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The map label for this gene is epsE [H]

Identifier: 34499268

GI number: 34499268

Start: 4110296

End: 4111756

Strand: Reverse

Name: epsE [H]

Synonym: CV_3813

Alternate gene names: 34499268

Gene position: 4111756-4110296 (Counterclockwise)

Preceding gene: 34499269

Following gene: 34499267

Centisome position: 86.54

GC content: 69.34

Gene sequence:

>1461_bases
ATGATGCAAGCCAAGTTCTCGACGCTGCTGCCCTTTCCCTTCGCCCGCGCCAACGGCGTCATCCTGCTGGACGGCGAGGC
GGGGCGGGTGCTGCTGCTGCGCGAGGATGCCAAGCCGGCGGCATGGGGCGAGGCGCTGCGGGTGCATGGCGGCCAGCTGT
CCGGCGTGGAGACGCTGACGGCCGCCCAGTTCGAGAAGCGCCTGTCCGAGCATTACGCCAGCCGCGACGGCGCGGCGGCG
GAAATGGTGGACGACATCGGCCAGGACCTGGATCTGAACCAGATGGTGGAGTCGCTGCCCACCGTCGAGGATCTGCTGGA
GAGCGAGGGCGACGCCCCCATCATCCGCATGATCAACGCGCTGCTGACCCAGGCGCTGCGAGACGGCGCGTCCGACATCC
ACATCGAGGCGTTCGAGGACCGCTCGGTGGTGCGCTTCCGGCTGGACGGCACGCTGCGCGACGTGGTCAGCCCGCATCGC
GCCCTGCACGCGGCGATGGTGTCGCGGATCAAGATCATGGCCAACCTGGACATCGCCGAGAAGCGCATCCCGCAGGACGG
CCGCATCTCGCTGCGCCTGGGCGGCCGGCCGGTGGACGTGCGCGTGTCGACGCTGCCCACCAGCCACGGCGAGCGGGTGG
TGCTGCGCCTGTTGGACAAGGAGAACGCCAAGCTCGACCTGGCCACGCTGGGCATGGCCGCCGACACGCTGGCCTCAGTG
GACAAGCTGATCCGCCAGCCGCACGGCATCCTGCTGGTGACCGGCCCCACCGGCTCCGGCAAGACCACGACCTTGTACGC
CGCCCTGTCGCGGCTGGACGCCAGCCACACCAACATCATGACGGTGGAGGACCCGGTGGAGTACCACCTGGACGGCGTCG
GCCAGACCCAGGTCAACGCCCGCATCGACATGACCTTCGCCAAGGCGCTGCGCGCGATCCTGCGCCAGGACCCGGATGTG
GTGATGATAGGCGAGATCCGCGACCTGGAAACCGCGCAGATCGCGGTGCAGGCGTCCTTGACCGGCCACCTGGTGTTGGC
CACGCTGCACACCAACGACGCCGCCAGCGCCGTCACCCGCCTCACCGACATGGGCGTCGAGCCCTTCCTGCTGGCGTCCA
GCCTGCTGGGCGTGATGGCGCAGCGGCTGGTGCGCCGCGTCTGCCCGCAGTGCAAGGCGCCGCACCCGGTGCCGCTGGAA
GACGCGCCGGAAGTTGTCCATTACCGGCCGGTGGGCTGCCCGGCTTGCGGCCAGTCTGGTTACAAGGGCCGCTACGGCAT
CTACGAATTGATGCTGGTCGACGACACCATGCAGCGGCTGATCCATGATCGCGCGTCCGAGCACAGGCTGCGCGACCACG
CCGACCGCCACGGCATGCGCAGCCTGCGCGACGACGGCATGCGCTGGGTCAAGGCCGGCGAGACCAGCCTGGAAGAAATC
CTGCGGGTGACGAGGAGCTGA

Upstream 100 bases:

>100_bases
TCAACGACCAGTTGAAGACGCCGGCCGCCGGCAGTATCCATGGCGCGTTGAACGCGCTGGATACGTTGAACAAGTCGGAA
GACAACACCAAGGCCAAGCA

Downstream 100 bases:

