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The map label for this gene is ruvA

Identifier: 34499678

GI number: 34499678

Start: 4557480

End: 4558073

Strand: Reverse

Name: ruvA

Synonym: CV_4223

Alternate gene names: 34499678

Gene position: 4558073-4557480 (Counterclockwise)

Preceding gene: 34499680

Following gene: 34499677

Centisome position: 95.94

GC content: 65.66

Gene sequence:

>594_bases
ATGATCGGACGTCTTTCCGGAAAACTGATCGAAAAATTGCCGCCGCAAGTGGTGGTGGATGTGAACGGCGTCGGCTACGA
AGTGGACGTGCCGATGACCACCTTCTACCAGTTGCCGGCGCTGGGACAGCCCTGCACCTTGTTCACCCACCTGGCGGTGC
GCGAGGACGCCCATTTGCTGTACGGCTTCGCCAGCAAGGAAGAGAGACAGACTTTCCGCCAGCTGATCAAGGTGACCGGC
ATCGGCGCCAAGATCGCGCTGGCCATCCTGTCCGGCATGACCGCCGACGAGCTGGCGATCGCGGTGGCCAGCGAGGACAT
CAAGCGGCTGTCGGCGGTGCCGGGCATCGGCAAGAAGACCGCCGAGCGGCTGGTGCTGGAGCTGCGCGGCAAGCTGGCCA
CCGGCGGCAACCTGACGGTGCCGGGCGGACTGCCTTTCGCCGCCACGCCGGACGAGAAGAGCGACATCGTCAACGCGCTG
CTGGCGCTGGGCTACAACGAGAAGGAGGCGGCGGCGGCCACCAAGAGCCTGCCGGCCGATGTGACCGTCAGCGAGGGCGT
GCGCCTGGCCCTGAAGAGCCTGATGAAGGTTTGA

Upstream 100 bases:

>100_bases
CTGGCCTTGCCGGACAAGCTGGATGGGCGCGGGCGCGGCTTGTGGGATAATGCCGGCAGCCGCGCCAGTCCGGCGCGGGC
CTGATTGCGAGAATGCCAAG

Downstream 100 bases:

>100_bases
TGCCTATACTGTCGATGCGGCTTGTATATCGCTGCATCGACTAGGGAGCGGACATGGCGTGGCGATGCGCGGCGGGGGCG
TTGGCGGCGAGCCTGGGAAT

Product: Holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MIGRLSGKLIEKLPPQVVVDVNGVGYEVDVPMTTFYQLPALGQPCTLFTHLAVREDAHLLYGFASKEERQTFRQLIKVTG
IGAKIALAILSGMTADELAIAVASEDIKRLSAVPGIGKKTAERLVLELRGKLATGGNLTVPGGLPFAATPDEKSDIVNAL
LALGYNEKEAAAATKSLPADVTVSEGVRLALKSLMKV

Sequences:

>Translated_197_residues
MIGRLSGKLIEKLPPQVVVDVNGVGYEVDVPMTTFYQLPALGQPCTLFTHLAVREDAHLLYGFASKEERQTFRQLIKVTG
IGAKIALAILSGMTADELAIAVASEDIKRLSAVPGIGKKTAERLVLELRGKLATGGNLTVPGGLPFAATPDEKSDIVNAL
LALGYNEKEAAAATKSLPADVTVSEGVRLALKSLMKV
>Mature_197_residues
MIGRLSGKLIEKLPPQVVVDVNGVGYEVDVPMTTFYQLPALGQPCTLFTHLAVREDAHLLYGFASKEERQTFRQLIKVTG
IGAKIALAILSGMTADELAIAVASEDIKRLSAVPGIGKKTAERLVLELRGKLATGGNLTVPGGLPFAATPDEKSDIVNAL
LALGYNEKEAAAATKSLPADVTVSEGVRLALKSLMKV

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family

Homologues:

Organism=Escherichia coli, GI1788168, Length=203, Percent_Identity=47.2906403940887, Blast_Score=177, Evalue=4e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVA_CHRVO (Q7NQB7)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903893.1
- ProteinModelPortal:   Q7NQB7
- SMR:   Q7NQB7
- GeneID:   2549535
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_4223
- NMPDR:   fig|243365.1.peg.4223
- HOGENOM:   HBG635309
- OMA:   LSIETYV
- PhylomeDB:   Q7NQB7
- ProtClustDB:   PRK00116
- BioCyc:   CVIO243365:CV_4223-MONOMER
- HAMAP:   MF_00031
- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278
- TIGRFAMs:   TIGR00084

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like; SSF46929 RuvA_C-like

EC number: =3.6.4.12

Molecular weight: Translated: 20823; Mature: 20823

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGRLSGKLIEKLPPQVVVDVNGVGYEVDVPMTTFYQLPALGQPCTLFTHLAVREDAHLL
CCCCCCCHHHHHCCCEEEEEECCCCEEECCCHHHHHHCCCCCCCHHHHHHHHHHCCCCEE
YGFASKEERQTFRQLIKVTGIGAKIALAILSGMTADELAIAVASEDIKRLSAVPGIGKKT
EECCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHH
AERLVLELRGKLATGGNLTVPGGLPFAATPDEKSDIVNALLALGYNEKEAAAATKSLPAD
HHHHHHHHHCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
VTVSEGVRLALKSLMKV
CCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIGRLSGKLIEKLPPQVVVDVNGVGYEVDVPMTTFYQLPALGQPCTLFTHLAVREDAHLL
CCCCCCCHHHHHCCCEEEEEECCCCEEECCCHHHHHHCCCCCCCHHHHHHHHHHCCCCEE
YGFASKEERQTFRQLIKVTGIGAKIALAILSGMTADELAIAVASEDIKRLSAVPGIGKKT
EECCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHH
AERLVLELRGKLATGGNLTVPGGLPFAATPDEKSDIVNALLALGYNEKEAAAATKSLPAD
HHHHHHHHHCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
VTVSEGVRLALKSLMKV
CCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782