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The map label for this gene is rph

Identifier: 37678463

GI number: 37678463

Start: 290211

End: 290927

Strand: Direct

Name: rph

Synonym: VV0279

Alternate gene names: 37678463

Gene position: 290211-290927 (Clockwise)

Preceding gene: 37678458

Following gene: 37678464

Centisome position: 8.65

GC content: 54.39

Gene sequence:

>717_bases
ATGCGTCCAAATGATCGCGCGGCAGACCAAGTGCGCCCAATTAAAATTACTCGTAACTACACGGCATATGCGGAAGGTTC
CGTGCTGGTTGAGTTTGGTAATACCAAGGTGCTGTGTAATGCGACCGTGGAAGAATCGGTGCCTCGTTGGCTAAAAGGCC
AAGGTAAAGGCTGGGTGACGGCGGAATACGGCATGCTGCCACGCGCGACCCACTCACGTACTCGTCGTGAAGCGGCCAAT
GGCAAGCAAGGCGGCCGTACCATGGAGATTCAACGCTTGATCGCACGTAGCTTGCGCGCAGTGGTGGATCTGGAAGCGAT
GGGCGAGTTTATGATCACCGTCGACTGCGATGTGATCCAAGCCGATGGTGGCACACGCACTGCGTCAATCAGTGGCGCTA
GCGTTGCGATGGCGGATGCATTCCAACACCTTGTTGACAGTGGCAAGCTAAAAGCAAACCCAATGAAAGGCCATGTGGCT
GCGGTTTCGGTCGGCATTTTGGGTGAAGATGTACTGTGCGATTTAGAATACGTGGAAGATTCGGCGGCGGATACCGACAT
GAACGTAGTGATGACGGAAGAAGGCAAGATGATCGAAATCCAAGGCACTGCCGAAGGCGAACCGTTTAGCCATGAACAAT
TGCTGGCTCTGCTAGAGTCGGCCAAAAAGGGCATTAGCGAGATCGTCGCCGCGCAGAAGGCGGCGTTAGCCAATTGA

Upstream 100 bases:

>100_bases
ACTGTGAGCTAAAAACCGTGAGCCTTTCCCTAGTGCCTTGCGGTGGTTTCCGCTATACTTCACCCCCAATCAAATCGATC
CCCAGAAAAGGTATTCATCC

Downstream 100 bases:

>100_bases
TTTAGATAGCTCCCATTTGGGGGCTATTTTTTTGTTTGTTGCTAGACCCTAGACCCTAGACCCTAGACCCTAGACCCTAG
ACCCTAGACCCTAGACCCTA

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MRPNDRAADQVRPIKITRNYTAYAEGSVLVEFGNTKVLCNATVEESVPRWLKGQGKGWVTAEYGMLPRATHSRTRREAAN
GKQGGRTMEIQRLIARSLRAVVDLEAMGEFMITVDCDVIQADGGTRTASISGASVAMADAFQHLVDSGKLKANPMKGHVA
AVSVGILGEDVLCDLEYVEDSAADTDMNVVMTEEGKMIEIQGTAEGEPFSHEQLLALLESAKKGISEIVAAQKAALAN

Sequences:

>Translated_238_residues
MRPNDRAADQVRPIKITRNYTAYAEGSVLVEFGNTKVLCNATVEESVPRWLKGQGKGWVTAEYGMLPRATHSRTRREAAN
GKQGGRTMEIQRLIARSLRAVVDLEAMGEFMITVDCDVIQADGGTRTASISGASVAMADAFQHLVDSGKLKANPMKGHVA
AVSVGILGEDVLCDLEYVEDSAADTDMNVVMTEEGKMIEIQGTAEGEPFSHEQLLALLESAKKGISEIVAAQKAALAN
>Mature_238_residues
MRPNDRAADQVRPIKITRNYTAYAEGSVLVEFGNTKVLCNATVEESVPRWLKGQGKGWVTAEYGMLPRATHSRTRREAAN
GKQGGRTMEIQRLIARSLRAVVDLEAMGEFMITVDCDVIQADGGTRTASISGASVAMADAFQHLVDSGKLKANPMKGHVA
AVSVGILGEDVLCDLEYVEDSAADTDMNVVMTEEGKMIEIQGTAEGEPFSHEQLLALLESAKKGISEIVAAQKAALAN

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=211, Percent_Identity=75.3554502369668, Blast_Score=348, Evalue=3e-97,
Organism=Caenorhabditis elegans, GI71981632, Length=181, Percent_Identity=27.6243093922652, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 25566; Mature: 25566

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPNDRAADQVRPIKITRNYTAYAEGSVLVEFGNTKVLCNATVEESVPRWLKGQGKGWVT
CCCCCCCHHCEEEEEEECCEEEEECCEEEEEECCEEEEEECCHHHHCCHHHCCCCCEEEE
AEYGMLPRATHSRTRREAANGKQGGRTMEIQRLIARSLRAVVDLEAMGEFMITVDCDVIQ
EECCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEE
ADGGTRTASISGASVAMADAFQHLVDSGKLKANPMKGHVAAVSVGILGEDVLCDLEYVED
CCCCCEEEECCCCHHHHHHHHHHHHHCCCEECCCCCCEEEEEEEEECCCCCEEEHHHHCC
SAADTDMNVVMTEEGKMIEIQGTAEGEPFSHEQLLALLESAKKGISEIVAAQKAALAN
CCCCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRPNDRAADQVRPIKITRNYTAYAEGSVLVEFGNTKVLCNATVEESVPRWLKGQGKGWVT
CCCCCCCHHCEEEEEEECCEEEEECCEEEEEECCEEEEEECCHHHHCCHHHCCCCCEEEE
AEYGMLPRATHSRTRREAANGKQGGRTMEIQRLIARSLRAVVDLEAMGEFMITVDCDVIQ
EECCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEE
ADGGTRTASISGASVAMADAFQHLVDSGKLKANPMKGHVAAVSVGILGEDVLCDLEYVED
CCCCCEEEECCCCHHHHHHHHHHHHHCCCEECCCCCCEEEEEEEEECCCCCEEEHHHHCC
SAADTDMNVVMTEEGKMIEIQGTAEGEPFSHEQLLALLESAKKGISEIVAAQKAALAN
CCCCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]