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The map label for this gene is cspE [H]

Identifier: 34499122

GI number: 34499122

Start: 3969055

End: 3969258

Strand: Reverse

Name: cspE [H]

Synonym: CV_3667

Alternate gene names: 34499122

Gene position: 3969258-3969055 (Counterclockwise)

Preceding gene: 34499125

Following gene: 34499120

Centisome position: 83.54

GC content: 52.94

Gene sequence:

>204_bases
ATGGCATTTGGTACCGTGAAGTGGTTCAATGACGCGAAAGGCTTCGGCTTTATTACCCCGGACGAAGGTGGCGAGGATCT
GTTCGCCCATTTCTCGGCCATCAACATGCAAGGCTTCAAGACCCTGAAAGAGGGTCAGCGCGTAAGCTTTGAAGTGGTGT
CCGGCCCGAAGGGTAAGCAAGCGTCCAATATTCAGAACGCGTAA

Upstream 100 bases:

>100_bases
TTGGCTTAACTGTTTGGCATTTTGCGGCACAGCATTTTGTCAAACTCTATTAAGCTCTAAGTTGGTCGGCTTCACCAGCC
TTTTTTTGCAAGGAAGTTAA

Downstream 100 bases:

>100_bases
ATACTGTTCTGAATCAGTCTCGCCGTGTGGCGGGCTGCAAGGCATATTTCAAAGCCCGCCTCCGGCGGGCTTTTCATTTG
CGTCGGCGTTTCCCGCTGTC

Product: cold shock transcription regulator protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 67; Mature: 66

Protein sequence:

>67_residues
MAFGTVKWFNDAKGFGFITPDEGGEDLFAHFSAINMQGFKTLKEGQRVSFEVVSGPKGKQASNIQNA

Sequences:

>Translated_67_residues
MAFGTVKWFNDAKGFGFITPDEGGEDLFAHFSAINMQGFKTLKEGQRVSFEVVSGPKGKQASNIQNA
>Mature_66_residues
AFGTVKWFNDAKGFGFITPDEGGEDLFAHFSAINMQGFKTLKEGQRVSFEVVSGPKGKQASNIQNA

Specific function: Inhibits DNA Replication At Both Initiation And Elongation Steps, Most Probably By Binding To The Opened, Single- Stranded Regions At Replication Forks. Plays A Regulatory Role In Chromosomal Replication In Nutrient-Depleted Cells. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1787107, Length=64, Percent_Identity=65.625, Blast_Score=91, Evalue=1e-20,
Organism=Escherichia coli, GI1786841, Length=61, Percent_Identity=63.9344262295082, Blast_Score=89, Evalue=6e-20,
Organism=Escherichia coli, GI1789979, Length=63, Percent_Identity=60.3174603174603, Blast_Score=83, Evalue=4e-18,
Organism=Escherichia coli, GI1788126, Length=61, Percent_Identity=62.2950819672131, Blast_Score=80, Evalue=2e-17,
Organism=Escherichia coli, GI2367114, Length=61, Percent_Identity=54.0983606557377, Blast_Score=75, Evalue=7e-16,
Organism=Escherichia coli, GI1787839, Length=61, Percent_Identity=55.7377049180328, Blast_Score=75, Evalue=8e-16,
Organism=Escherichia coli, GI1787834, Length=61, Percent_Identity=57.3770491803279, Blast_Score=75, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI71983217, Length=61, Percent_Identity=54.0983606557377, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71983225, Length=61, Percent_Identity=54.0983606557377, Blast_Score=72, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24658520, Length=64, Percent_Identity=43.75, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7285; Mature: 7154

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFGTVKWFNDAKGFGFITPDEGGEDLFAHFSAINMQGFKTLKEGQRVSFEVVSGPKGKQ
CCCCCCEECCCCCCCEEECCCCCCHHHHHHHHHCCCCCHHHCCCCCEEEEEEECCCCCCC
ASNIQNA
CCCCCCC
>Mature Secondary Structure 
AFGTVKWFNDAKGFGFITPDEGGEDLFAHFSAINMQGFKTLKEGQRVSFEVVSGPKGKQ
CCCCCEECCCCCCCEEECCCCCCHHHHHHHHHCCCCCHHHCCCCCEEEEEEECCCCCCC
ASNIQNA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA