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The map label for this gene is fumA [H]

Identifier: 34498931

GI number: 34498931

Start: 3769805

End: 3771331

Strand: Reverse

Name: fumA [H]

Synonym: CV_3476

Alternate gene names: 34498931

Gene position: 3771331-3769805 (Counterclockwise)

Preceding gene: 34498932

Following gene: 34498929

Centisome position: 79.38

GC content: 64.64

Gene sequence:

>1527_bases
ATGACCATCATCCGCCAGGAAGACTTCATCCAGAGCATCGCCGATGCCTTCCAGTACATCAGTTGCTACCACCCTAAAGA
CTATATCGACGCGCTGTACCAGGCTTACCTGAACGAGGAGAGCACCGCCGCCAAGGACGCGATGGCGCAGATCCTGGTCA
ACAGCCGCATGTGCGCCGAAGGCCGCCGGCCGATCTGCCAGGATACCGGCATCGCCGTGGTGTTCCTGAAAGTGGGCATG
AAAGTGCAGTGGGACGCCCAGCTCAGCGTGCAGGAGATGGTCAACGAAGGCGTGCGCCGCGCCTACAACCACCCGGACAA
CAAGCTGCGCGCCTCCGTGCTGCTGGACCCGGCCGGCAAGCGCCAGAACTCCAAGGACAATACCCCGGCCGTGGTGCACT
TCGAGATCGTCGAAGGCGACCAGGTGGAGGTGATCTGCGCGGCCAAGGGCGGCGGCTCGGAAAACAAGTCCAAGATGGTG
ATGCTGAACCCGTCCGATTCCATCGTCGACTGGGTGCTGAAGACCGTGCCGACCATGGGCGCCGGCTGGTGTCCGCCTGG
CATTCTCGGCATCGGCATCGGCGGCACGCCGGAGAAGGCGATGCTGCTGGCGAAAGAGTCGCTGATGGACCACGTCGACA
TCCACGAGCTGAAGGCCAAGGCCGCGGCCGGCGCCGAGCTGTCCCGCGTCGAGGCGCTGCGGCTGGAGCTGTTCGACAAG
GTCAACGCGCTGGGCATCGGCGCCCAGGGCCTGGGCGGCCTGACCACGGTGCTGGACGTGAAGATCCTGGATTACCCGAC
CCACGCGGCGTCGCTGCCGGTGGCGATGATCCCCAACTGCGCCGCCACCCGCCACATCCATTTCCACCTGGACGGCAGCG
GCCCGGCCCACCTGGAAACGCCTAGCCTGGAAGACTGGCCGGACATCACCTACGACGCCTCGTCCGGCAAGCGCGTGGAC
CTGGACAAGATCACCAAGGAAGAAGTGGCCAGCTGGCAGCCGGGCGACGTGCTGCTGCTGAACGGCAAGATCCTGACCGG
CCGCGACGCCGCGCACAAGCGCATGATCGATATGCTGAACAAGGGCGAGAAGCTGCCGGTGGACTTCACCAACCGCTTCA
TCTACTACGTCGGCCCGGTCGATCCGGTGCGAGACGAAGTCGTCGGCCCGGCCGGCCCGACCACCGCCACGCGGATGGAC
AAGTTCACGCGCCAGATGCTGGAGCAGACCGGCTTGCTGGGCATGATAGGCAAGGCCGAGCGCGGTCCGGCGGCGATAGA
GGCGATCAAGGACAACAAGGCGGTCTACCTGATGGCCGTCGGCGGTTCGGCTTACCTGGTGTCCAAGGCGATCAAGGCGT
CCAAGGTGGTGGGCTTCGCCGACCTGGGCATGGAGGCGATCTACGAGTTCGAGGTCAAGGACATGCCGGTGACGGTGGCG
GTGGATTCCTGTGGCACCTCGGTGCACAACACCGGCCCGGCGGAGTGGCGGGTGAAGATTGGCAAGATTCCAGTAAGCCA
AATTTAA

Upstream 100 bases:

>100_bases
CTAGGTTTTTAGTTGTCGCAAGGCGCAGGCAGATCGGTGCGCCGGCGGCGGGGTAATTCACGGGCGGCCGCACCGGCGCC
CGGTTTCTCGAGACAGCCTT

Downstream 100 bases:

