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The map label for this gene is fmt

Identifier: 34499719

GI number: 34499719

Start: 4598432

End: 4599355

Strand: Reverse

Name: fmt

Synonym: CV_4264

Alternate gene names: 34499719

Gene position: 4599355-4598432 (Counterclockwise)

Preceding gene: 34499720

Following gene: 34499718

Centisome position: 96.81

GC content: 71.43

Gene sequence:

>924_bases
ATGAAATTGATTTTTGCCGGCACGCCGGAATTCGCCGCCGCCGCGCTGCGCGAGCTGATCGCCGCCGGCCACGAGATCGC
GCTGGTGCTGACCCAGCCGGACCGCCCGGCCGGCCGCGGCATGAAGCTGAAACCGAGCCCGGTGAAGGAAGTGGCGCTGG
CCCACGGCCTGCGCGTCGAGCAGCCGGAGAAGCTGCGCGGCAATCAGGAAGCGCAGCAGATGCTGCGCGACATCCAGGCC
GATGTCATGGTGGTGGCCGCCTACGGACTGATCCTGCCGCAGGACGTTCTGGACATTCCCGCGCGCGGCTGCCTGAACAT
CCACGCGTCCCTGCTGCCGCGCTGGCGCGGCGCGGCGCCGATCCAGCGCGCCATCCTGGCCGGCGATGACGAGACCGGCA
TCACCATCATGCAGATGGACGTCGGCCTCGACACCGGCGACATGCTGAGCATCCACCCGGTGGCGATCGCCGCCGACGAG
ACCGCCGCCACGCTGCACGACAAGCTGGCCGCCTGCGGCGCGCAAGCGATCGTCGAGACATTGGGCCGCCTGGACCGGAT
CGTTCCGCGGAAGCAGCCGGAAGACGGCGTCACCTACGCGCAGAAGCTGTCCAAGGCCGAAGCCGAGGTCGACTGGGCGC
TGCCGGCCGAGCAGGTCGCGCGCGCGATCCGCGCCTACAACCCGGCGCCGGGCGCCTTCACCCAGCTTGGCGGCGAGCCG
CTGAAGCTGTGGATGGCGAGCGCCGAGCCGGGCTCCGCCGAGCCGGGTGCGGTGGTGTCCGCCGACGCCGACGGCGTGCT
GGTAGGCGCAGGGCAAGGGCTGGTGCGCGTGTCCGTATTGCAGGCCGCGGGCGGCAAGCGGCTCGCCGCCCGCGATTTCG
TCGCCGGCAAGAGCCTGCCGGCGGGAACCCGTCTGGGCGTTTGA

Upstream 100 bases:

>100_bases
AAAAGCGCGAAAAGCAGAACATGTGATCATCCGGCCGCAGGGTCAGGATTGGGCGGCGCCGCGCGACGGCGCCGCTGTCT
TTGTCGGGGACCGAACCAAG

Downstream 100 bases:

>100_bases
CGCACTAAGTTTCAGGCAAACCCGCAGTTTCGAGAGGAGAGCATGAACGACAACTGGCTGAAGACCACGATGCTGATGGC
CGCCATCATGGCGCTGTTCG

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MKLIFAGTPEFAAAALRELIAAGHEIALVLTQPDRPAGRGMKLKPSPVKEVALAHGLRVEQPEKLRGNQEAQQMLRDIQA
DVMVVAAYGLILPQDVLDIPARGCLNIHASLLPRWRGAAPIQRAILAGDDETGITIMQMDVGLDTGDMLSIHPVAIAADE
TAATLHDKLAACGAQAIVETLGRLDRIVPRKQPEDGVTYAQKLSKAEAEVDWALPAEQVARAIRAYNPAPGAFTQLGGEP
LKLWMASAEPGSAEPGAVVSADADGVLVGAGQGLVRVSVLQAAGGKRLAARDFVAGKSLPAGTRLGV

Sequences:

>Translated_307_residues
MKLIFAGTPEFAAAALRELIAAGHEIALVLTQPDRPAGRGMKLKPSPVKEVALAHGLRVEQPEKLRGNQEAQQMLRDIQA
DVMVVAAYGLILPQDVLDIPARGCLNIHASLLPRWRGAAPIQRAILAGDDETGITIMQMDVGLDTGDMLSIHPVAIAADE
TAATLHDKLAACGAQAIVETLGRLDRIVPRKQPEDGVTYAQKLSKAEAEVDWALPAEQVARAIRAYNPAPGAFTQLGGEP
LKLWMASAEPGSAEPGAVVSADADGVLVGAGQGLVRVSVLQAAGGKRLAARDFVAGKSLPAGTRLGV
>Mature_307_residues
MKLIFAGTPEFAAAALRELIAAGHEIALVLTQPDRPAGRGMKLKPSPVKEVALAHGLRVEQPEKLRGNQEAQQMLRDIQA
DVMVVAAYGLILPQDVLDIPARGCLNIHASLLPRWRGAAPIQRAILAGDDETGITIMQMDVGLDTGDMLSIHPVAIAADE
TAATLHDKLAACGAQAIVETLGRLDRIVPRKQPEDGVTYAQKLSKAEAEVDWALPAEQVARAIRAYNPAPGAFTQLGGEP
LKLWMASAEPGSAEPGAVVSADADGVLVGAGQGLVRVSVLQAAGGKRLAARDFVAGKSLPAGTRLGV

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI21614513, Length=317, Percent_Identity=28.7066246056782, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI238814322, Length=315, Percent_Identity=29.8412698412698, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI164663775, Length=236, Percent_Identity=35.1694915254237, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1789683, Length=301, Percent_Identity=52.8239202657807, Blast_Score=309, Evalue=2e-85,
Organism=Escherichia coli, GI1788589, Length=311, Percent_Identity=27.6527331189711, Blast_Score=125, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI133930964, Length=303, Percent_Identity=29.3729372937294, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6319458, Length=159, Percent_Identity=32.0754716981132, Blast_Score=66, Evalue=6e-12,
Organism=Drosophila melanogaster, GI28571984, Length=227, Percent_Identity=33.920704845815, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI45550868, Length=227, Percent_Identity=33.920704845815, Blast_Score=114, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24585660, Length=245, Percent_Identity=26.530612244898, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_CHRVO (Q7NQ76)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903934.1
- ProteinModelPortal:   Q7NQ76
- SMR:   Q7NQ76
- GeneID:   2549684
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_4264
- NMPDR:   fig|243365.1.peg.4264
- HOGENOM:   HBG571560
- OMA:   IMQMDEG
- PhylomeDB:   Q7NQ76
- ProtClustDB:   CLSK282782
- BioCyc:   CVIO243365:CV_4264-MONOMER
- BRENDA:   2.1.2.9
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 32115; Mature: 32115

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIFAGTPEFAAAALRELIAAGHEIALVLTQPDRPAGRGMKLKPSPVKEVALAHGLRVE
CEEEECCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCC
QPEKLRGNQEAQQMLRDIQADVMVVAAYGLILPQDVLDIPARGCLNIHASLLPRWRGAAP
CCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHCCCCCCCCH
IQRAILAGDDETGITIMQMDVGLDTGDMLSIHPVAIAADETAATLHDKLAACGAQAIVET
HHHHHHCCCCCCCEEEEEEECCCCCCCEEEECEEEEECCCHHHHHHHHHHHHHHHHHHHH
LGRLDRIVPRKQPEDGVTYAQKLSKAEAEVDWALPAEQVARAIRAYNPAPGAFTQLGGEP
HHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHCCCCE
LKLWMASAEPGSAEPGAVVSADADGVLVGAGQGLVRVSVLQAAGGKRLAARDFVAGKSLP
EEEEEECCCCCCCCCCEEEECCCCEEEEECCCCEEEEEEHHHCCCCEEHHHHHHCCCCCC
AGTRLGV
CCCCCCC
>Mature Secondary Structure
MKLIFAGTPEFAAAALRELIAAGHEIALVLTQPDRPAGRGMKLKPSPVKEVALAHGLRVE
CEEEECCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCC
QPEKLRGNQEAQQMLRDIQADVMVVAAYGLILPQDVLDIPARGCLNIHASLLPRWRGAAP
CCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHCCCCCCCCH
IQRAILAGDDETGITIMQMDVGLDTGDMLSIHPVAIAADETAATLHDKLAACGAQAIVET
HHHHHHCCCCCCCEEEEEEECCCCCCCEEEECEEEEECCCHHHHHHHHHHHHHHHHHHHH
LGRLDRIVPRKQPEDGVTYAQKLSKAEAEVDWALPAEQVARAIRAYNPAPGAFTQLGGEP
HHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHCCCCE
LKLWMASAEPGSAEPGAVVSADADGVLVGAGQGLVRVSVLQAAGGKRLAARDFVAGKSLP
EEEEEECCCCCCCCCCEEEECCCCEEEEECCCCEEEEEEHHHCCCCEEHHHHHHCCCCCC
AGTRLGV
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782