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The map label for this gene is lipB

Identifier: 37523983

GI number: 37523983

Start: 4642926

End: 4643594

Strand: Reverse

Name: lipB

Synonym: gll4414

Alternate gene names: 37523983

Gene position: 4643594-4642926 (Counterclockwise)

Preceding gene: 37523984

Following gene: 37523981

Centisome position: 99.67

GC content: 65.47

Gene sequence:

>669_bases
ATGGGGCGTTGCCTGCTCATCGAGCCTGGGCTGGTTCCCTACCTAACCGCCTGGGAGTGGCAGCGGCAACTGATGGCGAC
GCGCATCGCCCATCGCGACCGGCCGGACGTGCTGATGCTGCTGGAACACCCGCCGGTCTACACCCTCGGCCAGGGCGCCG
ACGCCAAACATGTGCTCGTGGATCCGGCGAGCATCGAACTGTACCGCACCGAGCGCGGCGGTGAAGTCACTTACCACGGC
CCGGGGCAACTGGTGGGCTACCCGATTCTCGATCTGACCGGCTACCGCCAGGATTTGCACTGGTATTTGCGCACCCTCGA
ACAGGTACTCATCGAGGTTCTAGCCGATTTTGGGGTACAGGGCGAGCGAGAGGCGGGCTTTACGGGAGTGTGGGCAGGCG
GCCGGAAAATCGCCGCCCTCGGCATCAAGGTCTCGCGCTGGGTGACGATGCACGGCTTCGCCCTCAACGTGGACCCGGAT
CTGGATGCTTTTGCCCGGATCGTCCCCTGCGGCCTGACACGGCCGGTGGGCTCGCTTGTTCAGCTGTGTCCGGGAGTCAG
CGTAGAGCAGGTGCAACCGGTGGTGGCGTGCGCCTTTGCCCGCGTCTTCGGGGTACAGTGCGAACCGGGTGCTCTTGAAG
CGTGCCTCGCCGTCAAGCCGGGCTGCTGA

Upstream 100 bases:

>100_bases
GGATAACCGATCCGAACTGCGGATTTGCCGAGGCGGCGGAACTGACGGGGGCGGTATTGCAGGTGGGCAAAAAGCACTTT
CGCCGCCTGGTGCCGAGCTG

Downstream 100 bases:

>100_bases
ACTGCGGTTCGACGCCGATAATGCGGGCGCTGCGGGCGTCCACGAGCACCGTCGAGCGGTCGAAGGCGACGGCGTAGGCG
AGAATCGCCAACTCCTCCGA

Product: lipoyltransferase

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MGRCLLIEPGLVPYLTAWEWQRQLMATRIAHRDRPDVLMLLEHPPVYTLGQGADAKHVLVDPASIELYRTERGGEVTYHG
PGQLVGYPILDLTGYRQDLHWYLRTLEQVLIEVLADFGVQGEREAGFTGVWAGGRKIAALGIKVSRWVTMHGFALNVDPD
LDAFARIVPCGLTRPVGSLVQLCPGVSVEQVQPVVACAFARVFGVQCEPGALEACLAVKPGC

Sequences:

>Translated_222_residues
MGRCLLIEPGLVPYLTAWEWQRQLMATRIAHRDRPDVLMLLEHPPVYTLGQGADAKHVLVDPASIELYRTERGGEVTYHG
PGQLVGYPILDLTGYRQDLHWYLRTLEQVLIEVLADFGVQGEREAGFTGVWAGGRKIAALGIKVSRWVTMHGFALNVDPD
LDAFARIVPCGLTRPVGSLVQLCPGVSVEQVQPVVACAFARVFGVQCEPGALEACLAVKPGC
>Mature_221_residues
GRCLLIEPGLVPYLTAWEWQRQLMATRIAHRDRPDVLMLLEHPPVYTLGQGADAKHVLVDPASIELYRTERGGEVTYHGP
GQLVGYPILDLTGYRQDLHWYLRTLEQVLIEVLADFGVQGEREAGFTGVWAGGRKIAALGIKVSRWVTMHGFALNVDPDL
DAFARIVPCGLTRPVGSLVQLCPGVSVEQVQPVVACAFARVFGVQCEPGALEACLAVKPGC

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=142, Percent_Identity=40.8450704225352, Blast_Score=85, Evalue=4e-17,
Organism=Escherichia coli, GI87081767, Length=192, Percent_Identity=35.4166666666667, Blast_Score=121, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6323268, Length=212, Percent_Identity=27.8301886792453, Blast_Score=79, Evalue=8e-16,
Organism=Drosophila melanogaster, GI21355483, Length=196, Percent_Identity=37.7551020408163, Blast_Score=123, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_GLOVI (Q7ND22)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_927360.1
- ProteinModelPortal:   Q7ND22
- SMR:   Q7ND22
- GeneID:   2602817
- GenomeReviews:   BA000045_GR
- KEGG:   gvi:gll4414
- NMPDR:   fig|251221.1.peg.4414
- HOGENOM:   HBG716160
- OMA:   DRGGSIT
- BioCyc:   GVIO251221:GLL4414-MONOMER
- BRENDA:   2.3.1.181
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 24299; Mature: 24167

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 170-170

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRCLLIEPGLVPYLTAWEWQRQLMATRIAHRDRPDVLMLLEHPPVYTLGQGADAKHVLV
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEECCCCCCCEEEE
DPASIELYRTERGGEVTYHGPGQLVGYPILDLTGYRQDLHWYLRTLEQVLIEVLADFGVQ
CCCEEEEEEECCCCEEEECCCCCEECCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCC
GEREAGFTGVWAGGRKIAALGIKVSRWVTMHGFALNVDPDLDAFARIVPCGLTRPVGSLV
CCCCCCCEEEECCCCEEEEEEEEEEEEEEEECEEEECCCCHHHHHHHHCCCCCCCHHHHH
QLCPGVSVEQVQPVVACAFARVFGVQCEPGALEACLAVKPGC
HHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHEEEECCCCC
>Mature Secondary Structure 
GRCLLIEPGLVPYLTAWEWQRQLMATRIAHRDRPDVLMLLEHPPVYTLGQGADAKHVLV
CCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEECCCCCCCEEEE
DPASIELYRTERGGEVTYHGPGQLVGYPILDLTGYRQDLHWYLRTLEQVLIEVLADFGVQ
CCCEEEEEEECCCCEEEECCCCCEECCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCC
GEREAGFTGVWAGGRKIAALGIKVSRWVTMHGFALNVDPDLDAFARIVPCGLTRPVGSLV
CCCCCCCEEEECCCCEEEEEEEEEEEEEEEECEEEECCCCHHHHHHHHCCCCCCCHHHHH
QLCPGVSVEQVQPVVACAFARVFGVQCEPGALEACLAVKPGC
HHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292