>100_bases
TGGCGGCGTTCAAGTACACCGCCTACGACGCGGCGGGCAAGGAGCAGAGCGGGCTGCTGGAGGCCGACTCCGCGCGCGCC
GCGCGCGCGCAGCTCAGGGA

Product: type II secretion system protein

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MMQAKFSTLLPFPFARANGVILLDGEAGRVLLLREDAKPAAWGEALRVHGGQLSGVETLTAAQFEKRLSEHYASRDGAAA
EMVDDIGQDLDLNQMVESLPTVEDLLESEGDAPIIRMINALLTQALRDGASDIHIEAFEDRSVVRFRLDGTLRDVVSPHR
ALHAAMVSRIKIMANLDIAEKRIPQDGRISLRLGGRPVDVRVSTLPTSHGERVVLRLLDKENAKLDLATLGMAADTLASV
DKLIRQPHGILLVTGPTGSGKTTTLYAALSRLDASHTNIMTVEDPVEYHLDGVGQTQVNARIDMTFAKALRAILRQDPDV
VMIGEIRDLETAQIAVQASLTGHLVLATLHTNDAASAVTRLTDMGVEPFLLASSLLGVMAQRLVRRVCPQCKAPHPVPLE
DAPEVVHYRPVGCPACGQSGYKGRYGIYELMLVDDTMQRLIHDRASEHRLRDHADRHGMRSLRDDGMRWVKAGETSLEEI
LRVTRS

Sequences:

>Translated_486_residues
MMQAKFSTLLPFPFARANGVILLDGEAGRVLLLREDAKPAAWGEALRVHGGQLSGVETLTAAQFEKRLSEHYASRDGAAA
EMVDDIGQDLDLNQMVESLPTVEDLLESEGDAPIIRMINALLTQALRDGASDIHIEAFEDRSVVRFRLDGTLRDVVSPHR
ALHAAMVSRIKIMANLDIAEKRIPQDGRISLRLGGRPVDVRVSTLPTSHGERVVLRLLDKENAKLDLATLGMAADTLASV
DKLIRQPHGILLVTGPTGSGKTTTLYAALSRLDASHTNIMTVEDPVEYHLDGVGQTQVNARIDMTFAKALRAILRQDPDV
VMIGEIRDLETAQIAVQASLTGHLVLATLHTNDAASAVTRLTDMGVEPFLLASSLLGVMAQRLVRRVCPQCKAPHPVPLE
DAPEVVHYRPVGCPACGQSGYKGRYGIYELMLVDDTMQRLIHDRASEHRLRDHADRHGMRSLRDDGMRWVKAGETSLEEI
LRVTRS
>Mature_486_residues
MMQAKFSTLLPFPFARANGVILLDGEAGRVLLLREDAKPAAWGEALRVHGGQLSGVETLTAAQFEKRLSEHYASRDGAAA
EMVDDIGQDLDLNQMVESLPTVEDLLESEGDAPIIRMINALLTQALRDGASDIHIEAFEDRSVVRFRLDGTLRDVVSPHR
ALHAAMVSRIKIMANLDIAEKRIPQDGRISLRLGGRPVDVRVSTLPTSHGERVVLRLLDKENAKLDLATLGMAADTLASV
DKLIRQPHGILLVTGPTGSGKTTTLYAALSRLDASHTNIMTVEDPVEYHLDGVGQTQVNARIDMTFAKALRAILRQDPDV
VMIGEIRDLETAQIAVQASLTGHLVLATLHTNDAASAVTRLTDMGVEPFLLASSLLGVMAQRLVRRVCPQCKAPHPVPLE
DAPEVVHYRPVGCPACGQSGYKGRYGIYELMLVDDTMQRLIHDRASEHRLRDHADRHGMRSLRDDGMRWVKAGETSLEEI
LRVTRS

