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The map label for this gene is rluB [H]

Identifier: 34498841

GI number: 34498841

Start: 3679923

End: 3681362

Strand: Reverse

Name: rluB [H]

Synonym: CV_3386

Alternate gene names: 34498841

Gene position: 3681362-3679923 (Counterclockwise)

Preceding gene: 34498842

Following gene: 34498840

Centisome position: 77.48

GC content: 67.22

Gene sequence:

>1440_bases
TTGCTCGCATGGAAAGAGCAAGCACATATGTCTACAAAAGGACAACGTAGCCACGCGCGCCAGCCGGTAGCGCGGGTCAA
GGGGCGCGAGACCTCGCAGCCGCGCCAGAACGCCGGCCGCAGCCAGGGCCAGCAGCGCCAGGGCAAGAATTCCGCCGCCG
GCATCCAGCCGCGCTTCCCGCAGCAGCAGGCGCAGCCAGGCCAGCAGGCGCGCCGCGGCGGCCCGCGCCAGGCGCGCGAC
GCTTACGGCAACCCGATCATGCCGTCGCAGGACGGCGTGCCGCTGTTCAAGATGAGCCGCCGCGAGCAGGAGCAGCAGCA
ACTGAATTCGGGCAACCGCAAGCCGCAGTACCAGCGCGACAGCAACGGCGACATCAACGGCAACGTGGCGCTGGACCATG
AGCAGCGTTTCGGCAACAAGAACGCCGCGCGCCCTGCCGGCAAGGGCCGCAACCAGGCGGAAGGCAAGGCCGAGGGCCAG
CCCAGGGCGGAAGCCAAGCCGCGCGGCGACGCCAAACCCAAGCAACAGGTGCAGAAGGCCAAGAAGCTGCGGCTGCGCAG
CCCCAGCCAGGACATCAAGAACAAGGCGCAGAAGCTGAAGGAAGTGCGCGTCGATCACGCCGAAATCGAGCCGGTGCGCC
TGCAGAAGGCGCTGTCCTCGTCCGGCGTCGGCTCCCGCCGCGAGATGGAGGAGTGGATCGAGGCCGGCCTGGTCACCGTC
AACGGCAAGAAGGCCAGCCTGGGCGACAAGGTGGGGCCGCAGGACAAGGTGCTAGTCAAGGGCGACCAGATCAAGCTGAA
GTGGCCGGACCGCCTGCCGCGCGTGATCATGTACCACAAGGAAGAGGGCGAGATCGTCACCCGCGACGATCCGGAAGGCC
GCGTCACCGTGTTCGACCGCCTGCCCCAGGCCAAGTCCAGCCGTTGGGTGGCCATCGGCCGTCTGGACGTCAACACTTCC
GGCCTGCTCTTGATCACCACCAGCGGCGAGTTGGCCAACCGCATGATGCACCCCAGCTTCGAAGTGGAGCGCGAGTACTC
GGTGCGCGTGCTGGGCGAACTGACCCCGGAAATCATGAAGGAAATGACCAAGGGCGTGGAGCTGGAAGACGGCGAGGCCC
GCTTCGACCGCATCTTCCAGACCGGCGGCCAGGAAGAGTCGGCCAACCAGTGGTACAACGTGGTGATCAAGGAAGGCCGC
AATCGCGAAGTGCGCCGCATGTTCGAGCACTTCGGCCTGACCGTCAGCCGCCTGATGCGTGTGCGCTTCGGCAACATCGG
CCTGCCTCCGCGCCTGAAGCGCGGCCAGTTCTACGAGCTGAACGCCGCCGAAGTGGCCGCGGTGATGAAGTGGTCCAACC
TGACCGTGACCGGCGGCAAGAAGGCCGGCGGCCAGCGCCGCGGATCCCCCCGCAAGCCCAAGGCCGCGCGCAGCGAGTAA

Upstream 100 bases:

