Click here to switch to the map view.

The map label for this gene is ubiB [H]

Identifier: 34499239

GI number: 34499239

Start: 4081125

End: 4082153

Strand: Reverse

Name: ubiB [H]

Synonym: CV_3784

Alternate gene names: 34499239

Gene position: 4082153-4081125 (Counterclockwise)

Preceding gene: 34499242

Following gene: 34499238

Centisome position: 85.92

GC content: 65.01

Gene sequence:

>1029_bases
ATGACTTGCCAGGTGAAGGTGCTCCCCAGCGGGCATACATTCGGTGTCGAAGCGCATGAAACCATTCTTGAAGCCGCGCT
GCGCCAGGGCGTGGGCCTGCCTTACGGCTGCCGCGACGGCGCCTGCGGCGCTTGCAAGGGCAAGGTGCTGGAAGGGGAAG
TGAGCCAGGACGGCTTCCAGGAGAAGGCGCTGTCCGCGGCCGAGCGCGCTCAGGGCATGGCGCTGTTCTGCTGTTCCCGG
CCCAAGGGCGATGTGAGCATCGAGGCCCGGGAAGTGACGGGCGTCGGCGACATCCAGATCAAGACGCTGCCGTGCCGGGT
GGAAAAGATCGACAAGATCCATGACGTGGCGGTGCTGAAGCTGAAGCTGCCGGTGTCCGAGCGCTTGCAGTTCCGCGCCG
GCCAGTACATCGATATCCTGATGAAGGACGGCAAGAAGCGCAGTTTCTCGATCGCCAACGCGCCGCATGACGATGCCTTC
CTCGAGCTGCACATCCGCCATCAGCCCGGCGGCTCCTTCTCCGAGTACGTGTTCCACCAGATGAAGGAACGCGAGATCAT
GCGCTTCAAGGGGCCGATGGGTTCCTTCTTCCTGCGCGAAGAGTCGGACAAGCCCATCGTGCTGATCGCCAGCGGCACCG
GCTTCGCGCCGGTCAAGGGCATCATCGAGCATGCCATCCACCACGGCATCACGCGGCCGATGCAGTTCTACTGGGGCGCC
CGCACCAAGGCCGACCTCTATATGTCGGAACTGGCCGAAGGCTGGGCGGCCGCGCATCCGAACATCCGTTACATCCCGGT
GTTGTCCGAGGCGCTGCCGGAAGACGGCTGGACCGGCCGCACCGGGTTCGTCCACCAGGCGGTGCTGGAAGACTTCGCCG
ATCTGTCCGGCCACCAAGTGTACGCCTGCGGCGCGCCGGTGATGGTGGAGGCGGCGCACGGCACCTTCATCCGCGAACGC
GGCCTGCCCGAGGACGAGTTCTTCTCCGACGCCTTCTTCCTGGCCAAGGACATGGGCGGCGGCAAGTAA

Upstream 100 bases:

>100_bases
GCAGCAGCGGAAGGGCGCGCCGCGCGACATCGCCGGCGCCGATGATGAGCAAAGTACGCATGGTGGCATTTTAGCCAGAA
TTCGGGATTGAGAAACGATT

Downstream 100 bases:

>100_bases
ATACACAAGCTGCTGATCTGTATATGAAAAAGCCCCCGGCAATCGCCGGGGGCTTTTTGTTTGTCCTGCGTCAAAAGGCC
GGGGCGAGCGGTTTGACCTG

Product: CDP-6-deoxy-delta-3,4-glucoseen reductase

Products: NAD(P); FMNH2; 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: CDP-6-deoxy-delta-3,4-glucoseen reductase; E3 [H]

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MTCQVKVLPSGHTFGVEAHETILEAALRQGVGLPYGCRDGACGACKGKVLEGEVSQDGFQEKALSAAERAQGMALFCCSR
PKGDVSIEAREVTGVGDIQIKTLPCRVEKIDKIHDVAVLKLKLPVSERLQFRAGQYIDILMKDGKKRSFSIANAPHDDAF
LELHIRHQPGGSFSEYVFHQMKEREIMRFKGPMGSFFLREESDKPIVLIASGTGFAPVKGIIEHAIHHGITRPMQFYWGA
RTKADLYMSELAEGWAAAHPNIRYIPVLSEALPEDGWTGRTGFVHQAVLEDFADLSGHQVYACGAPVMVEAAHGTFIRER
GLPEDEFFSDAFFLAKDMGGGK

Sequences:

>Translated_342_residues
MTCQVKVLPSGHTFGVEAHETILEAALRQGVGLPYGCRDGACGACKGKVLEGEVSQDGFQEKALSAAERAQGMALFCCSR
PKGDVSIEAREVTGVGDIQIKTLPCRVEKIDKIHDVAVLKLKLPVSERLQFRAGQYIDILMKDGKKRSFSIANAPHDDAF
LELHIRHQPGGSFSEYVFHQMKEREIMRFKGPMGSFFLREESDKPIVLIASGTGFAPVKGIIEHAIHHGITRPMQFYWGA
RTKADLYMSELAEGWAAAHPNIRYIPVLSEALPEDGWTGRTGFVHQAVLEDFADLSGHQVYACGAPVMVEAAHGTFIRER
GLPEDEFFSDAFFLAKDMGGGK
>Mature_341_residues
TCQVKVLPSGHTFGVEAHETILEAALRQGVGLPYGCRDGACGACKGKVLEGEVSQDGFQEKALSAAERAQGMALFCCSRP
KGDVSIEAREVTGVGDIQIKTLPCRVEKIDKIHDVAVLKLKLPVSERLQFRAGQYIDILMKDGKKRSFSIANAPHDDAFL
ELHIRHQPGGSFSEYVFHQMKEREIMRFKGPMGSFFLREESDKPIVLIASGTGFAPVKGIIEHAIHHGITRPMQFYWGAR
TKADLYMSELAEGWAAAHPNIRYIPVLSEALPEDGWTGRTGFVHQAVLEDFADLSGHQVYACGAPVMVEAAHGTFIRERG
LPEDEFFSDAFFLAKDMGGGK

Specific function: Participates in the conversion of CDP-6-deoxy-D-glycero- L-threo-4-hexulose to 3,6-dideoxy-D-glycero-D-glycero-4-hexulose together with CDP-6-deoxy-D-glycero-L-threo-4-hexulose-3-dehydrase (E1) in two consecutive steps. The detailed mechanism of E3 is not

COG id: COG0543

COG function: function code HC; 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367314, Length=234, Percent_Identity=35.042735042735, Blast_Score=149, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6323552, Length=211, Percent_Identity=24.6445497630332, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.5.1.29 [C]

Molecular weight: Translated: 37593; Mature: 37461

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTCQVKVLPSGHTFGVEAHETILEAALRQGVGLPYGCRDGACGACKGKVLEGEVSQDGFQ
CCEEEEEECCCCEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCHHH
EKALSAAERAQGMALFCCSRPKGDVSIEAREVTGVGDIQIKTLPCRVEKIDKIHDVAVLK
HHHHHHHHHHCCEEEEEECCCCCCEEEEEEEECCCCCEEEEEECCHHHHHHHHHCEEEEE
LKLPVSERLQFRAGQYIDILMKDGKKRSFSIANAPHDDAFLELHIRHQPGGSFSEYVFHQ
EECCHHHHHHHCCCCEEEEEECCCCCCEEEECCCCCCCCEEEEEEEECCCCCHHHHHHHH
MKEREIMRFKGPMGSFFLREESDKPIVLIASGTGFAPVKGIIEHAIHHGITRPMQFYWGA
HHHHHHHHHCCCCCCEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHCCC
RTKADLYMSELAEGWAAAHPNIRYIPVLSEALPEDGWTGRTGFVHQAVLEDFADLSGHQV
CCHHHHHHHHHHHHHHHCCCCEEEEEEHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEE
YACGAPVMVEAAHGTFIRERGLPEDEFFSDAFFLAKDMGGGK
EEECCCEEEEECCCCCHHHCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TCQVKVLPSGHTFGVEAHETILEAALRQGVGLPYGCRDGACGACKGKVLEGEVSQDGFQ
CEEEEEECCCCEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCHHH
EKALSAAERAQGMALFCCSRPKGDVSIEAREVTGVGDIQIKTLPCRVEKIDKIHDVAVLK
HHHHHHHHHHCCEEEEEECCCCCCEEEEEEEECCCCCEEEEEECCHHHHHHHHHCEEEEE
LKLPVSERLQFRAGQYIDILMKDGKKRSFSIANAPHDDAFLELHIRHQPGGSFSEYVFHQ
EECCHHHHHHHCCCCEEEEEECCCCCCEEEECCCCCCCCEEEEEEEECCCCCHHHHHHHH
MKEREIMRFKGPMGSFFLREESDKPIVLIASGTGFAPVKGIIEHAIHHGITRPMQFYWGA
HHHHHHHHHCCCCCCEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHCCC
RTKADLYMSELAEGWAAAHPNIRYIPVLSEALPEDGWTGRTGFVHQAVLEDFADLSGHQV
CCHHHHHHHHHHHHHHHCCCCEEEEEEHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEE
YACGAPVMVEAAHGTFIRERGLPEDEFFSDAFFLAKDMGGGK
EEECCCEEEEECCCCCHHHCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD(P)H2; FMN [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H2; FMN; 2 2-octaprenylphenol; O2 [C]

Specific reaction: NAD(P)H2 + FMN = NAD(P) + FMNH2 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10931327; 11586360; 12142430 [H]