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=441, Percent_Identity=55.5555555555556, Blast_Score=493, Evalue=1e-141,
Organism=Escherichia coli, GI1786296, Length=383, Percent_Identity=44.9086161879896, Blast_Score=320, Evalue=9e-89,
Organism=Escherichia coli, GI87082188, Length=195, Percent_Identity=42.5641025641026, Blast_Score=121, Evalue=9e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 53275; Mature: 53275

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQAKFSTLLPFPFARANGVILLDGEAGRVLLLREDAKPAAWGEALRVHGGQLSGVETLT
CCCCCCHHHCCCCCCCCCCEEEEECCCCEEEEEECCCCCCHHHHHHHCCCCCCCCHHHHH
AAQFEKRLSEHYASRDGAAAEMVDDIGQDLDLNQMVESLPTVEDLLESEGDAPIIRMINA
HHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHCCCCCHHHHHHHH
LLTQALRDGASDIHIEAFEDRSVVRFRLDGTLRDVVSPHRALHAAMVSRIKIMANLDIAE
HHHHHHHCCCCCEEEEEECCCCEEEEEECCHHHHHHCHHHHHHHHHHHHHHHHCCCCHHH
KRIPQDGRISLRLGGRPVDVRVSTLPTSHGERVVLRLLDKENAKLDLATLGMAADTLASV
HHCCCCCEEEEEECCEEEEEEEEECCCCCCCEEHHEECCCCCCEEEHHHHHHHHHHHHHH
DKLIRQPHGILLVTGPTGSGKTTTLYAALSRLDASHTNIMTVEDPVEYHLDGVGQTQVNA
HHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHCCCCCCEEECE
RIDMTFAKALRAILRQDPDVVMIGEIRDLETAQIAVQASLTGHLVLATLHTNDAASAVTR
EEHHHHHHHHHHHHHCCCCEEEECCCCCCCHHEEEEEEECCCEEEEEEEECCHHHHHHHH
LTDMGVEPFLLASSLLGVMAQRLVRRVCPQCKAPHPVPLEDAPEVVHYRPVGCPACGQSG
HHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCC
YKGRYGIYELMLVDDTMQRLIHDRASEHRLRDHADRHGMRSLRDDGMRWVKAGETSLEEI
CCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHH
LRVTRS
HHHHCC
>Mature Secondary Structure
MMQAKFSTLLPFPFARANGVILLDGEAGRVLLLREDAKPAAWGEALRVHGGQLSGVETLT
CCCCCCHHHCCCCCCCCCCEEEEECCCCEEEEEECCCCCCHHHHHHHCCCCCCCCHHHHH
AAQFEKRLSEHYASRDGAAAEMVDDIGQDLDLNQMVESLPTVEDLLESEGDAPIIRMINA
HHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHCCCCCHHHHHHHH
LLTQALRDGASDIHIEAFEDRSVVRFRLDGTLRDVVSPHRALHAAMVSRIKIMANLDIAE
HHHHHHHCCCCCEEEEEECCCCEEEEEECCHHHHHHCHHHHHHHHHHHHHHHHCCCCHHH
KRIPQDGRISLRLGGRPVDVRVSTLPTSHGERVVLRLLDKENAKLDLATLGMAADTLASV
HHCCCCCEEEEEECCEEEEEEEEECCCCCCCEEHHEECCCCCCEEEHHHHHHHHHHHHHH
DKLIRQPHGILLVTGPTGSGKTTTLYAALSRLDASHTNIMTVEDPVEYHLDGVGQTQVNA
HHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHCCCCCCEEECE
RIDMTFAKALRAILRQDPDVVMIGEIRDLETAQIAVQASLTGHLVLATLHTNDAASAVTR
EEHHHHHHHHHHHHHCCCCEEEECCCCCCCHHEEEEEEECCCEEEEEEEECCHHHHHHHH
LTDMGVEPFLLASSLLGVMAQRLVRRVCPQCKAPHPVPLEDAPEVVHYRPVGCPACGQSG
HHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCC
YKGRYGIYELMLVDDTMQRLIHDRASEHRLRDHADRHGMRSLRDDGMRWVKAGETSLEEI
CCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHH
LRVTRS
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]