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The map label for this gene is spoT [H]

Identifier: 34499223

GI number: 34499223

Start: 4065809

End: 4067965

Strand: Reverse

Name: spoT [H]

Synonym: CV_3768

Alternate gene names: 34499223

Gene position: 4067965-4065809 (Counterclockwise)

Preceding gene: 34499224

Following gene: 34499222

Centisome position: 85.62

GC content: 65.83

Gene sequence:

>2157_bases
ATGGGCACCGGCATTGAGACAGGCATCGACTACAACGTCCTGATCGAGTCCGAAGCGGCGGCATTCCTGGAGAGCGCCGC
GCGCTACCTCAAGCCGGAAGACGTCGAAATGCTCAGGCTGGCCTTCCAGGTCAGCCGCGATGCGCACGAAGGCCAGACCC
GCAAGAGCGGCGAGCCCTACATCACCCATCCGCTGGCGGTGGCGACCATGCTCACCGACTGGCGGCTGGACGTGCAGGGC
CTGGCTGCGGCGCTGCTGCACGACGTGCTGGAAGACACCGGCGTCACCAAGCCGACGCTGGTGGAGAAGTTCGGCAAGAT
CGTCGCCGACCTGGTCGACGGCCTGTCCAAGCTGGAGCGGCTGGAGTATCAGACCAAGGAAGACGCCCAGGCCGAAAGCT
TCCGCAAGATGGTGCTGGCGATGGCGCGCGACATCCGCGTCATCATCGTCAAGCTGGCGGACCGGCTGCACAATATGCGC
ACGCTGGACTCGATGCGCGAGGACAAGCGCCGCCGCATCGCCCTGGAAACGCTGGAGATCTACGCGCCGATCGCCAACCG
CATCGGCCTGAACAAGGTTTACCGCGAGCTGCAGGACCTGGCGTTCAAGCATCTGCACCCGCACCGCTACAGCGTGCTGT
CCAAGGCGGTGCGCGCCGCGCGCGGCAACCGCCGCGAAGTGGTGAACAAGATCCTGCAGGCGGTCAGCCAGCGGCTGGTG
CAAAGCAATATCGAGGCCACGATCAAGGGCCGCGAAAAGAACCTTTACAGCATCTACAAGAAAATGCAGGAGAAGCACCT
GTCCTTCTCCGAGGTGCTGGACATCTACGGCTTCCGCGTCGTCGTCAACGACATTCCGGCCTGCTACCTGGCGCTGGGCG
CGCTGCACAGCCTGTACAAGCCGATTCCCGGCAAATTCAAGGATTACATCGCCATTCCCAAGGGCAATGGCTACCAGAGC
CTGCACACCACGCTGTTCGGCCCCTACGGCACGCCGGTGGAGATGCAGATCCGCACCCGCGAGATGCACAACGTGGCCGA
GGCCGGCGTGGCCTCGCACTGGATGTACAAGAGCGGCGAGGGCGTCAACACCGCGCAGCAGCGCACCCACCAGTGGCTGC
AGAAGCTGCTGGACATGCAGGAGGAGAGCGACGACGCCGTCGAATTCCTCGAGCACATCAAAATCGACCTGTTCCCGGAC
GAGGTTTACGTGTTCACGCCCAAGGGCAAGATCATGGTGCTGCCGCAAGGCTCCACGCCGGTGGATTTCGCCTACGCGGT
GCATACCGACGTCGGCCACCGCTGCATCGCCGCGCGCGTCAACCACGCGCTAGTGCCCTTGCGCACCGCGCTCCGGAACG
GCGACACCGTCGAGATCATCACTTCCACCCAGGCCAAGCCCAATCCGTCGTGGCTGGCCTTCGTGCAGAGCGGCCGCGCC
CGCTCCGGCATCCGCAATTATCTGAAGAGCCTGCAGCGCGAGGAGGCTGTGGCGCTGGGCGAAAAGCTGCTGCGGCAGGC
GGTGGGCGCGCTGGCGTCGACGCCGCTGGCGGTCAGCGACGAACTGCGCGCCGAATACTTCGCCGCCTACGGCGAGAAGA
TGCAGGGCTTCGACGACGTGCTGGCCGACATCGGCGCCGGCAAGCTGCTGCCCATCGTGGTGGCGCGCCGCATGCTGGAG
CTGGCCGGAGAGCGGCTGGGCGAAGGCGTGCGGGTCGGCCCGATCACCATCCGCGGCAACGAGGCCGGCATGGTGCAGCT
GTCCAACTGCTGCAACCCCTTGCCCGGCGACGACATCCTGGGCGTGATCACCAAGGGCCAGGGACTGGTGATCCACCGCG
TCAACTGCCCGAACGCGCGCCGCGCGGATCCGGACAAGCTGCTGGAAGTGACCTGGGAAGCGCAGCGCGACCGGATGTTC
GGCGTCACCGTCGGCGTGCTGTCGCGCAACGAGCGCGGCGCGCTGGCCGATATCGCCACCGCGATTTCGCAGGCGTCGGC
CAATATCGAGAGCGCCGATACCCAGGACAGCAGCCGCGACGGCGACAGCCTGTTCAATATCCACTTCCGCCTGCAGGTGG
AAGGCGTGGAGCACCTGGAGCGCGTGCTCGCCGCCGTCCGCCAACTGGCGGTGGTGCAGCGCGTGGAGAGAAGATGA

