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The map label for this gene is stp [H]

Identifier: 37522391

GI number: 37522391

Start: 3002869

End: 3004458

Strand: Reverse

Name: stp [H]

Synonym: gll2822

Alternate gene names: 37522391

Gene position: 3004458-3002869 (Counterclockwise)

Preceding gene: 37522392

Following gene: 37522388

Centisome position: 64.49

GC content: 62.14

Gene sequence:

>1590_bases
ATGACCAAGCAAGTTGCCCGGAATACCACACGCGACCTGGGTGCCCAGCCCGACAACAAGTGGGCGATCTTACTGGCGGT
CAGCATCGGCACTTTTTTGGTGCCGCTCGACATCACTGTGGTCGCCCTGGCCCTACCCGACATCGAAAGCTCGCTGCGCA
CGAGCTTTGCCGATCTCCAGTGGATCATCAATGGTTACAACCTGTCATTTACCGCCTTTTTGTTGGCGGCCGGAACGCTG
GCGGACCGCTTCGGCCGCCGTCGGCTGTTTCTGGTGGGCCTTGCGCTGTTTACCCTGACTTCGCTCACTTGCGGGCTCGC
CACCACCCCCCTGACGCTCACCCTCGCCCGCAGCATCCAGGGGTTGGGCGGTGCGCTGCTGCTCATCTCCTCGCTGGCGA
TTCTGTCCCAAACCTTCCGCGAAGGACCGGAGCGAGCCAACGCCTTCGGTGTCTGGGGATTGGTGATGGGGGTGGGCGCA
TCGCTCGGGCCGATGATTGGCGGCCTGCTGGTGGATCTGCTCAGCTGGCGGTGGATCTTTTTGCTCAACGTGCCGATCGG
TGTCGGCCTAGTCGCCTTTACCTTGAGCAAAGTGGACGAGTCGCGCGATCCCGGAGCGGCCCGGGTCGATTGGCTGGGAT
TGACCACATTCACTTCGGCTTTGTTTTCGCTGTGTTTCGCCTTGATCCAGGGCAACGAGCTGGGTTGGACCAGCGCTGCC
ATTCTCGGCTTGTTGGCAGCGGCGGCCGTGCTGCTGTTCGCCTTTGCGCTCATTGAGCGGCGGCAGAGCCAGCCGATGTT
CGACCTGTCGCTTTTGGGCAACCCCGCCTTTGTGGGTACTTCGTTGCTCGCGGCGAGCAACGGCGCTTCGTTTTGGGCAA
TGATCGTCTATCTGCCCCTGTTTTTTCAAAACGTTCTCGGTTACTCGCCTTTGCAAGCCGGGCTGATGCTGTTGCCGCTC
ACGGTGCCTTTGCTGGTCTGCCCGCCTCTGGGCGGTCGGCTGGCCCAGGTACTGCCCGCCCGGCTGCTGCTCAGCGGCGG
CATGCTCATGGTCGGCCTGGGCTTTGTTTGGATGTACGGGGTCAACGCGGGTTCCCCCTGGACCGCGTTTTTGCCGGGCT
TTTTGTTGGCCGGGATCGGAGCCGGGCTGATTAACGCAGAGATCGCGAACGTGGCTATTGCCGTGTTGCCCCCTCAGCGC
AGCGGCATGGGTTCGGGAATCACCAGCACCTTTCGCCACGGCGCCCACGCTCTGGGTATCGCGCTACTTGGCTCGCTACT
CACCCAGCGGGTTGCCACCAGCCTGGAGCAATCGACCCTACCCGGCCTTGTTACCGAGCGCAGCACGCAACTGGCAAGCC
AGATTGCGGCCGGCGACCTGATCGGAGTGGTCCGCACGCTGCCGCCGGAGCTGCAAAACGCGTTTGCCGCAGCGGCCCAA
AACAGTTTCATCGACGGTTTTAACCTGATCGTGCTCCTGGCGGCTGCCACGGCGCTGCTGGGTGCCCTGGCCACCTTTGT
TCTGGTAAAAGATCAGCGTTCCCAAAGCCAACTCCAAAGACAAGAACGTCCAAGTGCCTCGAAGAGCTAA

Upstream 100 bases:

>100_bases
CGCCGACCCACAAACGGCCTGGCAGTTCCTGTACTGGCTGGATGTATTGCCCTTCTAGCCCGATGCGTCTACCCATCAAC
CATGAGGAGGTCAAACGCCA

Downstream 100 bases:

>100_bases
AAGTCAGCGGCTCCTGCTTGTGGGGTCAGACTGTGCTGCAACCGGTGAGCAGTGCTTTTTGGCCCTTCAGGTTTCCGGAC
GGACCTGCCCGGAGATATCG

Product: hypothetical protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Spectinomycin tetracycline efflux pump [H]

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MTKQVARNTTRDLGAQPDNKWAILLAVSIGTFLVPLDITVVALALPDIESSLRTSFADLQWIINGYNLSFTAFLLAAGTL
ADRFGRRRLFLVGLALFTLTSLTCGLATTPLTLTLARSIQGLGGALLLISSLAILSQTFREGPERANAFGVWGLVMGVGA
SLGPMIGGLLVDLLSWRWIFLLNVPIGVGLVAFTLSKVDESRDPGAARVDWLGLTTFTSALFSLCFALIQGNELGWTSAA
ILGLLAAAAVLLFAFALIERRQSQPMFDLSLLGNPAFVGTSLLAASNGASFWAMIVYLPLFFQNVLGYSPLQAGLMLLPL
TVPLLVCPPLGGRLAQVLPARLLLSGGMLMVGLGFVWMYGVNAGSPWTAFLPGFLLAGIGAGLINAEIANVAIAVLPPQR
SGMGSGITSTFRHGAHALGIALLGSLLTQRVATSLEQSTLPGLVTERSTQLASQIAAGDLIGVVRTLPPELQNAFAAAAQ
NSFIDGFNLIVLLAAATALLGALATFVLVKDQRSQSQLQRQERPSASKS

Sequences:

>Translated_529_residues
MTKQVARNTTRDLGAQPDNKWAILLAVSIGTFLVPLDITVVALALPDIESSLRTSFADLQWIINGYNLSFTAFLLAAGTL
ADRFGRRRLFLVGLALFTLTSLTCGLATTPLTLTLARSIQGLGGALLLISSLAILSQTFREGPERANAFGVWGLVMGVGA
SLGPMIGGLLVDLLSWRWIFLLNVPIGVGLVAFTLSKVDESRDPGAARVDWLGLTTFTSALFSLCFALIQGNELGWTSAA
ILGLLAAAAVLLFAFALIERRQSQPMFDLSLLGNPAFVGTSLLAASNGASFWAMIVYLPLFFQNVLGYSPLQAGLMLLPL
TVPLLVCPPLGGRLAQVLPARLLLSGGMLMVGLGFVWMYGVNAGSPWTAFLPGFLLAGIGAGLINAEIANVAIAVLPPQR
SGMGSGITSTFRHGAHALGIALLGSLLTQRVATSLEQSTLPGLVTERSTQLASQIAAGDLIGVVRTLPPELQNAFAAAAQ
NSFIDGFNLIVLLAAATALLGALATFVLVKDQRSQSQLQRQERPSASKS
>Mature_528_residues
TKQVARNTTRDLGAQPDNKWAILLAVSIGTFLVPLDITVVALALPDIESSLRTSFADLQWIINGYNLSFTAFLLAAGTLA
DRFGRRRLFLVGLALFTLTSLTCGLATTPLTLTLARSIQGLGGALLLISSLAILSQTFREGPERANAFGVWGLVMGVGAS
LGPMIGGLLVDLLSWRWIFLLNVPIGVGLVAFTLSKVDESRDPGAARVDWLGLTTFTSALFSLCFALIQGNELGWTSAAI
LGLLAAAAVLLFAFALIERRQSQPMFDLSLLGNPAFVGTSLLAASNGASFWAMIVYLPLFFQNVLGYSPLQAGLMLLPLT
VPLLVCPPLGGRLAQVLPARLLLSGGMLMVGLGFVWMYGVNAGSPWTAFLPGFLLAGIGAGLINAEIANVAIAVLPPQRS
GMGSGITSTFRHGAHALGIALLGSLLTQRVATSLEQSTLPGLVTERSTQLASQIAAGDLIGVVRTLPPELQNAFAAAAQN
SFIDGFNLIVLLAAATALLGALATFVLVKDQRSQSQLQRQERPSASKS

