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The map label for this gene is dnaN [H]

Identifier: 37522801

GI number: 37522801

Start: 3439067

End: 3440197

Strand: Direct

Name: dnaN [H]

Synonym: gvip446

Alternate gene names: 37522801

Gene position: 3439067-3440197 (Clockwise)

Preceding gene: 37522799

Following gene: 37522803

Centisome position: 73.82

GC content: 62.07

Gene sequence:

>1131_bases
ATGAAGATTGTTTGTTCACAAGCCGTGTTGAATCAGAACCTATCGCTGGTGGGCCGGGCGGTGCCCTCGCGGCCGACGCA
CCCGATTCTGGCCAATATTTTGCTGGAGGCGGACGGTCGGTCGGGCACGGTAACCCTCACCGGCTTCGACCTCGATCTGG
GTATCGACACGCGTTTTGCAGCCGAGCAGGTCGAAGGGAGCGGACGCACGACGCTGCCGGCGCGGGTGCTCAGCGACATC
ATCTCAAGGCTGCCCAACGAATCGCTGACGATGGAGGTGAGCGAGGACAACGCCATCAGCCTCGTCTGCGGCAGCAGTCA
GTACCAGGTGCAGGGGGCGAGTGCCGAGGAATTTCCGAAGTTGCCCGAACTGCCCGACAGTGCCGCTCACGTTCTGCCGG
TGAGCGAGTTTCTGGAGGGCGTCCAGCGCAGCTTGTTCGCCGCTTCCACTGACGAGAGTAAGCAGATTCTCAACGGTGTC
TCGGTGAAAGCCCTCAAGGAGGGCATGGAGTTCGTGGCCACCGACGCCCATCGCCTGTCGTTCTACCGCACGGACTTTAC
GCTGCCCGAGGCGGGTACGCAGGCCATCGCCGCGGTGCTGCCGGTGCGCTCGGTGCGCGAACTCGAAAAAATCCTGGGGG
CGCAGGCGGGCGACAGCGTCGAGGTGCGCTTCGACGAGAAGCAAATGATCTTCCAGTTTCCCAACCAGACGCTCACCACC
AGGCTTTTGGGTGGCCGCTACCCGGACTACCAGCAGCTACTGCCCAAACAGTTCCAACAGACCGCCGACATGGAGCGCAA
GCGGCTCATCGGCTGCCTGGAGCGGATCGCCGTGTTAGCAGACCAGAAAAACCACATCGTCAAGCTCGACTTTGCCCCGA
GCGAGCACCTGATGACCGTCTCGGTGGACGCTCCCGACGTCGGCCGCGGTCGAGAGAGCGTGCCGGTGCAGTACAGCGGT
CAGGACTTCTCGGTGGCCTTTAACGTGCGCTACTTACTCGAAGGGCTCAAGGCGATGGACGCGACCGATGTGTCGTTTTG
CCTCAACGGAGCGAGCGATCCGGCGGTGCTCAAGCCTGTGGGCGATGGGAATTATCAGTATCTGATCATGCCGGTGAGTA
TTCGGGGGTGA

Upstream 100 bases:

>100_bases
CGTAGATGAGATCGGTCGGTTTGGACTGGCTGGAACGCTAAACGTGGACCTGTCGGGCCGGTGCTATACTCGCTGCCATG
GCCGGGTGTGGTGCGCTGCG

Downstream 100 bases:

>100_bases
GGCGTCAGACGTTGAACCGAAAGTGCATGACGTCGCCGTCTTTGACGATGTACTCCTTGCCCTCGGAGCGCACCTGGCCT
TTTTCGCGGGCGGCCACCTG

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MKIVCSQAVLNQNLSLVGRAVPSRPTHPILANILLEADGRSGTVTLTGFDLDLGIDTRFAAEQVEGSGRTTLPARVLSDI
ISRLPNESLTMEVSEDNAISLVCGSSQYQVQGASAEEFPKLPELPDSAAHVLPVSEFLEGVQRSLFAASTDESKQILNGV
SVKALKEGMEFVATDAHRLSFYRTDFTLPEAGTQAIAAVLPVRSVRELEKILGAQAGDSVEVRFDEKQMIFQFPNQTLTT
RLLGGRYPDYQQLLPKQFQQTADMERKRLIGCLERIAVLADQKNHIVKLDFAPSEHLMTVSVDAPDVGRGRESVPVQYSG
QDFSVAFNVRYLLEGLKAMDATDVSFCLNGASDPAVLKPVGDGNYQYLIMPVSIRG

Sequences:

