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The map label for this gene is purA

Identifier: 37522849

GI number: 37522849

Start: 3486281

End: 3487603

Strand: Direct

Name: purA

Synonym: gvip453

Alternate gene names: 37522849

Gene position: 3486281-3487603 (Clockwise)

Preceding gene: 37522848

Following gene: 37522850

Centisome position: 74.83

GC content: 63.64

Gene sequence:

>1323_bases
TTGGCTAACGTAATCGTGGTCGGCGCCCAGTGGGGCGACGAAGGAAAGGGCAAGATCACCGATCTGCTCAGTGTCTCGGC
GGATGTCGTCGTCCGCTACCAGGGAGGCATCAATGCCGGGCACACCGTTTGTGTCGGCGAGCAGACCTTCAAGTTGCATT
TGATTCCCTCGGGCATCCTCTACCCCGAGACGTGCTGTGTGATCGCCTCCGGCACGGTGGTCGATCCCAAGGTTCTACTC
GAAGAGATCCGGCACTTAGAAGAGCTGGGAATCTCGACAAAGAATTTGTTCATCGCCGAAACGGCCCACGTCACGATGCC
CTACCACCGGCTCATCGACATCGCCGAGGAGGAGCGCCGGGGCAAAAACCGCATCGGCACCACTGGCCGCGGCATCGGCC
CGACCTACGCCGACAAGTCCGGCCGCACGGGCTTTCGGGTGCTCGATTTGATGCAGCCGGAGGGCTTTCGCGAAAAACTC
GAATGGATCGTGCCCCTCAAGAACGTCTTGCTCGAGAGGCTCTACAACCTGCCGCCGCTCGCGGTCGATCAAGTGGCTGA
GCAGTACCTGGCCTACGCCGAGCAACTGAGGCCCTACGTTATCGACGCCTCGCTGCTGATTGATCAGGCGGTTCGCACCC
ACCGGAACATTCTCTTTGAGGGCGCCCAGGGCACCCTGCTCGATCTCGACCACGGCACCTACCCCTACGTCACTTCCTCC
AACCCGGTGGCGGGCGGCGCCTGCGTCGGGGCGGGGGTTGGACCCACGATCATCGACCGGGTGATCGGGGTGGCCAAGGC
CTACACCACCCGCGTCGGTGAAGGCCCGTTTCCGACCGAATTGCGCGACGAGGTCGGCCGCCACCTCGGGGAGCGCGGCG
CCGAATTCGGCACCACCACCGGTAGGCCGCGCCGCTGCGGCTGGTTCGACGCGGTGATCGGCCGCTACGCCGTGCGCATC
AACGGCCTCGACTGCCTGGCCATCACCAAGCTCGACGTGCTCGACGAACTGGAGAGCATCAAAGTCTGCGTCGCTTACCA
CTGCCCGAGCAAGACGGTCGAAGAATTTCCGAGCGACGCCAATCTGTTCGGTCGCTGCGAACCGGTCTACGAGACTCTGC
CCGGCTGGAAAGAACCGACCGGCCACTGCCGCACCCTCGAGGAGTTGCCGCCCAAGGCACGCGACTATCTCGAATTTCTC
GCCCACCAGATGAAGACGCCCATCGCCCTGGTCTCGGTCGGTGCCAACCGCGAGCAGACGATCATCGTCGAAGATCCGAT
CCACGGCCCCAAGCGCGGACTGCTCGGGCACAACGGCGATTAG

Upstream 100 bases:

>100_bases
GCGCGTGGTTCCGGGTTTTTTCATGATCGTTTGTGCATTGACAGGTGCGGCCGTCAGGATTTACTCTTGATTGTTTGGTG
GCGTAAGGGTTTGAGAGCTT

Downstream 100 bases:

>100_bases
GCCCGTGCGATCCCCGCGGCCACCGGTGACCTGGGCTGTACTTCCCTGCTAACCTGAGTCCAGTTTCAAGCCGGAAAAGG
CATGCAGCAGCAGATAGGCA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MANVIVVGAQWGDEGKGKITDLLSVSADVVVRYQGGINAGHTVCVGEQTFKLHLIPSGILYPETCCVIASGTVVDPKVLL
EEIRHLEELGISTKNLFIAETAHVTMPYHRLIDIAEEERRGKNRIGTTGRGIGPTYADKSGRTGFRVLDLMQPEGFREKL
EWIVPLKNVLLERLYNLPPLAVDQVAEQYLAYAEQLRPYVIDASLLIDQAVRTHRNILFEGAQGTLLDLDHGTYPYVTSS
NPVAGGACVGAGVGPTIIDRVIGVAKAYTTRVGEGPFPTELRDEVGRHLGERGAEFGTTTGRPRRCGWFDAVIGRYAVRI
NGLDCLAITKLDVLDELESIKVCVAYHCPSKTVEEFPSDANLFGRCEPVYETLPGWKEPTGHCRTLEELPPKARDYLEFL
AHQMKTPIALVSVGANREQTIIVEDPIHGPKRGLLGHNGD

Sequences:

>Translated_440_residues
MANVIVVGAQWGDEGKGKITDLLSVSADVVVRYQGGINAGHTVCVGEQTFKLHLIPSGILYPETCCVIASGTVVDPKVLL
EEIRHLEELGISTKNLFIAETAHVTMPYHRLIDIAEEERRGKNRIGTTGRGIGPTYADKSGRTGFRVLDLMQPEGFREKL
EWIVPLKNVLLERLYNLPPLAVDQVAEQYLAYAEQLRPYVIDASLLIDQAVRTHRNILFEGAQGTLLDLDHGTYPYVTSS
NPVAGGACVGAGVGPTIIDRVIGVAKAYTTRVGEGPFPTELRDEVGRHLGERGAEFGTTTGRPRRCGWFDAVIGRYAVRI
NGLDCLAITKLDVLDELESIKVCVAYHCPSKTVEEFPSDANLFGRCEPVYETLPGWKEPTGHCRTLEELPPKARDYLEFL
AHQMKTPIALVSVGANREQTIIVEDPIHGPKRGLLGHNGD
>Mature_439_residues
ANVIVVGAQWGDEGKGKITDLLSVSADVVVRYQGGINAGHTVCVGEQTFKLHLIPSGILYPETCCVIASGTVVDPKVLLE
EIRHLEELGISTKNLFIAETAHVTMPYHRLIDIAEEERRGKNRIGTTGRGIGPTYADKSGRTGFRVLDLMQPEGFREKLE
WIVPLKNVLLERLYNLPPLAVDQVAEQYLAYAEQLRPYVIDASLLIDQAVRTHRNILFEGAQGTLLDLDHGTYPYVTSSN
PVAGGACVGAGVGPTIIDRVIGVAKAYTTRVGEGPFPTELRDEVGRHLGERGAEFGTTTGRPRRCGWFDAVIGRYAVRIN
GLDCLAITKLDVLDELESIKVCVAYHCPSKTVEEFPSDANLFGRCEPVYETLPGWKEPTGHCRTLEELPPKARDYLEFLA
HQMKTPIALVSVGANREQTIIVEDPIHGPKRGLLGHNGD