>100_bases
CCAAACACAGGACGACAGCCCCGATGCGGGCGCAGACGATATCCCCCTCGACGATGAGGCGGACAATTTAGAGCCAGTCG
CGGAATAACGCTTCCGCCAG

Downstream 100 bases:

>100_bases
GCCGGCCGTTCCGCGAAAGCGGAACCCGCTTCCAAGACATGGGCGACCGCCGGCTGGCGGTTGCCCGTTTTTGTTTTCCG
GGAGGTCAGTTGGAAGAATT

Product: hypothetical protein

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MLAWKEQAHMSTKGQRSHARQPVARVKGRETSQPRQNAGRSQGQQRQGKNSAAGIQPRFPQQQAQPGQQARRGGPRQARD
AYGNPIMPSQDGVPLFKMSRREQEQQQLNSGNRKPQYQRDSNGDINGNVALDHEQRFGNKNAARPAGKGRNQAEGKAEGQ
PRAEAKPRGDAKPKQQVQKAKKLRLRSPSQDIKNKAQKLKEVRVDHAEIEPVRLQKALSSSGVGSRREMEEWIEAGLVTV
NGKKASLGDKVGPQDKVLVKGDQIKLKWPDRLPRVIMYHKEEGEIVTRDDPEGRVTVFDRLPQAKSSRWVAIGRLDVNTS
GLLLITTSGELANRMMHPSFEVEREYSVRVLGELTPEIMKEMTKGVELEDGEARFDRIFQTGGQEESANQWYNVVIKEGR
NREVRRMFEHFGLTVSRLMRVRFGNIGLPPRLKRGQFYELNAAEVAAVMKWSNLTVTGGKKAGGQRRGSPRKPKAARSE

Sequences:

>Translated_479_residues
MLAWKEQAHMSTKGQRSHARQPVARVKGRETSQPRQNAGRSQGQQRQGKNSAAGIQPRFPQQQAQPGQQARRGGPRQARD
AYGNPIMPSQDGVPLFKMSRREQEQQQLNSGNRKPQYQRDSNGDINGNVALDHEQRFGNKNAARPAGKGRNQAEGKAEGQ
PRAEAKPRGDAKPKQQVQKAKKLRLRSPSQDIKNKAQKLKEVRVDHAEIEPVRLQKALSSSGVGSRREMEEWIEAGLVTV
NGKKASLGDKVGPQDKVLVKGDQIKLKWPDRLPRVIMYHKEEGEIVTRDDPEGRVTVFDRLPQAKSSRWVAIGRLDVNTS
GLLLITTSGELANRMMHPSFEVEREYSVRVLGELTPEIMKEMTKGVELEDGEARFDRIFQTGGQEESANQWYNVVIKEGR
NREVRRMFEHFGLTVSRLMRVRFGNIGLPPRLKRGQFYELNAAEVAAVMKWSNLTVTGGKKAGGQRRGSPRKPKAARSE
>Mature_479_residues
MLAWKEQAHMSTKGQRSHARQPVARVKGRETSQPRQNAGRSQGQQRQGKNSAAGIQPRFPQQQAQPGQQARRGGPRQARD
AYGNPIMPSQDGVPLFKMSRREQEQQQLNSGNRKPQYQRDSNGDINGNVALDHEQRFGNKNAARPAGKGRNQAEGKAEGQ
PRAEAKPRGDAKPKQQVQKAKKLRLRSPSQDIKNKAQKLKEVRVDHAEIEPVRLQKALSSSGVGSRREMEEWIEAGLVTV
NGKKASLGDKVGPQDKVLVKGDQIKLKWPDRLPRVIMYHKEEGEIVTRDDPEGRVTVFDRLPQAKSSRWVAIGRLDVNTS
GLLLITTSGELANRMMHPSFEVEREYSVRVLGELTPEIMKEMTKGVELEDGEARFDRIFQTGGQEESANQWYNVVIKEGR
NREVRRMFEHFGLTVSRLMRVRFGNIGLPPRLKRGQFYELNAAEVAAVMKWSNLTVTGGKKAGGQRRGSPRKPKAARSE

