Click here to switch to the map view.

The map label for this gene is moeB [H]

Identifier: 34499713

GI number: 34499713

Start: 4591378

End: 4592139

Strand: Reverse

Name: moeB [H]

Synonym: CV_4258

Alternate gene names: 34499713

Gene position: 4592139-4591378 (Counterclockwise)

Preceding gene: 34499714

Following gene: 34499706

Centisome position: 96.65

GC content: 71.26

Gene sequence:

>762_bases
ATGGAAGACCGCGAACTGGACGACGAGGCGCTGCTGCGCTACAGCCGCCACATCCTGTTGCCGGAGATCGACATCGCCGG
CCAGCGCCGGCTGCTGGCGGCGCGCGCGCTGATCGTCGGCGCCGGCGGCCTGGGCTCGCCGGCGGCGCTGTACCTGGCCA
GCGCCGGCGTCGGCCGCATCACCATCGTCGACGACGACGCGGTGGAACTGTCCAATCTGCAGCGCCAGATCGCCCACGAT
ACCGCCAGCCTGGGCCAGGGCAAGGCGGCATCCGCCGCGCGGCGCATGCTGGCCTTGAATCCCACCATAGAGGCCAGGCC
GCTGGCCGAGCGGCTATCTGGCCAGAGATTGATGGACGAAGTTGCCGCCCACGACCTGGTGCTGGATTGCTCGGACAATT
TCGCCACCCGCCACGCGGTCAACCGCGCCTGCGTGGCCGCCAGGGTGCCGCTGGTGTCCGGCGCGGCGGTGCGCTTCTCC
GGCCAGCTGGCGGTGTTCGACAGCCGCGACGCGGCCTCGCCGTGCTACCACTGCCTGTTTCCGGACGAGGGCGAGGCCAG
CGACGGCCCCTGCGCCACCTTCGGCGTGCTGTCGCCCTTGGTGGGCGCGATAGGCAGCCTGCAAGCGGTGGAGGCGGTCA
AGCTGCTGGCCGGCGTCCCGCCGGCGCTGGGCAAGCTGACCCTGTACGACGCGCTGGACGGAAGCTTCAAGCAGATCCGC
GTGCCGCGCGATCCTGCTTGCGCCGTATGCGGGGGCGCATGA

Upstream 100 bases:

>100_bases
TATTTGCGCAGGCGCGCCTCGTCTCAGGCGTTTTGCTCGACACAAATCGCTTATCGACGCCAGCCTTTCCGGCTGGCGTT
TCGCATTCTGGAGCATGAGC

Downstream 100 bases:

>100_bases
ACTTGCGATCTGGTCAGGGCCGTAGGGCGGAATCCCGGCTTTTAGCCGGGAGTTCCGCCGAGGTAGCTAGGTTAGGCGGG
CGGCGGAACGCCCCAAGGGG

Product: molybdopterin biosynthesis MoeB protein

Products: NA

Alternate protein names: MoaD protein adenylase; Molybdopterin-converting factor subunit 1 adenylase [H]

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MEDRELDDEALLRYSRHILLPEIDIAGQRRLLAARALIVGAGGLGSPAALYLASAGVGRITIVDDDAVELSNLQRQIAHD
TASLGQGKAASAARRMLALNPTIEARPLAERLSGQRLMDEVAAHDLVLDCSDNFATRHAVNRACVAARVPLVSGAAVRFS
GQLAVFDSRDAASPCYHCLFPDEGEASDGPCATFGVLSPLVGAIGSLQAVEAVKLLAGVPPALGKLTLYDALDGSFKQIR
VPRDPACAVCGGA

Sequences:

>Translated_253_residues
MEDRELDDEALLRYSRHILLPEIDIAGQRRLLAARALIVGAGGLGSPAALYLASAGVGRITIVDDDAVELSNLQRQIAHD
TASLGQGKAASAARRMLALNPTIEARPLAERLSGQRLMDEVAAHDLVLDCSDNFATRHAVNRACVAARVPLVSGAAVRFS
GQLAVFDSRDAASPCYHCLFPDEGEASDGPCATFGVLSPLVGAIGSLQAVEAVKLLAGVPPALGKLTLYDALDGSFKQIR
VPRDPACAVCGGA
>Mature_253_residues
MEDRELDDEALLRYSRHILLPEIDIAGQRRLLAARALIVGAGGLGSPAALYLASAGVGRITIVDDDAVELSNLQRQIAHD
TASLGQGKAASAARRMLALNPTIEARPLAERLSGQRLMDEVAAHDLVLDCSDNFATRHAVNRACVAARVPLVSGAAVRFS
GQLAVFDSRDAASPCYHCLFPDEGEASDGPCATFGVLSPLVGAIGSLQAVEAVKLLAGVPPALGKLTLYDALDGSFKQIR
VPRDPACAVCGGA

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein moaD [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=249, Percent_Identity=47.7911646586345, Blast_Score=184, Evalue=7e-47,
Organism=Homo sapiens, GI4885649, Length=145, Percent_Identity=31.0344827586207, Blast_Score=75, Evalue=7e-14,
Organism=Escherichia coli, GI1787048, Length=248, Percent_Identity=50, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI87082356, Length=248, Percent_Identity=46.7741935483871, Blast_Score=211, Evalue=5e-56,
Organism=Caenorhabditis elegans, GI17540406, Length=242, Percent_Identity=42.5619834710744, Blast_Score=192, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI193203301, Length=153, Percent_Identity=30.0653594771242, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6321903, Length=219, Percent_Identity=37.8995433789954, Blast_Score=152, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6322639, Length=171, Percent_Identity=27.4853801169591, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24582879, Length=250, Percent_Identity=42, Blast_Score=186, Evalue=9e-48,
Organism=Drosophila melanogaster, GI24660640, Length=157, Percent_Identity=31.2101910828025, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR012730
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 26403; Mature: 26403

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDRELDDEALLRYSRHILLPEIDIAGQRRLLAARALIVGAGGLGSPAALYLASAGVGRI
CCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHEEECCCCCCHHHHHHCCCCCCEE
TIVDDDAVELSNLQRQIAHDTASLGQGKAASAARRMLALNPTIEARPLAERLSGQRLMDE
EEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHH
VAAHDLVLDCSDNFATRHAVNRACVAARVPLVSGAAVRFSGQLAVFDSRDAASPCYHCLF
HHHHHEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECEEEEEECCCCCCCEEEEEC
PDEGEASDGPCATFGVLSPLVGAIGSLQAVEAVKLLAGVPPALGKLTLYDALDGSFKQIR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCEEEEHHCCCCHHEEE
VPRDPACAVCGGA
CCCCCCCCCCCCC
>Mature Secondary Structure
MEDRELDDEALLRYSRHILLPEIDIAGQRRLLAARALIVGAGGLGSPAALYLASAGVGRI
CCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHEEECCCCCCHHHHHHCCCCCCEE
TIVDDDAVELSNLQRQIAHDTASLGQGKAASAARRMLALNPTIEARPLAERLSGQRLMDE
EEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHH
VAAHDLVLDCSDNFATRHAVNRACVAARVPLVSGAAVRFSGQLAVFDSRDAASPCYHCLF
HHHHHEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECEEEEEECCCCCCCEEEEEC
PDEGEASDGPCATFGVLSPLVGAIGSLQAVEAVKLLAGVPPALGKLTLYDALDGSFKQIR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCEEEEHHCCCCHHEEE
VPRDPACAVCGGA
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3045084; 8905232; 9278503 [H]