Upstream 100 bases:

>100_bases
AGCCGACCGTGATCGCCCTGCGTGAAATTGCCGCCGGCCACGTCGGCAAGGAAGTCCTCAACCGCGGCAAGGCCTGATTG
ACGCAGTACGGAGCGCGAAG

Downstream 100 bases:

>100_bases
GTCAATTGAATCTGCGCATCAACGGCGAAGATCGGAGTTTCTCGGGCATCGCCACCTTCGCCGAGCTGGTCGAAGCCTTG
CAGCTGGGCGGCAAGCGCAT

Product: guanosine-3',5'-bis(diphosphate) 3'-diphosphatase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 718; Mature: 717

Protein sequence:

>718_residues
MGTGIETGIDYNVLIESEAAAFLESAARYLKPEDVEMLRLAFQVSRDAHEGQTRKSGEPYITHPLAVATMLTDWRLDVQG
LAAALLHDVLEDTGVTKPTLVEKFGKIVADLVDGLSKLERLEYQTKEDAQAESFRKMVLAMARDIRVIIVKLADRLHNMR
TLDSMREDKRRRIALETLEIYAPIANRIGLNKVYRELQDLAFKHLHPHRYSVLSKAVRAARGNRREVVNKILQAVSQRLV
QSNIEATIKGREKNLYSIYKKMQEKHLSFSEVLDIYGFRVVVNDIPACYLALGALHSLYKPIPGKFKDYIAIPKGNGYQS
LHTTLFGPYGTPVEMQIRTREMHNVAEAGVASHWMYKSGEGVNTAQQRTHQWLQKLLDMQEESDDAVEFLEHIKIDLFPD
EVYVFTPKGKIMVLPQGSTPVDFAYAVHTDVGHRCIAARVNHALVPLRTALRNGDTVEIITSTQAKPNPSWLAFVQSGRA
RSGIRNYLKSLQREEAVALGEKLLRQAVGALASTPLAVSDELRAEYFAAYGEKMQGFDDVLADIGAGKLLPIVVARRMLE
LAGERLGEGVRVGPITIRGNEAGMVQLSNCCNPLPGDDILGVITKGQGLVIHRVNCPNARRADPDKLLEVTWEAQRDRMF
GVTVGVLSRNERGALADIATAISQASANIESADTQDSSRDGDSLFNIHFRLQVEGVEHLERVLAAVRQLAVVQRVERR

