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The map label for this gene is ycdT [H]

Identifier: 34498537

GI number: 34498537

Start: 3357341

End: 3358357

Strand: Direct

Name: ycdT [H]

Synonym: CV_3082

Alternate gene names: 34498537

Gene position: 3357341-3358357 (Clockwise)

Preceding gene: 34498536

Following gene: 34498540

Centisome position: 70.66

GC content: 65.09

Gene sequence:

>1017_bases
ATGCCCCACTCCGCCTCGAAGCAGGACGACAGCAAGCTTCTGCTCGACAATCTGGTCGAGCTGACCTCGCAGCGCGAGCA
GGAAATGCTGGAGTCCAGCCTGCTGTCCACGCTGGTGGAGCTGATGCGGGTGGACAAGGCCGAACTGTTCGCCTGCCGCT
GGGTCAACGGCGCGCCCTACATCCGCCGCAGGCTGGAAGCCCAGGCCTCCTCCGGCAAACCCGTCGTGCGGGAAACCAAC
ACCGACGGCTGGCTGCCGCCCCCCGCCTTCCTGTACGACCTGCTCAACCGCGTCGGCCGCGACGACGTGGTGCAGCGCAT
CCCCAGCGGCCTGTGCCTGCCGCTGCGCTGCATGGGCAACATCATCGCCCTGGTGGTGATCCACTGCGGCAGCGACTCCA
GGATCAACCGCGACATGCTCAAGGCGATGACGCGGATACACGAGAACTTCTTGCGCCTGCTGTTCGAGGCCGACCGTGAC
ATGCTGACCGCGCTGCACAACCGCCGCAAGTTCGACCTGCGCTTCTACAACCTGATCGCCGCGCTGCAGCACGAGCCCGC
ATCCACGATGCCGCACGTGCTGGCGTTGCTGGACATCGACCATTTCAAACGGGTGAACGACCAATTCGGCCACATGGTCG
GCGACGAGGTGCTGCTGCTGATGGCGCAACTGATGCAGCAGAGCTTCCGCGAGGAAGACGGGCTGTTCCGCTACGGCGGC
GAGGAGTTCGCGCTGCTCCTGCAAGGCAACCAGCCGGAAATCGCCGAACAGGCGCTGGAACGCTTCCGCGCCACGGTGGA
AGGCCACATCTTCCCGCAAGTCGGCAACATCACCATCAGCATCGGGCATACCCGCATCGTCCACGGCCAACTGCCCGACC
GCCTGATCGAGCAAGCCGACCGCGCGCTTTATTACGCCAAGAGCCATGGCCGCAACCAGGTGCGCGGCTACGAACAGTTG
GAAGCCGGCGGCGAGCTGGAGGCGCCGCCGGCTTCCAGCCAGGTCGACTATTTCTGA

Upstream 100 bases:

>100_bases
TCGGCAGCACCGGCCGCGGCTGAGGCGTTTTTCCCGGCGGGCTTGCCTATAATTCCGTGGACCCGCGGCGCCGTCGCGCC
GCGGCACAGGAGCGCCCGCC

Downstream 100 bases:

>100_bases
ACGCCGGTGGTCACGGCAACTGGACGATGCGGTCAAGCGCCGGCACCGGCAGCGCGGGATGCGCCGCCAGATAGCTTTGC
AGCACGTCCACGTCCACCCC

Product: GGDEF family regulatory protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MPHSASKQDDSKLLLDNLVELTSQREQEMLESSLLSTLVELMRVDKAELFACRWVNGAPYIRRRLEAQASSGKPVVRETN
TDGWLPPPAFLYDLLNRVGRDDVVQRIPSGLCLPLRCMGNIIALVVIHCGSDSRINRDMLKAMTRIHENFLRLLFEADRD
MLTALHNRRKFDLRFYNLIAALQHEPASTMPHVLALLDIDHFKRVNDQFGHMVGDEVLLLMAQLMQQSFREEDGLFRYGG
EEFALLLQGNQPEIAEQALERFRATVEGHIFPQVGNITISIGHTRIVHGQLPDRLIEQADRALYYAKSHGRNQVRGYEQL
EAGGELEAPPASSQVDYF

Sequences:

>Translated_338_residues
MPHSASKQDDSKLLLDNLVELTSQREQEMLESSLLSTLVELMRVDKAELFACRWVNGAPYIRRRLEAQASSGKPVVRETN
TDGWLPPPAFLYDLLNRVGRDDVVQRIPSGLCLPLRCMGNIIALVVIHCGSDSRINRDMLKAMTRIHENFLRLLFEADRD
MLTALHNRRKFDLRFYNLIAALQHEPASTMPHVLALLDIDHFKRVNDQFGHMVGDEVLLLMAQLMQQSFREEDGLFRYGG
EEFALLLQGNQPEIAEQALERFRATVEGHIFPQVGNITISIGHTRIVHGQLPDRLIEQADRALYYAKSHGRNQVRGYEQL
EAGGELEAPPASSQVDYF
>Mature_337_residues
PHSASKQDDSKLLLDNLVELTSQREQEMLESSLLSTLVELMRVDKAELFACRWVNGAPYIRRRLEAQASSGKPVVRETNT
DGWLPPPAFLYDLLNRVGRDDVVQRIPSGLCLPLRCMGNIIALVVIHCGSDSRINRDMLKAMTRIHENFLRLLFEADRDM
LTALHNRRKFDLRFYNLIAALQHEPASTMPHVLALLDIDHFKRVNDQFGHMVGDEVLLLMAQLMQQSFREEDGLFRYGGE
EFALLLQGNQPEIAEQALERFRATVEGHIFPQVGNITISIGHTRIVHGQLPDRLIEQADRALYYAKSHGRNQVRGYEQLE
AGGELEAPPASSQVDYF

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=166, Percent_Identity=39.7590361445783, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87081881, Length=203, Percent_Identity=33.0049261083744, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1787816, Length=164, Percent_Identity=35.9756097560976, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI145693134, Length=165, Percent_Identity=36.969696969697, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786584, Length=178, Percent_Identity=32.5842696629214, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788085, Length=167, Percent_Identity=37.125748502994, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1788381, Length=213, Percent_Identity=32.8638497652582, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI87082007, Length=168, Percent_Identity=35.7142857142857, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787056, Length=196, Percent_Identity=30.1020408163265, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787802, Length=166, Percent_Identity=32.5301204819277, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081974, Length=241, Percent_Identity=26.5560165975104, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788956, Length=168, Percent_Identity=30.952380952381, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787541, Length=168, Percent_Identity=30.952380952381, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 38456; Mature: 38324

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHSASKQDDSKLLLDNLVELTSQREQEMLESSLLSTLVELMRVDKAELFACRWVNGAPY
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEECCCCHH
IRRRLEAQASSGKPVVRETNTDGWLPPPAFLYDLLNRVGRDDVVQRIPSGLCLPLRCMGN
HHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCHHHHHHHCCCCCCHHHHHHHH
IIALVVIHCGSDSRINRDMLKAMTRIHENFLRLLFEADRDMLTALHNRRKFDLRFYNLIA
HHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ALQHEPASTMPHVLALLDIDHFKRVNDQFGHMVGDEVLLLMAQLMQQSFREEDGLFRYGG
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
EEFALLLQGNQPEIAEQALERFRATVEGHIFPQVGNITISIGHTRIVHGQLPDRLIEQAD
CEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECCEEEEECCEEEEECCCHHHHHHHHH
RALYYAKSHGRNQVRGYEQLEAGGELEAPPASSQVDYF
HHHHHHHHCCCHHHCCHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PHSASKQDDSKLLLDNLVELTSQREQEMLESSLLSTLVELMRVDKAELFACRWVNGAPY
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEECCCCHH
IRRRLEAQASSGKPVVRETNTDGWLPPPAFLYDLLNRVGRDDVVQRIPSGLCLPLRCMGN
HHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCHHHHHHHCCCCCCHHHHHHHH
IIALVVIHCGSDSRINRDMLKAMTRIHENFLRLLFEADRDMLTALHNRRKFDLRFYNLIA
HHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ALQHEPASTMPHVLALLDIDHFKRVNDQFGHMVGDEVLLLMAQLMQQSFREEDGLFRYGG
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
EEFALLLQGNQPEIAEQALERFRATVEGHIFPQVGNITISIGHTRIVHGQLPDRLIEQAD
CEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECCEEEEECCEEEEECCCHHHHHHHHH
RALYYAKSHGRNQVRGYEQLEAGGELEAPPASSQVDYF
HHHHHHHHCCCHHHCCHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]