>100_bases
TCTATCAAATAAAAGCGCCACCATTTGGTGGCGCTTTTATTTTAGTTGGCAATCCAGATGGCTGGCGGCTTGTTAAATAA
GAATAGACCACCTCCTGCGC

Product: fumarate hydratase

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 508; Mature: 507

Protein sequence:

>508_residues
MTIIRQEDFIQSIADAFQYISCYHPKDYIDALYQAYLNEESTAAKDAMAQILVNSRMCAEGRRPICQDTGIAVVFLKVGM
KVQWDAQLSVQEMVNEGVRRAYNHPDNKLRASVLLDPAGKRQNSKDNTPAVVHFEIVEGDQVEVICAAKGGGSENKSKMV
MLNPSDSIVDWVLKTVPTMGAGWCPPGILGIGIGGTPEKAMLLAKESLMDHVDIHELKAKAAAGAELSRVEALRLELFDK
VNALGIGAQGLGGLTTVLDVKILDYPTHAASLPVAMIPNCAATRHIHFHLDGSGPAHLETPSLEDWPDITYDASSGKRVD
LDKITKEEVASWQPGDVLLLNGKILTGRDAAHKRMIDMLNKGEKLPVDFTNRFIYYVGPVDPVRDEVVGPAGPTTATRMD
KFTRQMLEQTGLLGMIGKAERGPAAIEAIKDNKAVYLMAVGGSAYLVSKAIKASKVVGFADLGMEAIYEFEVKDMPVTVA
VDSCGTSVHNTGPAEWRVKIGKIPVSQI

Sequences:

>Translated_508_residues
MTIIRQEDFIQSIADAFQYISCYHPKDYIDALYQAYLNEESTAAKDAMAQILVNSRMCAEGRRPICQDTGIAVVFLKVGM
KVQWDAQLSVQEMVNEGVRRAYNHPDNKLRASVLLDPAGKRQNSKDNTPAVVHFEIVEGDQVEVICAAKGGGSENKSKMV
MLNPSDSIVDWVLKTVPTMGAGWCPPGILGIGIGGTPEKAMLLAKESLMDHVDIHELKAKAAAGAELSRVEALRLELFDK
VNALGIGAQGLGGLTTVLDVKILDYPTHAASLPVAMIPNCAATRHIHFHLDGSGPAHLETPSLEDWPDITYDASSGKRVD
LDKITKEEVASWQPGDVLLLNGKILTGRDAAHKRMIDMLNKGEKLPVDFTNRFIYYVGPVDPVRDEVVGPAGPTTATRMD
KFTRQMLEQTGLLGMIGKAERGPAAIEAIKDNKAVYLMAVGGSAYLVSKAIKASKVVGFADLGMEAIYEFEVKDMPVTVA
VDSCGTSVHNTGPAEWRVKIGKIPVSQI
>Mature_507_residues
TIIRQEDFIQSIADAFQYISCYHPKDYIDALYQAYLNEESTAAKDAMAQILVNSRMCAEGRRPICQDTGIAVVFLKVGMK
VQWDAQLSVQEMVNEGVRRAYNHPDNKLRASVLLDPAGKRQNSKDNTPAVVHFEIVEGDQVEVICAAKGGGSENKSKMVM
LNPSDSIVDWVLKTVPTMGAGWCPPGILGIGIGGTPEKAMLLAKESLMDHVDIHELKAKAAAGAELSRVEALRLELFDKV
NALGIGAQGLGGLTTVLDVKILDYPTHAASLPVAMIPNCAATRHIHFHLDGSGPAHLETPSLEDWPDITYDASSGKRVDL
DKITKEEVASWQPGDVLLLNGKILTGRDAAHKRMIDMLNKGEKLPVDFTNRFIYYVGPVDPVRDEVVGPAGPTTATRMDK
FTRQMLEQTGLLGMIGKAERGPAAIEAIKDNKAVYLMAVGGSAYLVSKAIKASKVVGFADLGMEAIYEFEVKDMPVTVAV
DSCGTSVHNTGPAEWRVKIGKIPVSQI

