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The map label for this gene is trpF

Identifier: 37520323

GI number: 37520323

Start: 804080

End: 804748

Strand: Reverse

Name: trpF

Synonym: gvip085

Alternate gene names: 37520323

Gene position: 804748-804080 (Counterclockwise)

Preceding gene: 37520325

Following gene: 37520322

Centisome position: 17.27

GC content: 66.52

Gene sequence:

>669_bases
ATGCGCGTAAAAATCTGCGGCTTCACCGACCCGGGCCAAGCCCAGGCCGCCGCCCGGTTGGGAGTGCACGCCCTGGGCTT
TGTCTGTGTCCCCGGCACCCCCCGCTATGTGGACGCGGCCCGCCTGCGCGAGATCGCCGCCGCCCTGCCGCCGTTCACCT
TCAAAGTCGGCGTCTTCGTGGATGCGCCCGTTGAGGCGATGCGCGCCGCGGTGGAAGCGGGCGAGCTGCAGGGGGTCCAG
CTCCACGGCGAGGAGAGCCCCGAGACCTGCGCTCACCTGGCGCGGACGCTTCCTGGGATTTTGCGGATCAAGGCTCTGCG
CGTCCGGGAACCGGCGGACCTCGAAAAGATCGCACTGTACGTGGATGCCATCGAAGCGGTCCTACTCGACGCCTGGCATC
CCACCCAGGCGGGCGGGACAGGACGAACCCTCGACTGGAAGGCGCTGCAGGGGTTTTGCCCGGCGCTGCCGTGGATGCTT
TCGGGCGGTCTGCGGGCCGACAATCTGGCCCAAGCCCTGGACATCCTGACACCCGATGCGGTTGACCTGTCGAGCGGCGT
CGAGAATGGGGTACCTGGACAAAAAGATCTGAGCAAAATCACTCAGATCTTGCACATCGCCCAAGGCAACACCGGGCAAC
GCCGACCGCCCCTTTGCGATGGAACGTGA

Upstream 100 bases:

>100_bases
GCAGCAGGGCGGGTCCGGGCTTCATCGGTTGGAGCCGTCCAAAAGTACGCAGCAACCCTAACCCACGGCTAGCATCGTAG
GCAAGAAACACCCGTCTACC

Downstream 100 bases:

>100_bases
CTGCGGCGAATAGACGCGGGCGGTTTATGATTAATGATGAAGGCACTGTTACCTTTCTTAATTTCAGTGCGCAGCTGGAG
AAATGAGCGTGGTGTCCGCA

Product: N-(5'-phosphoribosyl)anthranilate isomerase

Products: NA

Alternate protein names: PRAI

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MRVKICGFTDPGQAQAAARLGVHALGFVCVPGTPRYVDAARLREIAAALPPFTFKVGVFVDAPVEAMRAAVEAGELQGVQ
LHGEESPETCAHLARTLPGILRIKALRVREPADLEKIALYVDAIEAVLLDAWHPTQAGGTGRTLDWKALQGFCPALPWML
SGGLRADNLAQALDILTPDAVDLSSGVENGVPGQKDLSKITQILHIAQGNTGQRRPPLCDGT

Sequences:

>Translated_222_residues
MRVKICGFTDPGQAQAAARLGVHALGFVCVPGTPRYVDAARLREIAAALPPFTFKVGVFVDAPVEAMRAAVEAGELQGVQ
LHGEESPETCAHLARTLPGILRIKALRVREPADLEKIALYVDAIEAVLLDAWHPTQAGGTGRTLDWKALQGFCPALPWML
SGGLRADNLAQALDILTPDAVDLSSGVENGVPGQKDLSKITQILHIAQGNTGQRRPPLCDGT
>Mature_222_residues
MRVKICGFTDPGQAQAAARLGVHALGFVCVPGTPRYVDAARLREIAAALPPFTFKVGVFVDAPVEAMRAAVEAGELQGVQ
LHGEESPETCAHLARTLPGILRIKALRVREPADLEKIALYVDAIEAVLLDAWHPTQAGGTGRTLDWKALQGFCPALPWML
SGGLRADNLAQALDILTPDAVDLSSGVENGVPGQKDLSKITQILHIAQGNTGQRRPPLCDGT

Specific function: Bifunctional Enzyme That Catalyzes Two Sequential Steps Of Tryptophan Biosynthetic Pathway. The First Reaction Is Catalyzed By The Isomerase, Coded By The Trpf Domain; The Second Reaction Is Catalyzed By The Synthase, Coded By The Trpc Domain. [C]

COG id: COG0135

COG function: function code E; Phosphoribosylanthranilate isomerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpF family

Homologues:

Organism=Escherichia coli, GI87081863, Length=208, Percent_Identity=33.1730769230769, Blast_Score=64, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320210, Length=222, Percent_Identity=29.7297297297297, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRPF_GLOVI (Q7NML1)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_923700.1
- HSSP:   Q56320
- ProteinModelPortal:   Q7NML1
- SMR:   Q7NML1
- GeneID:   2599373
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.754
- HOGENOM:   HBG554803
- OMA:   SKICGIT
- ProtClustDB:   PRK01222
- BioCyc:   GVIO251221:GLL0754-MONOMER
- BRENDA:   5.3.1.24
- HAMAP:   MF_00135_B
- InterPro:   IPR013785
- InterPro:   IPR001240
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF00697 PRAI; SSF51366 RibP_bind_barrel

EC number: =5.3.1.24

Molecular weight: Translated: 23553; Mature: 23553

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVKICGFTDPGQAQAAARLGVHALGFVCVPGTPRYVDAARLREIAAALPPFTFKVGVFV
CEEEEECCCCCCHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEEE
DAPVEAMRAAVEAGELQGVQLHGEESPETCAHLARTLPGILRIKALRVREPADLEKIALY
CCCHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
VDAIEAVLLDAWHPTQAGGTGRTLDWKALQGFCPALPWMLSGGLRADNLAQALDILTPDA
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCC
VDLSSGVENGVPGQKDLSKITQILHIAQGNTGQRRPPLCDGT
CCHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRVKICGFTDPGQAQAAARLGVHALGFVCVPGTPRYVDAARLREIAAALPPFTFKVGVFV
CEEEEECCCCCCHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEEE
DAPVEAMRAAVEAGELQGVQLHGEESPETCAHLARTLPGILRIKALRVREPADLEKIALY
CCCHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
VDAIEAVLLDAWHPTQAGGTGRTLDWKALQGFCPALPWMLSGGLRADNLAQALDILTPDA
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCC
VDLSSGVENGVPGQKDLSKITQILHIAQGNTGQRRPPLCDGT
CCHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292