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The map label for this gene is ntrX [H]

Identifier: 34499714

GI number: 34499714

Start: 4592280

End: 4593548

Strand: Reverse

Name: ntrX [H]

Synonym: CV_4259

Alternate gene names: 34499714

Gene position: 4593548-4592280 (Counterclockwise)

Preceding gene: 34499715

Following gene: 34499713

Centisome position: 96.68

GC content: 64.54

Gene sequence:

>1269_bases
ATGCGTAGCAGCGATATTTTGATTGTGGACGACGAGATCGGCATCCGAGAACTGCTCTCCGAGATCCTGCAGGATGAAGG
TTATACCGTGGCCCTGGCGGAAAACGCCGAAGTGGCCCGCCAATTGCGCAACCAGACCCGGCCGGCGCTGGTGCTGCTGG
ACATCTGGATGCCCGACTGCGACGGCGTCACCCTGCTGAAGGAATGGGCCAAGTCCGGCCTGCTCAACATGCCGGTGGTG
ATGATGTCCGGCCACGCCAGCATAGACACCGCGGTGGAGGCCACCCGCATCGGCGCCTTCGACTTCCTGGAAAAGCCGAT
CGCGCTGCAGAAGCTGCTCACCACCGTTCAGCGCGCGCTGAAATACGGCGACATGCAGGCCAACACTTCGCTGAACCTGG
ACAAGCTGGGCCGCAGCGAGCCCATCCAGGAGCTGAAGCGCCAGCTCGAGCGCGTGGCCAAGGTCAAGTCGCCGGTGCTG
CTGACCGGCGAGTCTGGCTCCGGCTTCGAGCTGGTGGCGCGCTTCTTCCACCAGGGCAACACGCCGTGGGTGACGCCGGC
CAAGCTGGAGCAACTGGCCGACGCGCCGCTTGAGCTGCTGCAGAAGGCCAACAACGGCGTGCTCTACCTGCCGGAGATCG
GCCAGTACAGCCGCCGCGTGCAGCAGGGCCTGCTGTTCCTGCTGTCCAAGCTCGACCGCTTCAACGTGCGCCTGATCTGT
TCCTGCAGCCGCCCGCTGCAGGAGCTGCTGGTGGACCCCGAATGCGACAACCGGCTGCTGACCGCGCTGTCCAGCGTGAT
CGTGCCGATTCCGCCGCTGCGCGAGCACGCCGAGGACATCACCTTCATCGCCGAGCAGATCCTGGTGGAGCTGGTGGAGT
CCAAGCAGGTGCCGGCGCGCAAGCTGACCACCGCGGCGCTGAACGCGATGCGCCAGTACGACTGGCCGGGCAACCTGGAA
CAGCTGCGCAGCATCATCAAGAGCCTGGCGCTGACCGCCGAGTCCGACGAAGTGGACGCCGCCGAGGTCAACAAGGTGCT
GGCCCAGTTCCGCCACGAGAAGCCGGTGGAGGAGTCGGGCTTCGATTTCAACATCCCGCTGCGCGAACTGCGCGAGCAGC
TGGAGCGCCGCTATTTCGAGTACCACATCTCGCTGGAAAACGGCAATATGAGCCGGGTGGCGCAGAAGGTAGGCCTGGAG
CGCACGCACTTGTACCGCAAACTGAAGCAGTTGGGCATCAAGTTCGCCCGCAAGGTGGCGGAAGAGTAA

Upstream 100 bases:

>100_bases
TCAAGAAGATTATGGAAGAACACCATGGACAGGTCATTTTGGGGAACGCCGAGCAGCAAGGCGCGCGTGTCCTTCTCTCC
CTGCCATTGCTGGAGGCCTA

Downstream 100 bases:

>100_bases
GACAAAACGCCTGATACGGCGTTGCGCCTCCTCGCCGTACTATTTGCGCAGGCGCGCCTCGTCTCAGGCGTTTTGCTCGA
CACAAATCGCTTATCGACGC

Product: nitrogen assimilation regulatory protein NtrX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MRSSDILIVDDEIGIRELLSEILQDEGYTVALAENAEVARQLRNQTRPALVLLDIWMPDCDGVTLLKEWAKSGLLNMPVV
MMSGHASIDTAVEATRIGAFDFLEKPIALQKLLTTVQRALKYGDMQANTSLNLDKLGRSEPIQELKRQLERVAKVKSPVL
LTGESGSGFELVARFFHQGNTPWVTPAKLEQLADAPLELLQKANNGVLYLPEIGQYSRRVQQGLLFLLSKLDRFNVRLIC
SCSRPLQELLVDPECDNRLLTALSSVIVPIPPLREHAEDITFIAEQILVELVESKQVPARKLTTAALNAMRQYDWPGNLE
QLRSIIKSLALTAESDEVDAAEVNKVLAQFRHEKPVEESGFDFNIPLRELREQLERRYFEYHISLENGNMSRVAQKVGLE
RTHLYRKLKQLGIKFARKVAEE

Sequences:

>Translated_422_residues
MRSSDILIVDDEIGIRELLSEILQDEGYTVALAENAEVARQLRNQTRPALVLLDIWMPDCDGVTLLKEWAKSGLLNMPVV
MMSGHASIDTAVEATRIGAFDFLEKPIALQKLLTTVQRALKYGDMQANTSLNLDKLGRSEPIQELKRQLERVAKVKSPVL
LTGESGSGFELVARFFHQGNTPWVTPAKLEQLADAPLELLQKANNGVLYLPEIGQYSRRVQQGLLFLLSKLDRFNVRLIC
SCSRPLQELLVDPECDNRLLTALSSVIVPIPPLREHAEDITFIAEQILVELVESKQVPARKLTTAALNAMRQYDWPGNLE
QLRSIIKSLALTAESDEVDAAEVNKVLAQFRHEKPVEESGFDFNIPLRELREQLERRYFEYHISLENGNMSRVAQKVGLE
RTHLYRKLKQLGIKFARKVAEE
>Mature_422_residues
MRSSDILIVDDEIGIRELLSEILQDEGYTVALAENAEVARQLRNQTRPALVLLDIWMPDCDGVTLLKEWAKSGLLNMPVV
MMSGHASIDTAVEATRIGAFDFLEKPIALQKLLTTVQRALKYGDMQANTSLNLDKLGRSEPIQELKRQLERVAKVKSPVL
LTGESGSGFELVARFFHQGNTPWVTPAKLEQLADAPLELLQKANNGVLYLPEIGQYSRRVQQGLLFLLSKLDRFNVRLIC
SCSRPLQELLVDPECDNRLLTALSSVIVPIPPLREHAEDITFIAEQILVELVESKQVPARKLTTAALNAMRQYDWPGNLE
QLRSIIKSLALTAESDEVDAAEVNKVLAQFRHEKPVEESGFDFNIPLRELREQLERRYFEYHISLENGNMSRVAQKVGLE
RTHLYRKLKQLGIKFARKVAEE

Specific function: Member of the two-component regulatory system ntrY/ntrX involved in the activation of nitrogen assimilatory genes such as glnA. NtrX is probably phosphorylated by ntrY and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=456, Percent_Identity=29.6052631578947, Blast_Score=171, Evalue=6e-44,
Organism=Escherichia coli, GI1790437, Length=447, Percent_Identity=29.3064876957494, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1790299, Length=475, Percent_Identity=29.0526315789474, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1788905, Length=443, Percent_Identity=28.8939051918736, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI87082117, Length=346, Percent_Identity=24.8554913294798, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081872, Length=316, Percent_Identity=26.2658227848101, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1789233, Length=322, Percent_Identity=25.1552795031056, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI87082152, Length=316, Percent_Identity=25.6329113924051, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1789087, Length=322, Percent_Identity=26.3975155279503, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1787583, Length=311, Percent_Identity=24.4372990353698, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1786838, Length=152, Percent_Identity=32.2368421052632, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI87081858, Length=304, Percent_Identity=24.6710526315789, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787375, Length=128, Percent_Identity=27.34375, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786599, Length=117, Percent_Identity=28.2051282051282, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786784, Length=118, Percent_Identity=32.2033898305085, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 47687; Mature: 47687

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSSDILIVDDEIGIRELLSEILQDEGYTVALAENAEVARQLRNQTRPALVLLDIWMPDC
CCCCCEEEECCCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCCEEEEEEECCCCC
DGVTLLKEWAKSGLLNMPVVMMSGHASIDTAVEATRIGAFDFLEKPIALQKLLTTVQRAL
CCHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHCCHHHHHHCHHHHHHHHHHHHHHH
KYGDMQANTSLNLDKLGRSEPIQELKRQLERVAKVKSPVLLTGESGSGFELVARFFHQGN
HHCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHCCC
TPWVTPAKLEQLADAPLELLQKANNGVLYLPEIGQYSRRVQQGLLFLLSKLDRFNVRLIC
CCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEEE
SCSRPLQELLVDPECDNRLLTALSSVIVPIPPLREHAEDITFIAEQILVELVESKQVPAR
ECCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHH
KLTTAALNAMRQYDWPGNLEQLRSIIKSLALTAESDEVDAAEVNKVLAQFRHEKPVEESG
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
FDFNIPLRELREQLERRYFEYHISLENGNMSRVAQKVGLERTHLYRKLKQLGIKFARKVA
CCCCCCHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
EE
CC
>Mature Secondary Structure
MRSSDILIVDDEIGIRELLSEILQDEGYTVALAENAEVARQLRNQTRPALVLLDIWMPDC
CCCCCEEEECCCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCCEEEEEEECCCCC
DGVTLLKEWAKSGLLNMPVVMMSGHASIDTAVEATRIGAFDFLEKPIALQKLLTTVQRAL
CCHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHCCHHHHHHCHHHHHHHHHHHHHHH
KYGDMQANTSLNLDKLGRSEPIQELKRQLERVAKVKSPVLLTGESGSGFELVARFFHQGN
HHCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHCCC
TPWVTPAKLEQLADAPLELLQKANNGVLYLPEIGQYSRRVQQGLLFLLSKLDRFNVRLIC
CCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEEE
SCSRPLQELLVDPECDNRLLTALSSVIVPIPPLREHAEDITFIAEQILVELVESKQVPAR
ECCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHH
KLTTAALNAMRQYDWPGNLEQLRSIIKSLALTAESDEVDAAEVNKVLAQFRHEKPVEESG
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
FDFNIPLRELREQLERRYFEYHISLENGNMSRVAQKVGLERTHLYRKLKQLGIKFARKVA
CCCCCCHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
EE
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]