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The map label for this gene is aspS

Identifier: 34499195

GI number: 34499195

Start: 4038696

End: 4040489

Strand: Reverse

Name: aspS

Synonym: CV_3740

Alternate gene names: 34499195

Gene position: 4040489-4038696 (Counterclockwise)

Preceding gene: 34499196

Following gene: 34499194

Centisome position: 85.04

GC content: 63.88

Gene sequence:

>1794_bases
ATGCGCACCGATTATTGCGGACTCATCGACAAGAAATACCTCGGTCAAACCGTGACCGTCAAAGGCTGGGCCCATCGCCG
CCGCGACCATGGCGGCGTGATCTTCATCGACCTGCGCGACCGCGAAGGCCTGGTGCAGGTGGTGATCGATCCGGACACCC
CGGAAGCGTTCAAGCTGGCCGACAGCAGCCGCGGCGAGTACGTGCTGTCCATCACCGGCATCGTGCGCGAGCGCCCGGCC
GGCACCGCCAACAGCAAGATGATCTCCGGCGAGATCGAGATCCTGGCCAAGGAAATCGAAATCCTGAACGCCGCGGCCAC
GCCGCCGTTCCAGATCGACGACGAGAACCTGTCCGAGAACGTCCGTCTGACCAACCGCGTGATCGATCTGCGCCGCCCGG
CGATGCAGAAGAACCTGCGCCTGCGCTACAAGGTGGCGATGGGCGTGCGCAACCACCTGGACAAGCAAGGCTTCATCGAC
ATCGAAACCCCGATGCTGACCCGCTCCACCCCGGAAGGCGCCCGCGACTACCTGGTGCCGTCCCGCGTGCATCCGGGCGA
GTTCTTCGCGCTGCCGCAATCGCCGCAGCTGTTCAAGCAGCTGCTGATGGTGGCCGGCTTCGACCGCTACTACCAGATCA
CCAAGTGCTTCCGCGACGAAGACCTGCGCGCCGACCGCCAGCCTGAATTCACCCAGATCGATATCGAGACCTCGTTCCTG
AACGAGGACGAGATCATGGACATCACCGAAGGCATGACCAAGGAGATCTTCCAGGACGTGCTGGGCGTGACGCTGCCGAC
CTTCCCGCGCATGACCTACGGCGACGCGATGTTCTACTACGGCTCCGACAAGCCGGATATGCGCGTTGCGCTGAAATTCA
CCGAACTGACCGACGTGATGAAGTCGGAAGAGTTCAAGGTGTTCCGCGGCGCCGCCGACATGGCGAACGGCCGCGTAGTG
GCCCTGCGCGTGCCGAACGGCGCGTCGTTCAGCCGCAAGGAAATCGACGACTACACCCAGTTCGTCGCCATCTACGGCGC
CAAGGGCCTGGCCTACATCAAGGTCAACGACGTCACCAAGCTGAACGAAGAGGGCCTGCAGTCGCCCATCGTGAAGTTCC
TGTCCGCCAATGGCCTGAAGGAAATCATAGCCCGCACCGGCGCGCAGAACGGCGACATCATCTTCTTCGGCGCCGACAAG
GCCAAGGTGGTGAACGAGGCGATCGGCGCGCTGCGCATCAAGATCGGCCACGAGCACGGTCTGGAAAACGGCTACTTCGT
CAAGGAATGGCGCCCGCTGTGGGTGGTGGACTTCCCGATGTTCGAGCACGACGAGGAAGAAGACCGCTGGACCGCCTGCC
ACCATCCGTTCACCAGCCCGAAGCCGGGCCACGAGGACCTGATGGCCACCGATCCGGGCAAGTGTCTGGCGCGCGCCTAC
GACATGGTGCTGAACGGCTGGGAAATCGGCGGCGGCTCCATCCGTATCCACCGCGCCGACATCCAGGAGAAGGTGTTCGG
CGCGCTGAAGATCAGCCCGGAAGAGCAGCAGAACAAGTTCGGCTTCCTGCTGGACAACCTGAAGTTCGGCGCGCCGCCGC
ACGGCGGCCTGGCCTTCGGCCTGGATCGCCTGGTGACGCTGATGTGCGGCGCGGAATCGATCCGCGACGTGATCGCCTTC
CCGAAGACCCAACGCGCCCAATGCCTGTTGACCAACGCGCCGAACGCGGTGGACGACAAGCAGCTGCGCGAGCTGAACCT
GCGCCTGCGCCAGAAGGCCGAGCCGAGCGCTTGA

