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The map label for this gene is dut

Identifier: 34498536

GI number: 34498536

Start: 3356811

End: 3357263

Strand: Direct

Name: dut

Synonym: CV_3081

Alternate gene names: 34498536

Gene position: 3356811-3357263 (Clockwise)

Preceding gene: 34498535

Following gene: 34498537

Centisome position: 70.65

GC content: 68.65

Gene sequence:

>453_bases
ATGCAAGCCGTTATCGACGTCAAGATCCTGGACGCGCGCCTGCGCGACAACCTGCCCGCCTACGCCACCGCCGGCTCGGC
CGGACTGGATCTGCGCGCCGCCACCGAAGAGACGATGACCATCCAGCCGGGCGAAACCCAGCTGGTTCCGACCGGCATCG
CCATCCACCTGAGCGATCCGGGCCTGGCTGCGATGCTGCTGCCGCGCTCCGGCCTGGGCCACAAGCACGGCATCGTGCTG
GGCAACCTGGTGGGCCTGATCGACTCCGATTACCAGGGCCAGATGTTCGTGTCCGTGTGGAACCGCGGCCAGCAGCCGTT
CCGCCTGGAGCCGATGGAGCGCATCGCCCAGATGGTGATCGTGCCGGTGGTCCAGGCCAGCTTCAACATCGTCGACGACT
TCGACGCGTCCGACCGCGGCGCCGGCGGTTTCGGCAGCACCGGCCGCGGCTGA

Upstream 100 bases:

>100_bases
CCTCGGCCATCGTCCGCCACCTGGCCGCCCTGCTGGCCAAACGCTGAAGAATCAACCATGCGGCGGCCAGCCGGCCGCCG
CCGCATCACAAGGAAACACC

Downstream 100 bases:

>100_bases
GGCGTTTTTCCCGGCGGGCTTGCCTATAATTCCGTGGACCCGCGGCGCCGTCGCGCCGCGGCACAGGAGCGCCCGCCATG
CCCCACTCCGCCTCGAAGCA

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 150; Mature: 150

Protein sequence:

>150_residues
MQAVIDVKILDARLRDNLPAYATAGSAGLDLRAATEETMTIQPGETQLVPTGIAIHLSDPGLAAMLLPRSGLGHKHGIVL
GNLVGLIDSDYQGQMFVSVWNRGQQPFRLEPMERIAQMVIVPVVQASFNIVDDFDASDRGAGGFGSTGRG

Sequences:

>Translated_150_residues
MQAVIDVKILDARLRDNLPAYATAGSAGLDLRAATEETMTIQPGETQLVPTGIAIHLSDPGLAAMLLPRSGLGHKHGIVL
GNLVGLIDSDYQGQMFVSVWNRGQQPFRLEPMERIAQMVIVPVVQASFNIVDDFDASDRGAGGFGSTGRG
>Mature_150_residues
MQAVIDVKILDARLRDNLPAYATAGSAGLDLRAATEETMTIQPGETQLVPTGIAIHLSDPGLAAMLLPRSGLGHKHGIVL
GNLVGLIDSDYQGQMFVSVWNRGQQPFRLEPMERIAQMVIVPVVQASFNIVDDFDASDRGAGGFGSTGRG

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=140, Percent_Identity=38.5714285714286, Blast_Score=85, Evalue=3e-17,
Organism=Homo sapiens, GI4503423, Length=140, Percent_Identity=38.5714285714286, Blast_Score=84, Evalue=4e-17,
Organism=Homo sapiens, GI70906441, Length=140, Percent_Identity=38.5714285714286, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1790071, Length=147, Percent_Identity=70.7482993197279, Blast_Score=218, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI71988561, Length=146, Percent_Identity=36.986301369863, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6319729, Length=153, Percent_Identity=36.6013071895425, Blast_Score=79, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19921126, Length=148, Percent_Identity=32.4324324324324, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24583610, Length=148, Percent_Identity=32.4324324324324, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_CHRVO (Q7MBE8)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_902751.1
- ProteinModelPortal:   Q7MBE8
- SMR:   Q7MBE8
- GeneID:   2549905
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_3081
- NMPDR:   fig|243365.1.peg.3081
- HOGENOM:   HBG436079
- OMA:   LDLRACI
- PhylomeDB:   Q7MBE8
- ProtClustDB:   PRK00601
- BioCyc:   CVIO243365:CV_3081-MONOMER
- BRENDA:   3.6.1.23
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 15941; Mature: 15941

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: BINDING 82-82

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAVIDVKILDARLRDNLPAYATAGSAGLDLRAATEETMTIQPGETQLVPTGIAIHLSDP
CCEEEEEEEEEHHHHCCCCCEEECCCCCCEEEECCCCEEEECCCCCEEEECCEEEEECCC
GLAAMLLPRSGLGHKHGIVLGNLVGLIDSDYQGQMFVSVWNRGQQPFRLEPMERIAQMVI
CEEEEEECCCCCCCCCCEEEHHHHHHCCCCCCCEEEEEEECCCCCCEEECHHHHHHHHHH
VPVVQASFNIVDDFDASDRGAGGFGSTGRG
HHHHHCCCCEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQAVIDVKILDARLRDNLPAYATAGSAGLDLRAATEETMTIQPGETQLVPTGIAIHLSDP
CCEEEEEEEEEHHHHCCCCCEEECCCCCCEEEECCCCEEEECCCCCEEEECCEEEEECCC
GLAAMLLPRSGLGHKHGIVLGNLVGLIDSDYQGQMFVSVWNRGQQPFRLEPMERIAQMVI
CEEEEEECCCCCCCCCCEEEHHHHHHCCCCCCCEEEEEEECCCCCCEEECHHHHHHHHHH
VPVVQASFNIVDDFDASDRGAGGFGSTGRG
HHHHHCCCCEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782