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=426, Percent_Identity=44.131455399061, Blast_Score=346, Evalue=3e-95,
Organism=Homo sapiens, GI34577063, Length=430, Percent_Identity=42.5581395348837, Blast_Score=335, Evalue=3e-92,
Organism=Homo sapiens, GI40316944, Length=397, Percent_Identity=42.5692695214106, Blast_Score=301, Evalue=1e-81,
Organism=Escherichia coli, GI1790620, Length=427, Percent_Identity=48.0093676814988, Blast_Score=398, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI25146553, Length=427, Percent_Identity=44.0281030444965, Blast_Score=333, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI25146555, Length=427, Percent_Identity=44.0281030444965, Blast_Score=332, Evalue=2e-91,
Organism=Saccharomyces cerevisiae, GI6324109, Length=433, Percent_Identity=42.0323325635104, Blast_Score=338, Evalue=1e-93,
Organism=Drosophila melanogaster, GI21356033, Length=425, Percent_Identity=43.5294117647059, Blast_Score=342, Evalue=2e-94,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_GLOVI (Q7NG93)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_926226.1
- ProteinModelPortal:   Q7NG93
- SMR:   Q7NG93
- GeneID:   2601682
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.3280
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- ProtClustDB:   PRK01117
- BioCyc:   GVIO251221:GLR3280-MONOMER
- BRENDA:   6.3.4.4
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 48239; Mature: 48108

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 128-128 BINDING 142-142 BINDING 223-223 BINDING 238-238 BINDING 302-302 BINDING 304-304

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVIVVGAQWGDEGKGKITDLLSVSADVVVRYQGGINAGHTVCVGEQTFKLHLIPSGIL
CCCEEEEECCCCCCCCCHHHHHHHCCEEEEEEECCCCCCCCEEEECCCEEEEEEECCCCC
YPETCCVIASGTVVDPKVLLEEIRHLEELGISTKNLFIAETAHVTMPYHRLIDIAEEERR
CCCCEEEEECCCEECHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCHHHHHHHHHHHHC
GKNRIGTTGRGIGPTYADKSGRTGFRVLDLMQPEGFREKLEWIVPLKNVLLERLYNLPPL
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCH
AVDQVAEQYLAYAEQLRPYVIDASLLIDQAVRTHRNILFEGAQGTLLDLDHGTYPYVTSS
HHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHEEEECCCCCEEECCCCCCCEEECC
NPVAGGACVGAGVGPTIIDRVIGVAKAYTTRVGEGPFPTELRDEVGRHLGERGAEFGTTT
CCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
GRPRRCGWFDAVIGRYAVRINGLDCLAITKLDVLDELESIKVCVAYHCPSKTVEEFPSDA
CCCCCCCHHHHHHHCEEEEECCCEEEEEHHHHHHHHHHHCEEEEEEECCCHHHHHCCCCC
NLFGRCEPVYETLPGWKEPTGHCRTLEELPPKARDYLEFLAHQMKTPIALVSVGANREQT
CCCCCCCHHHHHCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCE
IIVEDPIHGPKRGLLGHNGD
EEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ANVIVVGAQWGDEGKGKITDLLSVSADVVVRYQGGINAGHTVCVGEQTFKLHLIPSGIL
CCEEEEECCCCCCCCCHHHHHHHCCEEEEEEECCCCCCCCEEEECCCEEEEEEECCCCC
YPETCCVIASGTVVDPKVLLEEIRHLEELGISTKNLFIAETAHVTMPYHRLIDIAEEERR
CCCCEEEEECCCEECHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCHHHHHHHHHHHHC
GKNRIGTTGRGIGPTYADKSGRTGFRVLDLMQPEGFREKLEWIVPLKNVLLERLYNLPPL
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCH
AVDQVAEQYLAYAEQLRPYVIDASLLIDQAVRTHRNILFEGAQGTLLDLDHGTYPYVTSS
HHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHEEEECCCCCEEECCCCCCCEEECC
NPVAGGACVGAGVGPTIIDRVIGVAKAYTTRVGEGPFPTELRDEVGRHLGERGAEFGTTT
CCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
GRPRRCGWFDAVIGRYAVRINGLDCLAITKLDVLDELESIKVCVAYHCPSKTVEEFPSDA
CCCCCCCHHHHHHHCEEEEECCCEEEEEHHHHHHHHHHHCEEEEEEECCCHHHHHCCCCC
NLFGRCEPVYETLPGWKEPTGHCRTLEELPPKARDYLEFLAHQMKTPIALVSVGANREQT
CCCCCCCHHHHHCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCE
IIVEDPIHGPKRGLLGHNGD
EEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292