Upstream 100 bases:

>100_bases
TGAACATAATCGGCAGGGCTAGGCCGTCACCCTGTCTAGCCCCTAAGCATGTCGTCCCCTAGCGGGAACTTCGAAGAATT
CAATCAAAAAGGTTTACCCA

Downstream 100 bases:

>100_bases
TTGGGCGGGATCGCCTGGAAAAACGGACAGCTTGCTGTCCGTTTTTTTTTCGCGCCCGGCCGGCTGATATCCGTCGGCTT
GAAACTTAGGCCGGCGATCC

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 597; Mature: 597

Protein sequence:

>597_residues
MRTDYCGLIDKKYLGQTVTVKGWAHRRRDHGGVIFIDLRDREGLVQVVIDPDTPEAFKLADSSRGEYVLSITGIVRERPA
GTANSKMISGEIEILAKEIEILNAAATPPFQIDDENLSENVRLTNRVIDLRRPAMQKNLRLRYKVAMGVRNHLDKQGFID
IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFRDEDLRADRQPEFTQIDIETSFL
NEDEIMDITEGMTKEIFQDVLGVTLPTFPRMTYGDAMFYYGSDKPDMRVALKFTELTDVMKSEEFKVFRGAADMANGRVV
ALRVPNGASFSRKEIDDYTQFVAIYGAKGLAYIKVNDVTKLNEEGLQSPIVKFLSANGLKEIIARTGAQNGDIIFFGADK
AKVVNEAIGALRIKIGHEHGLENGYFVKEWRPLWVVDFPMFEHDEEEDRWTACHHPFTSPKPGHEDLMATDPGKCLARAY
DMVLNGWEIGGGSIRIHRADIQEKVFGALKISPEEQQNKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMCGAESIRDVIAF
PKTQRAQCLLTNAPNAVDDKQLRELNLRLRQKAEPSA

Sequences:

>Translated_597_residues
MRTDYCGLIDKKYLGQTVTVKGWAHRRRDHGGVIFIDLRDREGLVQVVIDPDTPEAFKLADSSRGEYVLSITGIVRERPA
GTANSKMISGEIEILAKEIEILNAAATPPFQIDDENLSENVRLTNRVIDLRRPAMQKNLRLRYKVAMGVRNHLDKQGFID
IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFRDEDLRADRQPEFTQIDIETSFL
NEDEIMDITEGMTKEIFQDVLGVTLPTFPRMTYGDAMFYYGSDKPDMRVALKFTELTDVMKSEEFKVFRGAADMANGRVV
ALRVPNGASFSRKEIDDYTQFVAIYGAKGLAYIKVNDVTKLNEEGLQSPIVKFLSANGLKEIIARTGAQNGDIIFFGADK
AKVVNEAIGALRIKIGHEHGLENGYFVKEWRPLWVVDFPMFEHDEEEDRWTACHHPFTSPKPGHEDLMATDPGKCLARAY
DMVLNGWEIGGGSIRIHRADIQEKVFGALKISPEEQQNKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMCGAESIRDVIAF
PKTQRAQCLLTNAPNAVDDKQLRELNLRLRQKAEPSA
>Mature_597_residues
MRTDYCGLIDKKYLGQTVTVKGWAHRRRDHGGVIFIDLRDREGLVQVVIDPDTPEAFKLADSSRGEYVLSITGIVRERPA
GTANSKMISGEIEILAKEIEILNAAATPPFQIDDENLSENVRLTNRVIDLRRPAMQKNLRLRYKVAMGVRNHLDKQGFID
IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFRDEDLRADRQPEFTQIDIETSFL
NEDEIMDITEGMTKEIFQDVLGVTLPTFPRMTYGDAMFYYGSDKPDMRVALKFTELTDVMKSEEFKVFRGAADMANGRVV
ALRVPNGASFSRKEIDDYTQFVAIYGAKGLAYIKVNDVTKLNEEGLQSPIVKFLSANGLKEIIARTGAQNGDIIFFGADK
AKVVNEAIGALRIKIGHEHGLENGYFVKEWRPLWVVDFPMFEHDEEEDRWTACHHPFTSPKPGHEDLMATDPGKCLARAY
DMVLNGWEIGGGSIRIHRADIQEKVFGALKISPEEQQNKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMCGAESIRDVIAF
PKTQRAQCLLTNAPNAVDDKQLRELNLRLRQKAEPSA