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1787897, Length=465, Percent_Identity=32.6881720430108, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI1790564, Length=461, Percent_Identity=31.4533622559653, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1789442, Length=275, Percent_Identity=26.9090909090909, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1789443, Length=179, Percent_Identity=31.2849162011173, Blast_Score=92, Evalue=6e-20,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 55050; Mature: 54919

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIIRQEDFIQSIADAFQYISCYHPKDYIDALYQAYLNEESTAAKDAMAQILVNSRMCAE
CCEECHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHC
GRRPICQDTGIAVVFLKVGMKVQWDAQLSVQEMVNEGVRRAYNHPDNKLRASVLLDPAGK
CCCCCCCCCCEEEEEEECCCEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
RQNSKDNTPAVVHFEIVEGDQVEVICAAKGGGSENKSKMVMLNPSDSIVDWVLKTVPTMG
CCCCCCCCCEEEEEEEECCCCEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHCCCCC
AGWCPPGILGIGIGGTPEKAMLLAKESLMDHVDIHELKAKAAAGAELSRVEALRLELFDK
CCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
VNALGIGAQGLGGLTTVLDVKILDYPTHAASLPVAMIPNCAATRHIHFHLDGSGPAHLET
HHHEEECCCCCCCCEEEEEEEEEECCCCHHCCCCEECCCCCCEEEEEEEECCCCCCCCCC
PSLEDWPDITYDASSGKRVDLDKITKEEVASWQPGDVLLLNGKILTGRDAAHKRMIDMLN
CCCCCCCCCEEECCCCCEECHHHHHHHHHHCCCCCCEEEECCEEEECCHHHHHHHHHHHC
KGEKLPVDFTNRFIYYVGPVDPVRDEVVGPAGPTTATRMDKFTRQMLEQTGLLGMIGKAE
CCCCCCEEECCCEEEEECCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCC
RGPAAIEAIKDNKAVYLMAVGGSAYLVSKAIKASKVVGFADLGMEAIYEFEVKDMPVTVA
CCCHHHHEECCCCEEEEEEECCCHHHHHHHHHHHHEEEHHHHCHHHEEEEEECCCCEEEE
VDSCGTSVHNTGPAEWRVKIGKIPVSQI
ECCCCCCCCCCCCCEEEEEEECCCCCCC
>Mature Secondary Structure 
TIIRQEDFIQSIADAFQYISCYHPKDYIDALYQAYLNEESTAAKDAMAQILVNSRMCAE
CEECHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHC
GRRPICQDTGIAVVFLKVGMKVQWDAQLSVQEMVNEGVRRAYNHPDNKLRASVLLDPAGK
CCCCCCCCCCEEEEEEECCCEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
RQNSKDNTPAVVHFEIVEGDQVEVICAAKGGGSENKSKMVMLNPSDSIVDWVLKTVPTMG
CCCCCCCCCEEEEEEEECCCCEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHCCCCC
AGWCPPGILGIGIGGTPEKAMLLAKESLMDHVDIHELKAKAAAGAELSRVEALRLELFDK
CCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
VNALGIGAQGLGGLTTVLDVKILDYPTHAASLPVAMIPNCAATRHIHFHLDGSGPAHLET
HHHEEECCCCCCCCEEEEEEEEEECCCCHHCCCCEECCCCCCEEEEEEEECCCCCCCCCC
PSLEDWPDITYDASSGKRVDLDKITKEEVASWQPGDVLLLNGKILTGRDAAHKRMIDMLN
CCCCCCCCCEEECCCCCEECHHHHHHHHHHCCCCCCEEEECCEEEECCHHHHHHHHHHHC
KGEKLPVDFTNRFIYYVGPVDPVRDEVVGPAGPTTATRMDKFTRQMLEQTGLLGMIGKAE
CCCCCCEEECCCEEEEECCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCC
RGPAAIEAIKDNKAVYLMAVGGSAYLVSKAIKASKVVGFADLGMEAIYEFEVKDMPVTVA
CCCHHHHEECCCCEEEEEEECCCHHHHHHHHHHHHEEEHHHHCHHHEEEEEECCCCEEEE
VDSCGTSVHNTGPAEWRVKIGKIPVSQI
ECCCCCCCCCCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]