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=235, Percent_Identity=55.7446808510638, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1790453, Length=240, Percent_Identity=33.75, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1788510, Length=213, Percent_Identity=29.1079812206573, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87081838, Length=168, Percent_Identity=29.7619047619048, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 53813; Mature: 53813

Theoretical pI: Translated: 11.15; Mature: 11.15

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAWKEQAHMSTKGQRSHARQPVARVKGRETSQPRQNAGRSQGQQRQGKNSAAGIQPRFP
CCCCCHHHHCCCCCCHHHHHCHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
QQQAQPGQQARRGGPRQARDAYGNPIMPSQDGVPLFKMSRREQEQQQLNSGNRKPQYQRD
HHHCCCCHHHHCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
SNGDINGNVALDHEQRFGNKNAARPAGKGRNQAEGKAEGQPRAEAKPRGDAKPKQQVQKA
CCCCCCCCEEECHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
KKLRLRSPSQDIKNKAQKLKEVRVDHAEIEPVRLQKALSSSGVGSRREMEEWIEAGLVTV
HHHHCCCCHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHCCCCCHHHHHHHHHCCEEEE
NGKKASLGDKVGPQDKVLVKGDQIKLKWPDRLPRVIMYHKEEGEIVTRDDPEGRVTVFDR
CCCCCCCCCCCCCCCCEEEECCEEEEECCCCCCCEEEEECCCCCEEECCCCCCCEEHEEC
LPQAKSSRWVAIGRLDVNTSGLLLITTSGELANRMMHPSFEVEREYSVRVLGELTPEIMK
CCCCCCCCEEEEEEEEECCCCEEEEECCHHHHHHHCCCCCCCCCCCHHHHHHHCCHHHHH
EMTKGVELEDGEARFDRIFQTGGQEESANQWYNVVIKEGRNREVRRMFEHFGLTVSRLMR
HHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHEEHHEECCCCHHHHHHHHHHCHHHHHHHH
VRFGNIGLPPRLKRGQFYELNAAEVAAVMKWSNLTVTGGKKAGGQRRGSPRKPKAARSE
HHHCCCCCCCCCCCCCEEECCHHHHHHHHEECCEEEECCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLAWKEQAHMSTKGQRSHARQPVARVKGRETSQPRQNAGRSQGQQRQGKNSAAGIQPRFP
CCCCCHHHHCCCCCCHHHHHCHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
QQQAQPGQQARRGGPRQARDAYGNPIMPSQDGVPLFKMSRREQEQQQLNSGNRKPQYQRD
HHHCCCCHHHHCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
SNGDINGNVALDHEQRFGNKNAARPAGKGRNQAEGKAEGQPRAEAKPRGDAKPKQQVQKA
CCCCCCCCEEECHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
KKLRLRSPSQDIKNKAQKLKEVRVDHAEIEPVRLQKALSSSGVGSRREMEEWIEAGLVTV
HHHHCCCCHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHCCCCCHHHHHHHHHCCEEEE
NGKKASLGDKVGPQDKVLVKGDQIKLKWPDRLPRVIMYHKEEGEIVTRDDPEGRVTVFDR
CCCCCCCCCCCCCCCCEEEECCEEEEECCCCCCCEEEEECCCCCEEECCCCCCCEEHEEC
LPQAKSSRWVAIGRLDVNTSGLLLITTSGELANRMMHPSFEVEREYSVRVLGELTPEIMK
CCCCCCCCEEEEEEEEECCCCEEEEECCHHHHHHHCCCCCCCCCCCHHHHHHHCCHHHHH
EMTKGVELEDGEARFDRIFQTGGQEESANQWYNVVIKEGRNREVRRMFEHFGLTVSRLMR
HHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHEEHHEECCCCHHHHHHHHHHCHHHHHHHH
VRFGNIGLPPRLKRGQFYELNAAEVAAVMKWSNLTVTGGKKAGGQRRGSPRKPKAARSE
HHHCCCCCCCCCCCCCEEECCHHHHHHHHEECCEEEECCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]