Sequences:

>Translated_718_residues
MGTGIETGIDYNVLIESEAAAFLESAARYLKPEDVEMLRLAFQVSRDAHEGQTRKSGEPYITHPLAVATMLTDWRLDVQG
LAAALLHDVLEDTGVTKPTLVEKFGKIVADLVDGLSKLERLEYQTKEDAQAESFRKMVLAMARDIRVIIVKLADRLHNMR
TLDSMREDKRRRIALETLEIYAPIANRIGLNKVYRELQDLAFKHLHPHRYSVLSKAVRAARGNRREVVNKILQAVSQRLV
QSNIEATIKGREKNLYSIYKKMQEKHLSFSEVLDIYGFRVVVNDIPACYLALGALHSLYKPIPGKFKDYIAIPKGNGYQS
LHTTLFGPYGTPVEMQIRTREMHNVAEAGVASHWMYKSGEGVNTAQQRTHQWLQKLLDMQEESDDAVEFLEHIKIDLFPD
EVYVFTPKGKIMVLPQGSTPVDFAYAVHTDVGHRCIAARVNHALVPLRTALRNGDTVEIITSTQAKPNPSWLAFVQSGRA
RSGIRNYLKSLQREEAVALGEKLLRQAVGALASTPLAVSDELRAEYFAAYGEKMQGFDDVLADIGAGKLLPIVVARRMLE
LAGERLGEGVRVGPITIRGNEAGMVQLSNCCNPLPGDDILGVITKGQGLVIHRVNCPNARRADPDKLLEVTWEAQRDRMF
GVTVGVLSRNERGALADIATAISQASANIESADTQDSSRDGDSLFNIHFRLQVEGVEHLERVLAAVRQLAVVQRVERR
>Mature_717_residues
GTGIETGIDYNVLIESEAAAFLESAARYLKPEDVEMLRLAFQVSRDAHEGQTRKSGEPYITHPLAVATMLTDWRLDVQGL
AAALLHDVLEDTGVTKPTLVEKFGKIVADLVDGLSKLERLEYQTKEDAQAESFRKMVLAMARDIRVIIVKLADRLHNMRT
LDSMREDKRRRIALETLEIYAPIANRIGLNKVYRELQDLAFKHLHPHRYSVLSKAVRAARGNRREVVNKILQAVSQRLVQ
SNIEATIKGREKNLYSIYKKMQEKHLSFSEVLDIYGFRVVVNDIPACYLALGALHSLYKPIPGKFKDYIAIPKGNGYQSL
HTTLFGPYGTPVEMQIRTREMHNVAEAGVASHWMYKSGEGVNTAQQRTHQWLQKLLDMQEESDDAVEFLEHIKIDLFPDE
VYVFTPKGKIMVLPQGSTPVDFAYAVHTDVGHRCIAARVNHALVPLRTALRNGDTVEIITSTQAKPNPSWLAFVQSGRAR
SGIRNYLKSLQREEAVALGEKLLRQAVGALASTPLAVSDELRAEYFAAYGEKMQGFDDVLADIGAGKLLPIVVARRMLEL
AGERLGEGVRVGPITIRGNEAGMVQLSNCCNPLPGDDILGVITKGQGLVIHRVNCPNARRADPDKLLEVTWEAQRDRMFG
VTVGVLSRNERGALADIATAISQASANIESADTQDSSRDGDSLFNIHFRLQVEGVEHLERVLAAVRQLAVVQRVERR

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=692, Percent_Identity=48.121387283237, Blast_Score=681, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=676, Percent_Identity=32.396449704142, Blast_Score=400, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 80045; Mature: 79914