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=607, Percent_Identity=40.8566721581549, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI45439306, Length=265, Percent_Identity=28.3018867924528, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI4758762, Length=290, Percent_Identity=26.551724137931, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI5031815, Length=261, Percent_Identity=26.4367816091954, Blast_Score=80, Evalue=6e-15,
Organism=Homo sapiens, GI194272210, Length=261, Percent_Identity=26.4367816091954, Blast_Score=80, Evalue=6e-15,
Organism=Escherichia coli, GI1788173, Length=594, Percent_Identity=56.0606060606061, Blast_Score=684, Evalue=0.0,
Organism=Escherichia coli, GI1790571, Length=306, Percent_Identity=26.1437908496732, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789256, Length=312, Percent_Identity=26.9230769230769, Blast_Score=92, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI32566633, Length=621, Percent_Identity=35.1046698872786, Blast_Score=315, Evalue=4e-86,
Organism=Caenorhabditis elegans, GI17551876, Length=268, Percent_Identity=27.6119402985075, Blast_Score=94, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71984122, Length=333, Percent_Identity=24.9249249249249, Blast_Score=84, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71994340, Length=267, Percent_Identity=22.8464419475655, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17535925, Length=267, Percent_Identity=22.8464419475655, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17535927, Length=267, Percent_Identity=22.8464419475655, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6325153, Length=632, Percent_Identity=32.4367088607595, Blast_Score=295, Evalue=1e-80,
Organism=Saccharomyces cerevisiae, GI6321807, Length=252, Percent_Identity=26.984126984127, Blast_Score=81, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6323011, Length=287, Percent_Identity=27.5261324041812, Blast_Score=80, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6320242, Length=262, Percent_Identity=24.0458015267176, Blast_Score=71, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6324256, Length=169, Percent_Identity=26.0355029585799, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24584738, Length=613, Percent_Identity=37.0309951060359, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI17136276, Length=244, Percent_Identity=31.9672131147541, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24640849, Length=248, Percent_Identity=27.4193548387097, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24640851, Length=251, Percent_Identity=27.8884462151394, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19921528, Length=229, Percent_Identity=29.2576419213974, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_CHRVO (Q7NRP0)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903410.1
- ProteinModelPortal:   Q7NRP0
- SMR:   Q7NRP0
- GeneID:   2547033
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_3740
- NMPDR:   fig|243365.1.peg.3740
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- PhylomeDB:   Q7NRP0
- ProtClustDB:   PRK00476
- BioCyc:   CVIO243365:CV_3740-MONOMER
- BRENDA:   6.1.1.12
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 67299; Mature: 67299

