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The map label for this gene is ruvC

Identifier: 34499680

GI number: 34499680

Start: 4559440

End: 4559970

Strand: Reverse

Name: ruvC

Synonym: CV_4225

Alternate gene names: 34499680

Gene position: 4559970-4559440 (Counterclockwise)

Preceding gene: 34499681

Following gene: 34499678

Centisome position: 95.98

GC content: 67.8

Gene sequence:

>531_bases
ATGAGCCGGCGCATCTTAGGCATCGATCCCGGCAGCCTGATCACCGGCTTCGGCGTCATCGACGTGGTGGGCAACCAGCG
CCATTACGTCGCTTCCGGCGCGATCCGCACCAAGAGCGGATCGCCGCTGGCCGAGCGGGTCAAGGTGCTGGTGGACGGCA
TCCATCAGGTGATAGACACCTATCGGCCCACCGAGGCGGCGCTGGAACGGGTGTTCGTCAACGTCAATCCGGCGGCCACG
CTGATGCTGGGACAGGCGCGCGGCGCCTGCATGACCGCGCTGGTGCTGAAGGATTTGCCGGTGGCCGAATACACGGCGCT
GCAGGTCAAGCAATCCGTGGTCGGTCAGGGCAAGGCGCCGAAGGAGCAGGTGCAGTACATGGTGGTGCGCATGCTGAACC
TGTCCGGCACGCCGCAGGCCGACGCCGCCGACGCGCTGGCGGTGGCGCTGACCCATGCGAATCATAGCGGCGGGGCCATC
GGCAAGCTGGCGCTGCGGGGCCTGAAGGTGCGGGGCGGCCGCTTGGTGTAG

Upstream 100 bases:

>100_bases
GGGGCCGGATCCGCGGCGAGGTGCAGACCGGCTTGTGGCTGGACGTCGGCACGCCGGAGCGGCTGGCCGAAGCCGGCCGC
ATCGCCTCCGGCTGGACGAG

Downstream 100 bases:

>100_bases
GACCTCGGCCAGGCGGTTGTGGCCGCCGTCCGCAACAAAGCGGCCCGCGGGCCGCTTTGTTGCCTTTTCCGAACGCGTCA
GCGCCGCCGGGCATCCGGCC

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 176; Mature: 175

Protein sequence:

>176_residues
MSRRILGIDPGSLITGFGVIDVVGNQRHYVASGAIRTKSGSPLAERVKVLVDGIHQVIDTYRPTEAALERVFVNVNPAAT
LMLGQARGACMTALVLKDLPVAEYTALQVKQSVVGQGKAPKEQVQYMVVRMLNLSGTPQADAADALAVALTHANHSGGAI
GKLALRGLKVRGGRLV

Sequences:

>Translated_176_residues
MSRRILGIDPGSLITGFGVIDVVGNQRHYVASGAIRTKSGSPLAERVKVLVDGIHQVIDTYRPTEAALERVFVNVNPAAT
LMLGQARGACMTALVLKDLPVAEYTALQVKQSVVGQGKAPKEQVQYMVVRMLNLSGTPQADAADALAVALTHANHSGGAI
GKLALRGLKVRGGRLV
>Mature_175_residues
SRRILGIDPGSLITGFGVIDVVGNQRHYVASGAIRTKSGSPLAERVKVLVDGIHQVIDTYRPTEAALERVFVNVNPAATL
MLGQARGACMTALVLKDLPVAEYTALQVKQSVVGQGKAPKEQVQYMVVRMLNLSGTPQADAADALAVALTHANHSGGAIG
KLALRGLKVRGGRLV

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=171, Percent_Identity=49.7076023391813, Blast_Score=130, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_CHRVO (Q7NQB5)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903895.1
- ProteinModelPortal:   Q7NQB5
- SMR:   Q7NQB5
- GeneID:   2549537
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_4225
- NMPDR:   fig|243365.1.peg.4225
- HOGENOM:   HBG437552
- OMA:   DCAAIEK
- PhylomeDB:   Q7NQB5
- ProtClustDB:   PRK00039
- BioCyc:   CVIO243365:CV_4225-MONOMER
- BRENDA:   3.1.22.4
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 18518; Mature: 18387

Theoretical pI: Translated: 10.56; Mature: 10.56

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRILGIDPGSLITGFGVIDVVGNQRHYVASGAIRTKSGSPLAERVKVLVDGIHQVIDT
CCCCEECCCCCCHHHHCHHEEEECCCCEEEECCCEECCCCCCHHHHHHHHHHHHHHHHHC
YRPTEAALERVFVNVNPAATLMLGQARGACMTALVLKDLPVAEYTALQVKQSVVGQGKAP
CCCHHHHHHHHEECCCCHHEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCC
KEQVQYMVVRMLNLSGTPQADAADALAVALTHANHSGGAIGKLALRGLKVRGGRLV
HHHHHHHHHHHHCCCCCCCCCHHHHHEEEEEECCCCCCHHHHHHHHCEECCCCCCC
>Mature Secondary Structure 
SRRILGIDPGSLITGFGVIDVVGNQRHYVASGAIRTKSGSPLAERVKVLVDGIHQVIDT
CCCEECCCCCCHHHHCHHEEEECCCCEEEECCCEECCCCCCHHHHHHHHHHHHHHHHHC
YRPTEAALERVFVNVNPAATLMLGQARGACMTALVLKDLPVAEYTALQVKQSVVGQGKAP
CCCHHHHHHHHEECCCCHHEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCC
KEQVQYMVVRMLNLSGTPQADAADALAVALTHANHSGGAIGKLALRGLKVRGGRLV
HHHHHHHHHHHHCCCCCCCCCHHHHHEEEEEECCCCCCHHHHHHHHCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782