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The map label for this gene is mutM

Identifier: 34499517

GI number: 34499517

Start: 4392630

End: 4393442

Strand: Direct

Name: mutM

Synonym: CV_4062

Alternate gene names: 34499517

Gene position: 4392630-4393442 (Clockwise)

Preceding gene: 34499506

Following gene: 34499518

Centisome position: 92.46

GC content: 69.37

Gene sequence:

>813_bases
ATGCCTGAACTGCCAGAAGTCGAAACTACCCGCCGCGGCGTCGAGCCGCACCTGGAAGGCCGAACCCTGCTCGGCGCCGT
CGTCCGCAATCCCAGCCTGCGCTGGCCGGTGCCGCCCGACCTGTCCGAGCGCGTCGCCGGGGAAAAGGTGCTGGCCGTGC
GCCGCCGCGCCAAATACCTGCTGCTGGAATGCGAAAGCGGCACCCTGCTGATCCACCTGGGCATGTCCGGCAGCCTGAGG
GTGATGCCGGCCGGCGCGCCGCCGCAAAAACACGATCACCTGGACTTGCTGTTGGGCGAACAGGTGCTGCGCTTCCGCGA
TCCGCGGCGCTTCGGCGCGGTGCTGTGGCATCTCGGCCCCGTCGAGATGCACCCGCTGCTGCAGGCGCTGGGGCCGGAGC
CGCTGTCCGACGCCTTCGACGGCGCGGCGCTCCATCAGGCCATCCGCCGCCGCGGCAGTCCGATCAAGCTGGCCATCATG
GACAACCACGTGGTGGTCGGCGTCGGCAACATCTACGCCAACGAATCGCTGTTCCACGCCGGCATCAGTCCCGCCCGCGC
GGCCTGCGATCTGTCCCGCGCCGACTGCGACCGCCTCGCGGCCGAGATCAAGGCCGTGCTGCGGCGCGCGATCGACGCCG
GCGGCAGCACGCTGCGCGATTTCGTCGACAGCGAGGGCAAACCCGGCTACTTCCAGCAGACCTATATGGTGTACAACCGC
CAGGAAGAGCCCTGCCGCCTCTGCGGCACGCCGATCCGCCAAATCCGCCAGGGCCAGCGCAGCACTTACTATTGCCCGCT
GTGCCAGCCGTGA

Upstream 100 bases:

>100_bases
TCATTCGCATGGATTAAGTTGCCTGCTCAAAGCCTGTTGAACGCGTAGAATAGCATGACCGGTTTTCATCTTTGCACCAT
TTACCAAATCCGCTCCGCCC

Downstream 100 bases:

>100_bases
TAGAGTGGCGGCCTGTCATCCCTCAGACTTGAAACCGTAACCATCTTGAAGTCACACGCCACCACTACATTCGCCACCCG
CTGCGGCCGCGTGACGCGCC

Product: formamidopyrimidine-DNA glycosylase

Products: NA

Alternate protein names: Fapy-DNA glycosylase; DNA-(apurinic or apyrimidinic site) lyase mutM; AP lyase mutM

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MPELPEVETTRRGVEPHLEGRTLLGAVVRNPSLRWPVPPDLSERVAGEKVLAVRRRAKYLLLECESGTLLIHLGMSGSLR
VMPAGAPPQKHDHLDLLLGEQVLRFRDPRRFGAVLWHLGPVEMHPLLQALGPEPLSDAFDGAALHQAIRRRGSPIKLAIM
DNHVVVGVGNIYANESLFHAGISPARAACDLSRADCDRLAAEIKAVLRRAIDAGGSTLRDFVDSEGKPGYFQQTYMVYNR
QEEPCRLCGTPIRQIRQGQRSTYYCPLCQP

Sequences:

>Translated_270_residues
MPELPEVETTRRGVEPHLEGRTLLGAVVRNPSLRWPVPPDLSERVAGEKVLAVRRRAKYLLLECESGTLLIHLGMSGSLR
VMPAGAPPQKHDHLDLLLGEQVLRFRDPRRFGAVLWHLGPVEMHPLLQALGPEPLSDAFDGAALHQAIRRRGSPIKLAIM
DNHVVVGVGNIYANESLFHAGISPARAACDLSRADCDRLAAEIKAVLRRAIDAGGSTLRDFVDSEGKPGYFQQTYMVYNR
QEEPCRLCGTPIRQIRQGQRSTYYCPLCQP
>Mature_269_residues
PELPEVETTRRGVEPHLEGRTLLGAVVRNPSLRWPVPPDLSERVAGEKVLAVRRRAKYLLLECESGTLLIHLGMSGSLRV
MPAGAPPQKHDHLDLLLGEQVLRFRDPRRFGAVLWHLGPVEMHPLLQALGPEPLSDAFDGAALHQAIRRRGSPIKLAIMD
NHVVVGVGNIYANESLFHAGISPARAACDLSRADCDRLAAEIKAVLRRAIDAGGSTLRDFVDSEGKPGYFQQTYMVYNRQ
EEPCRLCGTPIRQIRQGQRSTYYCPLCQP

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyr

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger

Homologues:

Organism=Escherichia coli, GI1790066, Length=270, Percent_Identity=59.2592592592593, Blast_Score=319, Evalue=1e-88,
Organism=Escherichia coli, GI1786932, Length=282, Percent_Identity=26.241134751773, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FPG_CHRVO (Q7NQS5)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903732.1
- ProteinModelPortal:   Q7NQS5
- SMR:   Q7NQS5
- GeneID:   2551231
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_4062
- NMPDR:   fig|243365.1.peg.4062
- HOGENOM:   HBG690070
- OMA:   RSTFYCA
- PhylomeDB:   Q7NQS5
- ProtClustDB:   PRK01103
- BioCyc:   CVIO243365:CV_4062-MONOMER
- BRENDA:   3.2.2.23
- BRENDA:   4.2.99.18
- HAMAP:   MF_00103
- InterPro:   IPR015886
- InterPro:   IPR015887
- InterPro:   IPR000191
- InterPro:   IPR012319
- InterPro:   IPR020629
- InterPro:   IPR010979
- InterPro:   IPR000214
- InterPro:   IPR010663
- SMART:   SM00898
- TIGRFAMs:   TIGR00577

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH; PF06827 zf-FPG_IleRS; SSF81624 Form_DNAglyc_cat; SSF46946 Ribosomal_H2TH

EC number: =3.2.2.23; =4.2.99.18

Molecular weight: Translated: 29931; Mature: 29800

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS51068 FPG_CAT; PS01242 ZF_FPG_1; PS51066 ZF_FPG_2

Important sites: ACT_SITE 2-2 ACT_SITE 3-3 ACT_SITE 58-58 ACT_SITE 260-260 BINDING 91-91 BINDING 109-109 BINDING 151-151

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELPEVETTRRGVEPHLEGRTLLGAVVRNPSLRWPVPPDLSERVAGEKVLAVRRRAKYL
CCCCCCCHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEE
LLECESGTLLIHLGMSGSLRVMPAGAPPQKHDHLDLLLGEQVLRFRDPRRFGAVLWHLGP
EEEECCCEEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCC
VEMHPLLQALGPEPLSDAFDGAALHQAIRRRGSPIKLAIMDNHVVVGVGNIYANESLFHA
HHHHHHHHHHCCCCCHHHHCHHHHHHHHHHCCCCEEEEEECCEEEEEECCCCCCCCHHHC
GISPARAACDLSRADCDRLAAEIKAVLRRAIDAGGSTLRDFVDSEGKPGYFQQTYMVYNR
CCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHEEEEECC
QEEPCRLCGTPIRQIRQGQRSTYYCPLCQP
CCCCHHHCCCHHHHHHCCCCCCEECCCCCC
>Mature Secondary Structure 
PELPEVETTRRGVEPHLEGRTLLGAVVRNPSLRWPVPPDLSERVAGEKVLAVRRRAKYL
CCCCCCHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEE
LLECESGTLLIHLGMSGSLRVMPAGAPPQKHDHLDLLLGEQVLRFRDPRRFGAVLWHLGP
EEEECCCEEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCC
VEMHPLLQALGPEPLSDAFDGAALHQAIRRRGSPIKLAIMDNHVVVGVGNIYANESLFHA
HHHHHHHHHHCCCCCHHHHCHHHHHHHHHHCCCCEEEEEECCEEEEEECCCCCCCCHHHC
GISPARAACDLSRADCDRLAAEIKAVLRRAIDAGGSTLRDFVDSEGKPGYFQQTYMVYNR
CCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHEEEEECC
QEEPCRLCGTPIRQIRQGQRSTYYCPLCQP
CCCCHHHCCCHHHHHHCCCCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782