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTDYCGLIDKKYLGQTVTVKGWAHRRRDHGGVIFIDLRDREGLVQVVIDPDTPEAFKLA
CCCCCCCCCCHHHCCCEEEEECHHHHHCCCCCEEEEEEECCCCEEEEEECCCCCHHEEEC
DSSRGEYVLSITGIVRERPAGTANSKMISGEIEILAKEIEILNAAATPPFQIDDENLSEN
CCCCCCEEEEEEEEHHCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCCCCEECCCCCCCC
VRLTNRVIDLRRPAMQKNLRLRYKVAMGVRNHLDKQGFIDIETPMLTRSTPEGARDYLVP
HHHHHHHHHHHCHHHHCCCEEEEEEEHHHHHCCCCCCEEEECCCEEECCCCCCCHHCCCC
SRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFRDEDLRADRQPEFTQIDIETSFL
CCCCCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEC
NEDEIMDITEGMTKEIFQDVLGVTLPTFPRMTYGDAMFYYGSDKPDMRVALKFTELTDVM
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEEHHHHHHH
KSEEFKVFRGAADMANGRVVALRVPNGASFSRKEIDDYTQFVAIYGAKGLAYIKVNDVTK
HCCCCEEEECCHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHEECCCCEEEEEECCCHH
LNEEGLQSPIVKFLSANGLKEIIARTGAQNGDIIFFGADKAKVVNEAIGALRIKIGHEHG
CCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCEEEECCCHHHHHHHHHCEEEEEECCCCC
LENGYFVKEWRPLWVVDFPMFEHDEEEDRWTACHHPFTSPKPGHEDLMATDPGKCLARAY
CCCCEEEEECCEEEEEECCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHH
DMVLNGWEIGGGSIRIHRADIQEKVFGALKISPEEQQNKFGFLLDNLKFGAPPHGGLAFG
HHHHCCEEECCCEEEEECCCHHHHHHEEEEECCHHHCCCCCEEEECCCCCCCCCCCHHHH
LDRLVTLMCGAESIRDVIAFPKTQRAQCLLTNAPNAVDDKQLRELNLRLRQKAEPSA
HHHHHHHHHCHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRTDYCGLIDKKYLGQTVTVKGWAHRRRDHGGVIFIDLRDREGLVQVVIDPDTPEAFKLA
CCCCCCCCCCHHHCCCEEEEECHHHHHCCCCCEEEEEEECCCCEEEEEECCCCCHHEEEC
DSSRGEYVLSITGIVRERPAGTANSKMISGEIEILAKEIEILNAAATPPFQIDDENLSEN
CCCCCCEEEEEEEEHHCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCCCCEECCCCCCCC
VRLTNRVIDLRRPAMQKNLRLRYKVAMGVRNHLDKQGFIDIETPMLTRSTPEGARDYLVP
HHHHHHHHHHHCHHHHCCCEEEEEEEHHHHHCCCCCCEEEECCCEEECCCCCCCHHCCCC
SRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFRDEDLRADRQPEFTQIDIETSFL
CCCCCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEC
NEDEIMDITEGMTKEIFQDVLGVTLPTFPRMTYGDAMFYYGSDKPDMRVALKFTELTDVM
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEEHHHHHHH
KSEEFKVFRGAADMANGRVVALRVPNGASFSRKEIDDYTQFVAIYGAKGLAYIKVNDVTK
HCCCCEEEECCHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHEECCCCEEEEEECCCHH
LNEEGLQSPIVKFLSANGLKEIIARTGAQNGDIIFFGADKAKVVNEAIGALRIKIGHEHG
CCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCEEEECCCHHHHHHHHHCEEEEEECCCCC
LENGYFVKEWRPLWVVDFPMFEHDEEEDRWTACHHPFTSPKPGHEDLMATDPGKCLARAY
CCCCEEEEECCEEEEEECCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHH
DMVLNGWEIGGGSIRIHRADIQEKVFGALKISPEEQQNKFGFLLDNLKFGAPPHGGLAFG
HHHHCCEEECCCEEEEECCCHHHHHHEEEEECCHHHCCCCCEEEECCCCCCCCCCCHHHH
LDRLVTLMCGAESIRDVIAFPKTQRAQCLLTNAPNAVDDKQLRELNLRLRQKAEPSA
HHHHHHHHHCHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782