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The map label for this gene is tyrS

Identifier: 37523984

GI number: 37523984

Start: 4643594

End: 4644802

Strand: Reverse

Name: tyrS

Synonym: gvip571

Alternate gene names: 37523984

Gene position: 4644802-4643594 (Counterclockwise)

Preceding gene: 37523985

Following gene: 37523983

Centisome position: 99.69

GC content: 63.28

Gene sequence:

>1209_bases
ATGGACGAGCGGCAAGTGGAGCGCCTGAGTGGGCACGGCGTGGCAGAAATTTTCCCGGGCGGAGCGGAGGCGCTCACCCG
GCGCCTCGCGGATGCTGAAACCGCTAGAATGCCCCTGCGCGTCAAACTGGGGATCGATCCGACCCGGCCGGATCTGCACC
TCGGGCACACGGTCGTACTGCGCAAATTGCGCGAATTTCAAGATCTGGGCCACACCGCCATCTTGCTCATCGGCGGCTTT
ACCGCCCAGATAGGCGACCCGACCGGCAAGTCCGAAGCCCGCCCGCGCCTGAGTGCCGCTGAGGTCGAGGAGTACGCCGA
AACCTATCTCGACCAGGCCCGGCTGATTCTGAATTTCGACACACTCGAAGTGCGCAACAACACCGAGTGGCTATCGGGTC
TGGATCTGGCCGCAATTATCAAGCTCCTCGCCTCCACCAGCGTCGCCCAGATGCTCGCCAAAGAGGACTTTGGCCAGCGC
TACGAGGCGGGTACGGCCATCTACTTGCACGAATTTCTCTACCCGCTGATGCAAGGCTACGACTCGGTCGCCCTCGACGC
CGACATCGAACTGGGCGGGACCGACCAACGCTTTAACTTGCTCATGGGCCGCAACTTGCAAGAATTGTTCGGCAAAAAGC
CGCAGCTTGCCCTCACGGTGCCGTTGCTGGTGGGCCTCGACGGCGTCAAGAAAATGTCCAAATCCCTCGACAATTACGTG
GGCGTCACCGAAGACCCGCTCACGATGTACTCGAAGCTGGAGAAGACCCCCGACGCCCTGGTGGAAACGTACTTCGAGCT
GCTCACGGATTTGAGCGTCTCTGCCCTGTCCGCCAACCCCCGCGAGCGGCAAAAACGCCTGGCCCTCGCCGTCACCGGTT
GGCTGCACTCGCCCGAGCAGGCTCTGCGCGCCCAGGAAGCGGCGGCAGCCATGGTCACCGGCTCCGGCGGGACCGGTGCG
GATCTGGAGGCGGTGCCGGAGTTCTCCCTGACCGAGATCGCCTTTCCGGTGCGGCTTGCCAACTTGCTCAAAGCAGCCGG
GCTGTGCCCGACTGTCTCCGAAGGCATGCGCCAGATCAAAAACGGGGCGGTGCGCCTCAACCAGGAGCGGATAACCGATC
CGAACTGCGGATTTGCCGAGGCGGCGGAACTGACGGGGGCGGTATTGCAGGTGGGCAAAAAGCACTTTCGCCGCCTGGTG
CCGAGCTGA

Upstream 100 bases:

>100_bases
GCGATCAAAGTGGACGAAGCGGCCCTTGCCGAGGCGCGCCGCATTGCCGGCGAGACGGCCAAGCCCGTTAAATAGGTGGT
TGAACCGTTGCAAAACGACG

Downstream 100 bases:

>100_bases
TGGGGCGTTGCCTGCTCATCGAGCCTGGGCTGGTTCCCTACCTAACCGCCTGGGAGTGGCAGCGGCAACTGATGGCGACG
CGCATCGCCCATCGCGACCG

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MDERQVERLSGHGVAEIFPGGAEALTRRLADAETARMPLRVKLGIDPTRPDLHLGHTVVLRKLREFQDLGHTAILLIGGF
TAQIGDPTGKSEARPRLSAAEVEEYAETYLDQARLILNFDTLEVRNNTEWLSGLDLAAIIKLLASTSVAQMLAKEDFGQR
YEAGTAIYLHEFLYPLMQGYDSVALDADIELGGTDQRFNLLMGRNLQELFGKKPQLALTVPLLVGLDGVKKMSKSLDNYV
GVTEDPLTMYSKLEKTPDALVETYFELLTDLSVSALSANPRERQKRLALAVTGWLHSPEQALRAQEAAAAMVTGSGGTGA
DLEAVPEFSLTEIAFPVRLANLLKAAGLCPTVSEGMRQIKNGAVRLNQERITDPNCGFAEAAELTGAVLQVGKKHFRRLV
PS

Sequences:

