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The map label for this gene is def

Identifier: 34499720

GI number: 34499720

Start: 4599430

End: 4599933

Strand: Reverse

Name: def

Synonym: CV_4265

Alternate gene names: 34499720

Gene position: 4599933-4599430 (Counterclockwise)

Preceding gene: 34499725

Following gene: 34499719

Centisome position: 96.82

GC content: 61.31

Gene sequence:

>504_bases
ATGGCGCTGTTGAATATTTTGCATTACCCGGACGAAAGACTGCACACGGTGGCCAAGCCGGTGGAGGTTTTCGACGCCGC
GTTGCAGCAGCAGATAGACGACATGTTCGAGACCATGTACGAGGCCAAGGGCATCGGCCTGGCGGCCACCCAGGTCGATT
ACCACCGCCGCCTGGTGGTGATGGACATCTCGGAGGAGCGCGACGAGCGCCGGGTGTTCATCAACCCGGAAATCGTCGAG
AAGGACGGCGAGACCGTCTACGAGGAAGGCTGCCTGTCGGTGCCCGGCATCTACGACAAGGTGACCCGCGCCGAGCGCGT
CAAGGTCAAGGCGCAGGACCGCGACGGCAAGCCGTTCGAGCTGGAGGCCGACGGCCTGCTGGCGATCTGCATCCAGCATG
AGCTGGACCATCTGAACGGCGTGGTCTTTGTCGAGCGCCTGTCGCAGATGAAGCAGCAGCGGATCAAGACCAAGCTGAAA
AAGCGCGAAAAGCAGAACATGTGA

Upstream 100 bases:

>100_bases
CGCGCCAGGGGGCGATGCCGGAGAGGAACGAAGCTGCGTCGGACCGCTCTATTATCGACGCGGGCGCGGCAATACGACAA
CTTGGAGTGAGTGGATAGCA

Downstream 100 bases:

>100_bases
TCATCCGGCCGCAGGGTCAGGATTGGGCGGCGCCGCGCGACGGCGCCGCTGTCTTTGTCGGGGACCGAACCAAGATGAAA
TTGATTTTTGCCGGCACGCC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MALLNILHYPDERLHTVAKPVEVFDAALQQQIDDMFETMYEAKGIGLAATQVDYHRRLVVMDISEERDERRVFINPEIVE
KDGETVYEEGCLSVPGIYDKVTRAERVKVKAQDRDGKPFELEADGLLAICIQHELDHLNGVVFVERLSQMKQQRIKTKLK
KREKQNM

Sequences:

>Translated_167_residues
MALLNILHYPDERLHTVAKPVEVFDAALQQQIDDMFETMYEAKGIGLAATQVDYHRRLVVMDISEERDERRVFINPEIVE
KDGETVYEEGCLSVPGIYDKVTRAERVKVKAQDRDGKPFELEADGLLAICIQHELDHLNGVVFVERLSQMKQQRIKTKLK
KREKQNM
>Mature_166_residues
ALLNILHYPDERLHTVAKPVEVFDAALQQQIDDMFETMYEAKGIGLAATQVDYHRRLVVMDISEERDERRVFINPEIVEK
DGETVYEEGCLSVPGIYDKVTRAERVKVKAQDRDGKPFELEADGLLAICIQHELDHLNGVVFVERLSQMKQQRIKTKLKK
REKQNM

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=157, Percent_Identity=30.5732484076433, Blast_Score=73, Evalue=1e-13,
Organism=Escherichia coli, GI1789682, Length=161, Percent_Identity=65.2173913043478, Blast_Score=219, Evalue=5e-59,
Organism=Drosophila melanogaster, GI24645728, Length=145, Percent_Identity=31.0344827586207, Blast_Score=76, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_CHRVO (Q7NQ75)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903935.1
- ProteinModelPortal:   Q7NQ75
- SMR:   Q7NQ75
- GeneID:   2549685
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_4265
- NMPDR:   fig|243365.1.peg.4265
- HOGENOM:   HBG665227
- OMA:   RQLVDDM
- PhylomeDB:   Q7NQ75
- ProtClustDB:   PRK00150
- BioCyc:   CVIO243365:CV_4265-MONOMER
- BRENDA:   3.5.1.88
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 19355; Mature: 19223

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: ACT_SITE 134-134

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLNILHYPDERLHTVAKPVEVFDAALQQQIDDMFETMYEAKGIGLAATQVDYHRRLVV
CCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHCEEEE
MDISEERDERRVFINPEIVEKDGETVYEEGCLSVPGIYDKVTRAERVKVKAQDRDGKPFE
EECCCCCCCCEEEECCHHCCCCCCHHHHCCCCCCCCHHHHHHHHHHEEEEECCCCCCCEE
LEADGLLAICIQHELDHLNGVVFVERLSQMKQQRIKTKLKKREKQNM
ECCCCEEEHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ALLNILHYPDERLHTVAKPVEVFDAALQQQIDDMFETMYEAKGIGLAATQVDYHRRLVV
CHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHCEEEE
MDISEERDERRVFINPEIVEKDGETVYEEGCLSVPGIYDKVTRAERVKVKAQDRDGKPFE
EECCCCCCCCEEEECCHHCCCCCCHHHHCCCCCCCCHHHHHHHHHHEEEEECCCCCCCEE
LEADGLLAICIQHELDHLNGVVFVERLSQMKQQRIKTKLKKREKQNM
ECCCCEEEHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782