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The map label for this gene is betT [H]

Identifier: 34499757

GI number: 34499757

Start: 4642503

End: 4644035

Strand: Direct

Name: betT [H]

Synonym: CV_4302

Alternate gene names: 34499757

Gene position: 4642503-4644035 (Clockwise)

Preceding gene: 34499755

Following gene: 34499758

Centisome position: 97.71

GC content: 67.32

Gene sequence:

>1533_bases
ATGGTCCTACGCCTGTCTTTGTTGTTCGCCGTATTGTTCGCGCTCTGGGGCGCCGTCGCCCCCGGACACATGACCGCCGC
CGCCTCGGAGTGGCTGAGCTTCACCATTTCCCGCTTCGGCTGGTTCTACCTGCTGTCGGTGTTCGGCTTCCTGCTGTTCG
CCCTCTACCTGGCTTTCGGCCGCTTCGGCCACATCCGGCTGGGCAAGGAGGACGAAGAGCCGGAATTCTCCCGCCTGAGC
TGGTTTTCCATGCTGTTTTCCGCCGGCATGGGCATCGGCCTGGTGTTCTGGGGCGTGGCCGAGCCGCTGTCCCACTTCGC
CACGCCGGCCAGCGCGGCCACCGCGCCGGAATCGGCAGAAGCCGCGCGGCTGGCGATGCGCCACGCCTTCTTCCACTGGG
GCCTGCACCCATGGGCGGTGTACAGCCTGACCGCGCTGGCGCTGGCCTACTTCAAGTTCAACCGCGGCCAGAAGGGCCTG
ATCAGCGCCGCCTTCCGCCCGCTGCTGGGCGACCGCGTCGACGGCCCGATCGGCGTCGCCATCGACGTGCTGGCGGTGCT
GGCCACCGTGTTCGGCGTCGCCACCACGCTGGGCTTCGGCACGCTGCAGATCGAAAGCGGCCTGGAAATGCTGTTCGGCG
TCACGCCGTCCGCCACGCTGACCGTGGCCATCGTCGTCATCGCCACCGCGCTGTTCCTGCTGTCGTCGCTGACCGGCCTG
TCGCGCGGCATCAAATGGCTGTCCAACCTGAACATCGTGCTGGCGATGCTGCTGTTCCTGGCGGTGGTGATCCTGGGGCC
CACCGGCTTCATCTTCGACACCTTCACCACCACGCTGGGCGATTACCTGGGCAATCTGACCAGCACCAGCCTGCGCATGA
GCCCGTTCACCCAGGGCACCTGGATGGCCAACTGGACGCTGTTCTACTGGGCCTGGTGGGTCACCTGGGCGCCCTTCGTC
GGCATGTTCATCGCCCGCGTCTCGCGCGGCCGCACCGTGCGCGAATTCATCGTCGGCGTGCTCTTGGTGCCGGCGCTGGC
CAGCTTCGTCTGGTTCTCCGCCTTCGGCGGCACCGCGCTGGACCTGCAGCTGTTCGGCGGCGCCGACCTGGTCTCCGCGG
TCAAGGCCGACGTGTCCACCGCGCTGTACGTGATGTTCGAGCAGCTGCCCGGCGGCCGTTTCCTGTCGTTGCTGGCGATG
ACGCTGGTGATCATCTTCTTCGTCACCTCCGCCGATTCCGCCACCTTCGTGCTGGGCATGTTCACCAGCGGCGGCAGCCT
GAACCCCAGCCACCGCGTCAAGCTGATCTGGGGCGTGCTCCTGGCCGCCATCGCGCTGGTGCTGCTGCAGAGCGGCGGCC
TGAAGGCGCTGCAGGCGGTGCTGATCGTCAGCGCGCTGCCCTTCATGCTGATCATGATAGGCATGGCGGTGTCGCTGTAC
CGCGCGCTGAGCGAGGAGGAGCACGACAGCCGGCTGCGCCAGATCCGCCGCCTGCGCCAGTTGGAGGCGCTGGAGAAACG
TCTGCCGGATTGA

Upstream 100 bases:

>100_bases
CATCGCCGGCTTCGCCCGCGGCCCTGCCGCGCCATACGCCACCATCCATCACGGTCGCGCGCCCCGAGCGGGGCGCCTCG
CCGCCATGCAAGGAGTTCCG

Downstream 100 bases:

