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The map label for this gene is ntrB [H]

Identifier: 37521230

GI number: 37521230

Start: 1774860

End: 1776260

Strand: Reverse

Name: ntrB [H]

Synonym: gll1661

Alternate gene names: 37521230

Gene position: 1776260-1774860 (Counterclockwise)

Preceding gene: 37521231

Following gene: 37521228

Centisome position: 38.13

GC content: 63.45

Gene sequence:

>1401_bases
ATGCTCGACATCGAGACTTTGCAGCGGGTGCCCCTATTTGCCAATCTTCCGGAGGAGCGGCTGCACTGGCTTCTGGGCGA
GGGCCGGGAGCTGTGGCTGGAGCCGGGACAGATCCATCGCGCCGCCGGGGAGCCCGCGGATTACGTCTTTGTGATCCTCG
AAGGCGAGATCCAGATCGTCAAACACGAGGGTCAGCAGGAGATTTTGCTGGTCACCCACGTCCCTTACACCCTCTACGGC
GAATTGCCCATTTTGACGGGAGATCCCCACTACTGGGCGAGCGGCCGGGCCCAGGTGCGCTGCCGGATCTTCGAATTCGG
CAAGGAGGCCTTCTGGGAAATGCTCTCCTCCTGCAGCTGTGTAACGACGAGCATCCTACGCACGATGGCCCTGCGGATGC
AGGCGGTGCAGACGCTCTCGCAGCACCGCGAAAAACTGGTCGCCCTCGGCAATCTTGCCGCCGGACTCGCCCACGAACTG
AATAACCCCGCCGCCGCCGCCCAGCGCGCCGCCCGGCGGCTGCAGGAAATCTTTCAGGTGCTCCAGCCTGCCGCTTTGCA
GTTGGGCCGCCAGGTGGTCGACTGCGAGCAGCAGGCGTTTTTGGTGGGCCTGCAGCGCGAAGCGCTCGCCAGGGCCGCGA
GTACCTCCAGACTCGATCCGCTCACCCGCAGCGACCGCGAAGAGGAGATTGCCGCCTGGCTCGAAACGCACGGTGTGACG
CAGTGCTGGCAACTGGCGCCGACGCTGGTGGGGGCGGGCCTCGACACCGACTGGCTAGCCAAGATCGCCGAGCGGATGCC
CGCCCCGGCCCTGGACAAGGTGCTTGTCTGGCTGGAGGCCAATCTCAGTGGGGTCGGGCTGTTGACGGAATTGGTCCAGA
GCACCGAACGGATCTCAAAGCTCGTCAAGTCGATCAAAGACTACTCCTACCTCGATCAAGCCCCGCTGCAGGAGGTCGAT
ATCCACCAGGGGCTGGAGAGCACCCTGACCATGCTCGGCCACCGGCTCAATGGGTCGATCGCTGTCGAGCGCGATTACGA
CTACACCTTGCCTGCGATTACCGCCTACGGCAGCGAACTCAACCAGGTCTGGACGAACCTCATCGACAACGCCATCGACG
CGCTCGAAGCGAATGGGTGCGGATGCATCCGCATCCGCACCCGGCATGAGCACGAGGTGGTGCTGGTCGAAATTGCCGAC
GACGGCCCCGGTATTCCGGCTGCAGCCCAATCCCATATCTTCGACCCGTTTTTTACCACCAAGGCTGTGGGCGAAGGCAC
CGGCCTCGGGTTGCATATCGTCTATCAGGTCGTGGTCGTGCAGCACCATGGCGATGTCCGCTTACTCTGCGAACCGGGAG
ACACCCGCTTTCAGGTGCGCTTGCCCCTCCGTATCGGTTGA

Upstream 100 bases:

>100_bases
ACCACGGCACGATCCGCGTCACCTCCAAACCCGGCGAGACGCGCTTTAGCATCTGTCTGCCCGTCCGGCAGCCCAAGCAA
CAACCAGGGGAGAAATAGCG

Downstream 100 bases:

>100_bases
TCAGCCAGGCTATCGGACTCAGACCCGGCTTACGAAGTGCAGCACGATGGCAAAGTGATCTTCAAAAAAGAGATCTTCAT
GGATGTACAGATCCGCAAGC

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MLDIETLQRVPLFANLPEERLHWLLGEGRELWLEPGQIHRAAGEPADYVFVILEGEIQIVKHEGQQEILLVTHVPYTLYG
ELPILTGDPHYWASGRAQVRCRIFEFGKEAFWEMLSSCSCVTTSILRTMALRMQAVQTLSQHREKLVALGNLAAGLAHEL
NNPAAAAQRAARRLQEIFQVLQPAALQLGRQVVDCEQQAFLVGLQREALARAASTSRLDPLTRSDREEEIAAWLETHGVT
QCWQLAPTLVGAGLDTDWLAKIAERMPAPALDKVLVWLEANLSGVGLLTELVQSTERISKLVKSIKDYSYLDQAPLQEVD
IHQGLESTLTMLGHRLNGSIAVERDYDYTLPAITAYGSELNQVWTNLIDNAIDALEANGCGCIRIRTRHEHEVVLVEIAD
DGPGIPAAAQSHIFDPFFTTKAVGEGTGLGLHIVYQVVVVQHHGDVRLLCEPGDTRFQVRLPLRIG

Sequences:

>Translated_466_residues
MLDIETLQRVPLFANLPEERLHWLLGEGRELWLEPGQIHRAAGEPADYVFVILEGEIQIVKHEGQQEILLVTHVPYTLYG
ELPILTGDPHYWASGRAQVRCRIFEFGKEAFWEMLSSCSCVTTSILRTMALRMQAVQTLSQHREKLVALGNLAAGLAHEL
NNPAAAAQRAARRLQEIFQVLQPAALQLGRQVVDCEQQAFLVGLQREALARAASTSRLDPLTRSDREEEIAAWLETHGVT
QCWQLAPTLVGAGLDTDWLAKIAERMPAPALDKVLVWLEANLSGVGLLTELVQSTERISKLVKSIKDYSYLDQAPLQEVD
IHQGLESTLTMLGHRLNGSIAVERDYDYTLPAITAYGSELNQVWTNLIDNAIDALEANGCGCIRIRTRHEHEVVLVEIAD
DGPGIPAAAQSHIFDPFFTTKAVGEGTGLGLHIVYQVVVVQHHGDVRLLCEPGDTRFQVRLPLRIG
>Mature_466_residues
MLDIETLQRVPLFANLPEERLHWLLGEGRELWLEPGQIHRAAGEPADYVFVILEGEIQIVKHEGQQEILLVTHVPYTLYG
ELPILTGDPHYWASGRAQVRCRIFEFGKEAFWEMLSSCSCVTTSILRTMALRMQAVQTLSQHREKLVALGNLAAGLAHEL
NNPAAAAQRAARRLQEIFQVLQPAALQLGRQVVDCEQQAFLVGLQREALARAASTSRLDPLTRSDREEEIAAWLETHGVT
QCWQLAPTLVGAGLDTDWLAKIAERMPAPALDKVLVWLEANLSGVGLLTELVQSTERISKLVKSIKDYSYLDQAPLQEVD
IHQGLESTLTMLGHRLNGSIAVERDYDYTLPAITAYGSELNQVWTNLIDNAIDALEANGCGCIRIRTRHEHEVVLVEIAD
DGPGIPAAAQSHIFDPFFTTKAVGEGTGLGLHIVYQVVVVQHHGDVRLLCEPGDTRFQVRLPLRIG

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=154, Percent_Identity=33.7662337662338, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788549, Length=179, Percent_Identity=31.8435754189944, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1790436, Length=177, Percent_Identity=31.638418079096, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI145693157, Length=191, Percent_Identity=28.7958115183246, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51731; Mature: 51731

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS50042 CNMP_BINDING_3 ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDIETLQRVPLFANLPEERLHWLLGEGRELWLEPGQIHRAAGEPADYVFVILEGEIQIV
CCCHHHHHCCCCCCCCCHHHHHHHHCCCCEEECCCCHHHHCCCCCCCEEEEEEECCEEEE
KHEGQQEILLVTHVPYTLYGELPILTGDPHYWASGRAQVRCRIFEFGKEAFWEMLSSCSC
EECCCCEEEEEEECCEEEECCEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCHH
VTTSILRTMALRMQAVQTLSQHREKLVALGNLAAGLAHELNNPAAAAQRAARRLQEIFQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LQPAALQLGRQVVDCEQQAFLVGLQREALARAASTSRLDPLTRSDREEEIAAWLETHGVT
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHH
QCWQLAPTLVGAGLDTDWLAKIAERMPAPALDKVLVWLEANLSGVGLLTELVQSTERISK
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHEEEEEECCCCCHHHHHHHHHHHHHHHH
LVKSIKDYSYLDQAPLQEVDIHQGLESTLTMLGHRLNGSIAVERDYDYTLPAITAYGSEL
HHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHH
NQVWTNLIDNAIDALEANGCGCIRIRTRHEHEVVLVEIADDGPGIPAAAQSHIFDPFFTT
HHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEECCCCCCCCCHHHHHCCCCHHHH
KAVGEGTGLGLHIVYQVVVVQHHGDVRLLCEPGDTRFQVRLPLRIG
HHCCCCCCCHHHHHHHHHHEEECCCEEEEECCCCCEEEEEECEECC
>Mature Secondary Structure
MLDIETLQRVPLFANLPEERLHWLLGEGRELWLEPGQIHRAAGEPADYVFVILEGEIQIV
CCCHHHHHCCCCCCCCCHHHHHHHHCCCCEEECCCCHHHHCCCCCCCEEEEEEECCEEEE
KHEGQQEILLVTHVPYTLYGELPILTGDPHYWASGRAQVRCRIFEFGKEAFWEMLSSCSC
EECCCCEEEEEEECCEEEECCEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCHH
VTTSILRTMALRMQAVQTLSQHREKLVALGNLAAGLAHELNNPAAAAQRAARRLQEIFQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LQPAALQLGRQVVDCEQQAFLVGLQREALARAASTSRLDPLTRSDREEEIAAWLETHGVT
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHH
QCWQLAPTLVGAGLDTDWLAKIAERMPAPALDKVLVWLEANLSGVGLLTELVQSTERISK
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHEEEEEECCCCCHHHHHHHHHHHHHHHH
LVKSIKDYSYLDQAPLQEVDIHQGLESTLTMLGHRLNGSIAVERDYDYTLPAITAYGSEL
HHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHH
NQVWTNLIDNAIDALEANGCGCIRIRTRHEHEVVLVEIADDGPGIPAAAQSHIFDPFFTT
HHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEECCCCCCCCCHHHHHCCCCHHHH
KAVGEGTGLGLHIVYQVVVVQHHGDVRLLCEPGDTRFQVRLPLRIG
HHCCCCCCCHHHHHHHHHHEEECCCEEEEECCCCCEEEEEECEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2710108; 8355615 [H]