>Translated_376_residues
MKIVCSQAVLNQNLSLVGRAVPSRPTHPILANILLEADGRSGTVTLTGFDLDLGIDTRFAAEQVEGSGRTTLPARVLSDI
ISRLPNESLTMEVSEDNAISLVCGSSQYQVQGASAEEFPKLPELPDSAAHVLPVSEFLEGVQRSLFAASTDESKQILNGV
SVKALKEGMEFVATDAHRLSFYRTDFTLPEAGTQAIAAVLPVRSVRELEKILGAQAGDSVEVRFDEKQMIFQFPNQTLTT
RLLGGRYPDYQQLLPKQFQQTADMERKRLIGCLERIAVLADQKNHIVKLDFAPSEHLMTVSVDAPDVGRGRESVPVQYSG
QDFSVAFNVRYLLEGLKAMDATDVSFCLNGASDPAVLKPVGDGNYQYLIMPVSIRG
>Mature_376_residues
MKIVCSQAVLNQNLSLVGRAVPSRPTHPILANILLEADGRSGTVTLTGFDLDLGIDTRFAAEQVEGSGRTTLPARVLSDI
ISRLPNESLTMEVSEDNAISLVCGSSQYQVQGASAEEFPKLPELPDSAAHVLPVSEFLEGVQRSLFAASTDESKQILNGV
SVKALKEGMEFVATDAHRLSFYRTDFTLPEAGTQAIAAVLPVRSVRELEKILGAQAGDSVEVRFDEKQMIFQFPNQTLTT
RLLGGRYPDYQQLLPKQFQQTADMERKRLIGCLERIAVLADQKNHIVKLDFAPSEHLMTVSVDAPDVGRGRESVPVQYSG
QDFSVAFNVRYLLEGLKAMDATDVSFCLNGASDPAVLKPVGDGNYQYLIMPVSIRG

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=377, Percent_Identity=27.0557029177719, Blast_Score=135, Evalue=6e-33,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40987; Mature: 40987

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVCSQAVLNQNLSLVGRAVPSRPTHPILANILLEADGRSGTVTLTGFDLDLGIDTRFA
CCEEECHHHHCCCHHHHCCCCCCCCCCHHEEEEEEEECCCCCEEEEEEEEEEECCCCHHH
AEQVEGSGRTTLPARVLSDIISRLPNESLTMEVSEDNAISLVCGSSQYQVQGASAEEFPK
HHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCEEEECCCHHHCCC
LPELPDSAAHVLPVSEFLEGVQRSLFAASTDESKQILNGVSVKALKEGMEFVATDAHRLS
CCCCCCCCHHEEEHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHCCHHHEE
FYRTDFTLPEAGTQAIAAVLPVRSVRELEKILGAQAGDSVEVRFDEKQMIFQFPNQTLTT
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHEEECCCHHHHH
RLLGGRYPDYQQLLPKQFQQTADMERKRLIGCLERIAVLADQKNHIVKLDFAPSEHLMTV
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEE
SVDAPDVGRGRESVPVQYSGQDFSVAFNVRYLLEGLKAMDATDVSFCLNGASDPAVLKPV
EECCCCCCCCCCCCCEEECCCCEEEEEEHHHHHHHHHHCCCCCHHHEECCCCCCCEEEEC
GDGNYQYLIMPVSIRG
CCCCEEEEEEEEEECC
>Mature Secondary Structure
MKIVCSQAVLNQNLSLVGRAVPSRPTHPILANILLEADGRSGTVTLTGFDLDLGIDTRFA
CCEEECHHHHCCCHHHHCCCCCCCCCCHHEEEEEEEECCCCCEEEEEEEEEEECCCCHHH
AEQVEGSGRTTLPARVLSDIISRLPNESLTMEVSEDNAISLVCGSSQYQVQGASAEEFPK
HHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCEEEECCCHHHCCC
LPELPDSAAHVLPVSEFLEGVQRSLFAASTDESKQILNGVSVKALKEGMEFVATDAHRLS
CCCCCCCCHHEEEHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHCCHHHEE
FYRTDFTLPEAGTQAIAAVLPVRSVRELEKILGAQAGDSVEVRFDEKQMIFQFPNQTLTT
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHEEECCCHHHHH
RLLGGRYPDYQQLLPKQFQQTADMERKRLIGCLERIAVLADQKNHIVKLDFAPSEHLMTV
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEE
SVDAPDVGRGRESVPVQYSGQDFSVAFNVRYLLEGLKAMDATDVSFCLNGASDPAVLKPV
EECCCCCCCCCCCCCEEECCCCEEEEEEHHHHHHHHHHCCCCCHHHEECCCCCCCEEEEC
GDGNYQYLIMPVSIRG
CCCCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8654968 [H]