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTGIETGIDYNVLIESEAAAFLESAARYLKPEDVEMLRLAFQVSRDAHEGQTRKSGEPY
CCCCCCCCCCEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHCCCCCCCCCCC
ITHPLAVATMLTDWRLDVQGLAAALLHDVLEDTGVTKPTLVEKFGKIVADLVDGLSKLER
EEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LEYQTKEDAQAESFRKMVLAMARDIRVIIVKLADRLHNMRTLDSMREDKRRRIALETLEI
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YAPIANRIGLNKVYRELQDLAFKHLHPHRYSVLSKAVRAARGNRREVVNKILQAVSQRLV
HHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
QSNIEATIKGREKNLYSIYKKMQEKHLSFSEVLDIYGFRVVVNDIPACYLALGALHSLYK
HCCCCEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHC
PIPGKFKDYIAIPKGNGYQSLHTTLFGPYGTPVEMQIRTREMHNVAEAGVASHWMYKSGE
CCCCCCCCEEECCCCCCCHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCC
GVNTAQQRTHQWLQKLLDMQEESDDAVEFLEHIKIDLFPDEVYVFTPKGKIMVLPQGSTP
CCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCEEEEECCCEEEECCCCEEEEEECCCCC
VDFAYAVHTDVGHRCIAARVNHALVPLRTALRNGDTVEIITSTQAKPNPSWLAFVQSGRA
CEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHCCCH
RSGIRNYLKSLQREEAVALGEKLLRQAVGALASTPLAVSDELRAEYFAAYGEKMQGFDDV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHH
LADIGAGKLLPIVVARRMLELAGERLGEGVRVGPITIRGNEAGMVQLSNCCNPLPGDDIL
HHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEHHCCCCCCCCCCE
GVITKGQGLVIHRVNCPNARRADPDKLLEVTWEAQRDRMFGVTVGVLSRNERGALADIAT
EEEECCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHH
AISQASANIESADTQDSSRDGDSLFNIHFRLQVEGVEHLERVLAAVRQLAVVQRVERR
HHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GTGIETGIDYNVLIESEAAAFLESAARYLKPEDVEMLRLAFQVSRDAHEGQTRKSGEPY
CCCCCCCCCEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHCCCCCCCCCCC
ITHPLAVATMLTDWRLDVQGLAAALLHDVLEDTGVTKPTLVEKFGKIVADLVDGLSKLER
EEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LEYQTKEDAQAESFRKMVLAMARDIRVIIVKLADRLHNMRTLDSMREDKRRRIALETLEI
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YAPIANRIGLNKVYRELQDLAFKHLHPHRYSVLSKAVRAARGNRREVVNKILQAVSQRLV
HHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
QSNIEATIKGREKNLYSIYKKMQEKHLSFSEVLDIYGFRVVVNDIPACYLALGALHSLYK
HCCCCEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHC
PIPGKFKDYIAIPKGNGYQSLHTTLFGPYGTPVEMQIRTREMHNVAEAGVASHWMYKSGE
CCCCCCCCEEECCCCCCCHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCC
GVNTAQQRTHQWLQKLLDMQEESDDAVEFLEHIKIDLFPDEVYVFTPKGKIMVLPQGSTP
CCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCEEEEECCCEEEECCCCEEEEEECCCCC
VDFAYAVHTDVGHRCIAARVNHALVPLRTALRNGDTVEIITSTQAKPNPSWLAFVQSGRA
CEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHCCCH
RSGIRNYLKSLQREEAVALGEKLLRQAVGALASTPLAVSDELRAEYFAAYGEKMQGFDDV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHH
LADIGAGKLLPIVVARRMLELAGERLGEGVRVGPITIRGNEAGMVQLSNCCNPLPGDDIL
HHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEHHCCCCCCCCCCE
GVITKGQGLVIHRVNCPNARRADPDKLLEVTWEAQRDRMFGVTVGVLSRNERGALADIAT
EEEECCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHH
AISQASANIESADTQDSSRDGDSLFNIHFRLQVEGVEHLERVLAAVRQLAVVQRVERR
HHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]