>100_bases
GCCGGCTTCGGGACGGCAGGCCGATTTGATGCGATGATGACAGCATTGTCTTGCCATGGAGGATGTATGGACACGCACCA
ACTCGACGCGGCGGAAGTGC

Product: high-affinity choline transport protein

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MVLRLSLLFAVLFALWGAVAPGHMTAAASEWLSFTISRFGWFYLLSVFGFLLFALYLAFGRFGHIRLGKEDEEPEFSRLS
WFSMLFSAGMGIGLVFWGVAEPLSHFATPASAATAPESAEAARLAMRHAFFHWGLHPWAVYSLTALALAYFKFNRGQKGL
ISAAFRPLLGDRVDGPIGVAIDVLAVLATVFGVATTLGFGTLQIESGLEMLFGVTPSATLTVAIVVIATALFLLSSLTGL
SRGIKWLSNLNIVLAMLLFLAVVILGPTGFIFDTFTTTLGDYLGNLTSTSLRMSPFTQGTWMANWTLFYWAWWVTWAPFV
GMFIARVSRGRTVREFIVGVLLVPALASFVWFSAFGGTALDLQLFGGADLVSAVKADVSTALYVMFEQLPGGRFLSLLAM
TLVIIFFVTSADSATFVLGMFTSGGSLNPSHRVKLIWGVLLAAIALVLLQSGGLKALQAVLIVSALPFMLIMIGMAVSLY
RALSEEEHDSRLRQIRRLRQLEALEKRLPD

Sequences:

>Translated_510_residues
MVLRLSLLFAVLFALWGAVAPGHMTAAASEWLSFTISRFGWFYLLSVFGFLLFALYLAFGRFGHIRLGKEDEEPEFSRLS
WFSMLFSAGMGIGLVFWGVAEPLSHFATPASAATAPESAEAARLAMRHAFFHWGLHPWAVYSLTALALAYFKFNRGQKGL
ISAAFRPLLGDRVDGPIGVAIDVLAVLATVFGVATTLGFGTLQIESGLEMLFGVTPSATLTVAIVVIATALFLLSSLTGL
SRGIKWLSNLNIVLAMLLFLAVVILGPTGFIFDTFTTTLGDYLGNLTSTSLRMSPFTQGTWMANWTLFYWAWWVTWAPFV
GMFIARVSRGRTVREFIVGVLLVPALASFVWFSAFGGTALDLQLFGGADLVSAVKADVSTALYVMFEQLPGGRFLSLLAM
TLVIIFFVTSADSATFVLGMFTSGGSLNPSHRVKLIWGVLLAAIALVLLQSGGLKALQAVLIVSALPFMLIMIGMAVSLY
RALSEEEHDSRLRQIRRLRQLEALEKRLPD
>Mature_510_residues
MVLRLSLLFAVLFALWGAVAPGHMTAAASEWLSFTISRFGWFYLLSVFGFLLFALYLAFGRFGHIRLGKEDEEPEFSRLS
WFSMLFSAGMGIGLVFWGVAEPLSHFATPASAATAPESAEAARLAMRHAFFHWGLHPWAVYSLTALALAYFKFNRGQKGL
ISAAFRPLLGDRVDGPIGVAIDVLAVLATVFGVATTLGFGTLQIESGLEMLFGVTPSATLTVAIVVIATALFLLSSLTGL
SRGIKWLSNLNIVLAMLLFLAVVILGPTGFIFDTFTTTLGDYLGNLTSTSLRMSPFTQGTWMANWTLFYWAWWVTWAPFV
GMFIARVSRGRTVREFIVGVLLVPALASFVWFSAFGGTALDLQLFGGADLVSAVKADVSTALYVMFEQLPGGRFLSLLAM
TLVIIFFVTSADSATFVLGMFTSGGSLNPSHRVKLIWGVLLAAIALVLLQSGGLKALQAVLIVSALPFMLIMIGMAVSLY
RALSEEEHDSRLRQIRRLRQLEALEKRLPD

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=463, Percent_Identity=38.4449244060475, Blast_Score=342, Evalue=3e-95,
Organism=Escherichia coli, GI1788102, Length=463, Percent_Identity=32.1814254859611, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1786224, Length=463, Percent_Identity=30.0215982721382, Blast_Score=199, Evalue=4e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 55564; Mature: 55564

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS01303 BCCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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HHHCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]