYGI
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKAYQREFIEFALEKQVLKFGEFTLKSGRKSPYFFNAGLFNTGRDLARLGRFYAAALADS
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCC
GIEFDVLFGPAYKGIPIATTTAVALADHHDIDTPYCFNRKEAKDHGEGGNLVGSALEGRI
CCEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCEEHHHHCCCE
MLVDDVITAGTAIRESMEIIQANGADLAGVLVAIDRQEKGKGELSAIQEVERDFACAVIS
EEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
IVSLSDLITFLEEKGDTAEHLDAVKAYRAQYGI
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]