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The map label for this gene is rluE [H]

Identifier: 34499118

GI number: 34499118

Start: 3964138

End: 3964884

Strand: Reverse

Name: rluE [H]

Synonym: CV_3663

Alternate gene names: 34499118

Gene position: 3964884-3964138 (Counterclockwise)

Preceding gene: 34499120

Following gene: 34499115

Centisome position: 83.45

GC content: 65.46

Gene sequence:

>747_bases
ATGATATCGATCACGAACCGGCCCGGGCTTGCTATCATTCGCCTACCTTATGTTTTCCGTGTCGTCCGCATGCCCCAGCT
CATTCTGCTCAACAAGCCTTACGGCGTGATCTGCCAGTTTTCCGAACACCCGACCCATCCGACGCTGAAGTCCTGCGTGC
CGCAGGCCGGCGTGTATCCGGCCGGACGGCTCGATACCGACAGCGAAGGCCTGCTGCTGCTGACCGGAGACGGCGCCTTG
CAGCACCGCATCGCCGATCCGCGCTGGAAACTGCCCAAGACCTACTGGGTGCAGGTGGAGGGCGACCCGAAAGAAGAACA
GCTGGAGATGTTGCGCAGGGGCGTGGACCTGGGCGATTTCGTCACCCGGCCGGCCGAGGTCCGGCGCATCGAGACGCCGG
AATTATGGCCGCGCAATCCGCCGGTGCGTTTTCGCAAGACGGTGCCGGATTGCTGGCTGGAAATCATCATCAGCGAAGGC
AAGAACCGACAGGTGCGGCGGATGACGGCCAAGGCCGGATTGCCTACGCTGCGCCTGGTGCGGGTGGCCATCGGGCCTTG
GAAACTGGACGGTTTGCAGCCGGGCGAGATGCGCGCGATTGACGTGCGGACTGAGGATTTGCCCAGGGGGGCGAGTGCCG
GCCCGGGCGGCAAGCCAGGCGACCGGGCGCCGCAGAGCCGGAGCGCCGCGGCGGCCAAGAGCGGCAAGATCCCGAGCTTC
CGCTTCGCGCGAAACCGGAAAGGCTAG

Upstream 100 bases:

>100_bases
GTTGGCGGCGCAACTGAAACTTTTATGCCTGATAACTGGCGGCAGGACGTTCGAATGCGAATAAAACGGTTGTTCGAAGG
CCGGCGCCGCGGCGGCGCGC

Downstream 100 bases:

>100_bases
CGCCGTACGGCTGCGGGACGCTTCGTCAGCAAGCGGCCCGCTACGTTCCGGAGGAGCCGAAATGCGAAGCGGGGAAGCCT
GGCTTCCCCGCTCGGGCGCA

Product: pseudouridine synthase

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MISITNRPGLAIIRLPYVFRVVRMPQLILLNKPYGVICQFSEHPTHPTLKSCVPQAGVYPAGRLDTDSEGLLLLTGDGAL
QHRIADPRWKLPKTYWVQVEGDPKEEQLEMLRRGVDLGDFVTRPAEVRRIETPELWPRNPPVRFRKTVPDCWLEIIISEG
KNRQVRRMTAKAGLPTLRLVRVAIGPWKLDGLQPGEMRAIDVRTEDLPRGASAGPGGKPGDRAPQSRSAAAAKSGKIPSF
RFARNRKG

Sequences:

>Translated_248_residues
MISITNRPGLAIIRLPYVFRVVRMPQLILLNKPYGVICQFSEHPTHPTLKSCVPQAGVYPAGRLDTDSEGLLLLTGDGAL
QHRIADPRWKLPKTYWVQVEGDPKEEQLEMLRRGVDLGDFVTRPAEVRRIETPELWPRNPPVRFRKTVPDCWLEIIISEG
KNRQVRRMTAKAGLPTLRLVRVAIGPWKLDGLQPGEMRAIDVRTEDLPRGASAGPGGKPGDRAPQSRSAAAAKSGKIPSF
RFARNRKG
>Mature_248_residues
MISITNRPGLAIIRLPYVFRVVRMPQLILLNKPYGVICQFSEHPTHPTLKSCVPQAGVYPAGRLDTDSEGLLLLTGDGAL
QHRIADPRWKLPKTYWVQVEGDPKEEQLEMLRRGVDLGDFVTRPAEVRRIETPELWPRNPPVRFRKTVPDCWLEIIISEG
KNRQVRRMTAKAGLPTLRLVRVAIGPWKLDGLQPGEMRAIDVRTEDLPRGASAGPGGKPGDRAPQSRSAAAAKSGKIPSF
RFARNRKG

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=176, Percent_Identity=53.9772727272727, Blast_Score=188, Evalue=3e-49,
Organism=Escherichia coli, GI1788510, Length=180, Percent_Identity=36.1111111111111, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1787524, Length=166, Percent_Identity=30.7228915662651, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI1790453, Length=174, Percent_Identity=28.735632183908, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 27717; Mature: 27717

Theoretical pI: Translated: 10.96; Mature: 10.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISITNRPGLAIIRLPYVFRVVRMPQLILLNKPYGVICQFSEHPTHPTLKSCVPQAGVYP
CEEECCCCCEEEEEHHHHHHHHHCCEEEEEECCCCEEEECCCCCCCCCHHHHCCCCCCCC
AGRLDTDSEGLLLLTGDGALQHRIADPRWKLPKTYWVQVEGDPKEEQLEMLRRGVDLGDF
CCCCCCCCCCEEEEECCCHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCHHHH
VTRPAEVRRIETPELWPRNPPVRFRKTVPDCWLEIIISEGKNRQVRRMTAKAGLPTLRLV
HCCCHHHEECCCCCCCCCCCCCHHHHCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEE
RVAIGPWKLDGLQPGEMRAIDVRTEDLPRGASAGPGGKPGDRAPQSRSAAAAKSGKIPSF
EEEECCEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCE
RFARNRKG
ECCCCCCC
>Mature Secondary Structure
MISITNRPGLAIIRLPYVFRVVRMPQLILLNKPYGVICQFSEHPTHPTLKSCVPQAGVYP
CEEECCCCCEEEEEHHHHHHHHHCCEEEEEECCCCEEEECCCCCCCCCHHHHCCCCCCCC
AGRLDTDSEGLLLLTGDGALQHRIADPRWKLPKTYWVQVEGDPKEEQLEMLRRGVDLGDF
CCCCCCCCCCEEEEECCCHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCHHHH
VTRPAEVRRIETPELWPRNPPVRFRKTVPDCWLEIIISEGKNRQVRRMTAKAGLPTLRLV
HCCCHHHEECCCCCCCCCCCCCHHHHCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEE
RVAIGPWKLDGLQPGEMRAIDVRTEDLPRGASAGPGGKPGDRAPQSRSAAAAKSGKIPSF
EEEECCEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCE
RFARNRKG
ECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]