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The map label for this gene is ntrB [H]

Identifier: 37521231

GI number: 37521231

Start: 1776263

End: 1777669

Strand: Reverse

Name: ntrB [H]

Synonym: gll1662

Alternate gene names: 37521231

Gene position: 1777669-1776263 (Counterclockwise)

Preceding gene: 37521232

Following gene: 37521230

Centisome position: 38.16

GC content: 63.68

Gene sequence:

>1407_bases
ATGCTCCACGATCCCCAACAACCGGAACTGTTTCCGAAGCTCTCCGCCGAAGAACTCAAGCGGCTGGGTGAACACGGTCG
CGAGGTGGATCTGGCGCCGGGGCAGGTGATCTTTCAAGAAGGGGACCCGGTCTCCCGCTTCTTCGTCGTCCTCCAAGGAC
AAGTGCAGGTCACCAAACGGGTGGACGGTCAGGATCAACTGCTGACCGTTCACCAGCCGGGCGAATTTACGGGCGAAATT
TCGATGGTCACCGGCGGCCCCTCGCGGGCCACCGGCCGCTCCCTGGGGGTTAGCCGGGTGCTTGAAATCGAGCCGGAGGC
GTTCAAGCGCGTCCTTGCCGAATGTTCCCAGGGGGCGGCGGTGATCTTGGCGGCGATGGCGGGACGGGCCACGGACCTCG
AAGGGCAGATGCGTCAGCAAGAAAAACTGGCCGCCCTCGGCAAACTCGCCGCCGGACTCGCCCACGAACTGAACAACCCC
GCCGCCGCCGGTCGCCGGGCTGCCCAACAGTTGCGCGAAGCGCTCGCGAGTGTCCAGACCCGCCTGCTCGGGCTGTGCGA
AGGGCTGTTCGCTCCCCAGGAGCGCAACTTACTCGTGGCATTGCAAAAAGCAGCGATCGGCCATGCCGCCTGTGCGCCGC
GCCTCGACCCGCTCGATCAAAGCGACCGCGAGGAATCCCTGGGCGAATGGCTGGAGGAGCGGGGCATCGCAAACGCCTGG
GAGGCTTCTCCGGCGCTGGTGGCGGCGGGCATCGACCCCGACAAGCTCACTCCCCTGGCCGAAGGCTGCACCCCGGAGGC
GTTCGGCGAAGCGCTCCACTGGCTGGTCGAGACGCTCACCCTGGCCGAGCTGGTCAACCAGGTGGACCAGAGCACCGGCC
GCATCTCGCAACTGGTCAAGGCGATCAAGTCCTACTCCTATATGGATCAGGCGCCTTTGCAGGAAATCGACGTGCACGAA
GGACTCGACAACACGCTCACCATCCTTCACCACAAGCTCAAGTACGGCATCACCGTCCACCGCCGGTACGCCCCAAATCT
GCCGCGCATCTTTGCCCACGGCAGCGAACTCAATCAAGTCTGGACGAATCTGATCGACAACGCTGCCTTCGCCATGCAGG
GCAAGGGTGAACTGACCATCCGCACCGCCCCCGGTCCGGATACGGTCCATATCGAAATTACCGACAACGGCCCCGGTATC
CCGCTGGAGATCCAGACGCGCATCTTCGAGCCGTTTTTTACCACCAAAGGGGTCGGCGAAGGTTCAGGTTTGGGTCTCGA
TATTGCTCGCCGCATCGTCGTCAAGCACCACCACGGCACGATCCGCGTCACCTCCAAACCCGGCGAGACGCGCTTTAGCA
TCTGTCTGCCCGTCCGGCAGCCCAAGCAACAACCAGGGGAGAAATAG

Upstream 100 bases:

>100_bases
TACCGCCCCGAGTTGTGATCCCCGGCCCGCCACCCCCCCAAAGGCGGGCTGCCTCCATAAAATAAGGGACGGGCGCCCCG
ACAGCCAGGCAGGATTCGCC

Downstream 100 bases:

>100_bases
CGATGCTCGACATCGAGACTTTGCAGCGGGTGCCCCTATTTGCCAATCTTCCGGAGGAGCGGCTGCACTGGCTTCTGGGC
GAGGGCCGGGAGCTGTGGCT

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MLHDPQQPELFPKLSAEELKRLGEHGREVDLAPGQVIFQEGDPVSRFFVVLQGQVQVTKRVDGQDQLLTVHQPGEFTGEI
SMVTGGPSRATGRSLGVSRVLEIEPEAFKRVLAECSQGAAVILAAMAGRATDLEGQMRQQEKLAALGKLAAGLAHELNNP
AAAGRRAAQQLREALASVQTRLLGLCEGLFAPQERNLLVALQKAAIGHAACAPRLDPLDQSDREESLGEWLEERGIANAW
EASPALVAAGIDPDKLTPLAEGCTPEAFGEALHWLVETLTLAELVNQVDQSTGRISQLVKAIKSYSYMDQAPLQEIDVHE
GLDNTLTILHHKLKYGITVHRRYAPNLPRIFAHGSELNQVWTNLIDNAAFAMQGKGELTIRTAPGPDTVHIEITDNGPGI
PLEIQTRIFEPFFTTKGVGEGSGLGLDIARRIVVKHHHGTIRVTSKPGETRFSICLPVRQPKQQPGEK

Sequences:

>Translated_468_residues
MLHDPQQPELFPKLSAEELKRLGEHGREVDLAPGQVIFQEGDPVSRFFVVLQGQVQVTKRVDGQDQLLTVHQPGEFTGEI
SMVTGGPSRATGRSLGVSRVLEIEPEAFKRVLAECSQGAAVILAAMAGRATDLEGQMRQQEKLAALGKLAAGLAHELNNP
AAAGRRAAQQLREALASVQTRLLGLCEGLFAPQERNLLVALQKAAIGHAACAPRLDPLDQSDREESLGEWLEERGIANAW
EASPALVAAGIDPDKLTPLAEGCTPEAFGEALHWLVETLTLAELVNQVDQSTGRISQLVKAIKSYSYMDQAPLQEIDVHE
GLDNTLTILHHKLKYGITVHRRYAPNLPRIFAHGSELNQVWTNLIDNAAFAMQGKGELTIRTAPGPDTVHIEITDNGPGI
PLEIQTRIFEPFFTTKGVGEGSGLGLDIARRIVVKHHHGTIRVTSKPGETRFSICLPVRQPKQQPGEK
>Mature_468_residues
MLHDPQQPELFPKLSAEELKRLGEHGREVDLAPGQVIFQEGDPVSRFFVVLQGQVQVTKRVDGQDQLLTVHQPGEFTGEI
SMVTGGPSRATGRSLGVSRVLEIEPEAFKRVLAECSQGAAVILAAMAGRATDLEGQMRQQEKLAALGKLAAGLAHELNNP
AAAGRRAAQQLREALASVQTRLLGLCEGLFAPQERNLLVALQKAAIGHAACAPRLDPLDQSDREESLGEWLEERGIANAW
EASPALVAAGIDPDKLTPLAEGCTPEAFGEALHWLVETLTLAELVNQVDQSTGRISQLVKAIKSYSYMDQAPLQEIDVHE
GLDNTLTILHHKLKYGITVHRRYAPNLPRIFAHGSELNQVWTNLIDNAAFAMQGKGELTIRTAPGPDTVHIEITDNGPGI
PLEIQTRIFEPFFTTKGVGEGSGLGLDIARRIVVKHHHGTIRVTSKPGETRFSICLPVRQPKQQPGEK

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=154, Percent_Identity=34.4155844155844, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1788549, Length=182, Percent_Identity=31.3186813186813, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1790436, Length=185, Percent_Identity=30.8108108108108, Blast_Score=79, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50862; Mature: 50862

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS50042 CNMP_BINDING_3 ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHDPQQPELFPKLSAEELKRLGEHGREVDLAPGQVIFQEGDPVSRFFVVLQGQVQVTKR
CCCCCCCCCCCCCCCHHHHHHHHHCCCEEECCCCEEEEECCCCCEEEEEEEECCEEEEEC
VDGQDQLLTVHQPGEFTGEISMVTGGPSRATGRSLGVSRVLEIEPEAFKRVLAECSQGAA
CCCCCCEEEEECCCCCCCEEEEEECCCCCCCCCCCCCHHEEEECHHHHHHHHHHHCCCHH
VILAAMAGRATDLEGQMRQQEKLAALGKLAAGLAHELNNPAAAGRRAAQQLREALASVQT
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
RLLGLCEGLFAPQERNLLVALQKAAIGHAACAPRLDPLDQSDREESLGEWLEERGIANAW
HHHHHHHHHCCCCCCCEEEEEHHHHHCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
EASPALVAAGIDPDKLTPLAEGCTPEAFGEALHWLVETLTLAELVNQVDQSTGRISQLVK
CCCCCEEEECCCHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIKSYSYMDQAPLQEIDVHEGLDNTLTILHHKLKYGITVHRRYAPNLPRIFAHGSELNQV
HHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHCEEEEECCCCCCCHHHHCCHHHHHH
WTNLIDNAAFAMQGKGELTIRTAPGPDTVHIEITDNGPGIPLEIQTRIFEPFFTTKGVGE
HHHHHCCCCEEECCCCCEEEEECCCCCEEEEEEECCCCCCCEEEHHHHHCHHHHCCCCCC
GSGLGLDIARRIVVKHHHGTIRVTSKPGETRFSICLPVRQPKQQPGEK
CCCCCHHHHHHHHEEECCCEEEEECCCCCCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MLHDPQQPELFPKLSAEELKRLGEHGREVDLAPGQVIFQEGDPVSRFFVVLQGQVQVTKR
CCCCCCCCCCCCCCCHHHHHHHHHCCCEEECCCCEEEEECCCCCEEEEEEEECCEEEEEC
VDGQDQLLTVHQPGEFTGEISMVTGGPSRATGRSLGVSRVLEIEPEAFKRVLAECSQGAA
CCCCCCEEEEECCCCCCCEEEEEECCCCCCCCCCCCCHHEEEECHHHHHHHHHHHCCCHH
VILAAMAGRATDLEGQMRQQEKLAALGKLAAGLAHELNNPAAAGRRAAQQLREALASVQT
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
RLLGLCEGLFAPQERNLLVALQKAAIGHAACAPRLDPLDQSDREESLGEWLEERGIANAW
HHHHHHHHHCCCCCCCEEEEEHHHHHCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
EASPALVAAGIDPDKLTPLAEGCTPEAFGEALHWLVETLTLAELVNQVDQSTGRISQLVK
CCCCCEEEECCCHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIKSYSYMDQAPLQEIDVHEGLDNTLTILHHKLKYGITVHRRYAPNLPRIFAHGSELNQV
HHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHCEEEEECCCCCCCHHHHCCHHHHHH
WTNLIDNAAFAMQGKGELTIRTAPGPDTVHIEITDNGPGIPLEIQTRIFEPFFTTKGVGE
HHHHHCCCCEEECCCCCEEEEECCCCCEEEEEEECCCCCCCEEEHHHHHCHHHHCCCCCC
GSGLGLDIARRIVVKHHHGTIRVTSKPGETRFSICLPVRQPKQQPGEK
CCCCCHHHHHHHHEEECCCEEEEECCCCCCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2710108; 8355615 [H]