>Translated_402_residues
MDERQVERLSGHGVAEIFPGGAEALTRRLADAETARMPLRVKLGIDPTRPDLHLGHTVVLRKLREFQDLGHTAILLIGGF
TAQIGDPTGKSEARPRLSAAEVEEYAETYLDQARLILNFDTLEVRNNTEWLSGLDLAAIIKLLASTSVAQMLAKEDFGQR
YEAGTAIYLHEFLYPLMQGYDSVALDADIELGGTDQRFNLLMGRNLQELFGKKPQLALTVPLLVGLDGVKKMSKSLDNYV
GVTEDPLTMYSKLEKTPDALVETYFELLTDLSVSALSANPRERQKRLALAVTGWLHSPEQALRAQEAAAAMVTGSGGTGA
DLEAVPEFSLTEIAFPVRLANLLKAAGLCPTVSEGMRQIKNGAVRLNQERITDPNCGFAEAAELTGAVLQVGKKHFRRLV
PS
>Mature_402_residues
MDERQVERLSGHGVAEIFPGGAEALTRRLADAETARMPLRVKLGIDPTRPDLHLGHTVVLRKLREFQDLGHTAILLIGGF
TAQIGDPTGKSEARPRLSAAEVEEYAETYLDQARLILNFDTLEVRNNTEWLSGLDLAAIIKLLASTSVAQMLAKEDFGQR
YEAGTAIYLHEFLYPLMQGYDSVALDADIELGGTDQRFNLLMGRNLQELFGKKPQLALTVPLLVGLDGVKKMSKSLDNYV
GVTEDPLTMYSKLEKTPDALVETYFELLTDLSVSALSANPRERQKRLALAVTGWLHSPEQALRAQEAAAAMVTGSGGTGA
DLEAVPEFSLTEIAFPVRLANLLKAAGLCPTVSEGMRQIKNGAVRLNQERITDPNCGFAEAAELTGAVLQVGKKHFRRLV
PS

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=410, Percent_Identity=26.0975609756098, Blast_Score=115, Evalue=7e-26,
Organism=Escherichia coli, GI1787925, Length=402, Percent_Identity=29.8507462686567, Blast_Score=144, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI25145802, Length=205, Percent_Identity=29.2682926829268, Blast_Score=82, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6325160, Length=393, Percent_Identity=26.2086513994911, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI20130383, Length=447, Percent_Identity=25.9507829977629, Blast_Score=116, Evalue=3e-26,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_GLOVI (Q7ND21)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_927361.1
- ProteinModelPortal:   Q7ND21
- SMR:   Q7ND21
- GeneID:   2602818
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.4415
- HOGENOM:   HBG288125
- OMA:   GHAVILR
- ProtClustDB:   PRK05912
- BioCyc:   GVIO251221:GLL4415-MONOMER
- BRENDA:   6.1.1.1
- GO:   GO:0005737
- HAMAP:   MF_02007
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 43865; Mature: 43865

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 234-234

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDERQVERLSGHGVAEIFPGGAEALTRRLADAETARMPLRVKLGIDPTRPDLHLGHTVVL
CCHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHH
RKLREFQDLGHTAILLIGGFTAQIGDPTGKSEARPRLSAAEVEEYAETYLDQARLILNFD
HHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEE
TLEVRNNTEWLSGLDLAAIIKLLASTSVAQMLAKEDFGQRYEAGTAIYLHEFLYPLMQGY
EEEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
DSVALDADIELGGTDQRFNLLMGRNLQELFGKKPQLALTVPLLVGLDGVKKMSKSLDNYV
CCEEEECCEEECCCCHHHHHHHCCCHHHHCCCCCCEEEEHHHHHCCHHHHHHHHHHHHHC
GVTEDPLTMYSKLEKTPDALVETYFELLTDLSVSALSANPRERQKRLALAVTGWLHSPEQ
CCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHCCCCHHH
ALRAQEAAAAMVTGSGGTGADLEAVPEFSLTEIAFPVRLANLLKAAGLCPTVSEGMRQIK
HHHHHHHHHEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
NGAVRLNQERITDPNCGFAEAAELTGAVLQVGKKHFRRLVPS
CCCEEECHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDERQVERLSGHGVAEIFPGGAEALTRRLADAETARMPLRVKLGIDPTRPDLHLGHTVVL
CCHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHH
RKLREFQDLGHTAILLIGGFTAQIGDPTGKSEARPRLSAAEVEEYAETYLDQARLILNFD
HHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEE
TLEVRNNTEWLSGLDLAAIIKLLASTSVAQMLAKEDFGQRYEAGTAIYLHEFLYPLMQGY
EEEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
DSVALDADIELGGTDQRFNLLMGRNLQELFGKKPQLALTVPLLVGLDGVKKMSKSLDNYV
CCEEEECCEEECCCCHHHHHHHCCCHHHHCCCCCCEEEEHHHHHCCHHHHHHHHHHHHHC
GVTEDPLTMYSKLEKTPDALVETYFELLTDLSVSALSANPRERQKRLALAVTGWLHSPEQ
CCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHCCCCHHH
ALRAQEAAAAMVTGSGGTGADLEAVPEFSLTEIAFPVRLANLLKAAGLCPTVSEGMRQIK
HHHHHHHHHEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
NGAVRLNQERITDPNCGFAEAAELTGAVLQVGKKHFRRLVPS
CCCEEECHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292