Specific function: Contributes to spectinomycin and tetracycline resistance [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=423, Percent_Identity=29.0780141843972, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI87081983, Length=420, Percent_Identity=30.2380952380952, Blast_Score=152, Evalue=7e-38,
Organism=Escherichia coli, GI1788710, Length=479, Percent_Identity=26.9311064718163, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1790195, Length=420, Percent_Identity=29.7619047619048, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1788392, Length=307, Percent_Identity=35.1791530944625, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1790146, Length=178, Percent_Identity=29.2134831460674, Blast_Score=70, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324954, Length=285, Percent_Identity=28.4210526315789, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6319770, Length=490, Percent_Identity=25.9183673469388, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6322958, Length=413, Percent_Identity=24.2130750605327, Blast_Score=80, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6325455, Length=337, Percent_Identity=22.2551928783383, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323735, Length=354, Percent_Identity=25.9887005649718, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6321663, Length=152, Percent_Identity=28.2894736842105, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55610; Mature: 55479

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKQVARNTTRDLGAQPDNKWAILLAVSIGTFLVPLDITVVALALPDIESSLRTSFADLQ
CCCHHHHCCHHHCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
WIINGYNLSFTAFLLAAGTLADRFGRRRLFLVGLALFTLTSLTCGLATTPLTLTLARSIQ
HHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
GLGGALLLISSLAILSQTFREGPERANAFGVWGLVMGVGASLGPMIGGLLVDLLSWRWIF
CCCHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LLNVPIGVGLVAFTLSKVDESRDPGAARVDWLGLTTFTSALFSLCFALIQGNELGWTSAA
EEECCHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
ILGLLAAAAVLLFAFALIERRQSQPMFDLSLLGNPAFVGTSLLAASNGASFWAMIVYLPL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHH
FFQNVLGYSPLQAGLMLLPLTVPLLVCPPLGGRLAQVLPARLLLSGGMLMVGLGFVWMYG
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHC
VNAGSPWTAFLPGFLLAGIGAGLINAEIANVAIAVLPPQRSGMGSGITSTFRHGAHALGI
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHHEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
ALLGSLLTQRVATSLEQSTLPGLVTERSTQLASQIAAGDLIGVVRTLPPELQNAFAAAAQ
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
NSFIDGFNLIVLLAAATALLGALATFVLVKDQRSQSQLQRQERPSASKS
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TKQVARNTTRDLGAQPDNKWAILLAVSIGTFLVPLDITVVALALPDIESSLRTSFADLQ
CCHHHHCCHHHCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
WIINGYNLSFTAFLLAAGTLADRFGRRRLFLVGLALFTLTSLTCGLATTPLTLTLARSIQ
HHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
GLGGALLLISSLAILSQTFREGPERANAFGVWGLVMGVGASLGPMIGGLLVDLLSWRWIF
CCCHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LLNVPIGVGLVAFTLSKVDESRDPGAARVDWLGLTTFTSALFSLCFALIQGNELGWTSAA
EEECCHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
ILGLLAAAAVLLFAFALIERRQSQPMFDLSLLGNPAFVGTSLLAASNGASFWAMIVYLPL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHH
FFQNVLGYSPLQAGLMLLPLTVPLLVCPPLGGRLAQVLPARLLLSGGMLMVGLGFVWMYG
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHC
VNAGSPWTAFLPGFLLAGIGAGLINAEIANVAIAVLPPQRSGMGSGITSTFRHGAHALGI
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHHEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
ALLGSLLTQRVATSLEQSTLPGLVTERSTQLASQIAAGDLIGVVRTLPPELQNAFAAAAQ
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
NSFIDGFNLIVLLAAATALLGALATFVLVKDQRSQSQLQRQERPSASKS
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]